Table S1 Primers used in this study

Name / Sequence（5’-3’） / Purpose
RoTFL1c-F / TCTTTGATATTTGTACGACCTTCTCTC / RoTFL1c-cDNA
RoTFL1c-R / ACCGTTATAGCATGGAATCTTTTG
RoTFL1c-ORF-F / CCAACGCAAAACACACATTCATCTTCTG / RoTFL1c-ORF
RoTFL1c-ORF-R / GAACATGTCCACAACCTCTCCTACCACTCT
RoTFL1c-XbaⅠ-ORF-F / TCTAGAGATATTTGTACGACCTTCTCTCC / RoTFL1c-OE
RoTFL1c-SacⅠ-ORF-R / GAGCTCGAGCTCCATTCACGTACAGCTCTCTTTG
RoTFL1c-CF / TGCAGTCACCGCCAAACCTA / RoTFL1c-genomic DNA PCR
RoTFL1c-CR / AACGGCGGCAACAGGAAGA
RoTFL1c-RT-F / TTTGATATTTGTACGACCTTCTCTCCTCTCTC / RoTFL1c real-time PCR
RoTFL1c-RT-R / CATTGAAGACGAGTTTGGTGTTGTAAGTGAC
Actin-At-F / TCTCTATGCCAGTGGTCGTA / ACTIN internal control in real-time PCR
Actin-At-R / CCTCAGGACAACGGAATC

Table S2 Statistics of RoTFL1c-OE transgenic Arabidopsis thaliana with various phenotypes

Class / First batch / Second batch / Third batch
Number of plants / Percentage（%） / Number of plants / Percentage（%） / Number of plants / Percentage（%）
I / 1 / 11.1 / 3 / 17.7 / 1 / 8.3
II / 2 / 22.2 / 4 / 23.5 / 3 / 25.0
III / 3 / 33.3 / 5 / 29.4 / 4 / 33.3
IV / 3 / 33.3 / 5 / 29.4 / 4 / 33.3

Note: Phenotypes in Class I: clustered branches; Class II: a significant number of rosette leaves; Class III: no blossoms; and Class IV: delayed flowering.

Figure S1 Electrophoresis analysis of the RoTFL1c ORF fragment. M: DNA ladder Marker II, 1: the cloned RoTFL1c ORF fragment , 2: blank control.


A

B

Figure S2 Sequence alignment (A) and phylogenic tree (B) of TFL1 protein homologues in Rosaceae and Arabidopsis thaliana. The TFL1 homologous proteins analyzed in the tree are as follows: MdTFL1 (NP_001280887.1) from Malus domestica, PypTFL1 (BAD10962.1) from Pyrus pyrifolia, PbTFL1 (XP_009374732.1) from Pyrus × bretschneideri, PsTFL1 (BAD10965.1) from Pseudocydonia sinensis, EjTFL1 (BAD10966.1) from Eriobotrya japonica, MpTFL1 (ACF18104.1) from Malus prattii, CoTFL1 (BAD10964.1) from Cydonia oblonga, PfTFL1 (AEO72026.1) from Pyracantha fortuneana, PyTFL1 (AEO72023.1) from Prunus yedoensis, PpTFL1 (XP_007202664.1) from Prunus persica, PmTFL1 (XP_008241250.1) from Prunus mume, PaTFL1 (ADL62862.1) from Prunus armeniaca, RmTFL1c from Rosa multiflora ‘Albo-plena’, RoKSN (ADO64261.1) from Rosa chinensis var. spontanea, FaTFL1 (AFA42328.1) from Fragaria × ananassa, FvTFL1 (NP_001267006.1) from Fragaria vesca, ScTFL1 (AEO72025.1) from Spiraea cantoniensis, MfTFL1 (ACF18112.1) from Malus floribunda, PseTFL1 (AEO72024.1) from Photinia serratifolia, MbTFL1 (ACF18111.1) from Malus baccata, and MzTFL1 (ACF18109.1) from Malus zumi.

Figure S3 Sketch map of the plant overexpression vector pBI121-RoTFL1c.

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