Table S1. First and Nested PCR Primers for RACE-PCR
Table S1. First and nested PCR primers for RACE-PCR
Primer name / 5’ RACE reverse primer ( 5’ to 3’) / 3’ RACE forward primer ( 5’ to 3’)ncTLR1 / ATA GACTCAGCTTTC CAT GGCAGGTCT C / GTGCTGGTGCTGCTG GAG CCT CTA CCT C
ncTLR1 nested / CCATCAACATGGCTGCCGCCC AGA GAG C / CGGCACACATACCTG GAG TGGCCCCAG G
ncTLR2 / AGTCAGCGGGAC AAA TGCTTG CTC CTG G / CTC AGA ACT TCATCAGGAGTGAATGGT G
ncTLR2nested / GCTTTGATG CAT CAGCAGAAGGACACA C / GCTGCG GAG AGT CAT GAA CTC CAGGAC G
ncTLR3 / CAG AGA AGGGCT CTC CTTGTAGCTTTA G / TAT CGCCCAACATGCAATTCACCGGGA C
ncTLR3nested / CCGCAC TGA GGC TGA GGTGGCTGCAGT C / TCCGCAGGGGCATGTTGCGTC CAT GCT G
ncTLR5S / AGA GAT GTGGTT CAT CTC CAGGAACAG G / CACATCAGTAGTGGA GAT GGATCT CTC C
ncTLR5Snested / TTAGCTACA GAG CCA ATA ATGAGG CAT G / ACA AGA CCAATG TGA CCTTCCTCAGTC C
ncTLR5 / ACT GCTGTT GAT CTC ACT GAT GAAGTT C / CAT CAGGGACGCAATCTG GAG CAGCAG G
ncTLR5nested / CACAAG GCC GTG AAA AGTGCA TGA TGG G / AGC TGA TGAAGT ACC ACGCAGTCA GAG C
ncTLR7 / GTT GAT GGT GAG CGT CAG ATT GGT CGT G / TGA CGT TAT TGT ACT GAT CCTCTTGGA G
ncTLR7nested / GAAACG CTA GCTTTCAGTATGATGGGG C / CGCTCGGTGCTG GAG TGGCCC ACT AAC C
ncTLR8 / AAT GAG TCATTCCAA ATA TTACAAATG G / TTC AAA CTGGCAATG TAT CTG GCC CAC C
ncTLR8nested / GCTTTCAGCGGGCTG GAT CTTACATTG G / GCTGGA GCC CGTCCTGCAGCA CTC TCA C
ncTLR9 / TTTTGCCCC ACT TTCCGTATCTTAGTT C / TGG TGA AACGGTGAACGGTGT GAT TCG C
ncTLR9nested / CAA TGG AAG AAG TTG AAA AAT GAT GAA G / TTTCTGGAACCGAAT GAG AATGGC ACT G
ncTLR14 / GCC CCTGTAATCACACCGCTTGTT AAA C / TTCTGGGCACAGCTGAGGAGCGTTCTG G
ncTLR14nested / AGCTCCAGTCAAATCTCAGGA TAT CAG G / CAA AGA AGCAGT ACC GAA AGA TGA GAT C
ncTLR21 / TGCTCAAGTTTTTGG AGA TGA CCAAAG C / GAT GAG CACCGGGACGTC CTC CTGCTT G
ncTLR21nested / TTGAACTGC ACC AAGAAC AAA TGCAAT G / CTTATCTGCAGTGGC CTA GCTCAG ACT G
ncTLR22 / CGA TGA AGGAGC CAT TTGCAAGTTTCT C / TAACCTTTCGTATTTCAATGCAAGGAA C
ncTLR22 nested / CTT GAG GGATTGCAA GCC TTT AAA ATC C / TGA CCT AAA AGA TCTTTCTCCGGATCT G
ncTLR23 / ACA AAA TGTTCAGCTTTGACAAGTTTC C / CTC CTCACATCGTTCATCTACCACTTT C
ncTLR23nested / AATCCA AAA CAC CAT CTGCCA TAT ATT C / TAG CTTACACCTTCC ACC TCTTCCTCG C