Positive Clonies and Confirmation

Tab. S1 PCR primers for the known genes in the SSH library specific to murine LDC

Clone
# /

Forward

Reverse

/ PCR
Size* / Annealing*
1 / CCG CAA CAT CAG CTC GTT ATC GGG ACA C / CTG TGA CTC CCA AGT GTG GAA GGG CAG AC / 384 / 60
4 / CAA GAG GTA GAA GGC TGT CTT CCA ATG G / ATC TGC TAG CGC ACT GCA GGT GAT GGA T / 210 / 55
7 / GAC TAC TGT TAG CCA GGC AGT GGT GGC GCA / GGG CTG AGA ATG AAG ACA GGA CAC AGC / 418 / 58
9 / GAG CTT TCC TAT TGA GCT GCC A / GCC TGT GAG TCC AAG GAA AGG A / 222 / 50
10 / GTG ACA GAT GAG GGC AGA AGA TAG GAC T / GTG TCA GAG TCT CTC TAT GTT CAC AGC CT / 226 / 58
11 / TGG AGG TGC CAG GTT TGA GTC C / GAA GAC CCT CAA GGC ATT GGT G / 167 / 50
14 / GAA CCA AGT GTT AGG AAT ATC CGC AGG CTG / GAC ACA TCT TCA TTG CGA GCG TCT CAT CGC A / 282 / 58
15 / GGT CTG ACA GCT CGG GTC CTC TGG / CGG ACC GCA AGC TGC GCT GTG AAG / 173 / 55
16 / CTT CTG AGC AGA TCA GAC TCT CCT CGT / CCC TCT CTG CTA GCC AGA CCA TCA GAT / 504 / 58
20 / CGA CAC TAA CAG GAC TGC ATA GAG / CAG CAA CAA CTT CAG CAG AGT TCC / 309 / 50
23 / GCT GTC CGA GGA GGC TCA GGA AGT TG / GGT ACC AAG GTG GCC AAG GGT TTG GA / 557 / 55
25 / GCT CTC AGT GAG GAC AGC CTA CGT CTC T / CCA TGG CAG AGT GCG CCT GCT GTT GAG / 510 / 55
32 / GAG CTA TGC AGC ATG GAA CAT GAC GAC / AGG AAA CTG CTA TCA TGA CTG GGG TGG / 342 / 58
38 / CCT GAC CAT GAT CAG AAA CCC GAG CTG A / GAC AGC CAT CTT GTC CTG TCC CTT CTT C / 337 / 58
48 / GTC CTT AGT TCA TGT GGC TCG GTC ACT TGG / GGT GTC GAT GCA AAC GTT CCC AAC TCA AGG / 303 / 58
51 / ACG GCC GTC AAC TTA ACC TAC / GCT GAC CAG AGC GTT TAG GAG / 206 / 50
53 / GTC CAC CAG TTC TAC CTG ATC C / CCT CAC CAG TCA AAC ATG GCC C / 419 / 55
56 / CAT GAG GCA GTT GAT CCT AGG C / GGT ATC CTG AAA GAA TGG CAG G / 217 / 50
62 / GCT GAG CGC TCC ATT CAT GAT GCT CTC TGT / GTC CTT CAT TCC AGC CAC AGT ACT TCT GTC / 484 / 55
66 / CAA GCT CTT GTG CAC AGA GTA GGC / GCT CAA GAG GCA GGT TCG ATG GAG / 196 / 58
80 / CTT GGT GAC AGA AAA GAC CTC AGG ACA CGC / AGC CAC TCC ACT GTC TCC TAC AGT AAC ACT C / 277 / 58
83 / CTG GTG GAA AGA GGG GAC TTG AAG C / ATG CTT CTG CGA CAT GGG ACT TGG C / 275 / 55
94 / GTG ACC CAA GCA CCA GGA AGT TAC / GGA GAA GAG ACC ACA GTA GCC TTG / 365 / 55
124 / CTG CCC TCG TTA CTT CCA GCT / CCC TTC TGT TCT CTC CTC AAC / 241 / 50
126 / CAT CAT AGT AGC TAG CTT TGA CGG C / TAT TCC GCA CTT GAA CAC GCC ACT TCC / 238 / 55
143 / CTG CTG GAC ATT GAG AAC AAG GGA TAC CTG A / GAT GCT TGA ATG CTG CAT GCA ACA CTG TTG / 466 / 55
151 / GGC GGG TGC AGT AAA ATG GGC GAT / GAC GAC TGT GAT ACT GTC CGT GCG G / 395 / 55

Tab. S2 PCR primers for the unknown genes in the SSH library specific to murine LDC

Clone / appearance /

Forward

/ Reverse / bp / annealing
12 / 1 / GGA TAA TAG GCA AGA CCA CTA GG / TGG AGT GTT GTG AGT TTA GCC T / 125 / 50
44 / 3 / CCT GAT CCT CCT GTC TGA CTG AGA / CGC CAG TGT GAT GGA TAT TTG CAG A / 319 / 58
52 / 1 / CGC GCT GGT TAA AGT CCG GGT / CGA TGG TGG GGG GAT TGA AGG / 177 / 55
58 / 1 / CTG GCT ACA CAC ACG ATC AGC TTT / CGC AGT CCG TGT AAG CAT GTT TAC / 234 / 53
69 / 1 / GCC ATG GCT GTC CTC CCC ACT TTG / GTC CCC GAG ACT TTC GCC TCA CCT / 343 / 58
79 / 1 / GAG CAA CTC CTG TCT CGG TGA TGT G / GCT CTA GTC CAT TTT TAG CTC CCG A / 332 / 55
110 / 1 / CCT TCA AGC ACA TAA GAA C / GAA TCG AGC AGT GTC TCA G / 254 / 60
145 / 1 / GCG TGG TCG CGG CCG AGG TAC CTT T / CAG AGG GTG ACA AGC AGA CAG GGC / 203 / 558
G3PDH / -- / CTC CCA CTC TTC CAC CTT CGA TGC / CCT CTC TTG CTC AGT GTC CTT GCT / 199 / 55

Tab. S3 Sequences occurred only one time in the LDC Library

Clone / Dot Blot / Size: Eco R I * / Size of Seq.
4 / +++ / 350 / 252
6 / ++ / 700 / 779
7 / + / 700 / 798
9 / ++ / 660 / 272
12 / ++ / 250 / 171
17 / + / 400 / 343
25 / - / 850 / 795
27 / - / 550 / 578
42 / + / 600 / 579
44 / + / 200 / 838
45 / + / 700 / 841
47 / +- / 250 / 307
49 / - / 450 / 391
52 / ++ / 350 / 308
53 / ++ / 900 / 845
58 / ++ / 800 / 853
59 / + / 780 / 844
62 / - / 200 / 167
68 / ++ / 200 / 228
69 / + / 580 / 551
70 / - / 900 / 706
74 / +- / 200 / 154
75 / ++ / 400 / 457
79 / ++ / 550 / 353
83 / - / 400 / 406
84 / + / 450 / 498
92 / ++ / 600 / 485
93 / + / 400 / 384
94 / +++ / 500 / 431
95 / +- / 600 / 435
97 / + / 500 / 378
100 / +- / 900 / 877
108 / +- / 600 / 506
110 / - / 500 / 364
115 / +- / 450 / 300
119 / - / 800 / 869
124 / +- / 300 / 306
126 / ++ / 350 / 274
127 / ++ / 400 / 273
128 / +- / 550 / 476
141 / - / 250 / 184
142 / +- / 450 / 439
143 / - / 480 / 466
145 / - / 300 / 237
150 / - / 200 / 230
151 / +- / 550 / 472
152 / +- / 300 / 234