Tentative Lab Schedule and Supply List

Bio 211 (formerly 201); Fall 2008

Week 1: Microscopy

o  “e”’s for prep of wet mount

o  Clean slides

o  Coverslips

o  Lens cleaner and lens paper

o  Water bottles (droppers)

o  Thread Slides

o  Pond Water

Week 2: Cell Diversity

o  Bacterial shape slides

o  Dilute Yogurt

o  Immersion oil, lens cleaner, lens paper

Live cyanobacteria (if available)

o  Sterile toothpicks

o  Methylene blue dropper bottles

o  Water dropper bottles

o  Clean Slides

o  Coverslips

o  Lens Cleaner and lens paper

o  Biohazard bucket for toothpicks and coverslips

o  Biohazard dish for used cheek slides

o  Elodea

o  Potato

Carrot or Red Pepper

o  Razorblades

o  Lugol’s solution dropper bottles

o  Kimwipes

Week 3: Membrane Physiology

o  Starch Dialysis Slide Demo

Eggs for diffusion study (2 per group + unknown solutions)

o  Balances

o  Weigh boats

o  Kimwipes

o  Clean slides and coverslips

o  Elodea

o  Water dropper bottles

o  Concentrated salt dropper bottles

o  Optional: Agar Plate Dye Diffusion Demo

Week 4: Enzyme Function

o  Test Tubes (17/group)

o  Test Tube Rack (1/group)

o  Parafilm

o  Pipets

o  250 ml beaker

o  pH 7 Phosphate Buffer

o  50 C Water Bath

o  Catechol with pipets

o  Ice

o  Potato Extract with pipets

o  Spectrophotometers

o  Kimwipes

o  Dilute NaOH and HCl in dropper bottles

o  pH Paper

Week 5: Respiration and Photosynthesis

o  4 large clean test tubes per group

o  pH meters

o  distilled water squirt bottles

o  ~400ml beakers for rinsing (1 per group)

o  Wax pencils and/or tape and pens

o  Healthy Elodea

o  Well water

o  Balances and weigh boats

o  Light Table or Lights

Week 6: Film- No supplies.

Week 7: GM Investigator, Part 1

Biorad Kits: GMO Investigator 166-2500EDU

o  Mortar and pestle (1 per group if possible)

o  Aliquoted InstaGene Matrix (shake well and continuously while pipetting!)

o  100 C water bath

o  Microcentrifuge

o  Aliquoted GMO primer master mix (on ice)

o  Aliquoted plant primer master mix (on ice)

o  Thermocycler

o  Micropipettes

o  Clean tips

o  Practice solutions (food coloring)

Week 8: GM Investigator, Part 2

o  Melted Agarose Solution (from kit)

o  Electrophoresis chambers and power supplies

o  Electrophoresis buffer

o  100X Fast Blast Stain

o  Micropipettes and tips

o  Gel camera

Week 9: Mitosis, Meiosis, and Inheritance

o  Onion root tip mitosis slides

o  Whitefish blastula mitosis slides

o  Lillium anthers and ovary meiosis slides

o  Beads for modeling mitosis and meiosis

o  Mitosis and Meiosis posters

Week 10: pGLO, Part 1

o  pGLO Kits: Biorad 166-0003EDU

o  Ice Buckets/baths

o  Heat Block

o  Agar Plates (1 LB, 2 LB+amp, 1 LB+amp+ara/group)

o  37 C Incubator

o  UV boxes

o  Biohazard Set-up

Week 11: pGLO, Part 2 (analysis of results)

o  UV boxes

o  Biohazard Set-up

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