Higher Frequencies of HLA DQB1*05:01 and Anti-Glycosphingolipid Antibodies in a Cluster

Supplementary Material

Higher frequencies of HLA DQB1*05:01 and anti-glycosphingolipid antibodies in a cluster of severe Guillain-Barré syndrome

L. Schirmer, MD1, V. Worthington, PhD2, U. Solloch, PhD3, V. Loleit, MD1, V. Grummel1, N. Lakdawala, MSc2, D. Grant, MSc2, R. Wassmuth, MD4,5, A. H. Schmidt, MD, PhD3,4, F. Gebhardt, MD6, T. F. M. Andlauer PhD7,8, J. Sauter, PhD3, A. Berthele, MD1, M. P. Lunn, MD2, B. Hemmer, MD1,8

1 Department of Neurology, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany

2MRC Centre for Neuromuscular Diseases & Neuroimmunology and CSF Laboratory, National Hospital for Neurology, London, United Kingdom

3DKMS German Bone Marrow Center, Tübingen, Germany

4 DKMS Life Science Lab, Dresden, Germany

5 Department of Quality Management, University Hospital Düsseldorf, Düsseldorf, Germany

6 Institute for Medical Microbiology, Klinikum rechts der Isar, Technical University of Munich, Munich, Germany

7 Max Planck Institute of Psychiatry, Munich, Germany

8 Munich Cluster for Systems Neurology (SyNergy), Munich, Germany

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Supplementary table 1Detailed clinical, electrophysiological and CSF characteristics in GBS patients

Legend:f = female, m = male; n = no; y = yes; OCB = oligoclonal bands in CSF; identical = identical bands in both serum and CSF; CIDP = chronic inflammatory demyelinating polyneuropathy;n/a = not available.

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Supplementary table 2Anti-Cj and glycosphingolipidantibody reactivities and HLA class II typing in GBS patients

Legend:Cj: Campylobacter jejuni; n/a = not available; neg = negative; if applicable, borderline and anti-ganglioside/sulfatide IgG and IgM titers are shown in brackets; HLA alleles with identical nucleotide sequences for the peptide binding domain (exon 2) were grouped and designated by an upper case ‘G’ which follows the first 3 fields of the allele designation of the lowest numbered allele in the group; where a better typing resolution could be reached, generic codes for multiple allele designations (e.g., FNVX or HTC) were used.

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Supplementary table 3HLA II allele frequencies for the loci DRB1, DPB1 and DQB1 in cluster (n=13) and sporadic (n=16)GBS patients and control subjects (n=12602).

Legend: Note DRB1*01:01 and DQB1*05:01 allele frequencies are highlighted and shown in italic letters; alleles differing only by synonymous mutations or mutations outside exon 2 (peptide binding domain) were grouped and marked with ‘g’ as described before29.

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