Research Plan Sample 1

Rationale – I have decided to conduct research and complete a project on the processes of acid erosion on human teeth. As a society that constantly consumes soda, I would like to discover the most effective common used type of toothpaste to prevent acid erosion.

Hypothesis – I think that by brushing the teeth with cavity protection toothpaste, that this will prevent less erosion than another type of toothpaste.

Procedure –

  1. Gather all materials. (Teeth are being obtained from Dr. Maykovich, DMD located in Carrolltown, Pennsylvania. Teeth were sterilized via autoclaving by dentist’s office before researcher picks up teeth.)
  2. Mass the teeth individually and record the mass.
  3. Photograph each tooth and record the color.
  4. Label each tooth A-E
  5. Brush tooth A with 0.5 g of Colgate Max Fresh
  6. Brush tooth B with 0.5 g of Colgate Baking Soda and Peroxide toothpaste.
  7. Brush tooth C with 0.5 g of Colgate Whitening toothpaste.
  8. Brush tooth D with 0.5 g of Colgate Cavity Protection
  9. Leave tooth 5 unbrushed.
  10. Fill 4 cups with 118 milliliters of Pepsi.
  11. Allow the teeth to soak in the Pepsi for 12 hours.
  12. I will record color and mass of each tooth every 12 hours.
  13. I will repeat this process for a period of seven days.
  14. Analyze data
  15. Report results.

Risk/Benefits – What steps or procedures you coulduse to prevent tooth decay

Data Analysis: I will find the mass of the teeth and analyze the data with the average.

Bibliography -

Research Plan Sample 2

Rationale: To see how different liquids (acids) decompose bones differently. It could help preserve bones in the real world. The reason I am doing this project is because I am interested in the decomposition of bones.

Hypothesis: Citric acid, lactic acid, and acetic acid will decompose bones at different speeds. Vinegar will decompose the bones the fastest because it has the highest acidity out of all of the liquids used. Milk will decompose the bones the least because it is the closest to neutral and has the least amount of acid.

Procedures:

1.Gather all materials needed.

  • Orange Juice
  • Vinegar
  • Water
  • Milk
  • 400 mL beakers
  • Cleaned Chicken Bones (bones saved after boiling bone-in chicken and removing the meat and washed with antibacterial soap & water after meat had been removed.)

2.Fill three different containers with...

  • Orange Juice (citric acid)
  • Vinegar (acetic acid)
  • Water (to have a healthy bone to compare with the others)
  • Milk (lactic acid)

3. Place one bone (each from the same animal and of the same thickness and size) into each sample of liquid.

4. Take notes every day for a certain period of time.

  • observe the size
  • the thickness
  • how bendy it is
  • see if the bone is losing mass

5. Analyze the data by recording what bones decompose the fastest and the slowest.

Risk and Safety: Be careful not to get any liquids in your eyes. To prevent contact be sure to wear goggles.

Data Analysis: I will use graphs and charts to represent the data collected and find the average mass after the experiment is over.

Bibliography:

  • education.seattlepi.com/science-projects-bone-decay-4862.html

Research Plan Sample 3

Rationale: Research has shown that using hand sanitizer is not effective after three uses. Does the effectiveness of hand sanitizer work after every trial?

Hypothesis: I believe if hand sanitizer is used so many times it won’t be killing anymore cultures.

Procedures:

  1. Gather all materials. (Petri dishes, prepared nutrient agar, latex gloves, marker, sterile cotton swabs, brown paper bags.)
  2. Prepare nutrient agar according by melting prepared agar in a beaker of boiling water.
  3. Prepare 8 petri dishes with nutrient agar.
  4. Label the petri dishes in the following titles:
  5. Left Hand 8:00 AM
  6. Right Hand 8:00 AM
  7. Left Hand 10:48 AM
  8. Right Hand 10:48 AM
  9. Left Hand 12:10 PM
  10. Right Hand 12:10 PM
  11. Left Hand 1:26 PM
  12. Right Hand 1:26 PM
  13. Left Hand 2:50 PM
  14. Right Hand 2:50 PM
  15. The following morning, label 1 latex glove “left hand” & label 1 latex glove “right hand.”
  16. Put gloves on appropriate hands beginning at 8:00 AM.
  17. Swab the left hand glove by rolling a clean cotton swab on the glove and swiping onto the gelled agar in the petri dish labeled with the appropriate hand and time.
  18. Repeat steps 7 with the right hand.
  19. Continue wearing gloves throughout the day, removing only before using the restroom or eating. Place gloves into labeled brown paper bags while using the restroom and eating.
  20. Swab each hand by using the procedure in step 7-8 at the times mentioned in step 4 labeled on the petri dishes.
  21. Incubate petri dishes at 37 degrees Celsius.
  22. Count the growing colonies daily and record data for 7 days.
  23. Analyze data.

Risk and Safety: Possible risk is someone could get hand sanitizer in their eyes or in their mouth. Student will prepare agar under supervision of qualified scientist. Petri Dishes need to be kept away from students and in an area that will not be disturbed. Petri Dishes will be incubated in qualified scientist back storage room, which is off limits to all individuals except the researcher and qualified scientist. Caution will be taken to not open the petri dishes once they are swabbed. Petri Dishes will be heated to kill any bacteria before disposal at the conclusion of data collection.

Data Analysis: Colonies will be observed and counted daily. Appropriate statistical analysis will be used to further investigate the data.

Bibliography:

Potentially hazardous biological agents research:

This researcher worked with the qualified scientist to determine that bacteria is classified as BSL-1. Bacteria, if any, will be collected from the transfer of bacteria from floor to tested item. The most common types of household bacteria are: Micrococcus, Staphylococcus, Bacillus, and Pseudomonas. Petri dishes will remain in qualified scientists storage room at all times. The researcher and qualified scientist will be the only individuals with access to this room. Petri dishes will remain closed immediately after swabbing. Petri dishes will be autoclaved before disposal to kill any remaining bacteria.