Table S1.Extractability of major tea constituents of tea roots [seedling roots (RA), whole root (RB) and in roots hairs (RC)] (mg/g) in 70% acetone extracts

Compounds / RA / RB / RC
Theanine / 58.17 ± 0.110 / 2.14 ± 0.020 / 13.19 ± 0.060
GC / ND / ND / ND
EGC / 0.10 ± 0.009 / ND / ND
Catechin / 0.01 ± 0.001 / 0.01 ± 0.002 / 0.01 ± 0.001
Caffeine / 0.29 ± 0.003 / ND / ND
EC / 0.40 ± 0.001 / 1.24 ± 0.003 / 0.92 ± 0.009
EGCG / 0.42 ± 0.004 / 1.09 ± 0.005 / 1.12 ± 0.006
GCG / 0.63 ± 0.002 / 1.56 ± 0.005 / 1.51± 0.003
ECG / 3.50 ± 0.001 / ND / ND

Results are expressed as mg/g and mean of three replicates ± SD

GC; Gallocatechin, EGC; Epigallocatechin, EC; Epicatechin; EGCG;

Epigallocatechin gallate, GCG; Gallocatechingallate, ECG; Epicatechingallate,

CG; Catechingallate

ND = Not detected

Table S2.Extractability of major tea constituents of mature leaves, flowers and fruits in 70% acetone and hot water

Compounds / 70% Acetone / Hot water
Mature Leaves / Flowers / Fruits / Mature Leaves / Flowers / Fruits
Theanine / 2.42 ± 0.020 / 8.35 ± 0.070 / 5.18 ± 0.121 / 1.16 ± 0.020 / 4.56 ± 0.030 / 4.72 ± 0.021
GC / 0.71 ± 0.010 / 0.84 ± 0.010 / 0.99 ± 0.030 / 1.21 ± 0.031 / 0.78 ± 0.001 / 1.66 ± 0.012
EGC / 5.27 ± 0.090 / 2.50 ± 0.031 / 5.27 ± 0.102 / 1.83 ± 0.031 / 1.10 ± 0.002 / 5.05 ± 0.011
Catechin / 0.51 ± 0.007 / 0.67 ± 0.001 / 0.75 ± 0.030 / 0.11 ± 0.002 / 0.22 ± 0.011 / 0.09 ± 0.001
Caffeine / 6.83 ± 0.110 / 7.77 ± 0.080 / 11.27 ± 0.050 / 3.66 ± 0.007 / 3.36 ± 0.050 / 7.26 ± 0.023
EC / 2.49 ± 0.020 / 2.75 ± 0.060 / 3.41 ± 0.040 / 1.42 ± 0.020 / 0.75 ± 0.010 / 2.94 ± 0.040
EGCG / 17.05 ± 0.030 / 14.96 ± 0.040 / 13.68 ± 0.010 / 8.60 ± 0.012 / 5.35 ± 0.003 / 5.73 ± 0.044
GCG / 0.34 ± 0.011 / ND / 0.67 ± 0.001 / ND / ND / 6.29 ± 0.060
ECG / 4.74 ± 0.070 / 8.79 ± 0.120 / 3.03 ± 0.020 / 4.73 ± 0.081 / 3.90±0.010 / 1.76 ± 0.030

Results are expressed as mg/g and mean of three replicates ± SD

GC; Gallocatechin, EGC; Epigallocatechin, EC; Epicatechin; EGCG; Epigallocatechin gallate, GCG;

Gallocatechingallate, ECG; Epicatechingallate, CG; Catechingallate

ND = Not detected

Table S3.Total phenolics andIC50 values against DPPH and ABTS radicals of purified extracts obtained from underutilized tea parts.

Extracts / TPC
% / DPPH ABTS
IC50 (µg/mL)
Leaves / 89.7 / 7.00 / 5.13
Flowers / 83.8 / 8.67 / 5.57
Fruits / 94.1 / 5.91 / 4.48
Trolox / - / 13.8 / 10.30

TPC - Total phenolics content as gallic acid equivalent

DPPH - 2,2-Diphenyl-1-picrylhydrazyl

ABTS - 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)

Table S4. Effect of various Tea extracts on Human Oral Cancer cells (KB)

% Cytotoxicity on KB cells
Test Extracts / Concentration / 24 hr / 48 hr / 72hr
Vinblastine / NA / 2μg/ml / 74.2±0.3 / 81.1±1.4 / 70.9±1.8
Tea / Flower / 25μg/ml / 5.3±0.4 / 22.1±2.1 / 9.8±0.21
50μg/ml / 8±3 / 13.1±0.9 / 18.1±2.75
100μg/ml / 19.1±0.77 / 23.6±2.2 / 26.7±2.2
200μg/ml / 12.5±1.6 / 15±0.4 / 16.8±3.7
Leaf / 25μg/ml / 4.8±0.31 / 16.4±1.4 / 13±0.91
50μg/ml / 6.2±0.95 / 10.6±1.6 / 24.7±0.68
100μg/ml / 22.5±2.9 / 17.7±2.3 / 30.8±1.6
200μg/ml / 20.2±0.6 / 33.6±0.36 / 26.8±1.1
Fruit / 25μg/ml / 32.8±2.6 / 10.8±2.87 / 5.1±0.62
50μg/ml / 71.6±1.3 / 16.6±0.51 / 14±2.7
100μg/ml / 73.2±0.9 / 8.3±0.64 / 25.3±3.1
200μg/ml / 74.9±0.23 / 14.6±1.8 / 21.1±0.43

Table S5.: Effect of various Tea extracts on Mice splenocyte proliferation

% Lymphocyte Proliferation
Test Extracts / Concentration / 24 hr / 48 hr / 72hr
Tea / Flower / 25μg/ml / 0±0.5 / 13.6±2.6 / 0±2.2
50μg/ml / 0±0.22 / 6.7±1.75 / 2.7±1.8
100μg/ml / 14.2±1.4 / 17.5±0.82 / 4.8±0.4
200μg/ml / 30.7±3.76 / 15.9±2.54 / 43.3±0.2
Leaf / 25μg/ml / 0±0.41 / 14±0.26 / 0±0.71
50μg/ml / 0±0.12 / 0±1.11 / 0±3.6
100μg/ml / 0±2.5 / 26.4±1.7 / 0±3.1
200μg/ml / 37.8±1.9 / 22.5±0.6 / 29.8±2.7
Fruit / 25μg/ml / 0±0.9 / 0±2.1 / 7.3±3.8
50μg/ml / 0±1.22 / 11.3±2.8 / 5.3±1.28
100μg/ml / 0±1.5 / 29.4±1.8 / 32±2.77
200μg/ml / 63.4±0.7 / 34.2±1.6 / 33.4±0.46

Figure S1. Major bioactive constituents in various parts of tea plant

Figure S2. Correlation between (a) ABTS and DPPH (b) Total phenolics and antioxidant activity

(a)(b)

Figure S3. Effect of purified extracts on KB (Oral cancer cells) and mice splenocytes