Bio-Process Lab
BIO-PROCESS LAB
SAMPLE TOURNAMENT #3
Station A: Microscopy
1. List the powers of the objectives for this microscope.
2. List the range of magnification (lowest to highest) for this microscope.
Place the transparent ruler on the stage, hold it down with the stage
clips and focus on the metric scale with the scanning (5X) power objective.
Hint: Applying gentle pressure to the free end of the
ruler will help to adjust for the thickness of the ruler and allow
better focus.
3. What is the diameter of the scanning (5X) field of this microscope in millimeters ?
in micrometers?
4. Assuming this photo of hydra (below) was taken using the scanning power field of
this microscope, what is the length in micrometers of the bud in mm? in mcm?
5. A chain of spirogyra is observed under low power (10X) of this microscope. A student
counts 6 cells along the diameter of the field of view. How many cells should be
visible along the scanning power (5X) field of view?
Materials: Microscope with 10X ocular and 5X, 10X, 40X and 100X objectives, clear mm ruler,
hydra photo.
Station B: Lab Safety
Examine the safety symbols and list of activities provided and answer the following questions.
For questions 6 & 7 , use the safety symbols
6. Symbol “A” represents what type of hazard?
7. Symbol “B” represents what type of hazard?
For questions 8 & 9, use the list of observed activities
8. Which of the observed activities would be considered safe and proper for a student’s
health and safety?
9. Which of the observed activities would be considered unsafe and should not be done in
the laboratory?
10. For the situation described below, explain the correct procedure for dealing with the emergency.
You break a test tube and cut your finger.
SAFETY SYMBOLSLIST OF OBSERVED ACTIVITIES
A B A. Using the eye wash station for a drinking fountain.
B. Wearing safety goggles while dissecting a fetal pig.
C. Placing broken glassware into a designated container.
D. Using the scalpel from your dissecting kit to cut up
your candy bar.
E. Using Pyrex glassware when heating liquids.
Station C: Predictions
Examine the lab setup for the germination of corn seeds.
Use diagrams to show your answers for questions 11 & 12.
11. How will the shoot and root grow for seed “B” ?
12. How will the shoot and root grow for seed “D” ?.
13. What type of geotropism will the corn roots exhibit? (positive or negative).
14. What type of phototropism will the corn shoots exhibit? (positive or negative)
15. How might the germination of these seeds be affected if the experiment were conducted
in outer space? Why?
DIAGRAM OF LAB SETUP FOR CORN SEEDS
Station D: Lab Equipment
Use the list of equipment provided to answer the following questions
16. Give the letter of the piece of equipment that should be used to measure 0.1 mL of a liquid.
17. Give the letters of the pieces of equipment that should be used to dissect a shark.
18. Give the letters of the pieces of equipment that should be used to make a wet mount?
19. Give the letters of the pieces of equipment that should be used to heat 10 mL of a liquid.
20. Give the letters of the pieces of equipment that should be used to observe a planarian feeding.
LIST OF EQUIPMENT
A. dissecting stereoscopeI. coverslip
B. dissecting pan J. compound microscope
C. safety goggles K. small culture dish
D. dissecting kit L. test tube holder
E. Pyrex test tube M. eye dropper
F. 1 mL pipetN. Bunsen burner
G. microscope slideO. mortar & pestle
H. 10 mL graduated cylinderP. 10 mL pipet
Station E: Measurement
Using the instruments provided, obtain the requested information.
21. Measure the length of the critter from A to B.
What is it's length in millimeters? in centimeters?
22. What is the value of the numbered and unnumbered increments or
graduations of the thermometer.
23. What is the temperature recorded on the thermometer?
24. What is the value of the numbered and unnumbered increments or
graduations of the graduated cylinder?
25. What is the volume present in the graduated cylinder?
Station F: Punnett Square Analysis
Examine the Punnett Square below and answer the questions.
Background: Genotype is the gene combination and phenotype is the appearance of the trait.
Capital letters indicate dominant alleles and lower case letters indicate recessive alleles.
In race horses, black hair (B) is dominant over chestnut hair (b) and a trotting gait (T) is
dominant over a pacing gait (t). Tow heterozygous black trotters are mated (BbTt X Bb Tt).
26. How many of the genotypes in the Punnett Square will produce a phenotype
with black hair?
27. How many of the genotypes in the Punnett Square will produce a phenotype
with a pacing gait?
28. How many of the genotypes in the Punnett Square will produce a phenotype
with chestnut hair and a trotting gait?
29. How many of the genotypes in the Punnett Square will produce a phenotype
with chestnut hair and a pacing gait?
30.
Station G: Evolution
Use the diagrams of forearm (ulna & radius) structures to answer the following questions.
31. Which animal has the ulna and radius long, thin and light in weight? What is the function
of this limb?
32. Which animal has both the ulna and radius short and thick? What is the function of this
limb?
33. Which animal has the ulna long and straight with the radius small and straight? What is
the function the this limb?
34. These animals have similar anatomical structures modified for different structures. What
term describes this situation?
35. Birds and insects have wings for flight, but they are anatomically different. What term
describes this situation?
DIAGRAMS OF FOREARM OF THREE MAMMALS
dolphin horsebat
Station H: Experimental Design & Analysis
Examine the experimental setup and answer the following questions.
36. Which jar is control for all other jars in this experiment? What does it contain?
37. Which indicator can be used to show the carbon dioxide concentration of the water?
A. iodine B. methyl red C. bromthymol blue D. glucose test-strip
38. Which tubes will have oxygen produced when the jar is placed in the light?
39. Which organisms will use oxygen and produce carbon dioxide inside the jars?
40. If the jars are sealed and illuminated, in which jar would the fish live the longest? Why?
LAB SETUP USING FISH AND ELODEA
Note: all jars contain pond water and are sealed as well as being placed in the light..
Station I: Inferences
Inferences are logical conclusions based upon observations
Visually examine, but do not touch the potted flowering geranium.
Answer the following questions using this key.
A. Both are observations.
B. Both are inferences.
C. The 1st is an observation; the second is an inference.
D. The 1st is an inference; the second is an observation.
41. The plant has leaves. The plant has roots.
42. The plant is absorbing water. Water is absorbed by the roots.
43. The plant has flowers. The stem is green.
44. Transpiration takes place in the leaves. The leaves have net veination.
45. The soil is moist. The roots are filling the pot below the soil.
Materials: a potted flowering geranium with roots below the soil.
Station J: Ecology -- Food Web
Examine the food web of a pond and answer the following questions.
46. What are the producers for this pond ecosystem?
47. Which organisms eat only the producers in this pond ecosystem?
48. What are the highest order consumers for this ecosystem?
49. How many food chains are present in this food web?
50. What are the decomposers listed in this food web?
ANSWER KEY:SAMPLE TOURNAMENT #3 SCHOOL NUMBER .
BIO-PROCESS LAB SCHOOL .
STATE .
STUDENT NAMES: (PLEASE PRINT) RAW SCORE .
1. RANK .
2. POINTS .
BE SURE TO INCLUDE APPROPRIATE UNITS WITH ALL ANSWERS!!!
STATION A: STATION F:
1. 5X 10X 40X 100X 26. 12 .
2. 50X to 1000X 27. 4 .
3. ~ 3.0 mm ~ 3000 mcm 28. 3 .
4. ~ 1.0 mm ~ 1000 mcm 29. 1 .
5. 12 cells 30. D. – 9:3:3:1 .
STATION B: STATION G:
6. electrical safety 31. bat - flying .
7. poison safety 32. dolphin - swimming .
8. B,C,E 33. horse - walking .
9. A,D 34. homologous .
10. Tell instructor & obtain 35. analogous .
approved first aide
STATION C: STATION H:
11. see diagram 36. # 1 - pond water .
12. see diagram 37. C (bromthymol blue) .
13. positive 38. # 4 and # 5 .
14. positive 39. fish and Elodea .
15. roots won’t grow - 40. #4- Elodea produces oxygen
not enough gravity and food for the fish
STATION D: STATION I:
16. F 41. C .
17. B,C,D 42. B .
18. G,I,M 43. A .
19. C,E,L,N 44. D .
20. A,K,M(opt) 45. C .
STATION E: STATION J:
21. 124.0 mm 12.40 cm 46. pond plants & plankton .
22. n = 10o C un = 1o C 47. snails & insects .
23. 63.5o C 48. turtles & ducks .
24. n = 5 mL un = 1 mL 49. 8 food chains .
25. 53.5 mL 50. none are listed on food web