Laboratory Safety And

Chemical Hygiene Plan

revised 12/07

Laboratory Safety and

Chemical Hygiene Plan

Principal Investigator______Date filed______

Department ______Building/Floor/Room ______

E-mail address ______Phone ______

Fax number______

Laboratory Manager/RA______Phone ______

Departmental Safety Officer______Phone ______

Fax number: ______

********************************************************************************************

Please complete and forward a copy to the Safety Office

Keep a copy in the laboratory.

The Principal Investigator and/or the Laboratory Managerhave personally discussedthe hazards and the proper procedures for using and storing hazardous substances with allpersonnel who are or will potentially be exposed to such hazards.

The Principle Investigator has reviewed this plan and certifies that it reflects the current condition of his/her laboratory.

Principal Investigator’s Signature: ______Date: ______

Chemical Hygiene Plan

revised 12/07

List of Laboratory Personnel (MUST BE UPDATED ANNUALLY)

Department: ______Building: ______

Floor/Room: ______Extension: ______Training (dates)

NAME Read Plan Bloodborne Lab Safety shipping biological; (Signature & date) Pathogen dangerous goods, dry ice

P.I. ______

1 ______

2 ______

3 ______

4 ______

5 ______

6 ______

7 ______

8 ______

9 ______

10 ______

(Each lab worker, including P.I., must provide this information)

Chemical Hygiene Plan

revised 12/07

LOCATION OF THE NEAREST SAFETY EQUIPMENT

Department: ______Building/Floor/Room: ______Extension: ______

University Police ______Occupational Health ______

Safety Office ______

Eye Wash Station ______

Safety Shower ______

Fire Blanket (if chemical fume hood present) ______

Fire Extinguisher ______

Spill Clean-up Kits:

Acids ______

Alkalis ______

Organic Solvents______

Personal Protective Equipment (PPE)

Safety eye wear (glasses/goggles) ______

Gloves ______

Aprons/Laboratory Coats______

Face Shields ______

Mask/Respirator______

Laboratory Safety and Chemical Hygiene

Plan______

Reference Chemical/Radiation Safety Manual ______

Designated meeting area outside for a head-count after evacuation?

______

POST a copy of this page in a conspicuous place in the LABORATORY

Training:

Bloodborne Pathogen training:Is a requirement OSHA 29CFR 1910.1030 and is required every year if your lab works with any human blood, body products or cell lines. Training must be done annually and records kept for 3 years.

Laboratory Safety Training: This training is required annually. This training covers Chemical and Biological Safety Training. Guidelines from BMBL 5th edition and OSHA 1910.1450.

Shipping Dangerous Goods: Shippers are directly responsible for the correct and legal transport of dangerous or hazardous materials by ground or air. Anyone involved with shipping hazardous substances such as chemicals, radioactive materials or biological materials, such as diagnostic specimens, genetically modified micro-organisms, infectious agents, or Select Agents, is required to receive training. International Air Transport Association (IATA) Dangerous Goods regulations Training can be completed by using SAF-T-PAK Shipping CD. To obtain the SAF-T-PAK Shipping CD, contact the Safety Office 55410. The training is good for two (2) years.

Chemical Inventory Form

An inventory of all hazardous substances is required to comply with the State of Louisiana Right to Know Law. A hazardous substance, according toRight to Know Law, is defined as one that presents a physicalor health hazard and is listed in the hazardous substance list. Chemicals that exhibit theproperties of flammability, corrosivity, reactivity, or toxicity, are examples of hazardoussubstances that must be listed in your inventory.

Complete Chemical List is kept in: ______

(location in the laboratory)

In addition, include known and suspectedcarcinogens, mutagens, and teratogens that may not be listed in the hazardous substance list.

For a complete list of hazardous substances that must be included in your inventory, please refer to the Chemical Safety Manual or call the Safety Office 55410.

The inventory must include:

1. The proper chemical name or active ingredient (if material is a commercial product).

2. The Chemical Abstract Service Number (CAS#). If the chemical is a mixture, as opposed toa pure substance, the CAS# for each hazardous substance in the

mixture must be included.

3. The type of hazard, flammable, corrosive, carcinogen, toxin, irritant,sensitizer, hepatotoxins, nephrotoxin, and neurotoxin.

4. The physical state (solid, liquid, or gas) of the hazardous substance or mixture.

5. Average quantity, in pounds, of the hazardous substance used or stored.

6. Container type (glass, plastic, metal) in which your hazardous substance is stored.

7. Manufacturer name and catalog number, if available. (Optional)

The Laboratory Chemical Inventory must be complete, updated and a copy

submitted to the Safety Office on an annual basis.

In addition, Chemicals must be properly labeled with the chemical name and a copy of the MSDS* must be available in the laboratory. A Standard Operating Procedure (SOP) for each Hazardous Chemical must be written and on file for laboratory personnel.

*Material Safety Data Sheets - Material Safety Data Sheets (MSDS’s) must be accessible toall laboratory staff and visitors. They are available from the chemical manufacturer or through the Safety Office.

The laboratories’ Material Safety Data Sheets (MSDSs) are located:

______

(must be readily accessible to any laboratory workers)

Chemical Hygiene Plan

revised 12/07

Evacuation Plan for Laboratory

LSUHSC OCCUPANT EMERGENCY ORGANIZATION FOR LAB No: ________

Occupant Floor Manager (BRI): ______Phone #_____

Principal Investigator: ______Phone #_____

Laboratory Manager: ______Phone #_____

List below personnel in your lab that will be responsible for the specific duties in case of a total evacuation of your laboratory:

CallUniversity Police(56165):______

Call 9-911: ______

Call Safety Office (55410):______

Turn off all ignitable sources: ______

Close Fume/Biological Hood Sashes: ______

Notify others on floor: ______

Pull Fire Alarm: ______

Close Doors: ______

Get Head Count at Evacuation meeting place: ______

Designated Evacuation Meeting Place: ______

(For help in determining a designated meeting place,look for a map of the parking lots under Security and Parking on the Web-site. Questions please call the Safety Office.)

POST IN LABORATORY

Chemical Hygiene Plan

revised 12/07

Laboratory Equipment

1. Compressed Gas Cylinders

2. Ultraviolet (UV) Light Sources

3. Centrifuges

4. Sub-zero freezers

5. Biological Hoods

6. Intense Visible Light Sources (including LASERS)

1. Compressed Gas Cylinders. Indicate name of gas, size, and room location.

a. Cylinders must be properly secured.

2. Ultraviolet Light Sources. The sources listed below emit light with wavelengths in the range from 185-290 nm. The design, use, and maintenance of these sources must meet current standards.

a. Proper PPE’s must be used and available to all laboratory personnel.

3. Centrifuges. Indicate location

4. Sub-zero freezers. Indicate number of freezers and location

5. Biological Hoods. Indicate number of hoods and location. Training for use and cleaning of biological hood.

6. Intense Visible Light Sources (including LASERS).

Please contact Karen Kafai in the Safety Office 55410.

REGISTRATION DOCUMENT FOR THE USE OF LASERS

Principle Investigator MUST complete when proposed research involves use of laser (class 3b or 4). A separate registration form must be submitted for each piece of equipment in this category. Call the Safety Office at 675-5410 if you have any

questions or need any assistance. (PLEASE TYPE.)

PI NAME: ______DEPARTMENT:______

BLDG/ROOM: ______OFFICE PHONE: ______EMERGENCY PHONE: ______

LASER SYSTEM LOCATION: BLDG______FL/ROOM #______

USER’S NAME ______PHONE # ______

Are safety signs posted on door? Yes No

Are safety glasses used? Yes No

Are written SOPs* available? Yes No

Are users trained on the SOPs? Yes No

LASER DESCRIPTION: PLEASE DESCRIBE SPECIFICATIONS/CHARACTERISTICS OF THIS EQUIPMENT:

1. Type: ______

2. Manufacturer: ______

3. MODEL NO: ______4. SERIAL NO: ______

5. LASER CLASS: CLASS 3A CLASS 3B CLASS 4

6. TYPES OF OPERATION: (A) C.W. ______(B) PULSED ______

7. RATED POWER OR ENERGY OUTPUT: ______8. PULSE REPETITION FREQUENCY: ______

9. OPERATING WAVELENGTHS: ______10. BEAM DIAMETER: ______

11. MAXIMUM EXPECTED EXPOSURE DURATION PER DAY______

12. OTHER PERTINENT INFORMATION: ______

______

13. IS SERVICE FOR LASER DONE IN HOUSE? YES NOCONTRACTED OUT? YES NO

IF CONTRACTED OUT, COMPANY______

14. DATE OF MOST RECENT SERVICE: ______/ ______/ ______

REGISTRANT'S SIGNATURE: ______DATE: ______

EH&S APPROVAL SIGNATURE: ______DATE: ______

SOPs - Standard Operating Procedures

For more information contact Karen Kafai at the Safety Office 5327.

General Rules for the Laboratory

In the lab you are responsible not only for your own safety but for others in the lab as well. Here are some general guidelines for lab safety.

-Be prepared for the lab procedure; go over the protocol before your start.

-Maintain a clean work area

-Read Material Safety Data Sheets for the chemicals you will be working with.

-Know where all of the safety equipment is located; fire extinguisher safety shower, spill control protocol, eyewash station, and fire exits.

-Do not taste chemicals

-Wear PPE”S; goggles, face shield, gloves, and apron or lab coat.

-Wear appropriate clothing;

Shoes are closed toed.
No shorts, legs should be covered.
No sandals or flip-flop are to be worn in the lab.

-Long hair should be tied back.

-Do not eat, drink, smoke, or apply cosmetics.

-Do not use flammable liquids near ignition sources.

-When you make a solution make sure you label your container, name of solution, date it was made (m/d/y/), concentrations, and your name or initials.

-Follow proper instructions for disposal of all chemicals. Waste chemicals should not be mixed. Contact the Safety Office if there are any questions 55410.

Ask your supervisor how to dispose of the chemicals.
Contact the Safety Office for chemical pick up 55410

-Report all chemical spills and accidents to your supervisor immediately.

Contact the Safety Office 55410

-Clean your area when you have completed your work.

-Dispose of all biohazard waste in appropriate container.

Call Environmental Services: House keeping to obtain biohazard boxes 5-6337.

-Dispose of all sharps in appropriate container.

Can obtain through Central Medical Supply(CMS) 5-7050
Or order through a Vendor

-Do not wear gloves outside if the lab, always remove gloves before leaving the lab.

-Do not pipette by mouth.

-Date all chemicals; date received, date opened, date expires.

-No minors allowed in the labs

Fire Safety and Emergency Response to Fires and Explosions

Research laboratories differ from other work environments in that they usually contain a variety of fire hazards. In addition to the 'ordinary' (Class A) fires, those fueled by wood, paper, textiles, etc., laboratory hazards include the presence of flammable and, quite often, volatile solvents such as petroleum distillates which are not miscible with water, reactive metals such as sodium and potassium, flammable metal powders such as magnesium, titanium, and zirconium, metal hydrides such as lithium hydride, lithium aluminum hydride, and sodium borohydride, as well as many kinds of electrical equipment.

Complications arise when fighting these fires because each type of fire must be fought with the extinguishing agent and procedure (Read your MSDS’s for more information), appropriate for it; the use of the wrong technique or extinguisher can be catastrophic. The Safety Office has simplified fire-fighting in the laboratories by equipping all laboratories with multi-purpose (ABC) dry chemical fire extinguishers, which can be used on all types of fire with the exception of reactive flammable metals which must use extinguishers suitable for the particular metal. Fire extinguishers are inspected every month by the Safety Office. However, laboratory personnel are responsible for seeing that extinguishers are promptly recharged after each use; call the Safety Office (5-5410) for pick-up and replacement.

Before acting to cope with a particular fire, the fire must first be judged as being controllable by laboratory personnel. Since this depends on the rapid, yet careful, evaluation of several factors, the judgment of the person making the decision is critical.

The size of the fire.
Its intensity.
The nature of the burning substance.
How near are other flammable or explosive materials to the fire, whether fighting the fire would place the laboratory worker doing so in substantial risk?
Availability of escape routes.

Prospect of the fire spreading and availability of the proper fire-fighting equipment, are factors that must be considered in making this decision.

Should the nature of the fire, its size, etc. make it controllable, use the appropriate available fire extinguisher as described below, and proceed with the methods described in the section on controllable fires. On the other hand, should the fire be judged uncontrollable, follow the evacuation and notification procedures for uncontrollable fires.

In all cases, however, call Security (ext. 5-6165) and the Safety Office (ext. 5-5410).

Classification of fires

Class A (Wood, paper, textiles, rubber, coal)

This type of fire can be extinguished by the ABC extinguisher.

Class B (Flammable or combustible liquids, greases, petroleum products, solvents)

Carbon dioxide or dry chemical ABC extinguishers should be used. Carbon dioxide extinguishers do not leave any residue, whereas dry chemical devices do. Pressurized water units should not be used since the immiscibility of most solvents and water would result in the spreading of the fire.

Class C (Live electrical equipment involved in a fire)

If possible, turn off the electrical power to the devices, and then use either the dry chemical extinguisher or a carbon dioxide.

Class D (Sodium, potassium, magnesium, titanium, zirconium and other metals)

If sodium, potassium, magnesium, or any of the flammable metal powders are to be used in a laboratory, the appropriate dry powder extinguishing agent must be made available for fire emergency use before work is started.
Use specific "Class D" extinguishing agent or any of the following dry powders: graphite, limestone, sand, or sodium carbonate.

Fire-fighting procedures for controllable fires

Clothing fires must be extinguished immediately, before anything else is done, in order to minimize skin burns.
For all fires, the fire alarm must be activated to ensure the Fire Department response.
The decision of whether to fight the fire oneself or to wait for fire-fighting help must be made according to the type and size of the fire, its location and the circumstances of the fire. A small fire in a container may be easily snuffed out by the placement of a nonflammable cover across the container opening. A small fire in an area free of other fuels can be extinguished with appropriate available extinguishers before calling for help.
When extinguishing a burning solid, direct the extinguisher discharge at the base of the flame; in the case of burning liquids, direct it at the leading edge. Larger or rapidly growing fires are best left to the Fire Department.

Remember the word PASS to use a fire extinguisher properly:

Pull the pin out of the fire extinguisher.

AIM the fire extinguisher at the base of the fire or edge of the fire.

Squeeze the trigger.

SWEEP back and fourth over the fire to put it out.

In the case of an occurrence of any fire, the fire must be reported to University Police at ext. 5-6165. There are no exceptions.

Evacuation procedures for uncontrollable fires

Leave the area of danger. DO NOT stay to fight a large fire. On your way out, if it can be done safely, turn off equipment and move any explosive or flammable materials away from possible contact with hot surfaces or other sources of ignition. Your safe exit, however, must be given the highest priority.
Pull the fire alarm, notify personnel on the floor, and call Security (Ext 5-6165) to tell them exactly where the fire is located. Security will have notification of what floor, but not the lab number.
Leave by means of one of the predetermined evacuation routes for your laboratory area. Make sure to close the door as you leave.
If considerations of safety make it necessary to leave the building, evacuate promptly. Never use the elevator as an evacuation route. Always use the stairs.
All personnel on the floor will evacuate area by proceeding to nearest stairwell and exiting to ground level. Once outside move away from the building, to your departmental predetermined meeting place, not only for your own safety, but also you will not impede the Fire Department’s operations.
If a person's clothing is on fire, he/she must not be allowed to run, as this will fan the flames and cause a more serious burn. Either put the person under a shower or wrap him/her in a fire blanket, coat, or whatever is available to smother the flames. Roll the person on the floor if necessary. After calling the emergency numbers, place clean, wet, ice-packed cloths on small burned areas wrap the person warmly to avoid shock, and secure medical assistance, take the person to the Emergency Room, located on the first floor of the Hospital (5-6880).

Remember STOP DROP & ROLL

Each person in the lab must know the following:

Where is the nearest pull station?
Location of fire extinguisher.
Location of two remote exists.
If a fire occurs in your area the steps to follow:

Rescue

Contain

Alarm

Extinguish / Evacuate

Should you use elevators to evacuate?
Where is your designated meeting area outside for a head-count after evacuation

Chemical Hygiene Plan

revised 12/07