Supplementary Table 1 The analysis of positional differences in the gyrB sequences of 1.2kb a

Species (or serotype) / Species-specific position
Escherichia coli r / Subgroup I k: C/T (519), C/A (1002)
Subgroup II q: c —
O157: T (1069)
Shigella boydii / —
Shigella flexneri / C/T (1353) p
Shigella dysenteriae / —
Shigella sonnei / C/T (924) p, G/A (1041) p
Salmonella Choleraesuis / C/T(528), T/C(780), T/C(840), 888(C/T), C/T(903), C/T(918), C/T(930), C/T(939), A/G(1341)
Salmonella Enteritidis / C/T(795), C/T(828), C/T(933), A/G(1002), T/C(1014), C/T(1081)
Salmonella Paratyphi A / C/T(369), G/A(672), C/T(711), C/T(735), G/A(754) Val-Ile g, C/T(843)
Salmonella Paratyphi B j / C/T (612), C/T( 627), G/A(693), T/C(696), C/T(996)
Salmonella Typhi / A/G(393), A/G(981)
Salmonella Typhimurium / —
Aeromonas caviae d, l / C/G(660) m, T/G(873), T/G(876), T/G(1047) n
Aeromonas hydrophila d, s / Subgroup Ie: C/T(477), AG/GC(478~479) Ser/Ala g, GG/AC(484~485) Gly/Thr g, G/T(828)
Subgroup II f: G/C (828)
Aeromonas veronii d, h, i / A/G (520), G/A (1011), G/A (1161)
Vibrio cholerae / b…
Vibrio parahaemolyticus / …

a The positional sequence differences of Yersinia enterocolitica and Plesiomonas shigelloides were excluded from this analysis for their small sample sizes.

b …There are too many species-specific differences to be listed.

c — No species-specific variations for this species.

d In the process of finding the specific variations of Aeromonas spp., a complete list of accession numbers is available on request.

e A. trota and A. schubertii share some of these specific differences. The differences that differentiate A. hydrophila Subgroup I with these two species were not listed.

f A. jandaei share some of these specific differences. The differences that differentiate A. hydrophila Subgroup II with A. jandaei were not listed.

g Amino acid alteration due to the corresponding non-synonymous substitution.

h Not possessed by all A. veronii sequences.

i A. hydrophila strains CC11 and TI14 were included.

j The positional differences are possessed by S. Paratyphi B sequences except ATCC 8759.

k E. coli subgroup I included ATCC11775, ATCC25922, and three uropathogenic E. coli (UPEC) strains ( CFT073, 536, and UTI89). In the phylogenetic tree, these strains formed a distinct monophyletic subgroup.

l A. hydrophila strain CI76-15T was included.

m A. hydrophila strain P3 was included.

n A. veronii strain MDC260 was included.

p Signatures only suitable for identification among different Shigella species

q E. coli subgroup II is composed of strains that mixed with Shigella in the phylogentic tree.

r In the process of finding the specific variations of E. coli, a complete list of accession numbers is available on request.

s A. hydrophila subgroup I only contained our native sequences and ICB sequences; subgroup II contained 10 European isolates with only two Chinese and Japanese strains.

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