ELECTRONIC SUPPLEMENTARY MATERIAL

Supplementary Table 1. Statistics of GeoOAS1Aa and GeoOAS1Ab differences

Exon No / Length / Mutations
GeoOAS1Aa / GeoOAS1Ab / Point mutations
in codon position / Syn / Indels / Ts / Tv / All / All /
Length
(%)
1 / 2 / 3
1* / 23 / 23 / 1 / 0 / 0 / 0 / 0 / 1 / 0 / 1 / 4.3
2 / 68 / 68 / 0 / 1 / 5 / 5 / 0 / 5 / 1 / 6 / 8.8
3 / 133 / 133 / 3 / 4 / 1 / 1 / 0 / 6 / 2 / 8 / 6.0
4 / 250 / 247 / 2 / 4 / 6 / 4 / 1 / 8 / 4 / 13 / 5.2
5 / 58 / 58 / 1 / 3 / 0 / 0 / 0 / 4 / 0 / 4 / 6.9
6 / 137 / 137 / 1 / 1 / 3 / 3 / 0 / 4 / 1 / 5 / 3.6
7 / 51 / 51 / 2 / 0 / 1 / 1 / 0 / 3 / 0 / 3 / 5.9
8* / 198 / 198 / 2 / 1 / 2 / 2 / 0 / 3 / 2 / 5 / 2.5
Total / 918 / 915 / 12 / 14 / 18 / 16 / 1 / 34 / 10 / 45 / 4.9
Intron
No / Length / Mutations
GeoOAS1Aa / GeoOAS1Ab / Length** / Indels / Ts / Tv / All / All /
Length**
(%) / Ts+Tv /
Length**
(%)
1 / 50 / 42 / 43 / 1 / 0 / 0 / 1 / 2.3 / 0
2 / 178 / 174 / 158 / 7 / 10 / 2 / 19 / 12.0 / 7.6
3 / 66 / 67 / 67 / 1 / 1 / 1 / 3 / 4.5 / 3.0
4 / 55 / 62 / 53 / 2 / 1 / 3 / 6 / 11.3 / 7.5
5 / 53 / 51 / 52 / 1 / 2 / 3 / 6 / 11.5 / 9.6
6 / 59 / 60 / 60 / 1 / 3 / 2 / 6 / 10.0 / 8.3
7 / 186 / 187 / 187 / 1 / 2 / 0 / 3 / 1.6 / 1.1
Total / 647 / 643 / 620 / 14 / 19 / 11 / 44 / 7.1 / 4.8

* - statistics based on the partial primary structure

** - contiguous stretches of nucleotides missing in the other gene (according to the alignment in Fig.1) are treated as single indels and added to the number of aligned nucleotides

Ts, transitions; Tv, transversions


Supplementary Table 2. Specification of genes in two OAS loci in A. queenslandica

Locus A

Gene number / Orientation / Title / Method / EST / Protein homology
1 / + / Iqcd / EST initiated gene prediction / CABF10427 / Score 199 bits to XP_001636992 predicted protein [Nematostella vectensis], NEMVEDRAFT_v1g93943, containing IPR000048 IQ calmodulin-binding region
2 / - / Gats / EST / CAYI10006
CAYI10007
CABF7938
CABF13842
CAYI1437
CAYI3490
CAYI3848
CAYI8152 / Score 232 bits to XP_001636192 predicted protein [Nematostella vectensis], NEMVEDRAFT_v1g163533, 213 bits to NP_109644 opposite strand transcription unit to Stag3 (GATS) [Mus musculus]
3 / - / TYW1_
Radical_SAM+
Wytosine_form / EST initiated gene prediction / CABF20485
CAYI2711
/ Score 713 bits to XP_001188746 PREDICTED: similar to radical S-adenosyl methionine and flavodoxin
domains 1 [Strongylocentrotus purpuratus]
4 / + / AmpOAS1Aa / Gene prediction / - / Score 190 bits to CAC82933 putative (2-5)A-1 synthetase [Suberites domuncula]
5 / + / AmpOAS1Ab / Gene prediction / - / Score 190 bits to CAC82933 putative (2-5)A-1 synthetase [Suberites domuncula]
3 / - / TYW1_flavodoxin / CAYI5477
6 / - / Protein_117_aa / EST / CAYI3012 / No evident homology
7 / - / ORF_839_aa / ORF / - / Three non-contiguous hits (Score 76, 38 and 71 bits, respectively) to XP_001642127 predicted protein [Nematostella vectensis], NEMVEDRAFT_v1g237807, containing BTB/POZ domain in C-terminal region

>Iqcd

IGSYEESRVILYPSQKKLSSVESQRVMTVAKETRRKAEAALLLIPHLYDHLDRFAVTMGTELVARIREYHYLSEEYERCYESLIENEIEPDFATSRSKSSLPDHKLEPISSNMSPEELQIRFFSLQQQLRDLTRTLLRELQKCPSLDAIAKEVKGPVPPRVDNVIGQLGEVVRLMEETLLTTHEEEVKRNEYLSIISERRQVAETEIASLQSELQHARNDLNKEVQKREDHRKKLIHEIRVVDKLAKEVQKKIEDDTNKQEANSQKLHDGQVSKANEEIGILKKTLLEAKAANKEIESQLRKKSFKRETEADNWIQKYDNEIGDLQNELETLTEEFTEEKKQVKEIGERLEILSIEYDAVVKEQEEERKKAEEAQQRLASMVRAATQIQSCWRSYKIRKMFSKDAKKGGKKGKKGGKKGGKKGSAKKKKA

>Gats

MSVRPALHLLPLELRIASIGKDDLPSFTYPLLQMTLGLLGQQSSCRQNHFFSFTETKNDYSLILDNELFKELIQFPGSEALQVAPESWRPLTIEVGAFGSLTGISKLVASVIGPLADEGVSVFCLSTNQEDYVMVKEKDLYKAMTCLHPCFKLLAELEQDVQLIENFSHDNVTNQIKNDLNYIELPSPRSITHPFTCTASKFHVCSILPSTLPSIAQPLLQLMFYNTRSDDVFLSLSIISDDISMVLDARDIDKFPEDSVYSSEAYWKVITIGDGPLGFDECGIVAQVAAPLAQADISTYYICTFYNDHTLVPEGSVTKALQLLNKYLMSSKVDNSSSPVSVGSLNGLTHPPSQFRTEPLPRLPLTNPVA

>TYW1_Flafodoxin+Radical_SAM+wytosine_form

MATTPYLFVDVFLDISKEINLSLLFICFIVLLASFYWLYRRPSFPSKVKRTGPLSVRVLYASQTGMGKRFAEQLMSEIKSRGFHSSVSDVSHIDPDEEFTTPELSPCTLYVFLISTYSEGFPPDSARWVFQWLRESVDDCRVSKSLLSGLCFAVFGCGNSLYKDHYNTAAKNLFDWLVQLSAKSVCQLGLGDANVSQSEHGGLSNDFAAWKNQFLESLERLRNESDENRTKASDHNSKFYSNQNGNESRQDGSESLRGVSYEEDLDGSSTDDEGVGSEDDVVDVEDIGDMMVKMKKASADNKRQNEKKKREMVTPEIRQSLTKQGYRIIGSHSGVKLCRWTKSMLRGRGGCYKHSFYGIESHRCMETTPSLACANKCVFCWRHHTNPVGTEWVWPMDGPERIAQEALANHYQMIKEYRGVPGVRGERLAEGMKVKHCALSLVGEPIMYPEINKLIGLLHEKKISTFLVTNAQFPDAIRTLSPVTQLYVSVDASPKESLKKIDRPLFKDYWSRFIESLKALPDKGQRTVYRLTLVKQWNTDELEGYAELVSLGQPDFIEIKGVTYCGESKASSLTMANVPWHDEVLTFVKELADLLPDYEMACEHEHSNCVLLSHRKFFIDGKWHTWIDYDKFHELYNDTAGSKSFTALDYMAVTPSWSLIGSAERGFDPGETRWTRKSKRKAESGC

>AmpOAS1Aa

MEFPRDTCEDIPCKRNGVRQYLKEDTRLTPAEIIKGGSLGHGTAIPGDFDLDLVLYSRDIDPEVVLEIGVDNILDRLDVFLRRRLPEAYVHVKKTSFSLQFKLNGVIDVDLLPSPYWGEDPDQFHRFLEEKGEKERMNFSCSAAKWQVKFFKEQPNEVKEYIKRFKAWRNLHWPKGQDAVGRPKSYLLGLLMIRAYEKSKDKSIHIKWNDGYYDWTYRLTREEFQSLLGNRFTLISSSNGVDVVYDNKQQRQRNYYPQKVPSILDVANPFNDVYVSGIGNYHCKDPQGSFHPGTGKWSPLVGKIDTFSLSKPLEYYNN

>AmpOAS1Ab

MNKLTKNSQGHEDFPCKRKQYLKEDTRLTPAEIIKGGSLGHGTAIPGDFDLDLVLYSRDIDPEVVLEIGVDNILDRLDVFLRRRLPEAYVYVNKTSFSLQFKLNGVIDVDLLPSPYWEDPDPFHRFLEGKDKKDKMNFSCSAAKWQVKFFKEQPNEVKEYIKRFKAWRNLHWPKGQDAVGRPKSYLLGLLMIKAYEKSKDKSIHIKWNDGYYDWTYRLTHEEFQSLLGNRFTLISSSNGVDVVYDKKQQRERTYTPAEVPSILDVANPFNDVYVSGIGNYHCKDPQGSFHPGTGKWSPLVGKIDTFSLSKPLEYY

>Protein_117_aa

MNNTTNGDFLQDVESFLYTPLGLGALAGATALLTSCLWLICCSVACCCIKWRNKRRESGTIEAGADLKYMTCTEFRPPHYSSATSTENGYHSNAGTLSSLNRHDALAIHSSMDAILN

>ORF_839_aa

NINGKAVLSRAGGIELLTQCLAEQENKGEKEKGQSSLIIDALCHCCRDVHGRQKVRECGALQVLINLLQSKDHSSFHHDILSALICYYFDEQTLQFMIRRLGLIKALLYHLKKGIKEEKDEGRREEREHNEEGEVRKMRKRKKRDQKGGRLVKRLRQQDDPDIFLKNEESFGSVEKSPGSSTKGSEDDINIDEGSGVDIEGDSLDEASDCNEEEHPIVKKSKRKKNEAKKKLSDQEKPSNSNNDLPTGTYHKDTEPPSKKLSRTISDEESTSSLYYLSPPYNMEDSGGGRGSFSPTRETDLAHSPLFEKLSPLLNINEDEASSYSATSPLSKGTFSPSCQAALEANNTLSSPVPTNFIDSLLSPTSCSPVAPSKTIKRSSSHEIGSNHSSSPHSKVLLLLSRVSHLHDCQPILASVDVLSVIMDYYLTMNLKDGHCFKVLSRLFSNPHCFQDCLVTLAPSMCFHHMIMTDDNGGVTNAQCNATSYDRRIATSNTQSTSYQRAMGYPSPPIGDLSHSICHKMLTEYGGLHNLLCLSKGQLLTTQCSCSQNTSTIEDTELKELTPSPPLPSSKVSDQETLTQLMAIDTLLYLGSQYRKCPSRKRRHSAIERRSSATLPLPIKAGTISPPPLSSPLCLYRDFDHWPFDTCIILDDGTMFPVHRRVLVESSEVFSVMLSGCYKESTDSSVSLHSVCPSSFRSLVHHAYGCGSHCWGRVSEKEAIDEHYDDEVIDEIVASIKEKEGQERGKHLLRVLISANQFFMCSLLTAAQESFMLYLSPKNVTPLFLFSQLHQCSLLSDATVQLLVGRGPGFCQSESFLRLVRSIECDEFLSTITTVFHQK

Locus B

Gene number / Orienta-tion / Title / Method / EST / Protein homology
1 / + / PDHE1alpha / EST / CAYI10060
CAYI3314
CAYI5926
CAYI10023
CAYI5668
CAYI2776
CABF10208
CABF8701 / Score 495 bits to XP_001175746 PREDICTED: similar to Probable pyruvate dehydrogenase E1 component alpha subunit, mitochondrial precursor (PDHE1-A) [Strongylocentrotus purpuratus]
2 / + / ORF_714_aa_
Ank_rep / EST and ORF / CAYH3434 / Score 134 bits to XP_420641 PREDICTED: similar to ankyrin B (440 kDa) [Gallus gallus]
3 / - / DUSL / Gene prediction / - / Score 400 bits to XP_00169015 predicted protein [Nematostella vectensis], NEMVEDRAFT_v1g199771, containing Dihydrouridine synthase-like (DUS-like) FMN-binding domain. 371 bits to XP_001231915 DUS4L [Gallus gallus]
4 / + / Protein_84_aa / EST / CABF15249
CABF11028
CABF16022
CABF26945
CABF11133
CABF19932
CABF22767
CABF10631
CABF17790
CABF26711
CABF6815 / Score 48.9 bits to ABK26429 unknown protein [Picea sitchensis], 40 bits to NP_565726 expressed unknown protein [Arabidopsis thaliana]
5 / - / ORF_707_aa_
Ank_rep / ORF / - / Score 151 bits to XP_001184164 PREDICTED: similar to ankyrin 2,3/unc44 [Strongylocentrotus purpuratus]
6 / + / ORF_702_aa_
Ank_rep / ORFs / - / Several ORFs with ankyrin-related matches, the longest ORF - 702 aa, Score 251 bits to XP_001649474 ankyrin 2,3/unc44 [Aedes aegypti]
7 / + / Acat2 / Gene prediction / - / Score 537 bits to XP_001630395 predicted protein [Nematostella vectensis] NEMVEDRAFT_v1g169195, acetyl-CoA acetyltransferase; PRK07491
8 / - / PITRM1 / EST initiated gene prediction / CABF23656 / Score 838 bits to XP_001638709 predicted protein [Nematostella vectensis], COG1026 Predicted Zn-dependent peptidases
9 / + / AmpOAS1B
(variants) / Gene prediction / - / Score 140-141 bits to CAC82934 putative (2-5)A-2 synthetase [Suberites domuncula]
10 / - / ORF_678_aa / ORF / - / CDD hit 38.8 bits to smart00256, FBOX, A Receptor for Ubiquitination Targets (PssmId 47585)
11 / - / PITRM1_
truncated / EST initiated gene prediction / CAYI2358
CABF1823
CABF15589 / Score 197 bits to XP_001181181 PREDICTED: similar to Pitrilysin metalloproteinase 1 [Strongylocentrotus
purpuratus] (stop codon in exon 6 leave protease domain incomplete; 3'-terminal parts of ESTs are PITRM1 non-related
12 / + / AmpOAS1C
(variants) / Gene prediction / - / Score 159-162 bits to CAC82934 putative (2-5)A-2 synthetase [Suberites domuncula]
13 / + / Aldo/ketolase 2 / Gene prediction / - / Score 306 bits to XP_001608172 PREDICTED: similar to CG18547-PA [Nasonia vitripennis], Aldo/keto reductase superfamily member
14 / - / Aldo/ketolase 1 / EST initiated gene prediction / CAYI9950 / Score 301 bits to XP_001195433 REDICTED: similar to ENSANGP00000008370 [Strongylocentrotus purpuratus], Aldo/keto reductase superfamily member
15 / + / Coiled-coil domain ATPase / EST initiated gene prediction / CAYH6884
CAYH1726
CAYH4066 / Score 105 bits to EDL30030 coiled-coil domain containing 21, isoform CRA_b [Mus musculus], containing conserved domain Smc - Chromosome segregation ATPases [Cell division and chromosome partitioning]

>PDHE1alpha

MAALIRGVRNLLPRGLVVARCASSSAEFSIPEYKLHKLDSGPSTTTTITRDELMKLFRDMTVIREMETEAKNLYLKKIIKGFCHLYIGQEACAVGMEASITKDDPVITAYRAHGWTYTRGIEPLGILAELTGKKSGCAKGKGGSMHMYAPNFYGGNGIVGAQVPLGAGIAFELKYNNKDNICITLYGDGAANQGQVFEAYNMAALWKLPVIFACENNRYGMGTAVHRSSASTDYYTRGDYIPGVYVDGMDVLAVREATRWAKEYILAGNGPLVMELDTYRYYGHSMSDPGKSYRKTEEVQQFRKEKDPITTATRYLIQGDLATEEELKEIKKSVQADIKKAVANAISDTELPLEEMYTDIYTSTPEFMVRGCDPFTWGKSERA

>ORF_714_aa_Ank_rep

IATPPLPLSMSVSGSSSPVLSVSLLLSKLLPVVDWEVLGLHLNIPKHELEKIRRQFFHEGVERCKAEMLDLWLKTSNEEALHWNVISDALTKMRGLEELAQQIRTMGPSQEERMSQEEATLSSEESPVTKVEFQFKKLDLKLFSKLESKFAKLVSDILQELKSRGVSVNELHSYVKIRLELKSSSSPQSLDELLLDKLKPYYCLTNLTLIDNIICEFLDGSKIQENLEEYEKQLESFKTSAKMEELVDHMTSSLEGKGEEGTVSVVLKLEGSWLGVTLKHFQQLVEEIFLERSCCFNHIQVKRGCICVSMIVPEAMTSSLVSLAKQRIEFINTIGIIRLAIGGNEIMDKEEEKDEASFSQYLMRGVLQEKIDVVRFILSLDGLGLADTISPCIEEAVESGNIQILSMLLESGGDPNFIGNKGLTPLQTASKQGHVTIVNKLLQYKADPNQDGVSGWTALMIATEKNNTSIIELLIKAGANLNAKMVHGWTPLMLACAYNFPALVSILLKAGADPNIRTIKWAAVHSAASKYEDPSILIDLAESGVNLNAVTGAGATPLILACQVNFELTINVLLNYKVSLDSQTRSGYTALMAAVCNKSVSIVESLLSAGANVHIADANGQTALDWALDSGHPEITELLLVYMGDMPATPLKAPSSAPSVTLVPEDIEVHIPSSSSAILELRDALENPLPPQGTSLKHKSSKEEKEKSRNTTAY

>DUSL

MAEREEENAKNIMDLFQPGAVVRMCAPMVRYSKLPFRKLVRRYGTQVAFTPMIVSESFVRSQKSRDVEFTTDPLDDRPLVAQFAANNVEDFSRASQLVSPFVDAVDLNCGCPQRWAMEEGYGSYLITRPELVCDMVRGAKRVAGIPVSIKIRIHEDLRETVELARRCEVIGLSWLTVHGRTVKQRAEPVNLSAIKLIKESVHIPVIANGDVRNESDINRTVQETGVDGVMAARGMLQNPAMFGGYDETPIECLEDWVDISLRSGLTFTSFHHHCMYMLERVQAKTERRVFNTLNSTPAVLEFLKHHYNIQHK

>Protein_84_aa

MASQKWISRPEKPPEKWATIDRSNAIEAWGRMRETTNQHFKFTPRTTAYCVIWALLVPIGIWKIIKWERQYKDRAKGRPPKDLF

>ORF_707_aa_Ank_rep

SYKRMSEVTKVSLPLLLSKLLPVVDWEVLGLHLNIPKHELEKIRRQFFHEGVERCKAEMFDLWLKTSNEEALHWNVISDALTKMRGLEELAQQIRTMNPSQEEMKDRVDSVASKSIQQATLVNLSVKVQKPCLKRFSQLESKFAKLVSDILQELKSRHVSVNEFHSYVKIRLELKLSSSPQSLDELLLDRLKPYYCLTNLTLIDNIICEFLDGSKVQENLEEYEKQLESFKTSTKMEELVDHMTSSLEGEGEEGTVSVVLKLEGSWLGVTLKHFQQLVEEIFLERSCCFNHIQVKRGCICVSMIVPEAMISSLVSLAKQRIEFINSIGIIRLAIGGNEIINKEEEKDEASFSQYLMRGVLQEKIDVVHFMLSLGGLGLADTISPCIEEAVESGNIQILSMLLESGGDPNFIGNKGLTPLQRASKQGHVPIVNKLLQYKADPNQDGIDGATALMLASNFDHFGIVEQLLSSGASVNKTMFDGWTALMFACDQNHLQIVKRLLQSGANPNVLTHSSKCSPVYFASSKNNNPSMLLTLIETGADINIATETGVTPLIIAAQFGFEAIVSVLLSHKALLDCQTHHGFTALMAAAHKGHVDITESLLLAGASTHIADNNGRTALEWSLQSEQHEITQLLLMNVGDVIDESDHAYNAEYQYGGKRISEDISSPKLHPSIIKLREAMRKPPLSEDTGQKHRHAIRWNNEHLVKK

>Acat2

MSLERGVFIIGAARTPVGSLNGSLSSLSAHQLGSTAITAVLERAKVLPEKVSEVLVGQILTAAQGQNPARQAAMLSGIPKEVPSTSINMLCGSGLRTIAMGYQSILVGDADIVVAAGQESMSQAPHAVHLRSGVKFGDASLTDTMMKDGLTDAFHNYHMGQTAENVAKQYGISREEQDRFAVSSQNKTEESQKNGVFKDEIVSVEIKTRKGPVTVDTDEFPRHSTTIEGLAKLKPAFVKDGSGTVTAGNTSGINDGAAAVVLASKEAIDGLSCSLPLARIVSWAQCGVDPQIMGMGPVPAVSKALQKAGWNKDDVDLFELNEAFAAQSLAVVKELNIDEKKVNVGGGAIALGHPIGASGCRILVTLLYALKRVGGKRGVASLCVGGGMGIALCIEMC

>PITRM1

MAAQLFSAPRLLSRRRRLIHLNNTVFNSTRREDVSKLSVAINDRISGYRVTGVTEVSDFNLKAIELLHESTKAQHLHLSRPESNNVFGVGFRTTPMDSTGISHILEHTTLCGSAHYPVRDPFFKMLTRPLATFMNAFTANDWTFYPFSTQNYNDYRNLLSVYCDCVFHPNLKKMDFHQEGWRLEHEDPNDRNTPLVFKGVVFNEMKGMFSNAEYLFDTELQRKLLPSHTYSHVSGGAPLNILDLTWDSLKEFHAKHYHPSNSYFYTYGDIPLSDHLSFIDENVLSKFSVSTPAAAIPLEARWNQPRSHEIYCAPDPMAPDPNKQTTVGVAYLLGPITDSYEGLLLSILTKLLIDGAASPFYQSLIDSNIGSDYSPSTGYNDGTKDSYFSVGLQGISTEDAEKVKQIITETFHKVYQEGFPEERVKAILHQVEIGLKHQSSNFGLALLMHFITPWIHGASPTSLLQINQNVENFKDHMANDPNYLKEKIKEYFIDNKHNLTLVEYNKKQKELEEEKLRLLVSSLSEDDKQRVYDEGLQLQEDQNKHQTSDCLPSLSVADVDKTTPITELIHEDHGSIPIQYCVQPTNGISYLQFLSSCRDLPDDLKPYLPLFCSVITKMGTQDLDYRQLSQQIDLYTGGSVCVGTHVASHHSIPNQFEQTFSTSNKLPQLFQVLSLSLILCSRPQLQDEQRLQTLIAMEASSLAVNVSRSGHRYAMTASASSLSPAAMMAEKYGGISQVLIHRSIIAFNVTEPQFTVASEAAEGFLDNLPGSITDTPLFMEEGTFTPTLSHTHYQLPFQVYYVGQSYRTVPYTHEDSPSLRVLAQLMGWKYLHKEIRYSLVVSCFVLNKQFLEGDPHSLDTVNRFNDSIDWVTSRSFTDEDVEEAKLSVFSQ

>AmpOAS1Ba

MAASQLMASCVSLLRHVVLFIVIENVLAMQPFRAHLHKDAGIVVSEIIQGGSLGQGTCVPMKFDVDLVIFSKSITHEMISNPDKRKEILTKVQQCLIRRLKRNYVDDKMTQNSVQFKYKHSPGHIIDVDVLTSPMWKSAGELSIYIAEHCPDQYYHLSAGASKWQTDFIRHQMPRVKEYIKRAKYWKKSIDWSPGREPSSYLMSLLVIKASDLVGARHYFYICSVSWKIKELVKNHHTIDIQFQTEYMFTVEAPSQPRVIDPANVCNNLYETGFGKQGKRDEPQDFWNVFADKIEGLDLSIPIERLGPLP

>AmpOAS1Bb

MAASQLMASCVSLLRYFVLFIVIENVLAMQPFRAHLHKDAGIVVSEIIEGGSLGQGTCVPMKFDVDLVIFSRSITHEMISNPDERKEILTKVQQCLIRRLKGNYVDDKMTQNSVQFKYKHSPGHTIDVDVLTSPMWESAGELSIYISEHCPDQYYHLSAGASKWQTDFIRHQMPRVKEYIKRAKYWKKSIDWSPGREPSSYLMSLLVIKASDLVGEHLLACMEHYYCFALCSISEKIKELVKSHHAIEIQFQTQYMFTTEAPAQPRVIDPANVCNNLYETGFGKQGRRDEPGDYWDVFAKKIESLDLSIPFERLGPLP

>ORF_678_aa

KHVYSRVSGHRHCLDYRNPHHTMAEAEPCCKRLKFDVSFFDDESDDDFYHSEPPIDQSTANQLPLIEDEEYTNPFDSLPNEVIQEICMMLSLRDKMSLRLVNRRLYMICSDPYLWRNVVIDDAYHKTNAPFIKSALQTCRPHVQSLSLRGELPFSKYQRMILTCKNIHTLNLYGFQISFNALEKIHSGLHHLQFLSLTYIDQIPLSNDRFFIIIAKLKKVALVYNNFEAEKLFEKWLLNNCLPQIFIIISAHGKEYWSRSRNIHHLPNITHSAYFAAYRKFCQPLDFDFYDVPEYSLEIGPNSSESVAVTANGKLMITMRDMITPINNDVNKRYAVFKDEAVTPGLSVYTSQYGVNITVLGFSSVTVSLESFRMIVKETPNILEVSLKGSIISDSLDAYIVPLFVHCLKLRGLNVSCFSTSSRDSINFDMEHFWNLLSKMKYLESLSVDCCSLSPLTPNSQHDGRSARVLTDRELAVKERVIGHIKRMTRLKSLYVIETIEIEELDYFINQHLLSMISNLESLLYLEINISYAADIASLYSCNMNFVEGLERILQKCQKLSFLVINFTSVKAIELPVDPALYGNLTHLWLGCQHGPITVEFGSALAINSKKKLKHLLLGHRYFDIELDVLDKINEGNLITFYELGSDKCFYRHDRNTCKKRGKLSELDFYIGKFPFLH

>PITRM1_truncated

MAAQLFSLPYVRRGIRLIHLSNPVFNSARREDVSKLSVAVNDRINGYRVTGVTDVPDFNLKAIELLHESTKAQHLHLARQDSNNVFGVGFRTTPMDSTGICHILEHTTLCGSAHYPVRDPFFKMLTRSLATFMNAFTANDWTFYPFSTQNYNDYRNLLSVYCDCMFHPNLKNMDFHHSIHHQ

>AmpOAS1Ca

MANPMPHVGTLSRGQVAQKVRLSIIAVRAVCSLLKQHFRTDSGIAVSEIVEGGSLGYGTYVPMKFDIDLVIYSTSLTADKLCDRDKRKKFLSDLQECVKVHLKGNYIADKMTENSVQFKYQFSPDNTIDVDVLISPMWGSPVELSQYVSKNCPTEFFHLSAGAAKWQKKFMSHQTLRVKEYIKRAKYWSLTVNWEAIGGRKPTSYMMSLLVIKAAEPFEIHMKGRLRQNFKKQLAKEITKNVKTLVKKFETIEILFDSDYPFPNALLPSPPRVVDPANICNNLYETGFGKLRRDEDSEKYWKKFAENIDTLDLSVAFEHRGFYSDVPAFGKSYQPKY

>AmpOAS1Cb

MANPMPHVGTLSRGQVAQKVRLSIVAVRAVCSLLKQHFRTDSGIAVSEIVEGGSLGYGTYVPMKFDIDLVIYSISLTADKLCDRDKRKKFLSDLQECVKVHLKGNYIADKMTENSVQFKYQFSPDNTIDVDVLISPMWGSPVELSQYISKNCPMEFFHLSAGAAKWQKKFMSHQTLRVKEYIKRAKYWSLTVNWEVIGGRKPTSYMMSLLVIKAAEPFKIHLKGKLTRYLRRQLPKDITENVKTLVKNFETIDISFDSDYHFPNALLPSPPRVVDPANICNNLYETGFGKLRRDEDSEKYWKKFAENIDTLDLSVAFEHRGFYSDVPAFGKSHQPKY

>AmpOAS1Cc

MANPMPQVGTLSKRQVIKKGKQSRRAVRTVCSLLKEHFRADCGIAVSEIVQGGSLGYGTYVPMKFDIDLVIYSTSLTADKLCDGDGRNELLLALQECIKIHLKKKYIEDKKTENSVQFKYQFSPDNTIDVDVLISPMWDDPVELSQYISRNCPNEFFHLSAGAAKWQKQFMCQQTSRVKEYIKRAKYWSLTVNWEKVEGRKPTSYMISLLVIKATEDFKDRMTGKLGKSVKRKLARRDWRQSSKNLLSLMILEIIFGSVFFESVSDFPIALLPPLPRVVDPANICNNLYKTGFGKLRRDEDSEKYWKKFAENIDTLDLSVAFEHRGFYSDAPTFGRSYHPNY

>Aldo/ketolase 2

MEASCKLLYKEIGSTGLRLCCLSLGGSGYGGIYGEYDEKKAEEALRSSLDKGVNYIDTSPFYGEGRSESFLGRVLSRVPRDKYYIGTKVGRYFWDVKKRFDFSRETILKGFEESLKRLQLEHVDILQLHDVEFAPSLDIIMNEALPAIQELKDKGFCRAIGITGYPIGPLKEIIAHSHHIKIDSVLSYARLTLNDNSLKDHFDFFKSHGVSIINASPVSMGLLTSEGPQWWNPALPYIKDKCKEAVEYCNSKGVDITRLAVNYSTSFDEVVTTLVAISDTQMLDRNLSAVLSPMTEKEKKVAGDLEKFFSRLPQRHWEGVELKEYWETNWKL

>Aldo/ketolase 1

MATASRSLPMLYRDLGRTGMRLSIVGLGGSGWGKNYGPYDEKEAVRAFHHALDRGVNYIDTAYWYAQGHSEKFLGQHLLPTVSRDRYFIATKVGRYELDLPNMFNFTAERVTKSLEESLRRLQLSEVDIIQIHDVEFSLSVNQILHETLPALERLKEKGLCRHIGITGYPLGPLKEVISQSSVQIDSILSYCRLSLNDWTLKNEFQFFKENKVPIINACPVSMGLLTEGPVQDWHPSLPWIRDACNRAVQYCREQGVDITKLALHYSTSFEEAVTTLVSMCDTEMVDKNLDSALSPLTPHEEGVMQHLLDNVFGDMTQRHWEGVELVSYWNATRK

>Coiled-coil domain ATPase

KEDKEKDTRPICPFSSPPSSLLLFEIIVQSSVPSLFRNCPSTEGIMSSQEESGSSLNNPSLHFPVPVTSKDATTPDEETRSLQPPTTREQPQAVHESIPHKPPPTAKVAPLVGGSSSAAIITSQRSTTPHEGTSVVGTPRRKGNKLDVLSSSFSCVSLSPTRTGGGSERSSPVKELLHADRVRNLMSLLTEKSQIIEKQKNTITRLQQDCKKKDSQLRLLTLPQANEGNVGGEANVGLSAPVKLDILSRKLDEAYYELEKAKSESDVMRDKISDKDSHISQLETDADRYKAKIKLLEDKIVKLEGYFGNTPTMEEHYSMKEQVTELHSQNILLSERLREKEDKIMAQIDELRDKETTLKEMSINIRDLKQDNSDLRLQCETSDRRLVELHSDISQYKIELMKSKQQITDLEEKNRQDLRDMRERHKLRFQEATAKLKEQYKTVTKRNRQLELQLLKNSDVMTALNNELKLSQGTVADLKSSFGDLVKQNQILMEKNYGLQEPPEVGRSGIVSVSPVSDGSDESLQALSQEVSQCVEEVDSLVSLASSIFEGKEPDMSLLLGGGATGNGDHKTDSELDPARKYEQQLTQVQKNRKEIERLREKVTDHFASKVANECHLQ

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