Supplementary table 1: Provisional list of studies collaborating in the CRP-Coronary Disease Genetics Collaboration as of April 2008

Location / Study design / CHD cases / Controls / CRP levels / CRP genotypes measured
Study / Approx.
number / Definition / Ascertainment method / Sampling framework / Age range (years) / Approx. % male / Approx.
number / Sampling framework / Matching variables / Assay methods (source) / Assay standard
Prospective studies
Women’s Health Initiative / USA / Nested case- control / 2500 / MI or CHD death / Symptoms, cardiac enzymes, ECG / Mass mailing, media advertising and community presentations / 50-79 / 0 / 2500 / “Nested” within same prospective study / Age, smoking status, ethnicity / ITA (Denka Seiken) / Denka Seiken / GD
EPIC-Norfolk / England / Nested case- control / 2200 / ICD-8 410-414 / Death certification and hospital admission records / General practices in Norfolk / 40-78 / 64 / 2200 / “Nested” within same prospective study / Age and sex / ELISA (Sanquin Res) / Behringwerke / rs1130864, rs1205, rs3093077
Health in Men Study / Australia / Cohort / 1500 / Non-fatal MI or CHD death / Symptoms, hospital admissions records and death certification / Sample of AAA screening RCT / 70-88 / 100 / 2400 / Sample of AAA screening RCT / Age and sex / Dade Behring / Manufacturer / rs1205, rs1130864ii
Malmö Diet & Cancer Study / Sweden / Nested case- control / 1500 / First time (ICD-8)
non-fatal: 410
fatal: 410-414 / National hospital discharge and death registers / Population registers of Malmo area / 45-73 / 73 / 1500 / “Nested” within same prospective study / Age, sex, date of baseline exam. / – / – / GDiii
EPIC-Denmark* / Denmark / Nested case- cohort / 1200 / ICD-8 410 / National hospital discharge and death registers / Population registers of Copenhagen and Aarhus areas / 50-64 / 45 / 1800 / “Nested” within same prospective study / Age, sex, area / – / – / GD
Copenhagen Ischaemic Heart Disease Study / Denmark / Cohort / 1150 / ICD-8 410 / Symptoms, cardiac enzymes, ECG, coronary angiography / CopenhagenUniversityHospital / 20-90 / 72 / 8000 / Copenhagen population register / Age and sex / Dade BehringDiagnostica / – / rs1130864, rs1205, rs3093077
Copenhagen General Population Study / Denmark / Cohort / 1000 / ICD-8 410 / National hospital discharge and death registers, follow-up examinations / Copenhagen population register / 20-90 / 45 / 28 000 / Copenhagen population register / Not matched / Dade BehringDiagnostica / – / rs1130864, rs1205, rs3093077
British Women’s Heart and Health Study / UK / Nested case-control / 986 / Non-fatal MI or CHD death (ICD10 I20-I25, I51.6) / Medical records, self-reported history, death certification / GP/hospital / 60-79 / 0 / 2563 / GP/Hospital / Multiple / INA (Behring) / Manufacturer / rs1130864, rs1205vi
CopenhagenCity Heart Study / Denmark / Cohort / 800 / ICD-8 410 / National hospital discharge and death registers, follow-up examinations / Copenhagen population register / 20-90 / 46 / 9000 / Copenhagen population register / Not matched / Dade BehringDiagnostica / – / rs1130864, rs1205, rs3093077
PROSPER / UK, Netherlands / Nested case-control / 640 / Non-fatal MI or CHD death / ECG, medical records / 70-82 / 48 / 5042 / Not matched / ITA (Roche) / cFAS calibrator / rs1205viii
Northern Swedish Cohorts / Sweden / Nested case-control / 589 / First time fatal and non-fatal MI / Symptoms, cardiac enzymes, ECG / MONICA Northern Sweden / 29-76 / 73 / 1093 / “Nested” within same prospective study / Age, sex, residential area / CIA (Immulite) / WHO 85/506 / rs1130864, rs1205, rs3093077ix
Cardiovascular Health Study / USA / Cohort / 528 / ICD-9 410-414, 427 / ECG, enzymes, symptoms and death certification / Field centres / ≥65 / 42 / 4641 / ELISA
(in house) / WHO 85/506 / rs1205x
English Longitudinal Study of Aging / UK / Cohort / 500 / MI or CHD death / Symptoms, hospital admissions records and death certification / Sample of the Health survey for England / >50 / 4500 / Sample of the Health survey for England / Not matched / INA (Dade Behring) / Manufacturer / GD
WOSCOPS / Scotland / Nested case-control / 500 / Non-fatal MI or CHD death / Symptoms, cardiac enzymes, ECG, medical records / Heart screening clinic / 45-65 / 100 / 1100 / West of Scotland / Age / ELISA
(in-house) / IFCC CRM470 / rs1130864, rs1205, rs3093077
Caerphilly Cohort / UK / Nested case-control / 492 / Non-fatal MI or CHD death (ICD10 I20-I25, I51.6) / ECG, medical record, self-reported history and death certification / GP/hospital / 50-80 / 100 / 442 / Multiple / INA (Dade Behring) / Manufacturer / rs1130864, rs1205xi
British Regional Heart Study / UK / Cohort / 480 / MI or CHD death / ECG, enzymes, symptoms and death certification / GP registers / 60-79 / 100 / 4252 / GP registers / Not matched / MEIA (Abbott);
INA (Dade Behring) / WHO 85/506; Manufacturer / GD
Whitehall I / UK / Cohort / 446 / Non-fatal MI, ICD-9 410-414 / Symptoms, ECG and treatment history / British civil servants / 40-64 / 100 / 3400 / British civil servants / Not matched / TF† / TF† / rs1130864, rs1205, rs3093077
Rotterdam Study / Netherlands / Cohort / 388 / MI or CHD death / ECG, enzymes, symptoms and death certification / Random sample Ommoord suburb (Rotterdam) / >55 / 40 / 4843 / Random sample Ommoord suburb (Rotterdam) / Not matched / INA (Immage) / Manufacturer / rs1130864, rs1205xii
Health Professionals Follow-Up Study / USA / Nested case-control / 265 / Non-fatal MI or CHD / ECG, symptoms, enzyme levels, medical records and death certification / Registered health professionals, 1986 / 40-75 / 100 / 529 / Registered health professionals, 1986 / Age, smoking status, date of blood sampling / ITA (Denka Seiken) / Denka Seiken / rs1130864, rs1205xiii
Location / Study design / CHD cases / Controls / CRP levels / CRP genotypes measured
Study / Approx.
number / Definition / Ascertainment method / Sampling framework / Age range (years) / Approx. % male / Approx.
number / Sampling framework / Matching variables / Assay methods (source) / Assay standard
Edinburgh Artery Study / Scotland / Cohort / 260 / Non-fatal MI or CHD death / ECG, medical record and death certification / GP / 55-74 / 50 / 1300 / GP / Not matched / INA (Behring) / Manufacturer / rs1130864, rs1205, rs3093077
Nurses’ Health Study / USA / Nested case-control / 239 / Non-fatal MI or CHD / ECG, symptoms, enzyme levels, medical records and death certification / Registered nurses, 1976 / 30-55 / 0 / 469 / Registered nurses, 1976 / Age, smoking status, date of blood sampling, fasting status / ITA (Denka Seiken) / Denka Seiken / rs1130864, rs1205xiii
Speedwell Cohort / UK / Nested case-control / 238 / Non-fatal MI or CHD death (ICD9 410-414) / ECG, medical record, self-reported history and death certification / GP/hospital / 50-80 / 100 / 401 / Multiple / INA (Dade Behring) / Manufacturer / rs1130864, rs1205xi
Framingham Heart Study / USA / Cohort / 200 / Non-fatal MI or CHD death / Medical records, self-reported history and death certification / Framingham cohort: initial or 7th examination / 30-74 / 47 / 3139 / Framingham cohort: initial or 7th examination / Not matched / TF† / TF† / rs1130864, rs1205, rs3093077
Whitehall II / UK / Cohort / 160 / Non-fatal MI, ICD-9 410-414 / Symptoms, ECG, treatment history, death certification, hospital records verification / British civil servants / 35-85 / 75 / 3600 / British civil servants / Not matched / INA (Dade Behring) / Manufacturer / GD
NorthwickPark Heart-II Study / UK / Cohort / 103 / Non-fatal MI / ECG, enzymes, symptoms and death certification / Healthy middles-aged men / TF / 100 / 2573 / Healthy, middle-aged men / Not matched / TF (Cordia) / TF / rs1130864, rs1205, rs3093077xiv
Subtotal / 18,864 / 99,287
Retrospective studies
INTERHEART / 52 countries / Case-control / 4500 / non-fatal MI / Symptoms, cardiac enzymes, ECG / Coronary care unit or cardiology wards / 49-67 / 75 / 4500 / Hospital and community based / Age and sex / – / – / rs1130864, rs1205i
PROMIS / Pakistan / Case-control / 4000 / First onset acute MI / Symptoms, cardiac enzymes, ECG / 3 hospitals in Pakistan / 30-80 / 84 / 4000 / Hospital based / Age and sex / – / –
GISSI / Italy / Case-control / 3055 / non-fatal MI / Symptoms, cardiac enzymes, ECG / RCT-GISSI (Coronary care units) / 20-89 / 80 / 923 / Community based (blood donors) / Age and sex / TF / TF / GD
BHF/MRC Family Heart and GRACE Studies / UK / Case-control / 2000 / MI (premature CAD), history of CAD / Hospital records, GP verification / Media, mail to GPs / TF / 65 / 3000 / 1958BC, National Blood Service and sibling matches (FHS) / Not matched / – / – / GD
SHEEP / Sweden / Case-control / 1200 / First time non-fatal MI / Symptoms, cardiac enzymes, ECG / Hospitals in StockholmCounty / 45-70 / 70 / 1500 / StockholmCounty population register / Age, sex, residential area / – / – / rs1130864, rs1205iv
ISIS / UK / Case-control / 1000 / MI / ECG, Enzymes, Symptoms / Hospitals in UK / 30-64 / 55 / 1000 / Relation of cases / Not matched / TF / TF / GDv
Heart and Vascular Health Study / USA / Case-control / 850 / Non-fatal MI / Discharge abstracts, billing records, chart review / Regional health maintenance organization / 30-79 / 42 / 2688 / Regional health maintenance organization / Age, sex, hypertension, event year / – / – / rs1205, rs3093077vii
Leiceester/ Sheffield MI Cohort Study / UK / Case-control / 753 / Non-fatal MI / Symptoms, cardiac enzymes, ECG / Coronary care unit or cardiology wards / TF / 73 / 624 / Community based / Not matched / TF / TF / GD
CHAOS / England / Case-control / 500 / Non-total
MI / Symptoms, Cardiac enzymes, ECG / 3 hospitals in East Anglia / TF / TF / 1500 / MRC Ely cohort study: GP registers / Not matched / – / – / GD
Cupid/Cudas / Australia / Case-control / 485 / Non-fatal MI or CHD / Symptoms and hospital admissions / Hsopital recruitment / 26-77 / 100 / 502 / Community based / Not matched / ITA (Roche) / cFAS calibrator (CRM 470) / rs1130864
Subtotal / 18,343 / 20,237
TOTAL 35 studies / 37 207
coronary cases / 119524
controls

TF = to follow; For details of study acronyms, see list of Authors / Collaborators at end of manuscript

* Studies that have not yet extracted and arrayed DNA.

† Studies that have not yet provided data on whether they have recorded circulating CRP levels.

Other SNPs genotyped include: i INTERHEART: rs2808630, rs3093069, rs1800947, rs1417938, rs3091244, rs3093058; ii HIMS: rs1080947; iii Malmo: rs3091244; iv SHEEP: rs1800947, rs2794521; v ISIS: rs876537, rs1808628, rs2808630; vi BWHHS: rs1800947, rs2794521;

vii HVHS: rs3093058, rs3093059, rs3091244, rs1417938, rs1800947, rs3093066, rs2808631, rs2808630, rs3093075, rs3093077; viii PROSPER: rs1800947, rs1417938; ix NSC: rs1800947; x CHS: rs1800947, rs2608630, rs1417938; xi Caerphilly and Speedwell: rs1800947, rs2794521; xii Rotterdam: rs3093068; xiii HPFS and NHS: rs1800947, rs3093068, rs2808630, rs1417938; xiv NPHS II: rs3091244

TF: to follow; GD: generating data; ICD: International Classification of Diseases; CHD: coronary heart disease; MI: myocardial infarction; ECG: electrocardiograph; GP: general practitioner; MRC: Medical Research Council; INA: immunonephelometric assay; ITA: immunoturbidimetric assay; MEIA: Microparticle Enzyme Immunoassay.