Maldi-TOF Identification
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ORDER (please use the sample sheet)
The following options are available, please indicate your choice on the samplesheet:
- Identification of unknown bacteria/ yeast using the Maldi-TOF system:
- Pure culturing (optionally):
Depending on the wishes of the client all the contaminants, only the unique contaminants or the major contributorwill be re-cultured / -
RESULTS
After the results are released by QA the report will be send by email and by regular mail.I accept the general terms and conditions of
ProBasePharma*
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Date: _ _ / _ _ / _ _ _ _ / To be filled out by ProBase Pharma:
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*Our General Terms and Conditions apply to all projects ProBasePharma carries out and can be downloaded from our website (
SAMPLES
We can only process live Class I, non-pathogenic micro-organism that are provided to us according to Dutch regulations. In brief, this means that bacteria or fungi should be delivered on petri-dishes or on slants that are sealed with parafilm. Sealed dishes/ slants should be wrapped in absorbing tissue, and enclosed in an unbreakable and watertight cover. Make sure that you clearly indicate the sample names on the petri-dishes. Please note that the cultures should be fresh (<3 days old) for Maldi-TOF identification.
If you suspect the micro-organisms to be pathogenic please contact us beforehand so we can discuss alternative means of transportation.
TERMS OF DELIVERY
Bacterial or yeast identification using the Maldi-TOF (PB-310).
It is not possible to requests this service for mould samples, only bacterial and yeast samples will be identified using the Maldi-TOF system.
Maldi-TOF identification is achieved by obtaining spectra using the Maldi-TOF technology (Matrix Assisted Laser Desorption Ionization Time-of-Flight) and analyzing the spectra with the VITEK MS database. The peaks from these spectra are compared to the characteristic pattern for a species, genus, of family of micro-organism, thus resulting in an identification. If the Maldi-TOF results do not meet ProBase Pharma’sconfidence value, the samples will automatically be identified using 16s sequencing at no extra charge.
A full report with the results will be mailed to the client within 5 working days.
The reported result is the most likely sample identification. However, the client has to make the final conclusion to the identity of the sample.
Pure culture (PB-315)
When visually more than one type of clones are present on the sample plate the client will be contacted to discuss the different options.
-Option 1: Continue with the original plate and use the clones that are the most common on the plate for analysis
-Option 2: re-cultureall the different colonies to get several plates with (different)pure single colonies.
-Option 3: re-culture only the unique contaminants
-Option 4: re-culture only the most common colonies (major contributor)
Transport and Storage (To be filled in by client)
Transport:
liquid nitrogen Dry ice
Frozen (-25C and -15°C) Cooled (2-8C) Room temp. (15-25C)
Other, (please specify)
Temperature logger present yes no
Storage:
liquid nitrogenDeep frozen(<-60C)
Frozen (-25C and -15°C) Cooled (2-8C) Room temp. (15-25C)
Other,(please specify)
No
/Sample name
/Sample type
(Bacterial)
/Sample type(Fungal)
Only applicable for yeast samples, not for mould and filamentous fungi
/Please leave this column empty
1. / Bacterial / Fungal2. / Bacterial / Fungal
3. / Bacterial / Fungal
4. / Bacterial / Fungal
5. / Bacterial / Fungal
6. / Bacterial / Fungal
7. / Bacterial / Fungal
8. / Bacterial / Fungal
9. / Bacterial / Fungal
10. / Bacterial / Fungal
Remarks:
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