Table S1. B. subtilis strains

Strain / Genotypea / Reference/source/constructionb,c,d
SU5 / 168 trpC2 / E. Nester
SU110 / 168 thyA thyB trpC2 / Reference 28
SU111 / SU110 ftsZ(ts1) / Reference 28
SU492 / SU5 amyE::Pxyl-ftsZ-yfp (spec) / Reference 31
SU558 / SU5 amyE::Pspachy-ftsZ (neo) / PL950  SU5
SU561 / SU5 ezrA::neo / Reference 29
SU576 / SU110 ftsZ(ts1) pHV1249 (cat, erm) / pHV1249  SU111
SU592 / SU110 pyk::pMutinT3 (erm) ftsZ(ts1) / See Materials and Methods
SU610 / SU110 pyk::pMutinT3 (erm) amyE::Pxyl-pyk (spec) ftsZ(ts1) / See Materials and Methods
SU664 / SU5 pyk::cat / See Materials and Methods
SU679 / SU5 pyk::catamyE::Pxyl-ftsZ-yfp (spec) / SU664  SU492
SU696 / SU5 pyk::cat amyE::Pspachy-ftsZ (neo) / SU664  SU558
SU699 / SU5 pyk::pyk-gfp (cat) / See Materials and Methods
SU702 / SU110 pyk::catftsZ(ts1) / SU664  SU111
SU703 / SU110 citC::spec ftsZ(ts1) / DGRM32  SU111
SU704 / SU110 citH::spec ftsZ(ts1) / DGRM33  SU111
SU705 / SU110 pgkEP araE::spec ftsZ(ts1) / DGRM18  SU111
SU706 / SU110 pycA::erm ftsZ(ts1) / DRGM38  SU111
SU708 / SU110 gapB::pMutin2 (erm) ftsZ(ts1) / DGRM36  SU111
SU709 / SU110 pdhC::neo ftsZ(ts1) / DGRM28  SU111
SU710 / SU110 pdhD::neo ftsZ(ts1) / DGRM29  SU111
SU713 / SU110 pckA::spec ftsZ(ts1) / DGRM37  SU111
SU714 / SU110 pgi::spec ftsZ(ts1) / DGRM15  SU111
SU739 / SU5 amyE::Pxyl-pdhA-yfp (spec) / See Materials and Methods
SU741 / SU5 amyE::Pxyl-pdhA (spec) / See Materials and Methods
SU742 / SU5 pyk::catamyE::Pxyl-pdhA-yfp (spec) / SU739  SU664
SU743 / SU5 pyk::catamyE::Pxyl-pdhA (spec) / SU741  SU664
SU762 / SU110 pgcA::spec ftsZ(ts1) / PL2281  SU111
SU763 / SU110 gtaB::spec ftsZ(ts1) / PL2357  SU111
SU764 / SU110 ugtP::cat erm ftsZ(ts1) / PL2236  SU111
SU791 / SU5 pdhA::catamyE::Pxyl-pdhA (spec) / See Materials and Methods
SU792 / SU5 ezrA::neopdhA::catamyE::Pxyl-pdhA (spec) / SU561  SU791
DGRM15 / 168 pgi::spec trpC2 / Reference 67
DGRM18 / 168 pgkEP araE::spec trpC2 / Reference 67
DGRM28 / 168 pdhC::neo trpC2 / Reference 67
DGRM29 / 168 pdhD::neo trpC2 / Reference 67
DGRM32 / 168 citC::spec trpC2 / Reference 67
DGRM33 / 168 citH::spec trpC2 / Reference 67
DGRM36 / 168 gapB::pMutin2 (erm) trpC2 / Reference 67
DGRM37 / 168 pckA::spec trpC2 / Reference 67
DGRM38 / 168 pycA::erm trpC2 / Reference 67
PL950 / JH642 amyE::Pspachy-ftsZ (neo) / Reference 37
PL2236 / JH642 ugtP::cat erm / Reference 24
PL2357 / JH642 gtaB::spec / Reference 24
PL2281 / JH642 pgcA::spec / Reference 24

a Antibiotic resistance genes are expressed as follows: cat, chloramphenicol; erm, erythromycin; neo, neomycin; spec, spectinomycin; tet, tetracycline.

b Arrows indicate construction by transformation (donor DNA  recipient strain).

c References are listed in the main text.

dFor details of metabolic mutant strains DGRM15-38 and their construction, see reference 67 and references therein. Notably, the pgkEP mutation is a single base pair deletion that truncates the pgk gene at codon 51 (out of 394). All other genes have been deleted or disrupted by insertion of an antibiotic resistance marker.