Tentative Lab Schedule and Supply List
Bio 211 (formerly 201); Fall 2008
Week 1: Microscopy
o “e”’s for prep of wet mount
o Clean slides
o Coverslips
o Lens cleaner and lens paper
o Water bottles (droppers)
o Thread Slides
o Pond Water
Week 2: Cell Diversity
o Bacterial shape slides
o Dilute Yogurt
o Immersion oil, lens cleaner, lens paper
o Live cyanobacteria (if available)
o Sterile toothpicks
o Methylene blue dropper bottles
o Water dropper bottles
o Clean Slides
o Coverslips
o Lens Cleaner and lens paper
o Biohazard bucket for toothpicks and coverslips
o Biohazard dish for used cheek slides
o Elodea
o Potato
o Carrot or Red Pepper
o Razorblades
o Lugol’s solution dropper bottles
o Kimwipes
Week 3: Membrane Physiology
o Starch Dialysis Slide Demo
o Eggs for diffusion study (2 per group + unknown solutions)
o Balances
o Weigh boats
o Kimwipes
o Clean slides and coverslips
o Elodea
o Water dropper bottles
o Concentrated salt dropper bottles
o Optional: Agar Plate Dye Diffusion Demo
Week 4: Enzyme Function
o Test Tubes (17/group)
o Test Tube Rack (1/group)
o Parafilm
o Pipets
o 250 ml beaker
o pH 7 Phosphate Buffer
o 50 C Water Bath
o Catechol with pipets
o Ice
o Potato Extract with pipets
o Spectrophotometers
o Kimwipes
o Dilute NaOH and HCl in dropper bottles
o pH Paper
Week 5: Respiration and Photosynthesis
o 4 large clean test tubes per group
o pH meters
o distilled water squirt bottles
o ~400ml beakers for rinsing (1 per group)
o Wax pencils and/or tape and pens
o Healthy Elodea
o Well water
o Balances and weigh boats
o Light Table or Lights
Week 6: Film- No supplies.
Week 7: GM Investigator, Part 1
o Biorad Kits: GMO Investigator 166-2500EDU
o Mortar and pestle (1 per group if possible)
o Aliquoted InstaGene Matrix (shake well and continuously while pipetting!)
o 100 C water bath
o Microcentrifuge
o Aliquoted GMO primer master mix (on ice)
o Aliquoted plant primer master mix (on ice)
o Thermocycler
o Micropipettes
o Clean tips
o Practice solutions (food coloring)
Week 8: GM Investigator, Part 2
o Melted Agarose Solution (from kit)
o Electrophoresis chambers and power supplies
o Electrophoresis buffer
o 100X Fast Blast Stain
o Micropipettes and tips
o Gel camera
Week 9: Mitosis, Meiosis, and Inheritance
o Onion root tip mitosis slides
o Whitefish blastula mitosis slides
o Lillium anthers and ovary meiosis slides
o Beads for modeling mitosis and meiosis
o Mitosis and Meiosis posters
Week 10: pGLO, Part 1
o pGLO Kits: Biorad 166-0003EDU
o Ice Buckets/baths
o Heat Block
o Agar Plates (1 LB, 2 LB+amp, 1 LB+amp+ara/group)
o 37 C Incubator
o UV boxes
o Biohazard Set-up
Week 11: pGLO, Part 2 (analysis of results)
o UV boxes
o Biohazard Set-up
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