Supplemental Table S1. Bacterial strains and plasmids used in this study.

Strain or plasmid / Relevant description / Source or reference
Strains
P.multocida
VP161 / Serotype A:1, Vietnamese chicken isolate / (40)
AL435 / VP161 carrying a Tn916 insertion in gene pm1417Fully virulent / (16)
AL436 / pm1982mutant of AL435 / This study
AL657 / pm1980 mutant of AL435 / This study
AL970 / pm0527 mutant of AL435 / This study
AL1036 / AL970containing pAL544 / This study
AL1037 / AL970containing pAL99 / This study
AL1039 / AL657containing pAL545 / This study
AL1038 / AL657containing pAL99 / This study
E.coli
DH5α / deoR, endA1, gyrA96, hsdR17(rk- mk+), recA1, relA1, supE44, thi-1, (lacZYA-argFV169), Φ80lacZ ΔM15, F- / Bethesda Research Laboratories
Sm10 pir / Strain for propagation of pUA826 and its derivatives / (29)
Plasmids
pUA826 / Mob+, R6K replicon, ApR StrR SpcR. Single-crossover insertional mutagenesis vector / (10)
pAL99 / P.multocida expression plasmid (KanR)
Constitutive tpiA promoter upstream of BamHI / (18)
pAL243 / Internal section of pm1982gene cloned into pUA826 / This study
pAL310 / Internal section of pm1980gene cloned into pUA826 / This study
pAL528 / Internal section of pm0527gene cloned into pUA826 / This study
pAL544 / Complete pm0527gene cloned into pAL99 / This study
pAL545 / Complete pm1980gene cloned into pAL99 / This study

SupplementalTable S2. Oligonucleotides used in this study.

Oligo / Sequence / Description
BAP2371 / AACTTGAATTCGTGAACCCATTGATTCAGGG / pm1982 internal forward primer for mutagenesis
BAP2372 / ATTTTGAATTCTAAATGGCATTGAACCGTCA / pm1982 internal reverse primer for mutagenesis
BAP2596 / CACCATGGCTAATCTGGATGATTCTTATC / pm0527 flanking forward primer for expression
BAP2597 / TTTTGCTGAGTAATACCCTGCCA / pm0527 flanking reverse primer for expression
BAP2782 / GCCCTACACAAATTGGGAGA / pUA826 primer near SalI site
BAP3466 / GTAAAAGTCGACAAGCACAAGCAATCGTGAA / pm1980 internal forward primer for mutagenesis
BAP3467 / TTGAATGTCGACACGCTGATGCAGTAAA / pm1980 internal reverse primer for mutagenesis
BAP3578 / AAGTGGTCCTGCAACTTTATCCG / pUA826 primer near BamHI site
BAP4368 / AAGTTGGTCGACGCTATATCATGATAC / pm0527 internal forward primer for mutagenesis
BAP4370 / CTGACGAGTCGACTAGCCATAGGGGTATCTA / pm0527 internal reverse primer for mutagenesis
BAP4650 / AAATTTGTCGACGCTTCTTTCCACTTCT / pm0527 flanking forward primer for expression (complementation)
BAP4651 / CTCTCCGTCGACGGCGCAACGAATAGGAGGGCGTAA / pm0527 flanking reverse primer for expression (complementation)
BAP4863 / TGAAAAGTCGACCCAGCAAAATGAAGAGCAA / pm1980 flanking forward primer for expression (complementation)
BAP4864 / TTACGGTCGACAGCGAAAATTGAAGTGAA / pm1980 flanking reverse primer for expression (complementation)

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