Title: Genetic Diversity in Some Grape Genotypesvarieties Revealed by Scot Analyses

Title: Genetic diversity in some grape genotypesvarieties revealed by SCoT analyses

Journal: molecular biology reports

Author: Da-Long Guo·Jun-Yu Zhang·Chong-Huai Liu

Affiliation and e-mail address of the corresponding author: College of Forestry, Henan University of Science and Technology, Luoyang 471003, Henan Province, P.R. China Fax：+86-0379-64282345 e-mail:

Table S1 The grape varieties used in this study.

Code / Name / Pedigree / Species / Origin
1 / 87-1 / - / V.vinifera L. / China
2 / Fenghuang 51 / Muscat of Alexandria×Cardinal, uncertain / V.vinifera L. / China,1988
3 / Hetianhong / Ancient variety of China , Unknown / V.vinifera L. / China
4 / Heijixin / Ancient variety of China , Unknown / V.vinifera L. / China
5 / Hongjixin / Ancient variety of China , Unknown / V.vinifera L. / China
6 / Lizixiang / Ancient variety of China , Unknown / V.vinifera L. / China
7 / Longyan / Ancient variety of China , Unknown / V.vinifera L. / China
8 / Munage / Ancient variety of China , Unknown / V.vinifera L. / China
9 / Niunai / Ancient variety of China , Unknown / V.vinifera L. / China
10 / Pinger Putao / Ancient variety of China , Unknown / V.vinifera L. / China
11 / Xiabai / Ancient variety of China , Unknown / V.vinifera L. / China
12 / Niuxin / Ancient variety of China , Unknown / V.vinifera L. / China
13 / Baijixin / Ancient variety of China , Unknown / V.vinifera L. / China
14 / Zexiang / Muscat Hamburg×Longyan / V.vinifera L. / China,1979
15 / Zhengguodawuhe / Ancient variety of China , Unknown / V.vinifera L. / China
16 / Zhengzhouzaohong / Muscat Hamburg×Pearl of Csaba / V.vinifera L. / China,1962
17 / Zhengzhouzaoyu / Queen of vineyard×Italia / V.vinifera L. / China,1964
18 / Wuhezi / Unknown / V.vinifera L. / Unknown
19 / Jingkejing / Blue French×Newcastle / V.vinifera L. / China,1960
20 / Rizamat / Катта Курган × Паркет / V.vinifera L. / Unknown
21 / Autumn Royal / Autumn Black×C741 / V.vinifera L. / USA
22 / Thompson Seedless / Gold×Fzdi (Emperor×Pirovam75) / V.vinifera L. / USA,1981
23 / Queen of vineyard / Elisabeth×Pearl of Csaba / V.vinifera L. / Hungary,1916
24 / Pearl of Csaba / Mosknellier d’Hongrie×Nuscat Ottonel / V.vinifera L. / Hungary,1904
25 / Muscat of Alexandria / Unknown / V.vinifera L. / Egypt
26 / Italia / Bicane×Muscat Hamburg / V.vinifera L. / Italy,1911
27 / Cardinal / Flame Tokay×Ribier / V.vinifera L. / USA,1939
28 / Centennial Seedless / Gold×Q25～6 / V.vinifera L. / USA,1981
29 / Fenghou / Chance seedling of Kyoho / V.vinifera L.×V.larbrusca L. / China,1990
30 / Hojyu / Igawa 243×Canno Hall Muscat / V.vinifera L.×V.larbrusca L. / Japan,
31 / Benifuji / Gruaud-Larose×Kuroshio / V.vinifera L.×V.larbrusca L. / Japan,
32 / Benizuiho / Gruaud-Larose×Kuroshio / V.vinifera L.×V.larbrusca L. / Japan,1963
33 / Hongshuangwei / Queen of Vineyard×(Muscat Hamburg× TriumpH) / V.vinifera L.×V.larbrusca L. / China,1994
34 / Kyoho / Campbell E× Centenial / V.vinifera L.×V.larbrusca L. / Japan,1945
35 / 98-2 / Sport of Kyoho / V.vinifera L.×V.larbrusca L. / China
36 / Champbell Early / Moore Early×（Belvidere×Muscat Hamburg） / V.vinifera L.×V.larbrusca L. / Japan
37 / Gamay Blanc / - / V.vinifera L. / France
38 / Cabernet Sauvignon / Cabernet Fran× Sauvignon Blan / V.vinifera L. / France
39 / Merlot / Unknown / V.vinifera L. / France
40 / Roter Traminer / Unknown / V.vinifera L. / Greece
41 / Wanhongmi / Unknown / V.vinifera L. / Georgia
42 / Petit Verdot / Unknown / V.vinifera L. / France
43 / Yan 73 / Muscat Hamburg×Alicante Bouschet / V.vinifera L. / China,1966
44 / Yida / Unknown / V.vinifera L. / Hungary
45 / Yujinxiang / Unknown / V.vinifera L. / Unknown
46 / Beichun / Muscat Hamburg×V.amurensis / V.vinifera L. ×V.amurensis / China,1954
47 / Beimei / Muscat Hamburg×V.amurensis / V.vinifera L. ×V.amurensis / China,1965
48 / Xiongyuebaiputao / Muscat Hamburg× V.amurensis × Longyan / V.vinifera L. ×V.amurensis / China,1990
49 / SO4 / - / V..berlandieri× V.riparia / Germany
50 / Gloire / Wild specie / - / USA
51 / V.champini / Wild specie / V.champini Planch. / USA
52 / V.riparia / Wild specie / V.riparia Michx. / USA
53 / V.rupestris / Wild specie / V.rupestris Scheele. / USA
54 / Beaumout / - / USA
55 / Beta / Carvera×Concord / V.riparia×Concord / USA
56 / Tangweiputao / Wild species / V.davidii Foex. / China,
57 / V.heyneana / Wild species / V.heyneana Roem. & Schult. / China,
58 / V.davidii / Wild species / V.davidii Foex. / China,
59 / V.amurensis / Wild species / V.amurensis Rupr. / China
60 / V.pseudoreticulata / Wild species / V.pseudoreticulata W.T. Wang / China
61 / V.piasezkii / Wild species / V.piasezkii Maxim. / China
62 / V.flexuosa / Wild species / V. flexuosa Thunb. / China
63 / V.bellula / Wild species / V.bellula (Rehd.) W. T. Wang / China
64 / V.quinquangularis / Wild species / V. quinquangularis Rehd. / China

Table S2 Characterizations of 17 SCoT makers in 64 grape varieties.

Code No. / Primers(5'-3') / NAB / NPB / PPB％ / PIC / H / SI
SCoT3 / CAACAATGGCTACCACCG / 7 / 7 / 100 / 0.79 / 0.19 / 0.33
SCoT6 / CAACAATGGCTACCACGC / 9 / 5 / 55.6 / 0.81 / 0.11 / 0.19
SCoT12 / ACGACATGGCGACCAACG / 5 / 5 / 100 / 0.76 / 0.47 / 0.66
SCoT13 / ACGACATGGCGACCATCG / 9 / 9 / 100 / 0.86 / 0.40 / 0.58
SCoT14 / ACGACATGGCGACCACGC / 6 / 6 / 100 / 0.78 / 0.42 / 0.60
SCoT15 / ACGACATGGCGACCGCGA / 7 / 7 / 100 / 0.76 / 0.26 / 0.42
SCoT18 / ACCATGGCTACCACCGCC / 5 / 5 / 100 / 0.76 / 0.44 / 0.63
SCoT19 / ACCATGGCTACCACCGGC / 10 / 10 / 100 / 0.87 / 0.37 / 0.55
SCoT21 / ACGACATGGCGACCCACA / 11 / 9 / 81.8 / 0.90 / 0.33 / 0.48
SCoT22 / AACCATGGCTACCACCAC / 10 / 10 / 100 / 0.82 / 0.30 / 0.45
SCoT28 / CCATGGCTACCACCGCCA / 5 / 4 / 80.0 / 0.75 / 0.30 / 0.44
SCoT29 / CCATGGCTACCACCGGCC / 6 / 6 / 100 / 0.81 / 0.45 / 0.65
SCoT31 / CCATGGCTACCACCGCCT / 9 / 9 / 100 / 0.86 / 0.40 / 0.58
SCoT33 / CCATGGCTACCACCGCAG / 8 / 8 / 100 / 0.85 / 0.43 / 0.62
SCoT34 / ACCATGGCTACCACCGCA / 6 / 6 / 100 / 0.80 / 0.35 / 0.54
SCoT35 / CATGGCTACCACCGGCCC / 12 / 10 / 83.3 / 0.91 / 0.38 / 0.54
SCoT36 / GCAACAATGGCTACCACC / 6 / 6 / 100 / 0.76 / 0.29 / 0.45
Average / - / 7.706 / 7.176 / 94.16 / 0.82 / 0.35 / 0.51

NAB = Number of amplified bands; NPB = number of polymorphic bands; PPB = percent of polymorphicbands; PIC= polymorphism informationcontent;H =Nei’ genetic diversity; SI=Shannon’s information index.