Table S7Total number of base pairs found at the interface regions between double-stranded and single-stranded regions (bulges and internal loops only). Only one base pair at the vicinity of these regions was considered. The observed and expected values of GC, GU and AU pairs at these regions are shown, including the results of a chi-square test comparing them. GU and AU pairs were grouped together since both are less stable than GC pairs and because UG ↔ UA and GU ↔ AU are the most common hemi-CBCs found in this dataset (Table S9). Bold numbers indicate significant results at p<0.05 (**) and p<0.10 (*)

Species / Total pairs at interface regions / Total pairs (observed) / Total pairs (expected) / chi-square
GC / GU / AU / GU+AU / GC / GU / AU / GU+AU / GC / GU+AU / Total / p-value(1d.f.)
Calliphora vomitoria / 18 / 6 / 0 / 12 / 12 / 3 / 1.16667 / 13.8333 / 15 / 3 / 0.6 / 3.6 / p<0.06 *
Aldrichina grahami / 28 / 9 / 3 / 16 / 19 / 5.18519 / 1.81481 / 21 / 22.8148 / 2.80661 / 0.63787 / 3.44448 / p<0.07 *
Calliphora vicina / 20 / 5 / 2 / 13 / 15 / 2.77778 / 1.85185 / 15.3704 / 17.2222 / 1.77778 / 0.28674 / 2.06452 / p<0.20
Lucilia caesar / 26 / 7 / 1 / 18 / 19 / 4.09259 / 1.68519 / 20.2222 / 21.9074 / 2.06544 / 0.38585 / 2.4513 / p<0.15
Lucilia illustris / 23 / 6 / 1 / 16 / 17 / 3.57282 / 1.78641 / 17.6408 / 19.4272 / 1.6489 / 0.30325 / 1.95215 / p<0.20
Hemipyrellia ligurriens / 24 / 5 / 2 / 17 / 19 / 3.63636 / 1.21212 / 19.1515 / 20.3636 / 0.51136 / 0.09131 / 0.60268 / p<0.50
Lucilia cuprina / 22 / 6 / 0 / 16 / 16 / 4.04082 / 0.67347 / 17.2857 / 17.9592 / 0.94991 / 0.21373 / 1.16364 / p<0.30
Lucilia sericata / 22 / 6 / 1 / 15 / 16 / 3.96 / 1.32 / 16.72 / 18.04 / 1.05091 / 0.23069 / 1.2816 / p<0.30
Lucilia ampullacea / 20 / 7 / 1 / 12 / 13 / 3.55556 / 0.88889 / 15.5556 / 16.4444 / 3.33681 / 0.72147 / 4.05828 / p<0.05 **
Lucilia porphyrina / 20 / 7 / 0 / 13 / 13 / 3.61702 / 0.6383 / 15.7447 / 16.383 / 3.16408 / 0.69856 / 3.86264 / p<0.05 **
Lucilia bazini / 16 / 6 / 0 / 10 / 10 / 2.90909 / 1.09091 / 12 / 13.0909 / 3.28409 / 0.7298 / 4.01389 / p<0.05 **
Lucilia eximia / 26 / 6 / 0 / 20 / 20 / 4.05505 / 1.19266 / 20.7523 / 21.945 / 0.93287 / 0.17238 / 1.10525 / p<0.30
Chrysomya megacephala / 18 / 6 / 1 / 11 / 12 / 2.94231 / 1.90385 / 13.1538 / 15.0577 / 3.1776 / 0.62091 / 3.79851 / p<0.06 *
Chrysomya bezziana / 18 / 6 / 1 / 11 / 12 / 2.97087 / 1.74757 / 13.2816 / 15.0291 / 3.08852 / 0.61052 / 3.69904 / p<0.06 *
Chrysomya saffranea / 18 / 6 / 1 / 11 / 12 / 2.88679 / 1.86792 / 13.2453 / 15.1132 / 3.35738 / 0.6413 / 3.99868 / p<0.05 **
Chrysomya pinguis / 16 / 6 / 1 / 9 / 10 / 2.64078 / 1.5534 / 11.8058 / 13.3592 / 4.27313 / 0.84469 / 5.11782 / p<0.03 **
Chrysomya latifrons / 14 / 6 / 0 / 8 / 8 / 2.37736 / 1.84906 / 9.77358 / 11.6226 / 5.52022 / 1.12914 / 6.64935 / p<0.01 **
Chrysomya semimetallica / 16 / 6 / 1 / 9 / 10 / 2.51852 / 2.22222 / 11.2593 / 13.4815 / 4.81264 / 0.89906 / 5.7117 / p<0.02 **
Chrysomya albiceps / 22 / 7 / 0 / 15 / 15 / 3.56757 / 0.99099 / 17.4414 / 18.4324 / 3.30242 / 0.63918 / 3.94159 / p<0.05 **
Chrysomya rufifacies / 23 / 7 / 0 / 16 / 16 / 3.83333 / 1.49074 / 17.6759 / 19.1667 / 2.61594 / 0.52319 / 3.13913 / p<0.08 *
Chrysomya incisuralis / 26 / 8 / 0 / 18 / 18 / 4.14159 / 1.38053 / 20.4779 / 21.8584 / 3.59458 / 0.68108 / 4.27566 / p<0.04 **
Chrysomya nigripes / 24 / 5 / 0 / 19 / 19 / 3.70909 / 2.83636 / 17.4545 / 20.2909 / 0.44929 / 0.08213 / 0.53141 / p<0.50
Chrysomya putoria / 24 / 8 / 1 / 15 / 16 / 4.23529 / 1.64706 / 18.1176 / 19.7647 / 3.34641 / 0.71709 / 4.06349 / p<0.05 **
Chrysomya flavifrons / 20 / 6 / 0 / 14 / 14 / 3.11927 / 1.46789 / 15.4128 / 16.8807 / 2.66044 / 0.4916 / 3.15205 / p<0.08 *
Chrysomya varipes / 18 / 7 / 0 / 11 / 11 / 2.94231 / 1.55769 / 13.5 / 15.0577 / 5.5959 / 1.09345 / 6.68935 / p<0.01 **
Cochliomyia hominivorax / 20 / 4 / 1 / 15 / 16 / 2.90323 / 1.45161 / 15.6452 / 17.0968 / 0.41434 / 0.07036 / 0.4847 / p<0.50
Cochliomyia macellaria / 20 / 4 / 0 / 16 / 16 / 2.72 / 1.6 / 15.68 / 17.28 / 0.60235 / 0.09481 / 0.69717 / p<0.50
Hemilucilia segmentaria / 22 / 6 / 1 / 15 / 16 / 3.33929 / 1.57143 / 17.0893 / 18.6607 / 2.12003 / 0.37937 / 2.49941 / p<0.15
Chloroprocta idioidea / 24 / 6 / 1 / 17 / 18 / 3.74312 / 1.10092 / 19.156 / 20.2569 / 1.36077 / 0.25145 / 1.61221 / p<0.30
Phormia regina / 20 / 6 / 0 / 14 / 14 / 2.83186 / 1.76991 / 15.3982 / 17.1681 / 3.54436 / 0.58464 / 4.12899 / p<0.05 **
Protophormia terraenovae / 22 / 6 / 1 / 15 / 16 / 3.25926 / 1.22222 / 17.5185 / 18.7407 / 2.30471 / 0.40082 / 2.70553 / p<0.15
Mesembrinella peregrina / 28 / 8 / 0 / 20 / 20 / 5.01053 / 1.17895 / 21.8105 / 22.9895 / 1.78364 / 0.38874 / 2.17238 / p<0.15
Mesembrinella bellardiana I / 26 / 6 / 3 / 17 / 20 / 5.77778 / 1.68519 / 18.537 / 20.2222 / 0.00855 / 0.00244 / 0.01099 / p<0.99
Mesembrinella bellardiana II / 28 / 6 / 2 / 20 / 22 / 5.65657 / 1.69697 / 20.6465 / 22.3434 / 0.02085 / 0.00528 / 0.02613 / p<0.90
Stomoxys calcitrans / 18 / 6 / 1 / 11 / 12 / 3.375 / 1.875 / 12.75 / 14.625 / 2.04167 / 0.47115 / 2.51282 / p<0.15
Musca domestica / 22 / 7 / 2 / 13 / 15 / 4.97391 / 1.72174 / 15.3043 / 17.0261 / 0.82531 / 0.2411 / 1.06641 / p<0.40
Haematobia irritans / 22 / 5 / 2 / 15 / 17 / 4.31776 / 2.46729 / 15.215 / 17.6822 / 0.1078 / 0.02632 / 0.13412 / p<0.80