Bio BasicInc.

NEXT GENERATION SEQUENCING ORDER FORM

(DNA SAMPLES)

  1. BASIC INFORMATION

NAME OF COMPANY
CONTACT PERSON
TElEPHONE NUMBER
EMAILADDRESS
aDDRESS
lab name
GROUP/p.i.
Research objective
  1. EXPERIMENTAL INFORMATION

BIORESEARCH FIELD / Genome
Transcriptome Level
Epigenetics
Other (Please specify below)
Other:
EXPERIMENTAL DESIGN / De Novo Sequencing / Genome Survey
Whole Genome Re-sequencing / Exome Sequencing
Whole Genome Bisulfite Sequencing / ChIP Sequencing
Metagonomic Sequencing / Genotyping
Target Region Sequencing / MeDIP Sequencing
Other:
  1. SAMPLE INFORMATION

TYPE OF SAMPLE / Genomic DNA
PCR Products
Broken Short Fragments
ChIP-Seq DNA Samples
Other (Please Specify Below)
Other:
STATE OF SAMPLE / Dissolved in Water
Dissolved in TE Buffer
Dissolved in Pure Ethanol
Other (Please Specify Below)
Other:
  1. SAMPLE DETAILS

Sample Name / Species / # of Tubes / Concentration (ng/µl) / Volume (µl) / Total Quantity (µg) / Fragment Size

Please send the completed form to:

SAMPLE CONDITION: DNA Samples without degradation or RNA contamination

SAMPLE QUANITITY: m≥6µg

SAMPLE CONCENTRATION: 50 ng/µl

SAMPLE PURITY: OD 260/280 = 1.8 ~ 2.0

  1. SAMPLE REQUIREMENTS

Library Types / Sample Types / Quantitative Methods / Quantitative Result
Qubit
≤800 bp Insert / Sample With Single Band or Main Apparent Band in Electrophoresis (genome, plasmid, PCR product) / Qubit / m≥6µg / c≥30µg
Sample for Meta-genomics Sequencing / Qubit / m≥4µg / c≥30µg
Samples With Smear in Electrophoresis: Not Fragmented / Qubit / m≥3µg / c≥30µg
Samples With Smear in Electrophoresis: Fragmented / Qubit / m≥4µg / c≥30µg
2K Insert / Genomic DNA / Qubit / m≥40µg / c≥133µg
5-6K Insert / Genomic DNA / Qubit / m≥40µg / c≥133µg
10K Insert / Genomic DNA / Qubit / m≥60µg / c≥133µg
20/40K Insert / Genomic DNA / Qubit / m≥120µg / c≥133µg
PCR Free / Genomic DNA / Qubit / m≥20µg / c≥30µg
PCR Product with Single Band / Qubit / m≥6µg / c≥30µg
ChIP-Seq / ChIP-Seq DNA Samples / Qubit, Agilent 2100 / m≥20µg
MeDIP/Bisulfite / Genomic DNA / Qubit / m≥10µg / c≥50µg
EXON Agilent, Nimblegen EZ, Nimblegen Array (pooling) / Genomic DNA / Qubit / m≥6µg / c≥50µg
EXON Nimblegen Array (one sample for Hybridization) / Genomic DNA / Qubi / m≥30µg / c≥50µg
Genotyping / Genomic DNA / Qubit, / m≥2µg / c≥50µg
  1. Terms and Conditions

Terms

Bio Basic Inc. will send electronically DNA sequencing results to customers. All results will be kept in Bio Basic Inc.’s data base for 6 months. During this period, customer may request sequencing results. After 6 months, DNA sequencing results are automatically destroyed without further notification.

DNA sequencing tests are not considered confirmed without the customer providing a P.O. number or credit card number. Bio Basic Inc. accepts credit card (Visa or MasterCard), cheque, or wire for payment.

Cancellations

Buyer may not cancel any order or return any samples without Bio Basic Inc.’s consent. Cancellation and return charges may be charged. Buyer must contact Bio Basic Inc. to obtain a return material authorization number.

Warranty

Bio Basic Inc. does not guarantee any downstream application(s) or usage of the DNA sequencing results. Any claims or dispute must be submitted within 2 weeks of the results being sent. At the time of shipment, Bio Basic Inc. keeps a database for unexpected incidents and/or disputes. Bio Basic Inc. reserves the right to refuse handling disputes after a period of 3 months.

Prices

All prices are subject to correction of errors; Bio Basic Inc. reserves the right to adjust prices on orders during production due to changes in cost of materials, transportation or wages. Any tax, customs, surcharge or duty, howsoever denominated, imposed upon the sale, importation, delivery or use of products shall be the responsibility of the Buyer.

20 Konrad Cres, MarkhamOntarioL3R 8T4Canada

Tel: (905) 474 4493, (800) 313 7224 Fax: (905) 474 5794

Email: Web: