82458 / EX-E2265-M06 / PF32207y / 2448
GC and CF clone
Template BC041888 has two dis-matched region compared to GC-E2265 NM_014857.3 & NM_014857.4.
Please make a CF clone from it.
BC041888 RABGAP1L MGC27813 IRAT-61-h-2 pBluescriptR ampicillin resistant available(RZPD) OK
BC041888 RABGAP1L OG11431 CCSB-81106-F-3 pENTR223 spectinomycin resistant available(ORFeome) ORFeome_clone,without_SC NA
>PF32207y GC-E2265 NM_014857.4 Homo sapiens RAB GTPase activating protein 1-like (RABGAP1L), transcript variant 1, mRNA. Length:2448 BP
aaggagtttaaacaccATGGAGGTCAGAGCTTCATTACAGAAGGTTAGTGGATCATCTGATTCTGTGGCTACAATGAACAGTGAAGAATTTGTTTTGGTTCCTCAGTATGCAGATGATAATTCTACAAAACATGAAGAAAAACCTCAACTGAAGATAGTTTCTAATGGTGATGAACAATTGGAAAAAGCCATGGAAGAGATTTTGAGAGATTCCGAGAAAAGGCCAAGCAGTCTTCTTGTTGATTGTCAAAGTTCCAGTGAGATTTCAGACCATTCGTTTGGAGATATTCCAGCCAGCCAAACAAATAAGCCATCTCTTCAGTTAATTTTGGATCCGTCTAACACAGAAATTTCTACACCCAGACCATCTTCTCCAGGTGGACTACCTGAAGAAGATAGTGTTTTATTTAATAAACTGACCTACTTAGGATGTATGAAGGTTTCTTCCCCACGTAATGAAGTAGAGGCTTTACGGGCAATGGCAACCATGAAATCTTCCAGTCAATACCCCTTTCCTGTTACCCTGTATGTACCAAATGTTCCAGAAGGTTCTGTGAGAATTATAGACCAATCCAGCAATGTGGAGATAGCATCTTTTCCAATCTATAAGGTGTTATTCTGTGCACGTGGACATGACGGAACAACAGAGAGCAATTGCTTTGCATTTACAGAGAGTTCCCATGGTTCGGAAGAATTTCAGATACATGTTTTCTCCTGTGAAATTAAAGAGGCAGTAAGCAGAATTTTGTACAGTTTCTGTACAGCATTCAAACGTTCTTCCAGACAAGTGTCTGATGTTAAAGACTCAGTTATTCCTACCCCCGACAGTGATGTGTTTACCTTCAGTGTCTCCTTGGAGGTAAAAGAAGACGATGGAAAAGGAAACTTTAGCCCTGTGCCTAAGGATAGAGATAAATTTTATTTCAAATTAAAGCAAGGAATAGAGAAGAAGGTTGTGATTACAGTGCAGCAACTTTCTAACAAAGAATTAGCTATTGAAAGATGTTTTGGAATGTTATTAAGCCCAGGTCGAAACGTGAAGAACAGTGACATGCATTTACTGGATATGGAATCCATGGGAAAGAGCTATGATGGGAGAGCTTATGTCATCACTGGCATGTGGAACCCCAATGCACCAGTATTTCTGGCACTTAACGAGGAAACCCCAAAAGATAAGCAAGTATACATGACTGTGGCAGTGGATATGGTAGTCACAGAGGTGGTGGAGCCTGTTCGCTTTCTCCTGGAGACAGTAGTCCGTGTGTACCCTGCAAATGAGCGATTTTGGTATTTCAGCAGAAAGACTTTCACAGAGACTTTCTTCATGAGATTGAAACAGTCTGAGGGAAAAGGCCATACCAATGCTGGAGATGCAATATATGAGGTGGTGAGTCTACAGCGAGAGTCTGACAAGGAGGAACCAGTCACTCCTACTAGTGGAGGGGGTCCAATGTCACCCCAGGATGATGAAGCAGAAGAGGAGAGTGATAATGAACTCTCAAGTGGAACAGGTGATGTGTCTAAGGATTGTCCTGAGAAGATCCTGTATTCTTGGGGAGAGTTGCTAGGAAAATGGCACAGTAACCTTGGTGCACGACCGAAAGGGCTGTCTACTCTGGTGAAGAGTGGTGTCCCTGAAGCATTGAGGGCAGAGGTATGGCAGTTATTGGCAGGCTGCCATGACAACCAGGCAATGCTGGATAGATACCGAATTCTTATCACAAAGGACTCAGCCCAGGAGAGTGTTATTACTCGAGATATTCATCGTACATTTCCCGCACATGATTACTTTAAAGATACTGGAGGAGATGGTCAAGAATCGCTCTATAAGATCTGCAAGGCCTACTCTGTGTATGATGAAGACATTGGGTACTGTCAAGGGCAGTCTTTTCTTGCTGCTGTATTACTGCTGCATATGCCAGAGGAACAAGCATTCTGTGTTTTGGTGAAAATCATGTACGACTATGGTTTGAGAGACCTCTACAGAAACAACTTCGAAGATCTTCATTGCAAATTCTACCAGTTGGAGAGACTAATGCAGGAACAGCTACCGGACCTGCATAGCCATTTTTCTGATCTGAACCTGGAAGCTCATATGTATGCATCCCAGTGGTTTCTCACTCTTTTTACTGCCAAGTTCCCACTCTGCATGGTGTTCCACATCATTGACTTACTGCTTTGTGAGGGTTTGAACATAATCTTTCATGTAGCTTTGGCTCTCCTAAAGACCTCAAAGGAAGACCTTCTGCAGGCTGATTTTGAAGGTGCTTTAAAGTTCTTTAGAGTTCAGCTTCCAAAGAGATACAGGGCAGAGGAAAATGCAAGAAGACTGATGGAGCAGGCTTGCAATATTAAAGTACCAACCAAGAAGCTGAAGAAATATGAGAAAGAATATCAGACAATGCGAGAGAGTCAGCTGCAACAGGAAGACCCAATGGATAGATACAAGTTTGTATATTTGTAcCTCGAGTGCGGCCGCA
PF32207y / GC-E2265 / 2448 / PF21748y / APBox423-09 / PR14128r / APBox294-08合成CF clone，PR做GC
IF:GAAGATAGTTTCTAATGGTGATGAACAAT
IR:CTTTGTGATAAGAATTCGGTATCTATCCAGC
GCTGGATAGATACCGAATTCTTATCACAAAG
PARAMETERS FOR TRIAL 21
------
Total Size Of Gene ...... 179 nt
Protein Residues ...... 0
Mutatable Residues ...... 0
Fixed Nucleotides ...... 179 nt
Degenerate Nucleotides ..... 0 nt
Oligo Size ...... 54 nt
Annealing Temp ...... 64 +/- 1*C
Oligo Concentration ...... 1.00E-7 M
Sodium Concentration ...... 5.00E-2 M
Mg2+ Concentration ...... 2.00E-3 M
Codon Frequency Threshold .. 10%
Repeat Threshold ...... 8 nt
Mispriming Threshold ...... 8/18 (6 exact) nt
------
The DNA sequence # 21 is:
------
1 AAGGAGTTTAAACACCATGGAGGTCAGAGCTTCATTACAGAAGGTTAGTGGATCATCTGA
61 TTCTGTGGCTACAATGAACAGTGAAGAATTTGTTTTGGTTCCTCAGTATGCAGATGATAA
121 TTCTACAAAACATGAAGAAAAACCTCAACTGAAGATAGTTTCTAATGGTGATGAACAAT
------
The oligonucleotide assembly is:
------
1 10 20 30 40 50 60
| | | | | | |
1 ---> 3 --->
1 AAGGAGTTTAAACACCATGGAGGTCAGAGC cagaaggttagtggatcatctga
******** ******** < repeat
ATTTGTGGTACCTCCAGTCTCGAAGTAATGTCTTCCAATCACCTAGTAGACT
<---
| | | | | | |
5 --->
61 ttctgtggctacaatgaacagtgaagaattt GGTTCCTCAGTATGCAGATGATAA
******** < repeat
AA ccgatgttacttgtcacttcttaaacaaaaccaaggagtcatacgtctactatt
2 <--- 4
| | | | | | |
121 TTCTACAAAACATGAAGAAAAACCTCAACT
ATGTTTTGTACTTCTTTTTGGAGTTGACTTCTATCAAAGATTACCACTACTTGTTA
<--- 6
------
The total codon usage score ...... 0.000
The total length score ...... 0.000
The total melting temperature score ... 0.000
The total repeat score ...... 3.575
The total pattern score ...... 0.000
The total mispriming score ...... 0.000
The total AT content score ...... 0.000
The total GC content score ...... 0.000
The total fixed gap score ...... N/A
The OVERALL score ...... 3.575
Tm Range # of Overlaps
------
<61 0
61 0
62 0
63 4
64 1
65 0
66 0
67 0
68 0
>=69 0
------
Tm Range = 1.5
Ovrlap Len Range # of Oligos
------
<21 0
21 0
22 1
23 0
24 1
25 2
26 0
27 1
28 0
29 0
30 0
>=31 0
------
Lowest Overlap = 22
Length Range # of Oligos
------
< 42 1
42- 43 0
44- 45 0
46- 47 0
48- 49 0
50- 51 0
52- 53 0
54- 55 4
56- 57 1
58- 59 0
60- 61 0
>= 62 0
------
Longest = 56
There are 2 repeats greater than 8 nt:
------
PR Pos1 = 6 Pos2 = 6 Size = 8 Seq1 = GTTTAAAC
Seq2 = GTTTAAAC
IR Pos1 = 52 Pos2 = 111 Size = 8 Seq1 = ATCATCTG
Seq2 = CAGATGAT
------
Sequence Patterns Screened (As Supplied By User)
------
None found
------
6 oligonucleotides need to be synthesized
------
1 AAGGAGTTTAAACACCATGGAGGTCAGAGC 30
2 AATCAGATGATCCACTAACCTTCTGTAATGAAGCTCTGACCTCCATGGTGTTTA 54
3 CAGAAGGTTAGTGGATCATCTGATTCTGTGGCTACAATGAACAGTGAAGAATTT 54
4 TTATCATCTGCATACTGAGGAACCAAAACAAATTCTTCACTGTTCATTGTAGCC 54
5 GGTTCCTCAGTATGCAGATGATAATTCTACAAAACATGAAGAAAAACCTCAACT 54
6 ATTGTTCATCACCATTAGAAACTATCTTCAGTTGAGGTTTTTCTTCATGTTTTGTA 56
CLUSTAL W (1.83) multiple sequence alignment
PF32207y ------
BC041888.1 GGCCTGGACGAGCGCGCTTCCGACCCGCCGCCACCGCCTCCTCCCCTCCTTCTCGGCCCG 60
::
PF32207y ------
BC041888.1 CCCCCTCCCTCGCCTCTTCCTGCCAGGCGGCCCTTCTCCCCTCCCCTCTCAGTTCCCTCC 120
::
PF32207y ------ATG-GA 5
BC041888.1 GCCCTCCTCGGGCTCCAGCGGTGGCGGAGCGAACGGGACCGGCCCGGCTTCAGAGCGCGA 180
* ** ::
PF32207y GGTCAGAGCTTCATTACAGAAGGTTAGTGGATCATCTGATTCTGTGGCTACAATGAACA- 64
BC041888.1 GGTGGAGGGTGGAACGCGGGCGCCTGAAGGAGTTGTTGTCTCGGCAGCGCCCGCGGAGAC 240
*** * * * * * * * *** ** ** * ** * * * * ::
PF32207y GTGAAGAATTTGTTTTGGTTCCTCAGTATGCAGATGATAATTCTACAAAACATGAAGAAA 124
BC041888.1 GTGAAGAGGTGGTTGTGG------GAAGAGAAGTTTGCAGAACTGAAATGGAGGTCAGAG 294
******* * *** *** * ** * * ** ** * * * ::
PF32207y AACCTCAACTGAAGATAGTTTCTAATGGTGATGAACAATTGGAAAAAGCCATGGAAGAGA 184
BC041888.1 CTTCATTACAGAAGATAGTTTCTAATGGTGATGAACAATTGGAAAAAGCCATGGAAGAGA 354
* ** ************************************************** ::
PF32207y TTTTGAGAGATTCCGAGAAAAGGCCAAGCAGTCTTCTTGTTGATTGTCAAAGTTCCAGTG 244
BC041888.1 TTTTGAGAGATTCCGAGAAAAGGCCAAGCAGTCTTCTTGTTGATTGTCAGAGTTCCAGTG 414
************************************************* ********** ::
==>conserved
PF32207y AGATTTCAGACCATTCGTTTGGAGATATTCCAGCCAGCCAAACAAATAAGCCATCTCTTC 304
BC041888.1 AGATTTCAGACCATTCGTTTGGAGATATTCCAGCCAGCCAAACAAATAAGCCATCTCTTC 474
************************************************************
PF32207y AGTTAATTTTGGATCCGTCTAACACAGAAATTTCTACACCCAGACCATCTTCTCCAGGTG 364
BC041888.1 AGTTAATTTTGGATCCGTCTAACACAGAAATTTCTACACCCAGACCATCTTCTCCAGGTG 534
************************************************************
PF32207y GACTACCTGAAGAAGATAGTGTTTTATTTAATAAACTGACCTACTTAGGATGTATGAAGG 424
BC041888.1 GACTACCTGAAGAAGATAGTGTTTTATTTAATAAACTGACCTACTTAGGATGTATGAAGG 594
************************************************************
PF32207y TTTCTTCCCCACGTAATGAAGTAGAGGCTTTACGGGCAATGGCAACCATGAAATCTTCCA 484
BC041888.1 TTTCTTCCCCACGTAATGAAGTAGAGGCTTTACGGGCAATGGCAACCATGAAATCTTCCA 654
************************************************************
PF32207y GTCAATACCCCTTTCCTGTTACCCTGTATGTACCAAATGTTCCAGAAGGTTCTGTGAGAA 544
BC041888.1 GTCAATACCCCTTTCCTGTTACCCTGTATGTACCAAATGTTCCAGAAGGTTCTGTGAGAA 714
************************************************************
PF32207y TTATAGACCAATCCAGCAATGTGGAGATAGCATCTTTTCCAATCTATAAGGTGTTATTCT 604
BC041888.1 TTATAGACCAATCCAGCAATGTGGAGATAGCATCTTTTCCAATCTATAAGGTGTTATTCT 774
************************************************************
PF32207y GTGCACGTGGACATGACGGAACAACAGAGAGCAATTGCTTTGCATTTACAGAGAGTTCCC 664
BC041888.1 GTGCACGTGGACATGACGGAACAACAGAGAGCAATTGCTTTGCATTTACAGAGAGTTCCC 834
************************************************************
PF32207y ATGGTTCGGAAGAATTTCAGATACATGTTTTCTCCTGTGAAATTAAAGAGGCAGTAAGCA 724
BC041888.1 ATGGTTCGGAAGAATTTCAGATACATGTTTTCTCCTGTGAAATTAAAGAGGCAGTAAGCA 894
************************************************************
PF32207y GAATTTTGTACAGTTTCTGTACAGCATTCAAACGTTCTTCCAGACAAGTGTCTGATGTTA 784
BC041888.1 GAATTTTGTACAGTTTCTGTACAGCATTCAAACGTTCTTCCAGACAAGTGTCTGATGTTA 954
************************************************************
PF32207y AAGACTCAGTTATTCCTACCCCCGACAGTGATGTGTTTACCTTCAGTGTCTCCTTGGAGG 844
BC041888.1 AAGACTCAGTTATTCCTACCCCCGACAGTGATGTGTTTACCTTCAGTGTCTCCTTGGAGG 1014
************************************************************
PF32207y TAAAAGAAGACGATGGAAAAGGAAACTTTAGCCCTGTGCCTAAGGATAGAGATAAATTTT 904
BC041888.1 TAAAAGAAGACGATGGAAAAGGAAACTTTAGCCCTGTGCCTAAGGATAGAGATAAATTTT 1074
************************************************************
PF32207y ATTTCAAATTAAAGCAAGGAATAGAGAAGAAGGTTGTGATTACAGTGCAGCAACTTTCTA 964
BC041888.1 ATTTCAAATTAAAGCAAGGAATAGAGAAGAAGGTTGTGATTACAGTGCAGCAACTTTCTA 1134
************************************************************
PF32207y ACAAAGAATTAGCTATTGAAAGATGTTTTGGAATGTTATTAAGCCCAGGTCGAAACGTGA 1024
BC041888.1 ACAAAGAATTAGCTATTGAAAGATGTTTTGGAATGTTATTAAGCCCAGGTCGAAACGTGA 1194
************************************************************
PF32207y AGAACAGTGACATGCATTTACTGGATATGGAATCCATGGGAAAGAGCTATGATGGGAGAG 1084
BC041888.1 AGAACAGTGACATGCATTTACTGGATATGGAATCCATGGGAAAGAGCTATGATGGGAGAG 1254
************************************************************
PF32207y CTTATGTCATCACTGGCATGTGGAACCCCAATGCACCAGTATTTCTGGCACTTAACGAGG 1144
BC041888.1 CTTATGTCATCACTGGCATGTGGAACCCCAATGCACCAGTATTTCTGGCACTTAACGAGG 1314
************************************************************
PF32207y AAACCCCAAAAGATAAGCAAGTATACATGACTGTGGCAGTGGATATGGTAGTCACAGAGG 1204
BC041888.1 AAACCCCAAAAGATAAGCAAGTATACATGACTGTGGCAGTGGATATGGTAGTCACAGAGG 1374
************************************************************
PF32207y TGGTGGAGCCTGTTCGCTTTCTCCTGGAGACAGTAGTCCGTGTGTACCCTGCAAATGAGC 1264
BC041888.1 TGGTGGAGCCTGTTCGCTTTCTCCTGGAGACAGTAGTCCGTGTGTACCCTGCAAATGAGC 1434
************************************************************
PF32207y GATTTTGGTATTTCAGCAGAAAGACTTTCACAGAGACTTTCTTCATGAGATTGAAACAGT 1324
BC041888.1 GATTTTGGTATTTCAGCAGAAAGACTTTCACAGAGACCTTCTTCATGAGATTGAAACAGT 1494
************************************* ********************** ::
=>conserved
PF32207y CTGAGGGAAAAGGCCATACCAATGCTGGAGATGCAATATATGAGGTGGTGAGTCTACAGC 1384
BC041888.1 CTGAGGGAAAAGGCCATACCAATGCTGGAGATGCAATATATGAGGTGGTGAGTCTACAGC 1554
************************************************************
PF32207y GAGAGTCTGACAAGGAGGAACCAGTCACTCCTACTAGTGGAGGGGGTCCAATGTCACCCC 1444
BC041888.1 GAGAGTCTGACAAGGAGGAACCAGTCACTCCTACTAGTGGAGGGGGTCCAATGTCACCCC 1614
************************************************************
PF32207y AGGATGATGAAGCAGAAGAGGAGAGTGATAATGAACTCTCAAGTGGAACAGGTGATGTGT 1504
BC041888.1 AGGATGATGAAGCAGAAGAGGAGAGTGATAATGAACTCTCAAGTGGAACAGGTGATGTGT 1674
************************************************************
PF32207y CTAAGGATTGTCCTGAGAAGATCCTGTATTCTTGGGGAGAGTTGCTAGGAAAATGGCACA 1564
BC041888.1 CTAAGGATTGTCCTGAGAAGATCCTGTATTCTTGGGGAGAGTTGCTAGGAAAATGGCACA 1734
************************************************************
PF32207y GTAACCTTGGTGCACGACCGAAAGGGCTGTCTACTCTGGTGAAGAGTGGTGTCCCTGAAG 1624
BC041888.1 GTAACCTTGGTGCACGACCGAAAGGGCTGTCTACTCTGGTGAAGAGTGGTGTCCCTGAAG 1794
************************************************************
PF32207y CATTGAGGGCAGAGGTATGGCAGTTATTGGCAGGCTGCCATGACAACCAGGCAATGCTGG 1684
BC041888.1 CATTGAGGGCAGAGGTATGGCAGTTATTGGCAGGCTGCCATGACAACCAGGCAATGCTGG 1854
************************************************************
PF32207y ATAGATACCGAATTCTTATCACAAAGGACTCAGCCCAGGAGAGTGTTATTACTCGAGATA 1744
BC041888.1 ATAGATACCGAATTCTTATCACAAAGCAACAGATGAAATTTAGTCTTACA--CCGAGACA 1912
************************** * * *** *** ***** * ::
PF32207y TTCATCGTACATTTCCCGCACATGATTACTTTAAAGATACTGGAGGAGATGGTCAAGAAT 1804
BC041888.1 GAC------TATT-----CACCTAGTTAAATATGAAGGGTCTATGAAGGTCTCCATGA-- 1959
* *** *** * *** * * * ** * ** ** ::
PF32207y CGCTCTATAAG-ATCTGCAAGGCCTACTCTGTGTATGATGAAGACATTGG--GTACTGTC 1861
BC041888.1 CACCCTGTAACCAGCTGCAAT-TTGACATTAGACTTGATGTTCTGATTACTTATACTTTT 2018
* * ** *** * ****** ** * ***** *** **** * ::
PF32207y AAGGGCAGT-CTTTTCTTGCTGCTGTATTACTGCTGCATATGCCAGAGGAACAAGC-ATT 1919
BC041888.1 TGTTTTAGTTCCTTTCCTGAGCCTGAATTGTACAAATATGTCCTAAAACACCAACTTATT 2078
*** * **** ** *** *** ** * * * * * *** *** ::
PF32207y CTGTGTTTTGGTGAAAATCATGTACGACTATGGTTTGAGAGACCTCTACAGAAACAACTT 1979
BC041888.1 AAGAGGCTTAATGCCTGTTAAATGCGTCAGTGCTCTTGGCAGCTTA-GCATGAGTGCTTT 2137
* * ** ** * * * ** * ** * * * * * ** * ** ::
PF32207y CGAAGATCTTCATTGCAAATTCTACCAGTTGGAGAGACTAATGCA-GGAACAGCTACCGG 2038
BC041888.1 C-----TTTGCATGGGAGGTT---GCAGTTT-AGAAGCAACTTTATGGATCATTTCCCAG 2188
* * * *** * * ** ***** *** * * * * *** ** * ** * ::
PF32207y ACCTGCATAGCCATTTTTCTGATCTGAACCTGGAAGC-TCATATGTATGCATCCCAGTGG 2097
BC041888.1 GTTGATGTTACATATGTTCATTTTACAACCTGTGTGCATAATCTCTCAGGGTGAAGGTAA 2248
* * * *** * ****** ** * ** * * * * ** ::
PF32207y TTTCTCACTCTTTTTA-CTGCCAAGTTCCCACTCTGCA---TGGTGTTCCACATCATTGA 2153
BC041888.1 TTAAGAGAAAGCATAATCTACAGAATTGAAGCCAAGTAATCTAAGATTCTGT-TTTTAAA 2307
** * * ** * * ** * * * * *** * * * ::
PF32207y CTTACTGCTTTGTGAGGGTTTGAACATAATCTTTCATGTAGCTTTGGCTCTCCTAAAGAC 2213
BC041888.1 CTCTTTGCTATATAACATTAGTTAAGTTGTTTACCCTCTTAATTTATCCATTTGTAAAA- 2366
** **** * * * * * * * * * * * *** * * ** * ::
PF32207y CTCAAAGGAAGACCTTCTGCAGGCTGATTTTGAAGGTGCTTTAAAGTTCTTTAGAGTTCA 2273
BC041888.1 -TGATAGTTTTTGTTTTTAAAGTGCTCTATTACCCTTACTTTAAAAGTCCT----GTCTA 2421
* * ** ** * ** * ** * ******* ** * ** * ::
PF32207y GCTTCCAAAGAGATACAGGGCAGAGGAAAATGCAAGAAGACTGA--TGGAGCAGGCTTGC 2331
BC041888.1 CCATATATTTAAAACTATAAGTGGAAATAATGAAGAAACTCTGAACTTGCTTTTGCTTAT 2481
* * * * * * * * **** * ** **** * * **** ::
PF32207y AATATTAAAGTACCAACCAAGAAGCTGAAGAAATATGAGAAAGAATATCAGACAATGCGA 2391
BC041888.1 A-TATGGATATATCATATTATATGCCTAAGATTGGAGATAAATTTTATAACCTGATTTTC 2540
* *** * ** ** * * ** **** ** *** *** * ** ::
PF32207y GAGAGTCAGC-TGCAACAGGAAG-ACCCAATGGATAGATACAAGTTTGTATATTTGTAG- 2448
BC041888.1 TCATGGCAGTGTACAAATGAAATTATTCTGTAAGTTTTT-CAAAACAGTAAAATCACTAT 2599
* *** * *** * ** * * * * * *** *** * * ::
PF32207y ------
BC041888.1 TTTTCAAAAAAAAAAAAAAAA 2620
::
CLUSTAL W (1.83) multiple sequence alignment
PF32207y MEVRASLQKVSGSSDSVATMNSEEFVLVPQYADDNSTKHEEKPQLKIVSNGDEQLEKAME 60
BC041888.1 MEVRASLQ------KIVSNGDEQLEKAME 23
********. :.::.: :: .:.. .. :...::...... *************** ::
PF32207y EILRDSEKRPSSLLVDCQSSSEISDHSFGDIPASQTNKPSLQLILDPSNTEISTPRPSSP 120
BC041888.1 EILRDSEKRPSSLLVDCQSSSEISDHSFGDIPASQTNKPSLQLILDPSNTEISTPRPSSP 83
************************************************************
PF32207y GGLPEEDSVLFNKLTYLGCMKVSSPRNEVEALRAMATMKSSSQYPFPVTLYVPNVPEGSV 180
BC041888.1 GGLPEEDSVLFNKLTYLGCMKVSSPRNEVEALRAMATMKSSSQYPFPVTLYVPNVPEGSV 143
************************************************************
PF32207y RIIDQSSNVEIASFPIYKVLFCARGHDGTTESNCFAFTESSHGSEEFQIHVFSCEIKEAV 240
BC041888.1 RIIDQSSNVEIASFPIYKVLFCARGHDGTTESNCFAFTESSHGSEEFQIHVFSCEIKEAV 203
************************************************************
PF32207y SRILYSFCTAFKRSSRQVSDVKDSVIPTPDSDVFTFSVSLEVKEDDGKGNFSPVPKDRDK 300
BC041888.1 SRILYSFCTAFKRSSRQVSDVKDSVIPTPDSDVFTFSVSLEVKEDDGKGNFSPVPKDRDK 263
************************************************************
PF32207y FYFKLKQGIEKKVVITVQQLSNKELAIERCFGMLLSPGRNVKNSDMHLLDMESMGKSYDG 360
BC041888.1 FYFKLKQGIEKKVVITVQQLSNKELAIERCFGMLLSPGRNVKNSDMHLLDMESMGKSYDG 323
************************************************************
PF32207y RAYVITGMWNPNAPVFLALNEETPKDKQVYMTVAVDMVVTEVVEPVRFLLETVVRVYPAN 420
BC041888.1 RAYVITGMWNPNAPVFLALNEETPKDKQVYMTVAVDMVVTEVVEPVRFLLETVVRVYPAN 383
************************************************************
PF32207y ERFWYFSRKTFTETFFMRLKQSEGKGHTNAGDAIYEVVSLQRESDKEEPVTPTSGGGPMS 480
BC041888.1 ERFWYFSRKTFTETFFMRLKQSEGKGHTNAGDAIYEVVSLQRESDKEEPVTPTSGGGPMS 443
************************************************************
PF32207y PQDDEAEEESDNELSSGTGDVSKDCPEKILYSWGELLGKWHSNLGARPKGLSTLVKSGVP 540
BC041888.1 PQDDEAEEESDNELSSGTGDVSKDCPEKILYSWGELLGKWHSNLGARPKGLSTLVKSGVP 503
************************************************************
PF32207y EALRAEVWQLLAGCHDNQAMLDRYRILITKDSAQESVITRDIHRTFPAHDYFKDTGGDGQ 600
BC041888.1 EALRAEVWQLLAGCHDNQAMLDRYRILITKQQMKFSLTPR------543
******************************:. : *: .*. : .: . ..:..... ::
PF32207y ESLYKICKAYSVYDEDIGYCQGQSFLAAVLLLHMPEEQAFCVLVKIMYDYGLRDLYRNNF 660
BC041888.1 ------
.: . ..: : ...... : :: ....: ...... ::
PF32207y EDLHCKFYQLERLMQEQLPDLHSHFSDLNLEAHMYASQWFLTLFTAKFPLCMVFHIIDLL 720
BC041888.1 ------QTIHLV 549
...... : :. . .: ::. : ::. . . : *.*: ::
PF32207y LCEGLNIIFHVALALLKTSKEDLLQADFEGALKFFRVQLPKRYRAEENARRLMEQACNIK 780
BC041888.1 KYEGS------MKVSMTPCNQLQFD-----IRLDVLITY------TFCFSS 583
** . : : :*.* * :*:.: . :*::: * :...: . * . ::
PF32207y VPTKKLKKYEKEYQTMRESQLQQEDPMDRYKFVYL 815
BC041888.1 FPEPELYKYVLKHQLIKRLNAC------605
.* :* ** ::* ::. : ...... ::