Supplemental Table S3. Experimental Design Used for Microarray Analyses

Supplemental Table S3. Experimental design used for microarray analyses.

Comparison / Bulked cultivars/lines / Presence/ absence of QTL Fhb1a / Treatmentb / Experiment ID / Biological replicate (Extract ID) / Label / Hybridization ID
A
(Microarray comparison I) / 4 (E1-97U, E2-2U, E2-101U, CM82036) / Present / Deoxynivalenol (DON) / A / 1 / Cy3 / 1
4 (E2-51U, E1-1U, D2-60T, E2-77U) / Absent / DON / A / 1 / Cy5 / 1
4 (E1-97U, E2-2U, E2-101U, CM82036) / Present / DON / A / 2 / Cy5 / 2
4 (E2-51U, E1-1U, D2-60T, E2-77U) / Absent / DON / A / 2 / Cy3 / 2
4 (E1-97U, E2-2U, E2-101U, CM82036) / Present / DON / B / 1 / Cy3 / 3
4 (E2-51U, E1-1U, D2-60T, E2-77U) / Absent / DON / B / 1 / Cy5 / 3
4 (E1-97U, E2-2U, E2-101U, CM82036) / Present / DON / B / 2 / Cy5 / 4
4 (E2-51U, E1-1U, D2-60T, E2-77U) / Absent / DON / B / 2 / Cy3 / 4
B
(Microarray comparison II) / 4 (E2-28T, E2-138T, E2-1T, E1-80U) / Present / DON / A / 1 / Cy3 / 5
4 (E2-51U, E1-1U, D2-60T, E2-77U) / Absent / DON / A / 1 / Cy5 / 5
4 (E2-28T, E2-138T, E2-1T, E1-80U) / Present / DON / A / 2 / Cy5 / 6
4 (E2-51U, E1-1U, D2-60T, E2-77U) / Absent / DON / A / 2 / Cy3 / 6
4 (E2-28T, E2-138T, E2-1T, E1-80U) / Present / DON / B / 1 / Cy3 / 7
4 (E2-51U, E1-1U, D2-60T, E2-77U) / Absent / DON / B / 1 / Cy5 / 7
4 (E2-28T, E2-138T, E2-1T, E1-80U) / Present / DON / B / 2 / Cy5 / 8
4 (E2-51U, E1-1U, D2-60T, E2-77U) / Absent / DON / B / 2 / Cy3 / 8

aInherited from wheat cv. CM82036.

bDON: 5g ml-1 0.2% Tween20; harvest = 4h post-treatment.