Fig. S1. Pictures showing the clearly different colony colours of strain NEAU-HZ10T and closely related Streptomyces strains on ISP 2, ISP 3, ISP 7 agar and DSMZ medium 65 at 28 oC for 14 days.

Fig. S2. Phospholipid compositions of strain NEAU-HZ10T.

a, Using ninhydrin reagent; b, Using molybdenum blue reagent; c, Using molybdophosphoric acid reagent;

Abbreviations: PE, phosphatidylethanolamine; PI, phosphatidylinositol; PL, unidentified phopholipid.

Fig. S3. Neighbour-joining tree based on 16S rRNA gene sequences showing relationship between strain NEAU-HZ10T and related taxa. The out-group used was Kitasatospora setae DSM 43861T (U93332). Only bootstrap values above 50 % (percentages of 1000 replications) are indicated. Bar 0.005 nucleotide substitutions per site.

Table S1. Cultural characteristics of strain NEAU-HZ10T.

Agar medium / Growth / The color of colony / Soluble pigment
Yeast extract-malt extract agar
(ISP 2) / Good / Dark Grayish Brown / Strong Brown
Oatmeal agar
(ISP 3) / Good / Grayish Brown / Light Grayish Brown
Inorganic salts-starch agar
(ISP 4) / Moderate / Strong Yellowish Brown / None
Glycerol-asparagine agar
(ISP 5) / Moderate / Light Yellowish Brown / None
Peptone-yeast extract iron agar
(ISP 6) / Moderate / Moderate Yellowish Brown / None
Tyrosine agar
(ISP 7) / Moderate / Light Brown / None
Glucose-yeast extract-malt extract
(DSMZ medium 65) / Good / Dark Grayish Brown / Strong Brown

Table S2. Comparison of cellular fatty acid contents (% of total) for strain NEAU-HZ10T and its closely related Streptomyces species grown in ISP 2 medium in shake flasks at 28 oC for 14 days.

Strains: 1, NEAU-HZ10T; 2, S. cocklensis DSM 42063T; 3, S. yeochonensis CGMCC 4.1882T; 4, S. paucisporeus CGMCC 4.2025T; 5, S. yanglinensis CGMCC 4.2023T. All data are from this study.

Fatty Acid / 1 / 2 / 3 / 4 / 5
Saturated
C14:0 / 0.6 / 1.5 / 0.8 / – / 1.2
C15:0 / 8.3 / 12.5 / 6.6 / 6.6 / 7.2
C16:0 / 12.2 / 18.4 / 17.9 / 18.6 / 19.8
C17:0 / 2.7 / 2.6 / 4.7 / 2.3 / –
C18:0 / 4.2 / 5.5 / 10 / 6.3 / 3.8
Unsaturated
C16:1ω7c / 1.9 / – / – / 5.1 / –
C16:1ω9c / – / 1.5 / – / – / –
C17:1ω7c / – / 1.4 / – / – / –
C18:1ω9c / – / – / 4.2 / – / –
Branched
iso-C14:0 / 9.2 / 5.7 / 8.7 / 4.9 / 7.8
iso-C16:0 / 37.1 / 19.0 / 18.2 / 23.3 / 24.0
iso-C17:0 / – / – / – / – / 1.4
anteiso-C15:0 / 13.3 / 25.3 / 19.3 / 24.1 / 23.8
anteiso-C17:0 / 6.7 / 6.8 / – / 8.9 / 5.6
cyclo C17:0 / 3.8 / – / 9.8 / – / 5.4

–, not detected