Hybrid Proline-Rich Protein

Table S1 Differentially expressed genes in the suppression subtractive hybridization libraries. Genes are listed according to their possible function

Functional category / Annotation of the unigene / Accession number / E-value / Size in base pair
Cell wall
components related / Glycine-rich protein-1 / FK826399 / 1.3e-32 / 410
Germin-like protein / FK826403 / 1.0e-43 / 541
Alpha-expansin 1, complete / FK826411 / 7.7e-22 / 347
Arabinogalactan protein / FK826414 / 2.1e-77 / 619
Arabinogalactan protein / FK826429 / 4.5e-36 / 313
Proline-rich protein / FK826435 / 4.0e-27 / 205
Beta-glucosidase precursor / FK826441 / 3.9e-41 / 575
Beta-glucosidase / FK826475 / 2.6e-22 / 603
Beta-glucosidase precursor / FK826486 / 1.4e-35 / 648
Expansin11 precursor / FK826488 / 1.5e-21 / 309
Xyloglucan endotransglycosylase / FK826511 / 5.2e-36 / 587
Hydroxy proline-rich glycoprotein DZ-HRGP precursor / FK826527 / 2.2e-28 / 516
Pectin methylesterase / FK826535 / 6.8e-33 / 649
Sucrose synthase / FK826549 / 9.1e-17 / 334

Hybrid proline-rich protein

/ FK826602 /

2.2e-70

/

780

Hybrid proline-rich protein

/ FK826615 /

1.9e-19

/

919

P-rich protein

/ GH985159 /

8.4e-40

/

516

Arabinogalactan protein

/ GH985183 /

3.3e-12

/

203

Beta-glucosidase G2

/ GH985185 /

2.0e-50

/

616

Endo-xyloglucan transferase

/ GH985186 /

1.2e-77

/

306

Glycine-rich protein

/ GH985269 /

2.3e-27

/

515

Cellulose synthase catalytic subunit / JK714311 / 1.9e-37 / 679

Proline-rich protein

/ JK743765 / 2.6e-16 / 410
Glycine-rich protein / JK743771 / 1.0e-39 / 435
Cell wall-associated hydrolase / JK743779 / 1.2e-19 / 365
Beta-galactosidase / JK743793 / 8.2e-26 / 517
Pectin methylesterase / JK743804 / 2.3e-51 / 1261 126
Growth and development
Growth and development
(contd.) / Induced stolon tip protein / FK826398 / 8.5e-29 / 494
Always early protein 3 / FK826401 / 2.9e-27 / 624
Ran-binding protein / FK826405 / 5.1e-35 / 599
3-hydroxy-3-methylglutaryl-CoA reductase / FK826413 / 1.8e-31 / 523
Hydrolase / FK826418 / 4.0e-46 / 620
Epicotyl-specific tissue protein / FK826423 / 2.7e-20 / 611
Stearoyl-ACP desaturase / FK826428 / 1.2e-62 / 617
Late embryogenesis-like protein / FK826430 / 8.4e-25 / 359
FH protein interacting protein / FK826438 / 1.9e-62 / 619
Specific tissue protein 2 / FK826446 / 6.7e-19 / 567
Dynein light chain / FK826448 / 1.4e-63 / 537
Spermatid-specific protein T2 / FK826450 / 7.7e-22 / 635

NOI

/ FK826453 /

4.2e-28

/

612

Gip1-like protein, complete / FK826466 / 2.8e-59 / 615
GMFP7 / FK826468 / 2.2e-12 / 248
Nucleic acid binding / FK826483 / 3.6e-30 / 656
Small GTP-binding protein / FK826494 / 5.4e-96 / 625
RAB1C / FK826496 / 9.7e-62 / 421
Peptidyl-prolyl cis-trans isomerase / FK826497 / 1.7e-84 / 625
RAB1Y / FK826500 / 6.9e-30 /

363

FH protein interacting protein / FK826502 / 2.2e-74 / 517

Small GTP-binding protein

/ FK826505 /

2.9e-18

/

193

CLAVATA1 receptor kinase

/ FK826540 / 4.3e-18 / 626
Senescence-associated protein / FK826546 / 4.2e-24 / 339
Ubiquitin OUB2 / FK826547 / 5.9e-25 / 543
Specific tissue protein 2 / FK826550 / 7.3e-21 / 648
Specific tissue protein / FK826551 / 7.7e-12 / 660
Ubiquitin-protein ligase / FK826552 / 1.3e-16 / 593
Ubiquitin ligase SINAT2 / FK826553 / 4.8e-62 / 638
Pectate lyase / FK826563 / 7.8e-66 / 634
Gip1-like protein / FK826564 / 2.8e-104 / 642

Ripening-related protein

/ GH985173 /

3.4e-112

/

623

Specefic tissue protein 2

/ GH985174 /

1.4e-16

/

571

Ubiquitin extensive-like protein

/ GH985175 / 8.4e-17 / 337

RAN

/ GH985192 /

2.2e-19

/

382

axi 1-like protein

/ GH985198 /

1.1e-27

/

620

Ubiquitin-protein ligase

/ GH985214 / 4.2e-28 / 633

Lipoxygenase LOX2

/ GH985221 / 1.6e-50 / 302

COP1-interacting protein 4

/ GH985244 / 5.9e-50 / 647

Gip1-like protein

/ GH985245 / 2.5e-38 / 525

Ubiquitin extension protein

/ GH985256 / 6.8e-33 / 497

Senescence-associated protein

/ JK714290 / 3.3e-30 / 288
Senescence-associated protein / JK714297 / 3.2e-37 / 289
Senescence-associated protein putative / JK714331 / 6.2e-44 / 292
High mobility group protein / JK743770 / 9.5e-45 / 594
Putative senescence-associated protein / JK743791 / 1.0e-37 / 201
Senescence-associated protein / JK743802 / 2.3e-45 / 289
Senescence-associated protein / JK743803 / 1.9e-50 / 221
Senescence-associated protein / JK743808 / 5.0e-48 / 311
Primary metabolism
Primary metabolism (contd.) / Glutathione S-transferase / FK826400 / 2.7e-30 / 377
UDP-D-xylose synthase / FK826459 / 1.2e-18 / 342
Glycosyl transferase family 2 / FK826464 / 9.9e-19 / 310
Hydroquinone glucosyltransferase / FK826473 / 2.1e-89 / 739
Glutamine synthetase / FK826478 / 2.6e-93 / 623
Glyceraldehyde-3-phosphate dehydrogenase / FK826481 / 1.3e-25 / 271
Glycosyl transferase / FK826490 / 1.3e-20 / 306
Glycerol-3-phosphate acyltransferase / FK826503 / 0.87e-33 / 202
ADIPOR-like receptor / FK826512 / 1.5e-90 / 634
ADIPOR-like receptor / FK826524 / 6.5e-29 / 648
Glycosyl transferase family 2 / FK826534 / 2.9e-44 / 304
Beta-1,2-N-acetylglucosaminyltransferase II / FK826561 / 2.3e-99 / 416

Acyl-CoA-binding protein

/ FK826603 /

3.1e-30

/

426

Delta-12 oleate desaturase

/ FK826609 /

3.2e-56

/

415

Delta-12 fatty acid desaturase

/ FK826610 /

8.8e-45

/

206

Digalactosyldiacylglycerol synthase

/ FK826611 /

1.1e-32

/

271

Fatty acid desaturase 2

/ FK826613 /

1.0e-39

/

288

6-phosphofructokinase

/ GH985150 / 1.8e-39 / 271

Oxysterol binding protein

/ GH985182 /

9.5e-20

/

301

Enolase

/ GH985191 /

2.0e-61

/

635

Low molecular weight heat shock protein

/ GH985202 /

8.8e-45

/

447

Glutamine synthetase nodule isozyme

/ GH985211 /

1.1e-32

/

623

Heat shock protein 81-3

/ GH985215 /

8.6e-44

/

625

Fructose-bisphosphate aldolase

/ GH985262 / 1.7e-40 / 636

Omega-3 fatty acid desaturase

/ GH985266 /

4.0e-46

/

531

Triosephosphate isomerase chloroplast precursor / JK714323 / 1.9e-13 / 392
Glutamate decarboxylase putative / JK714325 / 9.6e-36 / 590
Triosephosphate isomerase / JK714330 / 4.3e-66 / 295
Aspartic proteinase nepenthesin-2 precursor / JK743769 / 8.3e-11 / 783
Vicianin hydrolase / JK743785 / 1.7e-38 / 575
Secondary metabolism / Caffeic acid 3-O-methyltransferase / FK826456 / 2.7e-16 / 457
Caffeic acid 3-O methyltransferase / FK826470 / 2.7e-16 / 457
CCR protein / FK826506 / 4.5e-15 / 619

Caffeoyl-CoA-O-methyltransferase

/ FK826614 /

5.4e-61

/

465

4-coumarate:CoA ligase / JK714316 / 1.4e-43 / 586

Inosine monophosphate cyclohydrolase

/ GH985197 /

5.5e-10

/

633

S-adenosylmethionine synthetase 2

/ GH985201 /

1.2e-11

/

172

S-adenosylmethionine synthetase / JK743797 / 1.2e-25 / 649
Methionine synthase / JK743813 / 1.0e-19 / 566
Signalling and gene regulation / Uridylate kinase / FK826406 / 1.5e-59 / 305
Cullin 1C / FK826424 / 2.1e-74 / 602
GTP binding / FK826427 / 1.1e-18 / 604
14-3-3-like protein D / FK826434 / 2.5e-65 / 632
Cyclophilin / FK826457 / 6.2e-48 / 628
14-3-3-like protein B / FK826461 / 1.1e-74 / 654
Catalytic/ protein phosphatase / FK826484 / 9.7e-88 / 723
Transcription factor LIM / FK826415 / 1.2e-10 / 516
Putative calmodulin-binding protein / FK826462 / 8.9e-66 / 654
Calmodulin-binding protein-like / FK826477 / 3.1e-80 / 676
Protein phosphatase 2C-like / FK826517 / 9.1e-19 / 648
Serine/threonine kinase / FK826519 / 1.8e-23 / 643
Uridine monophosphate kinase / FK826523 / 3.5e-17 / 211
Diacylglycerol kinase variant A / FK826565 / 5.7e-17 / 505
Drought-induced protein SDi 6 (fragment) / FK826566 / 9.3e-12 / 411

Epsin

/ GH985156 /

1.3e-15

/

264

Inositol-1,4,5-triphosphate-5-phosphatase

/ GH985172 /

1.2e-35

/

623

GTP-binding protein

/ GH985193 /

8.3e-67

/

263

14-3-3 protein

/ GH985265 /

3.3e-58

/

653

Zinc finger protein

/ JK714288 / 8.9e-24 / 419

Zinc finger protein

/ JK714289 / 5.1e-27 / 419
14-3-3 family protein / JK714308 /

4.7e-75

/

256

14-3-3 family protein / JK714309 / 2.2e-94 /

368

Protein serine/threonine kinase BNK1-like / JK714315 / 3.4e-34 / 400
Cys-3-His zinc finger protein / JK714339 / 3.7e-51 / 407
Calmodulin-7 / JK743796 / 1.1e-47 / 234
MAP kinase kinase kinase Ste11/SteC / JK743805 / 1.8e-37 / 132
70 kDa heat shock cognate protein 2 / JK743811 / 4.2e-56 / 254
Transcription Factor
Transcription Factor
(contd.) / Eukaryotic initiation factor 4A-11 / FK826410 / 7.1e-85 / 565
Homeodomain-leucine zipper protein / FK826421 / 7.3e-21 / 558
DNA-binding protein 4 / FK826431 / 2.8e-29 / 626
Transcription factor / FK826432 / 7.9e-27 / 604
WRKY transcription factor / FK826433 / 7.8e-39 / 620
RNA polymerase transcriptional regulation mediator gene / FK826442 / 1.5e-56 / 620
DNA binding protein / FK826452 / 3.2e-18 / 607
Eukaryotic initiation factor 4A-2 / FK826509 / 7.1e-85 / 627

Homeobox 7

/ GH985231 /

1.3e-93

/

540

Transcriptional regulator / JK743782 / 6.7e-40 / 526
Energy
Energy (contd.) / ATP synthase F0 subunit 9 / FK826408 / 1.4e-28 / 402
Photosystem I reaction center subunit II, chloroplast precursor / FK826417 / 1.2e-11 / 127
Peroxiredoxin Q / FK826420 / 7.8e-66 / 596
Photosystem I subunit XI / FK826422 / 7.7e-12 / 209
Thiazole biosynthetic enzyme / FK826436 / 2.0e-47 / 505
Unspecific monooxygenase / FK826437 / 1.8e-51 / 624
NADH-ubiquinone oxidoreductase / FK826447 / 1.0e-30 / 402
Oxygen evolving complex 33 kDa photosystem II protein / FK826455 / 1.3e-50 / 442
ATP binding / FK826460 / 1.8e-38 / 653
Oxygen-evolving enhancer protein 2-2 / FK826476 / 7.1e-68 / 629
Oxygen-evolving enhancer protein 2-1 / FK826479 / 1.3e-76 / 802
Oxygen evolving enhancer protein 1 / FK826489 / 2.6e-56 / 429
Oxygen evolving enhancer protein 2 / FK826495 / 2.7e-54 / 432
Photosystem II W protein / FK826504 / 3.9e-34 / 455
Formate dehydrogenase / FK826525 / 1.1e-50 / 649
Thioredoxin H-type / FK826529 / 2.4e-67 / 655
Cytochrome b561,complete / FK826541 / 8.1e-10 / 288
Cytochrome b5 domain-containing protein-like / FK826542 / 7.1e-29 / 386
Cytochrome B6-F complex iron-sulfur subunit / FK826543 / 3.4e-24 / 606
Cytochrome c oxidase, Vc subunit / FK826544 / 1.4e-16 / 358
Chlorophyll A/B binding protein / FK826554 / 2.0e-24 / 844
Thiazole biosynthetic enzyme / FK826557 / 1.7e-34 / 457
Ferredoxin / FK826558 / 9.4e-26 / 329
Light-inducible protein ATLS1, complete / FK826560 / 3.5e-17 / 604

Ribulose bisphosphate carboxylase small chain

/ FK826601 /

9.8e-38

/

362

Solanesyl diphosphate synthase 2

/ FK826605 /

1.9e-48

/

295

Glycolate oxidase 2

/ FK826616 /

3.7e-17

/

421

Solanesyl diphosphate synthase 2

/ FK826617 /

1.0e-37

/

684

10 kDa photosystem II polypeptide

/ GH985187 /

1.1e-60

/

452

ATP binding

/ GH985203 /

6.2e-05

/

443

Chlorophyll A/B binding protein precursor

/ GH985219 /

5.1e-55

/

606

Malate dehydrogenase

/ GH985225 /

8.2e-37

/

631

H-type thioredoxin

/ GH985227 /

2.8e-18

/

629

ATP synthase protein 9

/ GH985238 /

4.8e-53

/

345

ATP-dependent protease catalytic subunit

/ GH985258 /

1.1e-05

/

476

Polyphenol oxidase A1 chloroplast precursor / JK714298 / 2.8e-74 / 347
Photosystem I reaction center subunit II / JK714303 / 1.2e-29 / 327
Phytochrome A / JK714306 / 1.5e-14 / 378
Phytochrome A / JK714310 / 1.1e-47 / 376
Ribonucleoprotein A chloroplast precursor / JK714314 / 4.9e-22 / 804
Vacuolar ATP synthase 16 kDa proteolipid subunit / JK714318 / 1.0e-37 / 160
29 kDa ribonucleoprotein chloroplast precursor / JK714321 / 1.7e-34 / 909
Cytochrome P450 like / JK743772 / 1.4e-67 / 1005
Cytochrome P450-like TBP protein / JK743773 / 1.8e-71 / 513
Cytochrome P450 like / JK743776 / 8.1e-10 / 201
GDP dissociation inhibitor 1 / JK743784 / 7.7e-83 / 1043
NADP +isocitrate dehydrogenase / JK743798 / 2.9e-34 / 167
Chloroplast pigment-binding protein / JK743799 / 3.1e-30 / 635
Adenine nucleotide translocator / JK743809 / 3.1e-87 / 596
ATP-dependent subunit of the HslUV protease / JK743812 / 3.8e-31 / 574
Stress response
Stress response (contd.) / Drought-induced protein SDi-6 / FK826443 / 5.7e-22 / 377
Acyl-[acyl-carrier protein] desaturase / FK826449 / 1.1e-80 / 601
Purple acid phosphatase-like protein / FK826454 / 8.5e-43 / 562
Low temperature and salt responsive protein / FK826467 / 1.9e-12 / 411
Purple acid phosphatase-like protein / FK826471 / 4.5e-43 / 572
Wound-induced protein 1 / FK826507 / 3.4e-34 / 633
Wound-induced protein 1 / FK826508 / 1.8e-51 / 502
Ozone-responsive stress-related protein-like / FK826510 / 2.4e-67 / 593
Low temperature and salt responsive protein / FK826526 / 1.9e-55 / 411
Acyl carrier protein / FK826548 / 5.2e-38 / 272

Peptidyl-prolyl cis-trans isomerase

/ FK826604 / 2.4e-67 / 515

Disease resistance response protein-like

/ GH985151 /

1.2e-29

/

268

Temperature-induced lipocalin (TIL)

/ GH985161 /

9.3e-30

/

591

Dehydration-induced protein RD22-like protein 2

/ GH985168 /

2.2e-28

/

618

Dehydrin

/ GH985207 /

9.4e-26

/

398

T-complex protein 1, alpha subunit

/ GH985239 /

1.1e-22

/

490

Heme oxygenase 2 precursor

/ GH985267 /

1.8e-31

/

642

Al-induced protein / JK714300 / 3.5e-17 / 422
Al-induced protein / JK714301 / 5.4e-16 / 422
Acyl-CoA-binding protein / JK714337 / 7.8e-15 / 181
Acyl-CoA-binding protein / JK714338 / 8.3e-24 / 150
Ultraviolet-B-repressible protein / JK743778 / 7.6e-12 / 232
Al-induced protein / JK743788 / 1.1e-47 / 422
Transporter / Drm3 / FK826407 / 3.8e-23 / 245
Aquaporin / FK826409 / 4.6e-18 / 332
Transporter / FK826482 / 1.9e-44 / 641
Transporter / FK826499 / 1.6e-47 / 631
Importin like protein / FK826513 / 7.6e-47 / 626
36kDA porin II / FK826545 / 3.8e-18 / 229
Lipid transfer protein isoform 1 / FK826555 / 2.6e-14 / 651
Lipid transfer protein precursor / FK826556 / 3.8e-13 / 573

Tonoplast intrinsic protein 1

/ GH98515 / 1.1e-31 / 525

Putative ABC transporter

/ GH985167 / 7.7e-21 / 545

MipC

/ GH985184 / 2.7e-59 / 617

Tonoplast intrinsic protein bobTIP26-1

/ GH985188 / 1.7e-77 / 497

Lipid transfer protein 4

/ GH985223 / 1.9e-22 / 636

Lipid transfer protein precursor

/ GH985252 / 4.8e-18 / 332

Ankyrin

/ GH985259 / 4.6e-23 / 633

Plasma membrane associated protein

/ GH985268 /

2.0e-21

/

652

Ras-related GTP-binding protein / JK714329 / 1.7e-67 / 302
MipB gene / JK743766 / 9.8e-18 / 304
Hormone biosynthesis and signalling / Auxin-repressed protein-like protein / FK826404 / 9.5e-34 / 623
Aux/IAA protein / FK826518 / 7.6e-12 / 389
Leucine-rich repeat protein / FK826528 / 5.2e-38 / 318
Auxin-repressed protein / FK826531 / 6.8e-51 / 627

DELLA protein GAI1 (Gibberellic acid-insensitive mutant protein 1)