Supplementary Table 1 Infection types of Baidatou, Mingxian 169, Fielder, Tres, Tyee and Lee to prevalent P. striiformis tritici races at the seedling and adult-plant stages

Race / Cultivar / Seedling / Adult
Greenhouse / Field
CYR 25 / Baidatou / 4 / 0; / 1
Mingxian 169 / 4 / 4 / 4
CYR 27 / Baidatou / 3 / 0; / 2
Mingxian 169 / 4 / 4 / 4
CYR 29 / Baidatou / 3 / 0; / 0;
Mingxian 169 / 4 / 4 / 4
CYR 30 / Baidatou / 3+ / 0; / 0;
Mingxian 169 / 4 / 4 / 4
CYR 31 / Baidatou / 3 / 0; / 0;
Mingxian 169 / 4 / 4 / 4
CYR 33 / Baidatou / 3+ / 0; / 0;
Mingxian 169 / 4 / 4 / 4
CYR 32 / Baidatou / 3+ / 0; / 0;
Mingxian 169 / 4 / 4 / 4
Sull-4 / Baidatou / 3+ / 0; / 0;
Mingxian 169 / 4 / 4 / 4
CYR 33 / Baidatou / 3+ / 0; / 0;
Mingxian 169 / 4 / 4 / 4
Fielder / 3 / 4 / 3
Tres / 3 / 4 / 3
Tyee / 4 / 4 / 4
Lee / 4 / 4 / 4

Supplementary Table 2 Amplification result of SSR and SRAP markers in the F3 population from Mingxian 169/Baidatou

Marker / RP allele (bp)a / SP allele (bp) / CS allele / No. of Markers in / χ2 / Pvalue
Ab / H / B
Xgwm132 / 150 / 140 / + / 33 / 86 / 36 / 1.98 / 0.37
Xbarc54 / 180 / 190 / + / 34 / 80 / 41 / 0.79 / 0.67
Xcfd19 / 420 / 440 / + / 39 / 81 / 35 / 0.52 / 0.77
Me8-Em1 / - / 100 / - / 121 / 0 / 34 / 0.62 / 0.38
Me4-Em5 / - / 135 / - / 119 / 0 / 36 / 0.17 / 0.61
Xbarc416 / 265 / - / + / 33 / 84 / 38 / 1.41 / 0.49

a Fragment size was visually estimated based on the 100-bp DNA ladder

b A, resistant parent allele; H, both resistant and susceptible parent allele; B, susceptible parent allele

Pp: resistance parent; Sp: susceptible parent; CS: Chinese Spring.

Supplementary Table 3 Polymorphisms of molecular markers flanking the YrBai locus in 99 wheat genotypes

Cultivar / Xcfd19 / Xbarc54 / Cultivar / Xcfd19 / Xbarc54 / Cultivar / Xcfd19 / Xbarc54
Baidatou / +a / + / Jintai 170 / - / - / Shi 4185 / - / -
Mingxian 169 / - / - / An 0817 / - / - / Tian 94-3 / - / -
Zhongyuan 6 / - / - / Yan 4110 / - / - / Tian 98101 / - / -
Zhengmai 7698 / - / - / Jimai 6097 / + / - / Zhongliang 9589 / - / -
Jinhe 9123 / - / + / Kenong 1006 / - / - / Zhongliang 30 / - / -
wanke06290 / - / - / Heng 4422 / - / - / Tianxuan 45 / + / -
Xiannong 1 / - / - / Shanong 055849 / - / - / Zheng 9023 / - / -
Huaimai 0705 / - / + / Tainong 8681 / - / - / Xiaoyan 9323 / - / -
Wanke 700 / - / - / Heng 5364 / - / - / Jimai 37 / - / -
Change 25 / - / - / Shi B08-5341 / - / + / Lantian 10 / - / -
Bainong 207 / - / - / Fengdecunmai / - / - / Jimai 47 / - / -
Annong 0822 / - / + / Shi 4185 / - / - / Xinong 291 / - / +
Zhoumai 28 / - / - / Liangxing 66 / - / - / Xinong 65 / + / -
Zhoumai 18 / - / - / Shannong 055843 / - / - / Lantian 18 / - / -
Bomai 8901 / + / - / Shi 06-6136 / - / - / Mian 2000-34 / - / -
Zhoumai 27 / + / - / Zao 9919 / - / - / Shan 876 / - / -
Junmai 35 / - / - / Han 05-5093 / - / - / Shanmai 107 / - / -
Mingtian 0417 / - / + / Wenhang 6 / + / + / Yumai 13 / - / -
PingAn 8 / + / - / Heng 07-5114 / - / + / Shan 481 / + / -
Womai 0608 / + / + / Shi H083-366 / - / - / Han 894 / - / -
Danshi 802 / + / + / Jimai 112 / - / - / Xinong 129 / - / -
Huahui 1088 / - / - / Chuanmai 107 / - / - / Shannong 28 / - / -
Zhongfan 5 / + / - / Henong 130-12 / - / + / Xike 61015 / + / -
Zhongmai 895 / + / + / Jinmai 38 / - / - / Yan 9201 / + / -
Huaimai 0615 / + / - / Tai 13606 / - / - / Zhengmai 9023 / - / -
Xinong 979 / - / - / Chang 8255 / - / - / Zhoumai 12 / - / -
Luo 3429 / + / + / Hangmai 309 / - / - / Yannong 15 / - / -
Tianmin 198 / - / + / Zhongmai 861 / - / - / Lumai 23 / - / -
Huaimai 05155 / + / + / Jin 07006 / - / - / Jingdong 8 / - / -
Mingtian 07112 / - / + / Zhongyou 629 / - / - / Longyuan 935 / - / -
Zhoumai 30 / - / - / Jingmai 9 / - / - / Emai 14 / - / -
Xinmai 23 / - / + / Zhongmai 17 / - / - / Yumai 49 / - / -
Womai 8 / - / + / Tianxuan 43 / - / - / Fanmai 8 / - / +
Luomai 24 / - / - / Tianxuan 46 / - / -

a Presence of marker (+). Absence of marker (-)

Supplementary Fig. 1 PCR profile of Xcfd19 (A) and Xbarc54 (B) in F2:3 lines from the cross of Mingxian 169/Baidatou.

M: DNA ladder; PR: Baidatou; Ps: Mingxian 169; BR: resistant pool; BS: susceptible pool; R: homozygously resistant F2:3 individual plant; H: heterozygous resistant individual plant; S: susceptible F2:3 individual plant.