Supplementary Table 2

Crayfish plague events in Estonia during 2006–2015

Sample origina / Crayfish speciesb / Sampling year / No and status of analysed individualsc / PCR/ qPCR resultd / Highest agent levele / Genotype groupf / Analysing placeg / Analysing year
Pihtla crayfish farm, Island Saaremaa, OB in 2006 / A.a / 2006 / 2 alive* / 2 posit / A3 / NT / NVI / 2007
2006 / 2 alive / 2 posit / A2 / NT / Univ-Poitiers / 2016
Pähkla fish and crayfish farm, Island Saaremaa, OB in 2007 / A.a. / 2007 / 1 dead* / 1 posit / A7 / NT / NVI / 2007
Põduste River, Island Saaremaa, OB in 2007, CE in 2008 / A.a. / 2007 / 11 dead* / 8 posit / A7 / NT / NVI / 2007
2008 / 1 alive / 1 posit / A1 / NT / Univ-Poitiers / 2016
Laugi Stream, Island Saaremaa, OB in 2007 / A.a. / 2007 / 4 alive* / 2 posit / A6 / NT / NVI / 2007
2007 / 1 dead / 1 posit / A3* / E / Univ-Poitiers / 2016
Sõrve crayfish farm, Island Saaremaa, OB in 2007 / A.a. / 2007 / 2 alive* / 1 posit / A2 / NT / NVI / 2007
Riksu Stream, Island Saaremaa / P.l. / 2011 / 4 alive / 3 posit / NK / NT / EULS / 2011
2011 / 2 alive** / 2 posit / A3 / NT / Univ-Poitiers / 2014
2013 / 5 alive / 1 posit / A2 / NT / Univ-Poitiers / 2014
2015 / 6 alive / 4 posit / A2 / NT / Univ-Poitiers / 2015
Masa River, Island Saaremaa, OB in 2013 / A.a. / 2013 / 4 dead / 1 posit / A2 / NT / Univ-Poitiers / 2014
2013 / 1 alive / 1 posit / A2 / NT / Univ-Poitiers / 2016
Leevi River, Põlva, OB in 2007, CE in 2009 / A.a. / 2007 / 2 dead* / 2 posit / A5 / NT / NVI / 2007
2007 / 2 dead / 2 neg / A0 / NT / Univ-Poitiers / 2016
2009 / 1 died*, 1 alive* / 2 posit* / NK / NT / EVIRA / 2009
Mustjõgi River, Võru, DD in 2008 / A.a. / 2008 / 1 alive* / 1 posit* / NK / NT / EVIRA / 2009
Mustjõgi River, Harju, CE in 2009 / A.a. / 2009 / 2 dead* / 2 posit* / NK / NT / EVIRA / 2009
P.l. / 2009 / 1 alive* / 1 neg* / NK / NT / EVIRA / 2009
Lake Selja, West-Viru, OB in 2009 / A.a. / 2009 / 3 dead / 3 posit* / NK / NT / EVIRA / 2009
2009 / 2 dead / 2 posit / A1 / NT / Univ-Poitiers / 2016
Pärlijõgi River, Võru, OB in 2008-2010 / A.a. / 2010 / 4 dead / 3 posit* / NK / NT / EVIRA / 2010
2010 / 1 dead / 1 posit / NK / NT / EULS / 2010
2010 / 1 dead / 1 posit / A4* / B / Univ-Poitiers / 2014
2010 / 2 dead / 2 posit / A5* / B / Univ-Poitiers / 2016
Härjanurme fish and crayfish farm, Jõgeva, OB in 2010 / A.a. / 2010 / 1 dead / 1 posit / NK / NT / EULS / 2010
2010 / 1 dead / 1 posit / A4* / A / Univ-Poitiers / 2016
Taebla River, West, DD in 2010 / A.a. / 2011 / 1 alive / 1 posit / NK / NT / EULS / 2011
2011 / 1 alive** / 1 posit / A3 / NT / Univ-Poitiers / 2014
Vääna River, Harju / P.l. / 2015 / 4 alive / 2 posit / A1 / NT / Univ-Poitiers / 2015
A.a. / 2015 / 2 alive / 2 neg / A0 / NT / Univ-Poitiers / 2015
Avijõgi River, East-Viru, OB in 2015 / A.a. / 2015 / 3 dead / 3 posit / A4* / A / Univ-Poitiers / 2015

a Sample origin: All samples originate from suspected incidences of crayfish plague in Estonia. The sample origin listed includes the name of population (farm, lake, river or stream) and county. The crayfish samples originate either outbreak (OB), decline of density (DD) or cage experiment (CE) that has been used for crayfish plague surveillance in the years after an outbreak.

b A.a. = Astacus astacus; P.l. = Pacifastacus leniusculus.

c*Crayfish tissue material or DNA-isolates were not stored, **used DNA in analyses was extracted in Estonian University of Life Sciences (EULS) in 2011.

d *Analyses in Finnish Food Safety Authority (EVIRA) based on Oidtmann et al. (2004) PCR methodology. Others based on Vrålstad et al. (2009) qPCR methodology.

eOnly the highest detected agent level is given. Results marked with * have been confirmed by sequencing. NK = not known – the agent level was not included to the protocol.

f Genotype group determined based on microsatellite markers. NT = not tested.

gNVI = Norwegian Veterinary Institute; EVIRA; Univ-Poitiers = University of Poitiers; EULS.