Phylogeny, Antimicrobial, Antioxidant and Enzyme Producing Potentialof Fungal Endophytes

Phylogeny, antimicrobial, antioxidant and enzyme producing potentialof fungal endophytes found in Viola odorata

Meenu Katoch1*, Arshia Singh1, Gurpreet Singh1, Priya Wazir2, Rajinder Kumar1

1Microbial Biotechnology Department,2Instrumentation Division,

CSIR- Indian Institute of Integrative Medicine, Canal Road, Jammu Tawi, Jammu 180 001, India

Supplementary data

Fig S1: Agarose gel electrophoresis (1.5%) of polymerase chain reaction product lane (1) 1Kb DNA ladder (2) amplified product of VOR16. Primers used were based on Internal transcribed spacer (ITS)-5.8S ribosomal gene. The approximately (600) bp bandin lane (2) indicative of (ITS)-5.8S ribosomal gene amplification

Fig S2: Plates showing the antimicrobial activity of endophytes against Pseudomonas aeruginosa; Staphylococcus aureus; Klebsiella pneumoniaeby agar disc diffusion assay.

Fig S3: Calibration curve of Penicillin G

Fig S4: LC-MS chromatogram showing Penicillin G; B: Scan of m/z 114

Table S1: Penicillin G content in the extract of endophytes showing significant antimicrobial activity against human pathogens

Fig S5: Calibration curve for phenolics content

Fig S6: Calibration curve for flavonoid content

Fig S7: Plates showing zone of clearance due to production of the protease, amylase, glutenase and lipase enzymes by endophytes on potato dextrose agar plates containing specific substrates

Fig S1: Agarose gel electrophoresis (1.5%) of polymerase chain reaction product lane (1) 1Kb DNA ladder (2) amplified product of VOR16. Primers used were based on Internal transcribed spacer (ITS)-5.8S ribosomal gene. The approximately (600) bp bandin lane (2) indicative of (ITS)-5.8S ribosomal gene amplification

Fig S2:Endophytes showing the antimicrobial activity against Pseudomonas aeruginosa; Staphylococcus aureus; Klebsiella pneumoniaeby agar disc diffusion assay

Fig S3: Calibration curve of Penicilline G

Fig S4: LC-MS chromatogram showing Penicilline G; B: MS Scan of m/z 114

Table S1: Penicillin G content in the extract of endophytes showing significant antimicrobial activity against human pathogens

S No. / Endophyte / RT / Area / Concentration (ng/mg)
1 / VOR18 / 0.576 / 11911 / 45.7
2 / VOR16 / 0.754 / 10964 / 42.068
3 / VOLN1 / 0.751 / 5812 / 22.3
4 / VOR12 / ND / - / -

Fig. S5:Calibration curve for phenolics content

Fig. S6:Calibration curve for flavonoid content

Fig S7: Plates showing zone of clearance due to production of the protease, amylase, glutenase and Lipase enzymes by endophytes on potato dextrose agar plates containing specific substrates