Supplemental Figure

Fig.S1RT-PCR analysis of the gene expression of BC-MSCs and BM-MSCs afterspecificlineage differentiation

(A)Specific gene expression for adipogenesis and osteogenesisof BC-MSCswere analyzed by RT-PCR, the specific expression of genesfor adipogenesis werePPAR-γ2, adipsin and LPL) and the specific genes for osteogenesis were osteopontin(OPN) and alkaline phosphatase (ALP). The left lane indicatedthe gene expression of non-induced BC-MSCs; the right lane showed the gene expression of induced BC-MSCs.(B) BM-MSCs were also induced under specific medium as a positive control group, thespecific gene expression for adipogenesis and osteogenesiswereevaluatedby RT-PCR analysis.

Fig.S2EGF couldpromote MCF7 mammosphere formation

EGF couldpromote MCF7 mammosphere formation than bFGF, when co-cultured with BC-MSCs, the EGF adding group showed more mammospheres formation than the bFGF adding group.

Fig.S3 BC-MSCs promoted breast cancer cell migration in vitro

The effect of BC-MSC’s conditional medium on migration of breast cancer cell MCF7 and SK-BR-3. A representative result from at least three separate experiments was shown. Photograph shown was a representative field stained by DAPI. ×10.

Table S1.Primer sequences for reverse transcription–polymerase chain reactions.

gene / Primer sequence
GAPDH / Sense / GAGTCAACGGATTTGGTCGT
Antisense / TTGATTTTGGAGGGATCTCG
Vimentin / Sense / GAGAACTTTGCCGTTGAAGC
Antisense / GCTTCCTGTAGGTGGCAATC
N-cadherin / Sense / ACAGTGGCCACCTACAAAGG
Antisense / CCGAGATGGGGTTGATAATG
E-cadherin / Sense / TGCCCAGAAAATGAAAAAGG
Antisense / GTGTATGTGGCAATGCGTTC
a-SMA / Sense / CAGGGCTGTTTTCCCATCCAT
Antisense / GCCATGTTCTATCGGGTACTTC
CollagenⅠ / Sense / GTCGAGGGCCAAGACGAAG
Antisense / CAGATCACGTCATCGCACAAC
Fibronectin / Sense / GAAGCTCTCTCTCAGACAACCA
Antisense / GCCCACGGTAACAACCTCTT
CK8 / Sense / CCTGGAAGGGCTGACCGACGAGATCAA
Antisense / CTTCCCAGCCAGGCTCTGCAGCTCC
CK18 / Sense / AGCTCAACGGGATCCTGCTGCACCTTG
Antisense / CACTATCCGGCGGGTGGTGGTCTTTTG
PPAR-γ2 / Sense / TGTCAGTACTGTCGGTTTC
Antisense / AATGGTGATTTGTCTGTTG
Adipsin / Sense / GGTCACCCAAGCAACAAAGT
Antisense / CCTCCTGCGTTCAAGTCATC
LPL / Sense / GTCCGTGGCTACCTGTCAT
Antisense / AGCCCTTTCTCAAAGGCTTC
ALP / Sense / GCTTGACCTCCTCGGAAGACACTC
Antisense / CGCCTGGTAGTTGTTGTGAGCATAG
Osteopontin / Sense / GTGCCATACCAGTTAAACA
Antisense / CTTACTTGGAAGGGTCTCT
EGF / Sense / AAGGTACTCTCGCAGGAAATGG
Antisense / ACATACTCTCTCTTGCCTTGACC
bFGF / Sense / AGAAGAGCGACCCTCACATCA
Antisense / ACTGCCCAGTTCGTTTCAGTG
CCL5 / Sense / TGCAGAGGATCAAGACAGCA
Antisense / GAGCACTTGCCACTGGTGTA
Bmp4 / Sense / GGAAGAAGAATAAGAACTGCC
Antisense / CCACCTTATCATACTCATCCA
HGF / Sense / ACGAACACAGCTATCGGGGTA
Antisense / CATCAAAGCCCTTGTCGGGAT
Gremlin1 / Sense / GCACAATGACTCAGAGCAGAC
Antisense / GCTTCAGGTATTTGCGCTCC
IL-6 / Sense / GGAAGGTTCAGGTTGTTTTCTGC
Antisense / AAATTCGGTACATCCTCGACGG
SDF1a / Sense / GCCGGGCTACAATCTGAAGG
Antisense / ATGCCCATGCCGATTCTTCG
IGF-II / Sense / TTGCTCTACCCACCCAAGAC
Antisense / GATGGAACCTGATGGAAACG
IGF-I / Sense / GCTGGTGGATGCTCTTCAGTTC
Antisense / AGCTGACTTGGCAGGCTTGAG
TGF-β1 / Sense / GCGTGCTAATGGTGGAAAC
Antisense / GCTGAGGTATCGCCAGGAAT
HIF-1a / Sense / GGCGCGAACGACAAGAAAAAG
Antisense / CCTTATCAAGATGCGAACTCACA
CTGF / Sense / AACACCATAGGTAGAATGTAAAGC
Antisense / CTGATCAGCTATATAGAGTCACTC