SM Fig. S2

a

GATATTGCACTCATTTCGGAGACGCATTTCTCGAACAAGAACTTCATTAA 0050

ATTCAAAGGCTACTGCACCTATTGGACTACCCACCCGAGTGAGCGAGCCC 0100

GCGGAGGTACATCAATACTGATCAAACAAAATATTGAACATCACCTGCAA 0150

GACGACATACGGGAAACATATATTCAGGCTACAATTGTTTGCATTCAACA 0200

TAGTGGGACTGAATTAAATGTTGGGGCAGTATACTGCCCTCCTAGACACA 0250

CAATAACTAAAACACAATATCTTAGTATTTTCAACAAATTGGGATCATCC 0300

TGGGGGGAGACTTCAACGTAAAACACACAGCTTGGGGATCAAGACTGATT 0350

ACTCCAAATAAAGGCAGCCAGTTATTATCGGCCATTAATGAGGAAAAATG 0400

CGGATTCCACTCATCAGGAAAGCCTACGTACTGGCCAACTGACAACAAAA 0450

AAGTTTCGGATTTGATCGACTTCTTCATCTCTAAAGGAATCAATAATAAT 0500

TGCTGCTCACAGAGGGAATAGAAGATCTATCGTCAGATCATACCCCAGTA 0550

CTCCTGATTATTACTAGTGAAGTCATCAAAAAAGTGAAGAAACAAAATCT 0600

CACAACCAAACGTACCAACTGGGAAACTTTCAAGAATATCTGGATGAACT 0650

AATTGATCTGACGTTAACTGAGAGACAAAGATGAGCTAGAGAGTGCTGCT 0700

CAATATTTATCAACTGGATACAAATGGCTGCAAAAAAGGCAACTCCAGCT 0750

CCATGTGACAAACCAGAATACGAAACCAATTACCCAACTGAAATTAGAGA 0800

GCTAATCAAGGAACGGCGCAAATTATGACGAATATGGCACGGATCCAGAC 0850

ATCCTTCAGATAAAAGGAACTTCAATGTGGTGAGCAATCGTGTTAACTGA 0900

CTTATCAAAGAATTCAAGCAGAGCTGCATTAGTGAATACCTCAACGGATT 0950

GGGACCAGGAGCCGAAAAAGAATATTCTCTATGGAAAGCCACAAGACGAC 1000

TTAAAAGACCAACAGTCCAAGTGCCGCCAATCAAAATATGCACAAGGCCA 1050

ATGGATTAGAAAGGATAAAGAAAAAGCTGAACTCTTTGCCCAGTATCTTT 1100

CGAGTGTATTTCAACCACATGAAATAAACTCGAACATCAGCACGGCTCCA 1150

GAATACCT

b

Rhynchosciara americana non-LTR retrotransposon RaTART, complete

sequence Length=8268

Score = 1509 bits (817), Expect = 0.0

Identities = 907/947 (95%), Gaps = 19/947 (2%)

Strand=Plus/Minus

Probe 1 229 GTATACTGCCCTCCTAGACACACAATAACTAAAACACAATATCTTAG-TATTTTCAACAA 287

|||||||| |||||||||| ||||||||||||||||||||||||| | ||||||||||||

RaTART 1048 GTATACTGTCCTCCTAGACGCACAATAACTAAAACACAATATCTT-GATATTTTCAACAA 990

Probe 1 288 A-TTGG-----GA-TCATCCTGGGGGGAGACTTCAACGTAAAACACACAGCTTGGGGATC 340

| |||| | |||||||||||| ||||||||||||||||||||||||||||| |||

RaTART 989 ATTTGGATCAAAATTCATCCTGGGGGCAGACTTCAACGTAAAACACACAGCTTGGGAATC 930

Probe 1 341 AAGACTGATTACTCCAAATAAAGGCAGCCAGTTATTATCGGCCATTAATGAGGAAAAATG 400

||||||||||||||||||||||||||| || |||||||||||||||||||||| ||||||

RaTART 929 AAGACTGATTACTCCAAATAAAGGCAGTCAATTATTATCGGCCATTAATGAGGGAAAATG 870

Probe 1 401 CGGATTCCACTCATCAGGAAAGCCTACGTACTGGCCAACTGACAACAAAAAAGTTTCGGA 460

||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||

RaTART 869 CGGATTCCACTCATCAGGAAAGCCTACGTACTGGCCAACTGACAACAAAAAAGTTCCGGA 810

Probe 1 461 TTTGATCGACTTCTTCATCTCTAAAGGAATCAATAATAATTGCTGCTC-ACAGAGGGAAT 519

|||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||

RaTART 809 TTTGATCGACTTCTTCATCTCTAAAGGAATCAATAATAATTGCTGCTCAACAGAGGGAAT 750

Probe 1 520 AGAAGATCTATCGTCAGATCATACCCCAGTACTCCTGATTATTACTAGTGAAGTCATCAA 579

||||||||||||||||||||| |||||||||||||||||||||| |||| ||||||||||

RaTART 749 AGAAGATCTATCGTCAGATCACACCCCAGTACTCCTGATTATTAGTAGTAAAGTCATCAA 690

Probe 1 580 AAAAGTGAAGAAACAAAATCTCACAACCAAACGTACCAACTGGGAAACTTTCA-AGAATA 638

|||||||||||||||||||||||||||||||||||| |||||||||||||||| ||||||

RaTART 689 AAAAGTGAAGAAACAAAATCTCACAACCAAACGTACAAACTGGGAAACTTTCAGAGAATA 630

Probe 1 639 TCTGGATGAACTAATTGATCTG-ACGTT-AACTGAGAGACAAAGATGAGCTAGAGAGTGC 696

| |||||||||||||||||||| ||||| |||||||||||||||||||||||||||||||

RaTART 629 TTTGGATGAACTAATTGATCTGAACGTTAAACTGAGAGACAAAGATGAGCTAGAGAGTGC 570

Probe 1 697 TGCTC-AATATTTATCAACTGGATACAAATGGCTGCAAAAAAGGCAACTCCAGCTCCATG 755

||||| |||||||||||||||||||||||||||||| |||||||||||||||||||||||

RaTART 569 TGCTCAAATATTTATCAACTGGATACAAATGGCTGCCAAAAAGGCAACTCCAGCTCCATG 510

Probe 1 756 TGACAAACCAGAATACGAAACCAATTACCCAACTGAAATTAGAGAGCTAATCAAGGAACG 815

||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||

RaTART 509 TGACAAACCAGAATACGAAACCAATTACCCAACTGAAATTAGAGAGCTAATCAAGGAACG 450

Probe 1 816 GCGCAAATTATGACGAATATGGCACGGATCCAGACATCCTTCAGATAAAAGGAACTTCAA 875

|||||||||| ||||||||||||||||||||||||||||||||||||||| |||||||||

RaTART 449 GCGCAAATTAAGACGAATATGGCACGGATCCAGACATCCTTCAGATAAAAAGAACTTCAA 390

Probe 1 876 TGTGGTGAGCAATCGTGTTAACTGACTTATCAAAGAATTCAAGCAGAGCTGCATTAGTGA 935

|| ||||||||||||||||||| |||||||||||||||||||||||||||||||||||||

RaTART 389 TGAGGTGAGCAATCGTGTTAACCGACTTATCAAAGAATTCAAGCAGAGCTGCATTAGTGA 330

Probe 1 936 ATACCTCAACGGATTGGGACCAGGAGCCGAAAAAGAATATTCTCTATGGA-AAG-C--CA 991

|||||||||||||||||||||||||||||||||||||||||| ||||||| ||| | ||

RaTART 329 ATACCTCAACGGATTGGGACCAGGAGCCGAAAAAGAATATTCACTATGGACAAGACGACA 270

Probe 1 992 CAAGACGACTTAAAAGACCAACAGTCCAAGTGCCGCCAATCAAAATATGCACAAGGCCAA 1051

||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||

RaTART 269 CAAGACGACTTAAAAGACCAACAGTCCAAGTGCCGCCAATCAAAA-ATGCACAAGGCCAA 211

Probe 1 1052 TGGATTAGAAAGGATAAAGAAAAAGCTGAACTCTTTGCCCAGTATCTTTCGAGTGTATTT 1111

|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||

RaTART 210 TGGATTAGAAAGGATAAAGAAAAAGCTGAACTCTTTGCTCAGTATCTTTCGAGTGTATTT 151

Probe 1 1112 CAACCACATGAAATAAACTCGAACATCAGCACGGCTCCAGAATACCT 1158

|||||||||||||||||||||||||||||||||||||||||||||||

RaTART 150 CAACCACATGAAATAAACTCGAACATCAGCACGGCTCCAGAATACCT 104

Score = 110 bits (59), Expect = 2e-20

Identities = 66/69 (95%), Gaps = 1/69 (1%)

Strand=Plus/Minus

Probe 1 1 GATATTGCACTCATTTCGGAGACGCATTTCTCGAACAAGAACTTCATTAAATTCAAAGGC 60

||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||

RaTART 1203 GATATTGCACTCATTTCGGAGACGCATTTCTCGAACAAGAACTTCATTAAATTTAAAGGC 1144

Probe 1 61 TACTGCACC 69

|| |||||

RaTART 1143 -ACGGCACC 1136