Table S1 the Property of Sea Water at Sampling Site

Supplementary data

Table S1 The property of sea water at sampling site

Cu(II)
(mg/L) / DO
(mg/L) / pH / ORP
(mV) / Temperature
(℃) / Conductivity (us/cm) / Salinity
(‰) / Total nitrogen
(mg/L)
Sea water / 6.85 / 7.12 / 7.80 / -60 / 18.1 / 18 / 31.2 / 1.405

Table S2 The sediment characteristics of sampling site

Cu(II)
(mg/kg) / Total phosphorus
(mg/kg) / Total nitrogen
(mg/kg) / Organic nitrogen
(N%) / Organic carbon
(C%) / Organic matter
(%)
Sediment / 32.73 / 522.06 / 1780 / 0.18 / 1.02 / 5.10

Table S3Closest phylogenetic affiliations of partial 16SrDNA sequences from the bands excised from DGGE gela

Band No. / Organism, Accession No. / Similarity
(%) / Phylogenetic affiliation / Isolation source
1-1 / Thioalkalivibrio sp. ALMg14, EU709869 / 100 / γ-Proteobacteria / Soda lake sediment
1-2 / Tetrasphaeraaustraliensis (T), AF125091 / 92 / Actinobacteria / Activated sludge
2-1 / Rubrobacterxylanophilus(T),X87135 / 94 / Actinobacteria / Thermally polluted industrial runoff
2-2 / Nitrospira enrichment culture Ns4a, HM485590 / 93 / Nitrospira / Kamchatka hot spring
3-1 / Bilophilawadsworthia,AB117562 / 95 / δ-Proteobacteria / Human feces
3-2 / Magnetobacteriumbavaricum,X71838 / 96 / δ-Proteobacteria / LakeChiemsee freshwater sediment
4-1 / Alpha- proteobacteriumHTB015, AB010864 / 97 / α-Proteobacteria / South Japan deep marine sediment
4-2 / Acidiphilium sp. DX1-13, EF556231 / 96 / α-Proteobacteria / -b
5-1 / Thiobacillusprosperus, EU653290 / 98 / γ-Proteobacteria / Acidic, marine sediments
5-2 / Desulfocapsasulfexigens(T), Y13672 / 92 / δ-Proteobacteria / Arcachon marine surface sediment , France
6-1 / Alpha - proteobacteriumPWB3, AB106120 / 96 / α-Proteobacteria / Kamaishi Bay sea water
6-2 / MagnetospirathiophilastrainMMS-1, EU861390 / 95 / α-Proteobacteria / Salt marsh in Woods Hole, Massachusetts
7-1 / Desulfohalobiumretbaense DSM 5692, CP001734 / 88 / δ-Proteobacteria / Sediments of the hypersaline Retba Lake, Senegal
7-2 / Desulfomicrobiumthermophilum (T), AY464939 / 89 / δ-Proteobacteria / Terrestrial hot spring in Colombia
8-1 / Saccharospirillum impatiens(T),AJ315983 / 91 / γ-Proteobacteria / HypersalineEkhoLake, East Antarctica
8-2 / Pelobacter sp. A3b, AJ271656 / 96 / δ-Proteobacteria / Black Sea shelf sediments
9-1 / Olaviusloisae endosymbiont 2, AF104473 / 95 / α-Proteobacteria / Gutless marine oligocheteOlaviusloisae, Annelida
9-2 / Alkalilimnicolaehrlichii(T), AF406554 / 99 / γ-Proteobacteria / Mono Lake, California
10-1 / Dehalogenimonas lykanthroporepellens BL-DC-9, EU679419 / 94 / Chloroflexi / Chlorinated solvent-contaminated groundwater near Baton Rouge, LA, USA
10-2 / Defluvibacterlusatiensis (T), AJ132378 / 100 / α-Proteobacteria / Activated sludge
11-1 / Oceanibaculumindicum(T), EU656113 / 98 / α-Proteobacteria / Deep seawater of the Indian Ocean
11-2 / Uncultured Nitrospirae bacterium, EU780676 / 99 / δ--Proteobacteria / LakeMiyun,Northern China
12-1 / Novosphingobium sp. MG35, AJ746092 / 97 / α-Proteobacteria / Haemodialysis water and fluid
12-2 / Uncultured bacteriumCM115, EF580967 / 94 / Acidobacteria / Freshwater Calcareous Mats
13-1 / Desulfocurvusvexinensis(T), DQ841177 / 86 / δ--Proteobacteria / Deep subsurface aquifer
13-2 / Desulfocurvusvexinensis (T), DQ841177 / 89 / δ--Proteobacteria / Deep subsurface aquifer
14-1 / Tepidamorphusgemmatus, GU187912 / 97 / α-Proteobacteria / Hot spring inSão Miguel, Azores
14-2 / Bacteriovoraxsp. DB1, EF092438 / 91 / δ--Proteobacteria / Seawater shrimp ponds, adjacent coastal waters
15-1 / Desulfocurvusvexinensis (T), DQ841177 / 89 / δ--Proteobacteria / Deep subsurface aquifer
15-2 / Sulfate-reducing bacterium BKA11, AJ012597 / 87 / δ--Proteobacteria / Rice field soil
16-1 / Dehalococcoides sp. BHI80-52, AJ431247 / 91 / Chloroflexi / East Pacific Rise
16-2 / Dehalococcoides sp. BHI80-15,AJ431246 / 90 / Chloroflexi / East Pacific Rise
17-1 / Uncultured delta proteobacterium, GQ850566 / 99 / δ--Proteobacteria / Northern Bering Sea
17-2 / Sulfate-reducing bacterium EZ-2C2,AJ012598 / 90 / δ--Proteobacteria / Rice field soil
18-1 / Hirschiabaltica,X52909 / 94 / α-Proteobacteria / Phycosphere of the toxin-producing dinoflagellate Prorocentrumlima
18-2 / Amaricoccusveronensis (T), U88043 / 97 / α-Proteobacteria / Activated sludge biomass
19-1 / Clostridium bifermentans, DQ680018 / 98 / Firmicutes / Sugarcane crop soil
19-2 / Fodinicurvata fenggangensis (T), FJ357427 / 96 / α-Proteobacteria / Salt mine in Yunnan, Southwest China
20-1 / Amaricoccusveronensis (T), U88043 / 97 / α-Proteobacteria / Activated sludge biomass
20-2 / Albidovulumsp. S1K1, FJ222605 / 97 / α-Proteobacteria / Seafloor hydrothermal chimney
21-1 / Tepidamorphus gemmatusCB-27, GU187912 / 97 / α-Proteobacteria / Hot spring inSão Miguel, Azores
21-2 / Rhodococcus rhodochrous (T) DSM 43241, X79288 / 95 / Actinobacteria / - b
22-1 / Uncultured bacterium TopBa68, FJ748800 / 99 / δ--Proteobacteria / Pearl River Estuary sediments, Guangdong, China
22-2 / Uncultured bacteriumELSC-TVG13-B68, GU220761 / 99 / Planctomycetes / Low temperature hydrothermal precipitates, EastLauSpreadingCenter
23-1 / Rhodovibrio sodomensis strain SG3105, AJ318524 / 98 / α-Proteobacteria / Mediterranean salterns
23-2 / Rhodovibrio sodomensis strain SG3105, AJ318524 / 98 / α-Proteobacteria / Mediterranean salterns
24-1 / Rhodovibrio sodomensis strain SG3105, AJ318524 / 98 / α-Proteobacteria / Mediterranean salterns
24-2 / Uncultured bacterium MSB-1C5, EF125397 / 92 / δ--Proteobacteria / Naked tidal flat sediment near mangrove
25-1 / Uncultured bacterium P9X2b8D07, EU491240 / 99 / δ--Proteobacteria / Seafloor lavas from the Loi'hi Seamount Pisces Peak X2
25-2 / Anaerostipessp. AIP 183.04, AY833660 / 91 / Firmicutes / Human blood culture
26-1 / Schlegelella aquatica (T)wcf1, DQ417336 / 91 / β-Proteobacteria / Hot spring
26-2 / Rhodobacter sp. 2002-65602, AY244771 / 96 / α-Proteobacteria / Hlood culture
27-1 / Microbulbifer sp. GB02-4, GQ118701 / 96 / γ-Proteobacteria / GuaymasBasin deep-sea hydrothermal plume
27-2 / FlexithrixdorotheaeIFO 15974, AB078038 / 95 / Bacteroidetes / Brown sand

aTwosequences of each excised band were compared to nucleotide sequences in GenBank. The closest phylogenetic affiliation for the sequence is showed by the strain name, GenBank accession number,similarity, phylum or class, and theisolating source. Refer to Fig.2 for band positions.

btheisolating source not known.