Table 1. Oligonucleotide primers used for amplification of rat Abcg5 and Abcg8

Primer / Sense primers (5’ to 3’) / 5’nt positiona /

Primer

name / Antisense primers (5’ to 3’) / 5’nt position a
/

Abcg5

RG5-F1 / CCCCATAGATGTCTGCCTTTCG / IVS1+716as / RG8-R1 / CACTTCCACCCTGATCTGCTAGAC / IVS1+120b
RG5-F2 / CCCTCTGTAGATGGAGAAGGCTTG / IVS1-354 / RG5-R2 / TTCTTAAACACCGAGCTTAGACCG / IVS2+79
RG5-R3 / CACATACAGGGGTTCTTCAGACTTG / IVS2-219 / RG5-R4 / CTTAGGTTGGAGAGGTCACGTTTC / IVS4+177
RG5-F5 / GGGGTCATCACCAGTCCATAAAC / IVS4-77 / RG5-R5 / TGCGAGGGAATGTGCTACCAC / IVS5+324
RG5-F6 / CAGGTCAGGTGCTTTCTTCTGG / IVS5-176 / RG5-R6 / ACTGGCGTGTGCTCTTTACCC / IVS6+113
RG5-F7 / TGATGCTGGGGCTCAGAGGC / IVS6-86 / RG5-R8 / CACCACCTGCTTATTCCTCATTAGG / IVS8+143
RG5-F9 / GACATCGGTGGACACCCAAAG / IVS8-310 / RG5-R9 / CCCAAGCTGAACTTGAGGTCAG / IVS9+1486
RG5-F10 / TGACCTCAAGTTCAGCTTGGGTAG / IVS9-82 / RG5-R10 / GGGGTTGCTCATAATAAGTTGGC / IVS10+140
RG5-F11 / GATAGTGTGCGGAGAGAGAACTAAAGAG / IVS10-43 / RG5-R11 / GACAGTCATCATCAGTTGACCCTTG / IVS11+71
RG5-F12 / CGCATAAAGACGTACCCTTTCCAG / IVS11-84 / RG5-R12 / GGGAAATCGCTTACTTAGAGCAGC / IVS12+54
RG5-F13 / GGGACTAGGACATTGACAGGAGG / IVS12-258 / RG5-R13 / GCCTGCCCATTAACCCTTTCC / c2059as
RG5U-F1 / AGATGGCAGGCAGGAAAGGG / c2027s / RG5U-R1 / CCAGTTCAGTCACAATGTCCATGC / c2220as
RG5U-F2 / TGTGACTGAACTGGTCCAATGATG / c2207s / RG5U-R2 / GAGGGGTGGTAAGCAGCAGTG / c2457as
RG5-1F / AGGCTCAGTTACAGGCTCAGAGG / c145s / RG5-1R / ATGATTTGATGTTCCACCAGGG / c254as
RG5-2F / CCTTCAGCGTCAGCAACCG / c198s / RG5-2R / GCCGTTCACAAACACTTCCCC / c418as
RG5-3F / CTTGGAAGGGGAAGTGTTTGTG / c391s / RG5-3R / GCATCGCCGTGTATCTCAGC / c527as
RG5-4F / GCTGAGATACACGGCGATGC / c508s / RG5-4R / AGTTGGGCTGCGATGGACAC / c687as
RG5-5F / TTGGGGGGATTTCCAGTGGC / c636s / RG5-5R / CAGTCCTGTGGTTGGCTCGTC / c736as
RG5-6F / TGATGCTTGACGAGCCAACC / c708s / RG5-6R / GAATGTTCAGGACAGGGGTAACC / c951as
RG5-7F / TGACTTACGGAGAGTTGGTGTTCTG / c864s / RG5-7R / GGGTAGGGTTTTCAGGTGTCTTG / c1123as
RG5-8F / TGGACACCCAAAGCAGAGAGC / c987s / RG5-8R / TGGACTCGGAGAAGGTAGAAAATG / c1290as
RG5-9F / GCAGGTGGTGATTATGCGTCTTG / c1216s / RG5-9R / GCAACGATGCTGAAGGGGAG / c1500as
RG5-10F / TCAGCGACCAGGAGAGTCAGG / c1410s / RG5-10R / CCATACCAAGCAGCACAAGTGTC / c1637as
RG5-11F / GACTCTGGGCTTGTATCCCGAG / c1531s / RG5-11R / CAGGCCATAGAACTCATTGACCAC / c1816as
RG5-12F / TGGTCAATGAGTTCTATGGCCTG / c1794 / RG5-12R / GACAAGAGTCGGGATGAACGAG / c1972as
RG5c-F / GAGGATTCACTCACATTTGCTTCC / c33s / RG5c-R / GCACCACACTGCCCCATAAG / c2198as
/

Abcg8

RG8-F2 / CATGATCCACTTCCTGTCAGTGTG / IVS1-114 / RG8-R2 / CCCACCCCTGAACATTCTATTACC / IVS2+64
RG8-F3 / CCTCTGAATGGCTCAGCTTCC / IVS2-48 / RG8-R3 / CATCCATGACTGAAGGCTGTTCTG / IVS3+71
RG8-F4 / GAGGGGAAGTAGTTGGCAGGTAAG / IVS3-67 / RG8-R5 / AGGTTGTAGGCTCAGGTCCTCG / IVS5+45
RG8-F6 / TCACTGGGCTTCCCTGTGCG / IVS5-31 / RG8-R6 / TGTGTCCAGGCTCCAGGTCG / IVS6+38
RG8-F7 / GATCAGGCAGAGTCAGGGACTAAC / IVS6-129 / RG8-R9 / GAGCGAAGAGTGTTACTGAGGTCG / IVS9+135
RG8-F10 / CCCACGGCATTACAAGAGATGC / IVS9-72 / RG8-R10 / GACTGCATGGCTGAGTGTTTCC / IVS10+83
RG8-F11 / AGCAAGCACTGGTGTCGGC / IVS10-50 / RG8-R11 / CCTGGCTAAGCAAAGAGGGG / IVS11+31
RG8-F12 / GACTCTTCTCTCCAGGAGCAGTCC / IVS11-93 / RG8-R13 / TCATTGTCCTCAGTCGGGCTC / c2207as
RG8U-F1 / TTCTGCCTGGGCTGGCCGCCTC / c2160s / RG8U-R1 / CATCACTCCCAGCCTCAAACAC / c3082as
RG8U-F2 / AGTGTGTGGCTACTGTCGTTTCTTC / c2650s / RG8U-R2 / GGTGGGTAAGGAATGAGCAAGG / c3717as
RG8U-F3 / GCTCTGTTGCCACCATCCATTAC / c3312s / RG8U-R3 / GCTCTGGGGACAAACTGAAGAAC / c4420as
RG8-1F / GCTGAGAAGACCAAAGAGGAGAC / c114s / RG8-1R / GACCACTGTAGGTGAAGTAGAGGCTG / c237as
RG8-2F / CAACAGCCTCTACTTCACCTACAGTG / c209s / RG8-2R / GCGAGACCTCCACGGTAACTTG / c344as
RG8-3F / TCAAGTTACCGTGGAGGTCTCG / c322s / RG8-3R / GGGCGATGAAAGTCAGGGTC / c624as
RG8-4F / GCTGCTCCCCAATCTGACTGTC / c578s / RG8-4R / GCACCCCGATGCTCACTCTT / c786as
RG8-5F / AAGAGTGAGCATCGGGGTGC / c767s / RG8-5R / CCAGGCGGGACAAAGTTCTC / c885as
RG8-6F / CAACCTGGTGAGAACTTTGTCCC / c857s / RG8-6R / CGCCTGTCAATGCTCGTCAAG / c1099as
RG8-7F / TGACGAGCATTGACAGGCG / c1081s / RG8-7R / CAGCAGCAGTTCCACAGTTGG / c1278as
RG8-8F / CCAACTGTGGAACTGCTGCTGAG / c1258s / RG8-8R / TGAAGGAGAGCGGCTTGTCTG / c1443as
RG8-9F / CGTCAGATTTCCAATGACTTCCG / c1320s / RG8-9R / CGTCCTCCAGTTCATAGTACAGCAG / c1560as
RG8-10F / CGGTCGCTGCTGTACTATGAACTG / c1530s / RG8-10R / CACCAGCCACAGAAGCATGAAG / c1721as
RG8-11F / GTGCCTATGTCATCATCTATGGGATG / c1621s / RG8-11R / CGATCTGCGTGGTGTAAATGTGTC / c1959as
RG8-12F / TGGACACATTTACACCACGCAG / c1934s / RG8-12R / AATACAGGGACAGGAAGCCACAG / c2082as
RG8c-F / AGGACTCAAGTGCCCTAGCTCTAAG / c4s / RG8c-R / CAGTTTTAGGTCACCCTCTGTATCG / c2545as

a Numbering based on Genbank No. NM_053754 (Abcg5) and Genbank No. NM_130414 (Abcg8)

b Position in intron 1 of Abcg8