Online source 5.Orthologs between SlOFPs and AtOFPs,Paralogsand tandem duplicationof SlOFPs
Part 1. Alignment of amino acids in OVATE domain of SlOFPs and AtOFPs
Part 2.The putative paralog pairs of SlOFPs
Part 3.The putative orthologs between SlOFPs and AtOFPs
Part 4. The predicted genes in tomato SL2.40ch10:62260001..62315000 containing SlOFP21-SlOFP28
Part 5. A dot plot of two DNA sequences, tomato SL2.40ch10:62260001..62315000 containing SlOFP21-SlOFP28, and potato chr10: 48800001..48850000
Part 1. Alignment of amino acids in OVATE domain of SlOFPs and AtOFPs
AtOFP15 SDDPYSDFKRSMEEMVEAH------ALHHDWKS-LEKLLLQFLKVN--AKTSHRYIFAAFVDLLMNLALD
AtOFP18 SNDPYTDFKNSMEKMVEVH------VLHHDWIS-LEKLLFWFLKVN--VKASHRYIFAAFVDLVLNLAV-
SlOFP3 SNDPFVDFRKSMEEMVEAY------EIK-DWEN-LEELLTCYLKVN--CKSNHGYIVGAFVDLLVNLAT-
SlOFP29 SEDPYLDFKKSMEEMVDTH------EIKDWES-LQELLQWYLKMN--GKNNHGFIIGAFVDLLIGFTPS
AtOFP13 SEDPYGDFRRSMEEMVTSHG------ELAKDWES-LESMLAWYLRMN--GRKSHGVIVSAFVDLLSGLSDS
SlOFP10 SKDPYLDFKKSMKEMVESQG------IKDWDN-LQELLACYLKLN--GEVNHGFVLGAFVDLLVELVIP
SlOFP23 SRDPYEDIKYFLEKMVDE------NLEIEDWEESLEELCGWLLEIN--EKNIHKYIVGAFCDLWMSYSC-
SlOFP27 SRDPYEDIKYFLERMVDE------NLEIEDWKESLEELCGWLLEIN--EKNIHKYIVGAFCDLWMSYSC-
SlOFP24 HGDPYEDIKYFLERMVDERMV--DENLEIEDWEKSLEELCGWLLEIN--DKNIHKFIIGAFCDLWMSYSG-
SlOFP25 SIDPYGCIKKHMEIMVEE------NQGIKDWKESLKEICALYLEINYNDKNIHKFIIGAFCDLWMSYSG-
SlOFP26 SIDPYGYIKKYMEITVEE------NQGIKDWKESLKEICAWYLENNDNDKNIHKFIIGAFCDLWMSYSG-
SlOFP28 SIDPYGYIKKHMEMMVEE------NQGIKDWKESLKEICALYLEINYIDKNIHRFIIGAFCDLWMSYSG-
SlOFP22 SMDPYGSFKKSMVKMVEA------NLGIKDWNEFLEEMLAWYLEVN--EKNNHKYIIGAFCDLWISYSFT
SlOFP11 SMDPLTDIKESIKEMVKN------VGIKDWKE-MEELVYCYIVLN--SEEVHCFIKEAFLSLC------
SlOFP7 SPSPYDDFRQSMQEMMEARL-----KDQGKINWEFMEELLFCYLDLN--DKKSYKYILSAFVDQIVILREN
ATOFP14 TDEPQEDFRQSMVEMMESKLG----MRESEVDWDLMEELLFCYLDLN--DKKSHKFILSAFVDLIIALREK
SlOFP18 SPDPYLDFRRSMQEMVEAR----GLS-DIRGNWESLHELLMCYFTLN--PKSTHKYIVGAFSDLIVCLMES
SlOFP19 SPDPYSDFRRSMQEMVEAH----ELT-NVKANWGFLHELLLCYLNLN--PKHTHKYIIRAYSDLVVSLMSM
AtOFP11 SPDPYRDFGRSMREMVEAR----DLTRDVVADREYLHELLFCYLYLN--PKHTHRFIVSAFADTLLWLLSP
ATOFP12 SPDPYNDFRRSMQEMIDAVTNAGDLRR-----YEFLHELLLSYLSLN--AADTHKFIIRAFADILVSLLSD
AtOFP16 SPDPLTDFRRSMQEMIDAAIDAGELSRDPNDGYDFLDELLLTYLSLN--PADTHKFVIRAFSDILVSLLSE
OVATE SQDPYEDFKRSMMEMILEKE------MFEKN-ELEQLLQCFLSLN--GKHYHGVIVEAFSDIWETLFLG
SlOFP6 SEDPYEDFKSSMMEMILEKK------IFEKN-DLEQLLQCFLSLN--AKNCHGVIVEAFSEIWETLFSP
ATOFP7 SEDPYEDFKGSMMEMIVEKK------MFEVA-ELEQLLSCFLSLN--AKRHHRAIVRAFSEIWVALFSG
SlOFP9 SVDPYDDFKNSMKEMIMEKE------MFEAE-DLEQLLLCFLSLN--SRHHHAIIVEAFTEIWEELFGK
SlOFP16 TNDPHNDFRTSMLEMIVEKQ------IFGLK-DLQRLLHCFLSLN--SPSFHKIIFEVFAEIFETLFH-
AtOFP8 SKDPYEDFRTSMVEMIVERQ------IFAPA-ELQQLLQCFLSLN--SRQHHKVIVQVFLEIYATLFSP
SlOFP31 SKDPYGDFRNSMLEMIMENQ------IFGAK-ELENLLKCFLKLN--SQYHHEVIIDVFTEICEALFSS
SlOFP2 SDDPYLDFRQSMLQMILEKE------IYSKD-DLKELLNCFLQLN--SPYYHGIIVRAFTEIWNGVFSL
SlOFP21 SDDPYVDFRQSMLQMILEKE------IYSKD-ELRELLNCFLQLN--SPYYHGIIVRAFTEIWHCVFSV
AtOFP6 SDDPYLDFRQSMLQMILENQ------IYSKD-ELRELLQCFLSLN--SHYHHGIIVRAFSEIWEDVSSA
ATOFP19 SDDPYLDFRQSMLQMILENE------IYSKN-DLRELLHCFLSLN--EPYHHGIIIRAFSEIWDGVFSA
SlOFP4 SDDPFYDFKKSMLHMIIEKE------IYSFE-DLEDLLNCFLKLN--SPSHHNIIIQAFTEILNDNKK-
SlOFP30 SDDPFGDFRRSMLQMIMEKE------IYSYD-DLNELLNCFLQLN--SPSHHDIILQAFMEIWNNGKNY
SlOFP5 SFDPFSDFRDSMIEMITQRG------IKSSE-ELEELLACYLTLN--CDEYHDIIIKVFRQVWFELNQI
AtOFP5 SSDPQKDFRDSMIEMIMENG------INHPE-ELKELLVCYLRLN--TDEYHDMIISVFQQVHNDFNFH
SlOFP14 SIDPEKDFRESMVEMVVENN------IRASK-ELENLLACYLSLN--SNEYHDLIIKAFEQIWFDLSDL
ATOFP3 SVDPEKDFRESMVEMIMENK------MREQK-DLEDLLACYLSLN--SSEYHDVIIKAFENTWLHLTQG
AtOFP2 SVDPKKDFRESMIEMIEENN------IRASK-DLEDLLACYLTLN--PKEYHDLIIHVFEQIWLQLTKT
ATOFP4 SIDPSKDFRESMVEMIAENN------IRTSN-DMEDLLVCYLTLN--PKEYHDLIIKVFVQVWLEVINS
SlOFP17 SKNPQKDFKESMVEMIVKNN------IKTSK-DLEELLACYLLLN--SHHYHHLIITVFKQIWFDLQLK
SlOFP20 SRNPQKDFRESMVEMIIENN------ITTSK-DLEELLACYLSLN--SDEYHDIIIKVFKQIWFEITEI
AtOFP1 SVDPKRDFKESMEEMIAENK------IRATK-DLEELLACYLCLN--SDEYHAIIINVFKQIWLDLNLP
SlOFP15 SYDPREDFRESIEQMIIANR------ICDPK-DLRRLLNYYVSMN--AEEYRGVILEVFHQVCTTFFLS
AtOFP9 SFDPRKDFRDSMVEMIVANK------IKEAD-ELRSLLEYYLSMN--PREYRSAILEIFYEVCADLFRS
AtOFP10 SINPFEDYKKSMNQMIEER------YIETESELKELLRCFLDIN--PSPQHNLIVRAFVDVCSHLQPP
Pp1s283_17V6.1 SSDPYNDFHLSMVSMIEEEG------LQECEAELEELFQYYLDLN--PKGHHEVLHKVIGDIRTDYLLK
SlOFP12 TENPKMDFLHSMEQMTKVYG---AQRGDMVDWE-FMEELLTWFLKIN--NMKNQHFIVAAFIDLCLGGHVQ
SlOFP13 -EDACRSFENYLAEMIVEEG------KMRDIMDVEELLYCWKNLK--SPVFIDLVCRFYGELCKDLFSH
AtOFP17 ----CRSFENYLIHLIVEEG------KIDDLMDIEELLFCWKNLK--SPVFIELVSRFYGELCRDLFSG
ATOFP20 ----CRRFENYMIHMFVEDG------KIDDLMDMEELLYYWKNLN--SPIFIDLVTRFYGELCTDLFP-
SlOFP8 --KGSLTLLKKMEELEMVEG------EDMDHVLDIEEVLQCYTLLN--SPVYVDIVDRFFMDMYTEFSIR
Part 2. The putative paralog pairs of SlOFPs
One paralog / The other paralog / Smith-Waterman score / Z-score / Bits / E Value / Identity / Similar / Overlap (aa)Name / Length (aa) / Name / Length (aa)
OVATE / 352 / SlOFP6 / 391 / 687 / 896.4 / 174.5 / 4E-44 / 43.7% / 66.7% / 403
SlOFP2 / 182 / SlOFP21 / 178 / 631 / 785.6 / 151.9 / 6E-38 / 71.1% / 87.8% / 180
SlOFP10 / 229 / SlOFP29 / 244 / 523 / 688.2 / 134.7 / 1.6E-32 / 50% / 67.2% / 244
SlOFP17 / 251 / SlOFP20 / 321 / 568 / 734.5 / 143.8 / 4.2E-35 / 46% / 62.2% / 328
SlOFP18 / 225 / SlOFP19 / 211 / 401 / 493.2 / 98.4 / 1.2E-21 / 41.9% / 64% / 236
SlOFP26 / 269 / SlOFP28 / 233 / 1069 / 1205.2 / 230.5 / 2.5E-61 / 81.5% / 88.6% / 254
Part 3. The putative orthologs between SlOFPs and AtOFPs
SlOFPs / AtOFPs / Smith-Waterman score / Z-score / Bits / E Value / Identity / Similar / Overlap (aa)Name / Length (aa) / Name / Length (aa)
OVATE / 352 / AtOFP7 / 315 / 436 / 501.6 / 101.2 / 3.9E-22 / 37.4% / 56.9% / 369
SlOFP2 / 182 / AtOFP19 / 183 / 413 / 582.2 / 114.3 / 1.3E-26 / 51.1% / 76.6% / 184
SlOFP3 / 298 / AtOFP15 / 261 / 404 / 475 / 95.7 / 1.2E-20 / 39.7% / 62.5% / 312
SlOFP5 / 377 / AtOFP5 / 349 / 439 / 516.9 / 104.2 / 5.6E-23 / 33.7% / 61.3% / 395
SlOFP6 / 391 / AtOFP7 / 315 / 404 / 443.7 / 90.6 / 6.6E-19 / 34.1% / 55.7% / 393
SlOFP7 / 275 / AtOFP14 / 294 / 409 / 551.5 / 109.9 / 6.5E-25 / 38.4% / 62.9% / 294
SlOFP13 / 188 / AtOFP17 / 195 / 358 / 451.2 / 90.2 / 2.5E-19 / 44.1% / 70.7% / 188
SlOFP14 / 351 / AtOFP2 / 320 / 516 / 552.3 / 110.6 / 5.9E-25 / 40.1% / 65.1% / 364
SlOFP15 / 168 / AtOFP9 / 411 / 340 / 428.8 / 87 / 4.40E-18 / 54% / 82.5% / 126
SlOFP18 / 225 / AtOFP12 / 226 / 321 / 382.8 / 78 / 1.60E-15 / 37.2% / 65.5% / 226
SlOFP19 / 211 / AtOFP12 / 226 / 387 / 507.5 / 101 / 1.8E-22 / 42.4% / 66.1% / 224
SlOFP29 / 244 / AtOFP13 / 260 / 474 / 592.7 / 117.2 / 3.3E-27 / 45% / 66.9% / 269
SlOFP31 / 274 / AtOFP8 / 221 / 345 / 413.6 / 84 / 3.20E-17 / 34.5% / 56.9% / 281
Part 4. The predicted genes in tomato SL2.40ch10:62260001..62315000 containing SlOFP21-SlOFP28
No. / Gene Locus / Gene transcript region / Gene functional description1 / Solyc10g082030.1.1 / SL2.40ch10:62258959..62262932- / Peroxiredoxin
2 / Solyc10g082040.1.1 / SL2.40ch10:62264261..62265744- / Receptor expression-enhancing protein 3
3 / Solyc10g082050.1.1 / SL2.40ch10:62268995..62269531+ / Plant-specific domain TIGR01568 family protein, contains Interpro domain(s) IPR006458, SlOFP21
4 / Solyc10g082060.1.1 / SL2.40ch10:62274760..62275518- / Plant-specific domain TIGR01568 family protein, contains Interpro domain(s) IPR006458, SlOFP22
5 / Solyc10g083060.1.1 / SL2.40ch10:62281038..62281347+ / Nuclear transport factor 2 family protein
6 / Solyc10g083070.1.1 / SL2.40ch10:62291057..62292046- / Unknown Protein (AHRD V1); contains Interpro domain(s) IPR006458, SlOFP23
7 / SL2.40ch10: 62294354..62293690- / SlOFP24
8 / SL2.40ch10: 62301706..62302128- / SlOFP25
9 / Solyc10g083080.1.1 / SL2.40ch10:62296279..62297088- / Unknown Protein (AHRD V1); contains Interpro domain(s) IPR006458, SlOFP26
10 / Solyc10g083090.1.1 / SL2.40ch10:62299100..62300089- / Plant-specific domain TIGR01568 family protein, contains Interpro domain(s) IPR006458, SlOFP27
11 / Solyc10g083100.1.1 / SL2.40ch10:62304379..62305080- / Unknown Protein (AHRD V1); contains Interpro domain(s) IPR006458, SlOFP28
12 / Solyc10g083110.1.1 / SL2.40ch10:62306678..62308436- / Dihydrodipicolinatesynthase 2
13 / Solyc10g083120.1.1 / SL2.40ch10:62310434..62314944- / Ubiquitin-conjugating enzyme family protein-like
Part 5. A dot plot of two DNA sequences, tomato SL2.40ch10:62260001..62315000containing SlOFP21-SlOFP28, and potato chr10: 48800001..48850000. Tomato and potato have similar kind of tandem duplication in this homologous region.Dot plot made by Advanced PipMaker(Schwartz et al. 2000).
References
Schwartz S, Zhang Z, Frazer KA, Smit A, Riemer C, Bouck J, Gibbs R, Hardison R, Miller W (2000) PipMaker--a web server for aligning two genomic DNA sequences. Genome Res 10 (4):577-586