Online Resource 3
Supplementary Table 1
Table S1 Comparison between properties of β-glucosidases from different microorganisms
Microrganism / Mr / Quaternary / Optimum / Optimum / Kma / Vmaxa / Reference(kDa) / structure / pH / temperature (℃) / (mM) / (U/mg)
Fusarium oxysporum / 110 / Monomer / 4.8 / 60 / 0.09 / 16.4 / Christakopoulos et al. 1994
Candida peltata / 43 / Monomer / 5.0 / 50 / 2.30 / 221.0 / Saha and Bothast 1996
Aspergillus nidulans / 105 / Dimmer / 5.0 / 55 / 21.70 / 199.0 / Yan and Lin 1997
Aspergillus oryzae / 40 / Monomer / 5.0 / 50 / 0.55 / 3,040.0 / Riou et al. 1998
Daldinia eschscholzii / 64 / Monomer / 5.0 / 50 / 1.52 / 3.2 / Karnchanatat et al. 2007
Penicillum brasilianumc / 115 / NRb / 5.0-6.0 / 60 / 0.09 / 76.0 / Krogh et al. 2010
Humicola grisea var. thermoidea / 60 / Monomer / 6.0 / 50 / 0.12 / 41.3 / Nascimento et al. 2010
Penicillum purpurogenum KJS506 / 110 / Monomer / 5.0 / 50 / 5.10 / 934.0 / Jeya et al. 2010
Neotermes koshunensisc / 60 / Monomer / 5.0 / 50 / 0.77 / 16.0 / This work
a Kinetic parameters using pNPG as substrate
b NR = not reported by authors
c Recombinant protein
References
Christakopoulos P, Goodenough PW, Kekos D, Macris BJ, Claeyssens M, Bhat MK (1994) Purification and characterisation of an extracellular β-glucosidase with transglycosylation and exo-glucosidase activities from Fusarium oxysporum. Eur J Biochem 224:379-385
Jeya M, Joo AR, Lee KM, Tiwari MK, Lee KM, Kim SH, Lee JK (2010) Characterization of β-glucosidase from a strain of Penicillium purpurogenum KJS506. Appl Microbiol Biotechnol 86:1473-1484
Karnchanatat A, Petsom A, Sangvanich P, Piaphukiew J, Whalley AJ, Reynolds CD, Sihanonth P (2007) Purification and biochemical characterization of an extracellular β-glucosidase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm. FEMS Microbiol Lett 270:162-170
Krogh KB, Harris PV, Olsen CL, Johansen KS, Hojer-Pedersen J, Borjesson J, Olsson L (2010). Characterization and kinetic analysis of a thermostable GH3 β-glucosidase from Penicillium brasilianum. Appl Microbiol Biotechnol 86:143-154
Nascimento CV, Souza FH, Masui DC, Leone FA, Peralta RM, Jorge JA, Furriel RP (2010) Purification and biochemical properties of a glucose-stimulated β-D-glucosidase produced by Humicola grisea var. thermoidea grown on sugarcane bagasse. J Microbiol 48:53-62
Riou C, Salmon JM, Vallier MJ, Günata Z, Barre P (1998) Purification, characterization, and substrate specificity of a novel highly glucose-tolerant β-glucosidase from Aspergillus oryzae. Appl Environ Microbiol 64:3607-3614
Saha BC, Bothast RJ (1996) Production, purification, and characterization of a highly glucose-tolerant novel β-glucosidase from Candida peltata. Appl Environ Microbiol 62:3165-3170
Yan TR, Lin CL (1997) Purification and characterization of a glucose-tolerant β-glucosidase from Aspergillus niger CCRC 31494. Biosci Biotechnol Biochem 61:965-970