Supplemental Table S1 Primers Utilized in This Work
Supplemental Table S1Primers utilized in this work
Primer / Sequence 5’ 3’cDNA cloning
upper / CAY TWC WSY GGH CAY GG
lower / CCR CTR TKR CAR GMR TC
ATTB1-Met / GGG GAC AAG TTT GTA CAA AAA AGC AGG CTT CAT GGC GAA GAA AGC AGT ATT AAT C
HA-MO-R / CTG GAA CAT CGT ATG GAT AGC ACA CAA AAG GAG CGT C
ATTB2-HA / GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC TCA AGC ATA ATC TGG AAC ATC G
5’-RACE
RACE5-N4 / CCA GTT TCA GCT GGA AGT CTA GT
RACE5-143 / CCG CTG TGG CAT GAG TCA GAT AC
3’-RACE
NT2B-F282 / GGT GAA GAT GAT GAT ACT GGT TAT GAT G
Mutagenesis
C139V-f / CAC AAT TGT ATC CGA CTC AGT GCA CAG CGG CGG CCT CAT TG
C139V-r / CAA TGA GGC CGC CGC TGT GCA CTG AGT CGG ATA CAA TTG TG
H86S-f / ATT CTT TGT TTG TTC ATT ACA GTG GAT CCG GGA CTA GAC TTC CAG CTG AAA C
H86S-r / GTT TCA GCT GGA AGT CTA GTC CCG GAT CCA CTG TAA TGA ACA AAC AAA GAA T
D348R-f / GTG GGT GCC AAA CGC GCC AAA CAT CTG CAG
D348R-r / CTG CAG ATG TTT GGC GCG TTT GGC ACC CAC
D353R-f / ACG GAC CAA ACA TCT GCA CGA GCT ACC CCT GCA GGC
D353R-r / GCC TGC AGG GGT AGC TCG TGC AGA TGT TTG GTC CGT
D348A-f / GTG GGT GCC AAA CGG CCC AAA CAT CTG CAG A
D348A-r / TCT GCA GAT GTT TGG GCC GTT TGG CAC CCA C
D353A-f / ACC AAA CAT CTG CAG CTG CTA CCC CTG CAG G
D353A-r / CCT GCA GGG GTA GCA GCT GCA GAT GTT TGG T
K224A-f / CCA TGT GAA GAA CGC ATC CTT GCC TCT TTC CAC CC
K224A-r / GGG TGG AAA GAG GCA AGG ATG CGT TCT TCA CAT GG
R187V-f / TGT TGA AGA CGC ATT TGA ATC GAT GGG TAT CCA CAT TCC ACG CC
R187V-r / GGC GTG GAA TGT GGA TAC CCA TCG ATT CAA ATG CGT CTT CAA CA
K270D-f / GTC CTA AGG TGA AGA AAT TCA TGG ACG TCA TTT TCA ACA AAC TAC AGC A
K270D-r / TGC TGT AGT TTG TTG AAA ATG ACG TCC ATG AAT TTC TTC ACC TTA GGA C