W-171 Regional Research Project

Germ Cell and Embryo Development and Manipulation for the Improvement of Livestock

Publication Listing (January 1, 1999 through December 31, 2003)

ARKANSAS

a. Refereed Articles and Book Chapters:

Brown, A.H., D.W. Kellogg, Z.B. Johnson, R.W. Rorie, W.K. Coblentz, B.A. Sandelin, K.E. Lesmeister, and W.R. Jackson. 2001. Growth, luteal activity and pregnancy rates of three breed types of dairy heifers in a forage based development program. Prof. Anim. Sci. 17:20-26.

Burke, J.M. and R.W. Rorie. 2002. Changes in ovarian function in mature beef cows grazing endophyte-infected tall fescue. Theriogenology 57:1733-1742.

Burke, J.M., R.W. Rorie, E.L. Piper, and W.G. Jackson. 2001. Reproductive responses to grazing endophyte-infected tall fescue in postpartum beef cows. Theriogenology 56:357-369.

Kreider, D.L., R. Rorie, D. Brown, F. Miller, and S. Wright. 2001. Enhancement of ovulation rate and litter size in gilts. J. Anim. Sci. 79:1691-1696.

Looper, M.L., C.F. Rosenkrans, Jr., Z.B. Johnson, A.H. Brown, Jr., J.A. Hornsby, and J.L. Perkins. 2002. Relationships among growth characteristics of replacement Angus heifers. Prof. Anim. Sci. 18:120-126.

Melnychuk, V.L., M.A. Cooper, J.D. Kirby, R.W. Rorie, and N.B. Anthony. 2002. Use of ultrasonography to characterize of ovary status in chicken. J. Poultry Sci. 81:892-895.

Rorie, R.W., T.R. Bilby, and T.D. Lester. 2002. Application of electronic estrus detection technology to reproductive management of cattle. Theriogenology 57:137-148.

Rorie, R.W., T.D. Lester, B.R. Lindsey, and R.W. McNew. 1999. Effect of timing of artificial insemination on gender ratio in cattle. Theriogenology 52(6):1035-1041.

Rorie, R.W. Effect of timing of insemination on mammalian sex ratio. 1999. Theriogenology 52(8):1273-1280.

Steelman, C.D., R.W. McNew, R.B. Simpson, R.W. Rorie, J.M. Phillips and C.F. Rosenkrans, Jr. 2003. Evaluation of alternative tactics for management of insecticide-resistant horn flies (Diptera muscidae). J. Econ. Entomol. 96:892-901.

b. Books, Instructional Media, NonRefreed Book Chapters, Proceedings from Scientific Meetings, Theses and Dissertations:

Bilby, T.R. 2002. In vitro maturation of porcine oocyte-cumulus complexes. M.S. Thesis. University of Arkansas, Fayetteville.

Golden, C.D. Effects of ascorbic acid and alpha-tocopherol on physiological characteristics of boar spermatozoa. M.S. Thesis. University of Arkansas, Fayetteville.

Hazlett, W.D. 1999. Effect of rescue endophyte toxins on reproductive parameters in beef heifers. Ph.D. Dissertation. University of Arkansas, Fayetteville.

c. Abstracts:

Bilby, T.R. and R.W. Rorie. 2001. Effectiveness of maturational treatments to block porcine oocyte germinal vesicle breakdown without reducing subsequent nuclear maturation and cleavage. Theriogenology 55:463.

Bilby, T.R. and R.W. Rorie. 2001. Effects of sequence and timing of gonadotropin supple- mentation on porcine oocyte maturation and subsequent cleavage in vitro. J Anim. Sci. 79 (Suppl. 2):20.

Brown, A.H., D.W. Kellogg, Z.B. Johnson, R.W. Rorie, W.K. Coblentz, K.E. Lesmeister and W.R. Jackson. 2000. Growth, luteal activity and pregnancy rates of three breed types of dairy heifers. J. Anim. Sci. 78 (Suppl. 1):54.

Burke, J.M., R.W. Rorie, G.A. Perry, and D.H. Keisler. 2001. Changes in ovarian function in mature beef cows grazing endophyte-infected (E+) tall fescue. Biol. Reprod. 64 (Suppl.1):344.

Burke, J.M., R.W. Rorie, E.L. Piper, and W.G. Jackson. 2000. Effects of fescue toxicosis on reproductive responses in the postpartum beef cow. J. Anim. Sci. 78 (Suppl. 2):7.

Lester, T.D., R.W. McNew, and R.W. Rorie. 1999. Use of electronic estrous detection to evaluate the effect of embryo-recipient synchrony on pregnancy rate in cattle. Theriogenology 51(1):265.

Lorton, L.L., T. Lester, C.F. Rosenkrans, Jr., and R.W. Rorie. 1999. Effect of estrous parameters and time of insemination on pregnancy rate in beef cattle. J. Anim. Sci. 77 (Suppl. 1):15 (abstract 59).

Rorie, R.W., W. Hazlett, T.D. Lester, and D. Kreider. 1999. Effect of endophyte-infected fescue on ovarian function in beef heifers. J. Anim. Sci. 77 (Suppl. 1):15 (abstract 58).

Rorie, R.W., T.D. Lester, B.R. Lindsey, and R.W. McNew. 1999. Effect of timing of artificial insemination on gender ratio in cattle. Theriogenology 51(1):299.

Wright, S., D. Kreider, and R. Rorie. 1999. Evaluation of a two-part melengesterol acetate regimen to synchronize estrus in beef cows. J. Anim. Sci. 77 (Suppl. 1):221.

d. Miscellaneous Publications (Semi-Technical/Lay Publications):

Bilby, T.R. and R.W. Rorie. 2001. Effect of treatment to temporary block germinal vesicle breakdown on porcine oocyte maturation and subsequent parthenogenetic development. Arkansas Animal Science, Research Series 488:45-48.

Brown, A.H. Jr., D.W. Kellogg, Z.B. Johnson, R.W. Rorie, W.K. Coblentz, B.A. Sandelin, and K.E. Lesmeister. 2001. Growth, luteal activity, and pregnancy rates of three breeds of dairy heifers in a forage-based development program. Arkansas Animal Science, Research Series 488:155-159.

Golden, C, C Rosenkrans, Jr., and Z Johnson. 2002. Effects of ascorbic acid and alpha-tocopherol on cryopreserved boar sperm. Arkansas Animal Science, Research Series 499: 120-123.

Kreider, D.L., R. Rorie, N. Post, and K. Cole. 2001. Supplementation of beef cows and heifers with high quality fescue hay. Arkansas Animal Science, Research Series 488:56-60.

Kreider, D., R. Rorie, D. Brown, F. Miller, and S.Wright. 2000. Enhancement of ovulation rate and litter size in swine. Arkansas Animal Science, Research Series 478:138-142.

Miller, G.F. and R.W. Rorie. 2000. Effect of oxygen concentration during oocyte maturation on subsequent bovine embryo cleavage and development in vitro. Arkansas Animal Science, Research Series 478:43-44.

Post, N.M., D.L. Kreider, R. Rorie, T. Lester, and K. Cole. 2002. Estrus synchronization and timed artificial insemination with melengesterol acetate plus GnRH in beef females. Arkansas Animal Science, Research Series 499:126-129.

R.W. Rorie, T.R. Bilby and T.D. Lester. 2002. Comparison of electronic mount monitors for detection of estrus in cattle. Arkansas Animal Science, Research Series 499:124-125.

Rorie, R.W. and T.D. Lester. 1999. Effect of estrous parameters and time of insemination on pregnancy rate in beef cattle. Arkansas Animal Science, Research Series 470:50-52.

Rorie, R.W. and T.D. Lester. 2000. The use of an electronic estrus detection system to evaluate the Effect of embryo-recipient synchrony on pregnancy rate in cattle. Arkansas Animal Science, Research Series 478:45-47.

R.W. Rorie and T.D. Lester. 2002. Comparison of lutalyse and estrumate for inducing estrus in beef cows and heifers. Arkansas Animal Science, Research Series 499:130-131.

Rorie, R.W. and T.D. Lester. 1999. Effect of timing of artificial insemination on gender ratio in beef cattle. Arkansas Animal Science, Research Series 470:47-49.

Wright, S, D. Kreider, R. Rorie, N. Huber, and G. Murphy. 1999. Evaluation of a two-part melengesterol acetate synchronization regimen. Arkansas Animal Science, Research Series 470: 53-55.

CALIFORNIA

a. Refereed Articles and Book Chapters:

Anderson, G.B. 1999. Embryonic stem cells in agricultural species. Transgenic Animals in Agriculture. CAB International Press., pp. 57-66.

Berger, T. 1998. Male effects on reproductive performance. J. Anim. Sci. 76 (Suppl. 3): 47-51.

Berger, T., and M. Dally. 2001. Do siredam interactions contribute significantly to fertility comparisons in heterospermic insemination trials? Theriogenology 56:535543.

Bertolini, M., J.B. Mason, S.E. Beam, G.F. Carneiro, M.L. Sween, D.J. Kominek, A.L. Moyer, T.R. Famula, R.D. Sainz, and G.B. Anderson. 2002. Morphology and morphometry of in vitro-and in vitro-produced concepti from early pregnancy to term and association with high birth weights. Theriogenology 58:973-994.

Bertolini, M., S.E. Beam, H. Shim, L.R. Bertolini, A.L. Moyer, T.R. Famula, and G. B. Anderson. 2002. Growth, development and gene expression by in vitro-and in vitro-produced day-7 and day-16 bovine embryos. Molec. Reprod. Develop. 63:318-328.

Cargill, S.L., J.R. Carey, H.G. Muller, and G. Anderson. 2003 Age of ovary determines remaining life expectancy in old ovariectomized mice. Aging Cell 2:185-190.

Carmona, E., W. Weerachatyanukul, T. Soboloff, A.L. Fluharty, D. White, L. Promdee, M. Ekker, T. Berger, M. Buhr, and N. Tanphaichitr. 2002. Arylsulfatase A is present on the pig sperm surface and is involved in sperm-zona pellucida binding. Develop. Biol. 247:182-196.

Carneiro, G., P. Lorenzo, C. Pimental, L. Pagoraro, M. Bertolini, B. Ball, G. Anderson, and I. Liu. 2001. The influence of insulin-like growth factor-I and its interaction with gonadotropins, estradiol and fetal calf serum and in vitro maturation and parthenogenic development in equine oocytes. Biol. Reprod. 65:899-905.

Chen, L.R., Y.L. Shiue, L. Bertolini, J.F. Medrano, R.H. BonDurant, and G.B. Anderson. 1999. Establishment of pluripotent cell lines from porcine preimplantation embryos. Theriogenology 52:195-212.

Choi, S.-J., and G.B. Anderson. 1999. Lack of stage-specific embryonic antigen expression by bovine embryos and primordial germ cells. J. Dairy Sci. 82:516-519.

Fieni, F., J. Rowe, K. VanHoosear, C. Burucoa, S. Oppenheim, G. Anderson, J. Murray, and R. BonDurant. 2002. Presence of caprine arthritis-encephalitis virus (CAE) infected cells in flushing media following oviductal-stage embryo collection. Theriogenology 57:931-940.

Gravance, C.G., D.L. Garner, M.G. Miller, and T. Berger. 2001. Fluorescent probes and flow cytometry to assess rat sperm integrity and mitochondrial function. Reprod. Tox. 15:510.

Gutierrez-Adan, A., E.A. Maga, E. Behboodi, J.S. Conrad, A.G. MacKinlay, G.B. Anderson, and J.D. Murray. 1999. Expression of bovine alpha-lactoglobulin in the milk of transgenic mice. Dairy Res. 66:289-294.

Jelks, K., T. Berger, C. Horner, and M.G. Miller. 2001. Chlorohydrin induced changes in sperm fertilizing ability in the rat: association with diminished sperm ATP levels and motility. Reprod. Tox. 15:1120.

Linfor, J., and T. Berger. 2000. Potential role of alpha v and beta 1 integrins as oocyte adhesion molecules during fertilization in pigs. J. Reprod. Fertil. 120:65-72.

Maga, E.A., G. Sargent, H. Zeng, S. Pati, D. Zarling, S.M. Oppenheim, N.M. B. Collette, A.L. Moyer, J.S. Conrad-Brink, J.D. Rowe, R.H. BonDurant, G.B. Anderson, and J. D. Murray. 2003. Increased efficiency of transgenic livestock production. Transgenic Res. 12:485-496.

Rui, R., H. Shim, A.L. Moyer, D. Anderson, M.C. Penedo, J.D. Rowe, R.H. BonDurant, and G.B. Anderson. 2003. Attempts to enhance production of chimeras from porcine embryonic germ cells and preimplantation porcine embryos. Theriogenology (In press)

Sartini, B.L., and T. Berger. 2000. Identification of homologous binding proteins in porcine and bovine gametes. Molec. Reprod. Develop. 55:446-451.

Sartini, B.L. and T. Berger. 2003. Changes in oocyte plasma membrane binding sites on boar spermatozoa with capacitation and acrosome reactions. Reproduction 125:865-870.

Stoops, M.A., G.B. Anderson, B.L. Lasley, and S.E. Shideler. 1999. Estimating the pregnancy rate of a free-ranging herd of Tule elk using fecal steroid metabolites. J. Wildlife Mgmt. 63:561-569.

Walters, K.W., C.J. Corbin, G.B. Anderson, J.F. Roser, and A.J. Conley. 2000. Tissue-specific localization of cytochrome P450 aromatase in the equine embryo by in situ hybridization and immunocytochemistry. Biol. Reprod. 62:1141-1145.

Ward, E., and T. Berger. 2000. Binding of porcine sperm plasma membrane proteins to sheep, hamster and mouse oocyte plasma membrane. Zygote 8:181-187.

Sartini, B. L., B. Schäfer, C. Kirchhoff, and T. Berger. 2003. Porcine sperm plasma membrane proteins containing Fn2 modules are potentially involved in gamete membrane interaction during fertilization. Biol. Reprod. (Submitted)

Sartini, B.L., and T. Berger. 2003. SE12, Candidate oocyte fusion proteins on the sperm plasma membrane are added to boar sperm in the epididymis. Molec. Reprod. Develop. (Submitted)

b. Books, Instructional Media, NonRefreed Book Chapters, Proceedings from Scientific Meetings, Theses and Dissertations:

Anderson, G.B. 2000. What lies ahead for embryo manipulation and transfer. Proceedings 19th American Embryo Transfer Association. pp.1-8.

Bender, Tad. 2002. Co-localization of two procine oocyte plasma membrane receptors for sperm during maturation. M.S. Thesis. University of California, Davis.

Berger, T., and M. Dally. 2001. Is there a sire-dam interaction in sperm fertilizing potential? California Agriculture 55:25.

Bertolini, Marcelo. 2002. Physiological and molecular mechanisms underlying the appearance of the Large Calf Syndrome for in vitro-produced bovine embryos. Ph.D. Dissertation. University of California, Davis.

Bertolini, M., T.R. Famula, and G.B. Anderson. 2000. Appearance of giant cotyledons in the large calf syndrome. Proc.Western Sect. Amer. Soc. Anim. Sci. 51:65-68.

Lopes, Rui. 2002. Identification of complementary gamete adhesion molecules using a protein expression cDNA library. M.S. Thesis. University of California, Davis.

Murray, J.D., and G.B. Anderson. 2000. Genetic engineering and cloning may improve milk, livestock production. California Agriculture 54:57-65.

Oppenheim, S.M. 1999. The hematopoietic chimera: A model to study sheep-goat interspecies and hybrid pregnancy failure. PhD Dissertation. University of California, Davis.

Sartini, Becky. 2002. Characterization of boar sperm plasma membrane candidate oocyte membrane fusion proteins and investigation of oocyte membrane binding sites in boar sperm populations. Ph.D. Dissertation. University of California, Davis.

Yen, Chun-Che. 1999. Localization of a 56 kD porcine oocyte membrane protein (POMP) involved in sperm-oocyte binding. M.S. Thesis. University of California, Davis.

c.Abstracts:

At-Taras, E.E., T. Berger, and J.F. Roser. 2002. Effects of an aromatase inhibitor on testicular steroids and gonadotropins in adult and neonatal boars. Biol. Reprod. 66 (Suppl 1): 282.

Berger, T., C.M. Horner, K. A. Jelks, B.S. Winder, and M.G. Miller. 1999. Perchloroethylene reduces rat sperm fertilizing potential. Tox. Sci. 48 (1S): 382.

Berger, T., M. B. Horton, and B. J. Nitta. 1999. Inhibition of sperm-oocyte interaction by an antibody to a porcine oocyte plasma membrane protein (POMP). Biol. Reprod. 60 (Suppl. 1): 105.

Berger, T., B.L. Sartini, and W.G. Breed. 2002. The porcine sperm DRKD proteins: presence of antigenically related proteins in marsupial sperm plasma membrane. Biol. Reprod. 66 (Suppl 1): 256.

Berger, T., C. Leigh, and W.G. Breed 2002. Sperm ligands for the marsupial zona pellucida

(ZP). 9th International Symposium on Spermatology p. 72.

Carmona, E., W. Weerachatyanukul, T. Soboloff, A. Fluharty, D. White, L. Promdee, M. Ekker, T. Berger, M. Buhr, and N. Tanphaichitr. 2002. Arylsulfatase A is present on the pig sperm surface and is involved in sperm-zona pelludica binding via ZP3 interaction. Biol. Reprod. 66 (Suppl 1): 273.

Ducummon, C. and T. Berger (2001) Cytotoxic necrotizing factor, a bacterial activator of the monomeric Gprotein RhoA, enhances spontaneous acrosome reactions in porcine sperm. Biol. Reprod. 64 (Suppl 1):300.

Ducummon, C., M.B. Horton, and T. Berger. 2000. The monomeric G-protein RhoA undergoes translocation during capacitation of porcine sperm. Biol. Reprod. 62 (Suppl 1):208.

Gravance, C.G.,M.G. Miller, T. Berger, and D.L. Garner. 1999. Detection of toxicant induced changes of rat sperm mitochondrial membrane potential by flow cytometry. Tox. Sci. 48 (1S): 382.

Khalil, M.B., K. Chakrabandhu, E. Carmona, H Xu, N. Vuong, P. Kumarathasan, S. da Silva, D.

Carrier, P. Wong, M. Buhr, T. Berger, and N. Tanphaichitr. 2003. Pig sperm rafts exhibit zona-

pellucida binding ability and contain mammalian male germ cell specific sulfogalactosyl-

glycerolipid and arylsulfatase A. Biol. Reprod. 68 (Suppl 1): 167.

McCarthy, M., E.E. At-Taras, B.J. Nitta-Oda, C. Ducummon, C. Pearl, J.F. Roser, and T. Berger.

2003. Reduction of endogenous estrogens reduces epididymal boar sperm fertility. Biol. Reprod.

68 (Suppl 1): 301.

Sartini, B.L. and T. Berger (2001) Localization of DRKD sperm protein on boar sperm. Biol. Reprod. 64 (Suppl 1): 277.

Williams, J., B.L. Sartini, and T. Berger. (2001) flow cytometric analysis of boar sperm mitochondrial function with JC1. Biol. Reprod. 64 (Suppl 1): 301302.

Yen, C.C., and T. Berger. 1999. Localization of a 56-kDa porcine oocyte membrane protein (POMP) involved in sperm-oocyte binding. Biol. Reprod. 60 (Suppl. 1): 106-107.

COLORADO

a. Refereed Articles and Book Chapters:

Amann, R. P., G. E. Seidel, Jr., and Z. Brink. 1999. Exposure of thawed frozen bull sperm to a synthetic peptide before artificial insemination increases fertility. J. Androl. 20:42-46.

Amann, R. P., G. E. Seidel, Jr., and R. G. Mortimer. 2000. Fertilizing potential in vitro of semen from young beef bulls containing a high or low percentage of sperm with a proximal droplet. Theriogenology 54:1499-1515.

Bass, L. D., D. J. Denniston, L. J. Maclellan, P. M. McCue, G. E. Seidel, Jr., and E. L. Squires. 2003. Methanol as a cryoprotectant for equine embryos. Theriogenology (In press).

Buchanan, B. R., G. E. Seidel, Jr., P. M. McCue, J. L. Schenk, L. A. Herickhoff, and E. L. Squires. 2000. Insemination of mares with low numbers of either unsexed or sexed spermatozoa. Theriogenology 53:1333-1344.

Choi, Y. H., Y. G. Chung, G. E. Seidel, Jr., and E.L. Squires. 2001. Developmental capacity of equine oocytes matured and cultured in equine trophoblast-conditioned media. Theriogenology 56:329-339.

Choi, Y. H., E. M. Carnevale, G. E. Seidel, Jr., and E. L. Squires. 2001. Effects of gonadotropins on bovine oocytes matured in TCM-199. Theriogenology 56:661-670.

Choi, Y. H., F. C. Landim-Alvarenga, G. E. Seidel, Jr., and E. L. Squires. 2003. Effect of capacitation of stallion sperm with polyvinylalcohol or bovine serum albumin on penetration of bovine zona-free or partially zona-removed equine oocytes. J. Anim. Sci. 81:2080-2087.

Cibelli, J.B., K.H. Campbell, G.E. Seidel, M. D. West, and R. P. Lanza. 2002. The health profile of cloned animals. Nature Biotechnol. 20:13-14.

Coutinho da Silva, M. A., E. M. Carnevale, L. J. Maclellan, G. E. Seidel, Jr., and E. L. Squires. 2002. Effect of time of oocyte collection and site of insemination on oocyte transfer in mares. J. Anim. Sci. 80:1275-1279.

Coutinho da Silva, M. A., E. M. Carnevale, L. J. Maclellan, K. A. Preis, G. E. Seidel, Jr., and E. L. Squires. 2003. Oocyte transfer in mares with intrauterine or intraoviductal insemination using fresh, cooled and frozen stallion semen. Theriogenology (In press).

Flint, A. F., P. L. Chapman, and G. E. Seidel, Jr. 2003. Heterospermic insemination of cattle using flow-sorted sperm. J. Anim. Sci. 81:1814-1822.

Franz, L. C., Y.-H. Choi, E. L. Squires, G. E. Seidel, Jr., and K. Hinrichs. 2003. Effects of roscovitine on maintenance of the germinal vesicle in horse oocytes, subsequent nuclear maturation, and cleavage rates after intracytoplasmic sperm injection. Reproduction 125:693-700.

Garner, D. L., and G. E. Seidel, Jr. 2003. Past, present and future perspectives on sexing sperm. Can. J. Anim. Sci. 83:375-384.

Glover, M. D. and G. E. Seidel, Jr. 2003. Increased messenger RNA for allograft inflammatory factor-1, LERK-5, and a novel gene in 17.5-day relative to 15.5-day bovine embryos. Biol. Reprod. 69:1002-1012.

Hurtt, A. E., F. Landim-Alvarenga, G. E. Seidel, Jr., and E. L. Squires. 2000. Vitrification of immature and mature equine and bovine oocytes in an ethylene glycol, ficoll and sucrose solution using open-pulled straws. Theriogenology 54:119-128.

Johnson, L. A., and G. E. Seidel, Jr. 1999. Current status of sexing mammalian sperm. Theriogenology 52:1267-1457.

Landim-Alvarenga, F. C., M. A. Alvarenga, G. E. Seidel, Jr., E. L. Squires, and J. K. Graham. 2001. Penetration of zona-free hamster, bovine and equine oocytes by stallion and bull spermatozoa pretreated with equine follicular fluid, dilauroylphosphatidylcholine or calcium ionophore A23187. Theriogenology 56:937-953.

Landim-Alvarenga, F. C., S. E. A. Boyazoglu, L. R. Carvalho, Y. H. Choi, E. L. Squires, and G. E. Seidel, Jr. 2002. Effects of fetuin on zona pellucida hardening, fertilization and embryo development in cattle. Anim. Reprod. Sci. 71:181-191.

Lane, M., M. K. O’Donovan, E. L. Squires, G. E. Seidel, Jr., and D. K. Gardner. 2001. Assessment of metabolism of equine morula and blastocysts. Molec. Reprod. Develop. 59:33-37.

Li, G.-P., G. E. Seidel, Jr., and E. L. Squires. 2003. Intracytoplasmic sperm injection of bovine oocytes with stallion spermatozoa. Theriogenology 59:1143-1155.

Li, G.-P., G. E. Seidel, Jr., and E. L. Squires. 2003. Improved cleavage of bovine ICSI ova cultured in heparin-containing medium. Theriogenology (In press).

Lindsey, A.C., D.D. Varner, G. E. Seidel, Jr., J. E. Bruemmer, and E. L. Squires. 2003. Hysteroscopic or rectally guided, deep-uterine insemination of mares with spermatozoa stored at 18 h at either 5ºC or 15ºC prior to flow-cytometric sorting. Anim. Reprod. Sci. (Submitted).

Lu, K. H., D. G. Cran, and G. E. Seidel, Jr. 1999. In vitro fertilization with flow-cytometrically-sorted bovine sperm. Theriogenology 52:1393-1406.

Mendes, J. O. B., Jr., P. D. Burns, J. F. De La Torre-Sanchez, and G. E. Seidel, Jr. 2003. Effect of heparin cleavage rates and embryo production with four bovine sperm preparation protocols. Theriogenology 60:331-340.

Oberstein, N., M. K. O’Donovan, J. E. Bruemmer, G. E. Seidel, Jr., E. M. Carnevale, and E. L. Squires. 2001. Cryopreservation of equine embryos by open pulled straw, cryoloop, or conventional slow cooling methods. Theriogenology 55:607-613.

Olson, S.E., and G. E. Seidel, Jr. 2000. Culture of in vitro-produced bovine embryos with vitamin E improves development in vitro and after transfer to recipients. Biol. Reprod. 62:248-252.

Olson, S. E., and G. E. Seidel, Jr. 2000. Reduced oxygen tension and EDTA improve bovine zygote development in a chemically defined medium. J. Anim. Sci. 78:152-157.

Schenk, J. L., T. K. Suh, D. G. Cran, and G. E. Seidel, Jr. 1999. Cryopreservation of flow-sorted bovine spermatozoa. Theriogenology 52:1375-1392.

Seidel, G. E., Jr. 1999. Sexing mammalian spermatozoa and embryos -- State of the art. J. Reprod. Fertil. (Suppl. 54):475-485.

Seidel, G. E., Jr. 2000. Reproductive biotechnology and the “big” biological questions. Theriogenology 53:187-194.

Seidel, G. E., Jr. 2001. Cloning, transgenesis, and genetic variance in animals. Cloning & Stem Cells 4:251-256.

Seidel, G. E., Jr., 2003. Economics of selecting for sex: The most important genetic trait. Theriogenology 59:585-598.

Seidel, G. E., Jr. 2003. Sexing mammalian sperm — Intertwining of commerce, technology, and biology. Anim. Reprod. Sci. (In press)

Seidel, G. E., Jr., and L. A. Johnson. 1999. Sexing mammalian sperm – Overview. Theriogenology 52:1267-1272.

Seidel, G. E., Jr., J. L. Schenk, L. A. Herickhoff, S. P. Doyle, Z. Brink, R. D. Green, and D. G. Cran. 1999. Insemination of heifers with sexed sperm. Theriogenology 52:1407-1420.

Seidel, G. E., Jr., and D. L. Garner. 2002. Current status of sexing mammalian spermatozoa. Reproduction 124:733-743.

Zhang, M., K. H. Lu, and G. E. Seidel, Jr. 2003. Development of bovine embryos after in vitro fertilization of oocytes with flow cytometrically-sorted, stained, and unsorted sperm from different bulls. Theriogenology 60:1657-1663.