Colony kin structure in PolistesTable S3Seppä et al. - 1 of 5

Supplementary Table S3

Title: Colony kin structure and breeding patterns in the social wasp, Polistes biglumis.

Authors: P. Seppä, J. Fogelqvist, N. Gyllenstrand and M.C. Lorenzi

Correspondence:Perttu Seppä e-mail: ;
phone: -358-9-1915700; fax: -358-9-19157694

Table S3:Composition and breeding structure of Polistes biglumis nests. The first row of each nest shows the results at nest level, and the following lines for each matriline. The number after the nest number (in parenthesis) in Montgenèvre/96 nests is the number of cells in the nest; #FOBS is the number of breeding females sampled, the letters in parenthesis give the observed changes in the breeder composition in the Montgenèvre/96 nests (u=usurped nest; j=joined nest); when a change in the breeder composition was observed, we expected to find more than one matriline in the nests. ML are the matrilines found in the genetic analysis, breeding females are numbered in the order of appearance to the nest; females that were sampled are bolded; #PL is the mating frequency of each female. #E, #L, #P and #A are the numbers of eggs, larvae, pupae and adult brood in each nest, respectively (first row), and assigned for each breeding female (rows below), separately for females and males. SR is the sex ratio (%) as the numerical proportion of males in the brood. Notes give the most plausible interpretation of the colony kin structure and foundress turnover.

Table S3 continues

Nest / #FOBS / ML / #PL / #E
f, m / #L
f, m / #P
f, m / #
f, m / SR / Notes
Montgenèvre/96
1 (41) / 1 / 29 / 4 / 4 / Multiple matrilines: Usurpation and joining/
multiple usurpations
1 / 2 / 2, 0 / Original foundress, disappears
2 / 1 / 2, 0 / Usurper1, takes over
3 / 6 / 19, 1 / Joiner/Usurper 2, disappears later
2 (18) / 1 / 13 / 0 / Single matriline
1 / 1 / 12, 0
8 (37) / 1 (u) / 2 / 6 / 12 / Multiple matrilines: Joining
1 / 1 / 1, 0 / 5, 1 / Original foundress, nest owner
2 / 1, 0 / Joiner
9 (33) / 1 (u) / 15 / 2 / 5 / 67 / Multiple matrilines: Usurpation
1 / 0, 2 / 0, 5 / Original foundress, disappears
2 / 1 / 4, 1 / Usurper, takes over
11 (48) / 1 (u) / 21 / 9 / 7 / 23 / Multiple matrilines: Usurpation
1 / 2 / 4, 3 / Original foundress, disappears
2 / 1 / 13, 1 / 6, 3 / Usurper, takes over

Table S3 continues

12 (41) / 2 (u, j) / 8 / 6 / 2 / 29 / Multiple matrilines: Usurpation and joining
1 / 4 / 3, 5 / 6, 0 / 2, 0 / Original foundress, disappears
2 / Usurper, no own offspring yet
3 / Joiner, no own offspring yet
15 (35) / 1 / 2 / 4 / 1 / 43 / Multiple matrilines: Usurpation
1 / 1 / 1, 3 / 1, 0 / Original foundress, disappears
2 / 1 / 2, 0 / Usurper, takes over
17 (46) / 1 / 19 / 15 / 44 / Single matriline
1 / 1 / 15, 2 / 3, 12
20 (57) / 1 (u) / 29 / 10 / 1 / 28 / Multiple matrilines: Multiple usurpations
1 / 1 / 3, 0 / 1, 0 / Original foundress, disappears
2 / 3 / 13, 8 / 4, 3 / Usurper 1, takes over
3 / 1 / 7, 0 / Usurper 2, disappears later
21 (13) / 1 / 10 / 70 / Single matriline
1 / 2 / 3, 7
ns (29) / 1 (u) / 1 / 1 / 3 / 20 / Multiple matrilines: Usurpation and joining
1 / 1, 1 / Original foundress, disappears
2 / 2 / 1, 0 / 1, 0 / Usurper, takes over
3 / 1, 0 / Joiner

Table S3 continues

Montgenèvre/01
M1 / 1 / 3 / 7 / 20 / Single matriline
1 / 1 / 1, 2 / 7, 0
M2 / 1 / 16 / 88 / Single matriline
1 / 2 / 2, 14
M3 / 1 / 13 / 1 / 64 / Multiple matrilines: Usurpation
1 / 1, 0 / Original foundress, disappears
2 / 1 / 4, 9 / Usurper, takes over
M4 / 1 / 16 / 38 / Multiple matrilines: Joining
1 / 2 / 9, 6 / Original foundress, nest owner
2 / 1, 0 / Joiner
Ferrere
S1 / 1 / 13 / 4 / 1 / 1 / 74 / Single matriline
1 / 1 / 3, 10 / 0, 4 / 1, 0 / 1, 0
S2 / 1 / 1 / 5 / 2 / 1 / 29 / Multiple matrilines: Joining
1 / 1 / 0, 1 / 1, 1 / 2, 0 / 1, 0 / Original foundress, disappears
2 / 1, 0 / Joiner

Table S3 continues

S3 / 1 / 9 / 71 / Single matriline
1 / 1 / 2, 5
S4 / 1 / 12 / 4 / 62 / Single matriline
1 / 2 / 2, 10 / 4, 0
S5 / 1 / 5 / 7 / 4 / 20 / Single matriline
1 / 1 / 4, 1 / 4, 2 / 4, 0
S6 / 1 / 12 / 6 / 39 / Multiple matrilines: Usurpation
1 / 1 / 6, 0 / Original foundress, disappears
2 / 1 / 5, 7 / Usurper, takes over
S7 / 1 / 9 / 1 / 90 / Single matriline
1 / 1 / 1, 9
S8 / 1 / 6 / 2 / 88 / Single matriline
1 / 1 / 1, 5 / 0, 2
S9 / 1 / 9 / 8 / 38 / Multiple matrilines: Usurpation
1 / 3 / 2, 0 / 3, 5 / Original foundress, disappears
2 / 2 / 5, 1 / Usurper