MAJOR EQUIPMENT
Specific Fields Relevant for Robert P. Apkarian Integrated EM Core Users
Transmission Electron Microscopy (TEM):
· JEOL JEM-1400 120 kV LaB6 TEM with Gatan CCD cameras. Capable of all modes of TEM, including tomography of sectioned materials.
o Accelerating voltages: 40 kV - 120 kV
o LaB6 filament
o Gatan CCD cameras
o Negative sheet film
o Image orientation system
o Motorized goniometer allows +/- 80° sample tilting
o Minimum Dose System (MDS) allows for imaging of beam-sensitive samples
o SerialEM for semi-automated data acquisition
o Beam blocker for electron diffraction work
· Hitachi HT-7700 120 kV W (Tungsten) TEM with AMT CCD camera. Capable of all modes of TEM, including tomography and electron diffraction.
o Accelerating voltages: 40 kV - 120 kV
o W (Tungsten) filament
o AMT CCD camera
o Image orientation system
o Motorized goniometer allows +/- 70° sample tilting
o Low Dose System allows for imaging of beam-sensitive samples
o SerialEM for semi-automated data acquisition
o Beam blocker for electron diffraction work
Scanning Electron Microscopy (SEM):
· Topcon DS-130F Field Emission SEM/STEM capable of in-lens and below-lens conventional SEM, and in-lens cryo-HRSEM.
· Topcon DS-150F Field Emission SEM /STEM with BSE (back-scattered electron detection) and capable of in-lens and below-lens conventional SEM, and in-lens cryo-HRSEM.
o Accelerating voltages: 0.5 kV - 30 kV
o Schottky field emission sources
o BSE and STEM systems
o Gatan CT-3500 cold stage
o In-lens and below-lens imaging system
Sample Preparation:
· Denton DV-602 Turbo Magnetron Sputter system with a chromium target
· EMSCOPE SC-500 Magnetron Sputter system with a gold target
· Denton Benchtop Turbo Carbon Evaporator
· Leica UC6rt and Ultracut S Ultramicrotomes
· RMC PT-XL Ultramicrotome
· RMC BAL-TEC HPM 010 High Pressure Freezing machine and Leica AFS cryo-substitution apparatus
· Leica UC6i/FC6 Cryo-Ultramicrotome
Imaging Data Processing and Analysis:
· 3 iMac workstations with Adobe, ImageJ, and IMOD software packages. Other image processing software packages installed as needed.