Table S1. Pseudogenes identified in Wgm. Genes were considered pseudogenes if they were less than half the length of their functional homolog in related speciesdue to a premature stop codon or a missing start codon. Genes are organized by function. Gene status shows the types of mutations that result in a pseudogene. Number of nucleotides that generate the frameshift are shown in parentheses. Positions are indicated as nucleotide position after the start codon. nt=length in nucleotides,aa=amino acid length.

Gene Function / W. g. brevipalpis / W. g. morsitans
Cell processes: 3 / Gene Status / GC% / Gene Status / GC %
grpE / 672 nt, 224 aa / 21.13 / 672 nt, frameshift (-1) at 176 within multi-A stretch; 1st part aligns poorly / 23.70
ftsK / 495 nt, 165 aa / 15.96 / 246nt, frameshift (-2) @7T,9A then to 507 / 15.84
bacA / 804nt, 268 aa / 16% / 270nt, frameshift(-1) to 804 / 20.92
Cell structure: 2
b2511 / 1371bp, 457aa / 19.77 / 740nt,frameshift (-1) then to 1373 / 20.76
pssA / 1368nt, 456 aa / 20.03 / 609nt, frameshift (-4) then to 1367 / 23.48
Information transfer: 2
recJ / 1743nt, 581aa / 24.04 / 387nt, frameshift (-2) then to 1739 / 26.75
nusB / 120nt, (+4) to 423 / 22.67 / 117ntframeshift (-2) then to 423 / 23.44
Metabolism: 2
yqgF / 435nt, 145aa / 26 / no start codon until 201 bp in / 28.89
thiI / no start, stop / n/a / 333nt, 111aa / 20.12
Outer membrane: 1
nlpB / no start / 444 nt (stop codon at 441) / 24.1
Protein binding: 1
ytfN / 2250nt, 750aa / 15.6 / no homologous start codon, 2250nt (from where start would be) / 18.93