Table S1. Summary statistics of genetic variation at eight presumably neutral microsatellite loci in the studied brown trout populations. Ho= Observed heterozygosity, He= Expected heterozygosity, a = number of alleles observed, as = allele size range (basepairs), n = sample size (individuals), pa = number of private alleles, ar = allelic richness.
Population / LocusStr60 / Str73 / Ssosl417 / Str15 / Ssa197 / Ssosl438 / Ssa85 / T3-13 / Mean / Std. dev.
ASBK-92 / Ho / 0.288 / 0.610 / 0.831 / 0.475 / 0.864 / 0.729 / 0.542 / 0.966 / 0.663 / 0.226
He / 0.340 / 0.653 / 0.817 / 0.562 / 0.830 / 0.682 / 0.496 / 0.900 / 0.660 / 0.189
a / 3 / 5 / 7 / 4 / 8 / 5 / 5 / 14 / 6.375 / 3.462
as / 95-105 hggh / 141-151 / 173-189 / 220-226 / 130-162 / 103-113 / 106-116 / 177-223
n / 59 / 59 / 59 / 59 / 59 / 59 / 59 / 59
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.373 / 3.746 / 6.924 / 3.967 / 7.109 / 4.366 / 4.312 / 11.237
FISK-92 / Ho / 0.344 / 0.688 / 0.875 / 0.406 / 0.875 / 0.656 / 0.406 / 0.875 / 0.641 / 0.228
He / 0.365 / 0.634 / 0.845 / 0.536 / 0.842 / 0.661 / 0.471 / 0.886 / 0.655 / 0.192
a / 2 / 3 / 7 / 4 / 9 / 4 / 4 / 13 / 5.750 / 3.694
as / 95-99 / 141-147 / 173-189 / 220-226 / 130-162 / 103-111 / 110-116 / 177-217
n / 32 / 32 / 32 / 32 / 32 / 32 / 32 / 32
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.000 / 3.000 / 6.972 / 3.998 / 8.365 / 3.992 / 3.904 / 11.816
KRAG-92 / Ho / 0.484 / 0.645 / 0.935 / 0.613 / 0.742 / 0.419 / 0.516 / 0.903 / 0.657 / 0.190
He / 0.373 / 0.696 / 0.863 / 0.544 / 0.821 / 0.652 / 0.492 / 0.906 / 0.668 / 0.190
a / 2 / 4 / 7 / 4 / 8 / 4 / 5 / 15 / 6.125 / 4.051
as / 95-99 / 141-151 / 173-189 / 220-226 / 130-162 / 103-113 / 106-116 / 177-221
n / 31 / 31 / 31 / 31 / 31 / 31 / 31 / 31
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.000 / 3.919 / 7.000 / 3.994 / 7.606 / 3.710 / 4.629 / 13.547
ODR1-92 / Ho / 0.433 / 0.767 / 0.867 / 0.500 / 0.867 / 0.633 / 0.633 / 0.833 / 0.692 / 0.168
He / 0.413 / 0.635 / 0.853 / 0.581 / 0.816 / 0.677 / 0.631 / 0.885 / 0.686 / 0.158
a / 2 / 3 / 9 / 4 / 10 / 4 / 5 / 14 / 6.375 / 4.173
as / 95-99 / 141-147 / 171-191 / 220-226 / 130-170 / 103-113 / 106-116 / 177-221
n / 30 / 30 / 30 / 30 / 30 / 30 / 30 / 30
pa / 0 / 0 / 0 / 0 / 1 / 0 / 0 / 0
ar / 2.000 / 3.000 / 8.594 / 3.932 / 8.849 / 3.733 / 4.730 / 12.248
ODR2-92 / Ho / 0.200 / 0.543 / 0.743 / 0.714 / 0.800 / 0.543 / 0.457 / 0.943 / 0.618 / 0.232
He / 0.342 / 0.618 / 0.837 / 0.594 / 0.833 / 0.648 / 0.525 / 0.864 / 0.658 / 0.181
a / 2 / 4 / 8 / 4 / 8 / 4 / 5 / 12 / 5.875 / 3.227
as / 95-99 / 141-151 / 173-191 / 220-226 / 130-162 / 103-111 / 106-116 / 183-219
n / 35 / 35 / 35 / 35 / 35 / 35 / 35 / 35
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.000 / 3.865 / 7.609 / 4.000 / 7.607 / 3.952 / 4.863 / 10.412
Table S1 (continued)
Population / LocusStr60 / Str73 / Ssosl417 / Str15 / Ssa197 / Ssosl438 / Ssa85 / T3-13 / Mean / Std. dev.
ASSE-92 / Ho / 0.273 / 0.697 / 0.758 / 0.424 / 0.710 / 0.788 / 0.545 / 0.970 / 0.646 / 0.221
He / 0.396 / 0.659 / 0.848 / 0.521 / 0.768 / 0.722 / 0.566 / 0.853 / 0.667 / 0.163
a / 3 / 3 / 8 / 4 / 8 / 5 / 4 / 10 / 5.625 / 2.669
as / 95-101 / 141-147 / 171-189 / 220-226 / 130-162 / 130-113 / 110-116 / 177-215
n / 33 / 33 / 33 / 33 / 31 / 33 / 33 / 33
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.667 / 3.000 / 7.558 / 3.990 / 7.616 / 4.657 / 3.990 / 9.333
STAM-92 / Ho / 0.448 / 0.448 / 0.750 / 0.690 / 0.862 / 0.690 / 0.448 / 1,000 / 0.667 / 0.207
He / 0.390 / 0.629 / 0.841 / 0.677 / 0.832 / 0.691 / 0.488 / 0.887 / 0.679 / 0.175
a / 2 / 3 / 8 / 4 / 9 / 5 / 4 / 12 / 5.875 / 3.441
as / 95-99 / 141-147 / 171-189 / 220-226 / 130-162 / 103-113 / 110-116 / 177-221
n / 29 / 29 / 28 / 29 / 29 / 29 / 29 / 29
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.000 / 3.000 / 7.770 / 4.000 / 8.450 / 4.517 / 3.945 / 11.371
ASBK-04 / Ho / 0.300 / 0.620 / 0.860 / 0.400 / 0.800 / 0.735 / 0.480 / 0.820 / 0.627 / 0.212
He / 0.343 / 0.667 / 0.845 / 0.446 / 0.809 / 0.661 / 0.474 / 0.887 / 0.642 / 0.202
a / 3 / 3 / 8 / 4 / 9 / 4 / 5 / 14 / 6.250 / 3.845
as / 95-105 / 141-147 / 173-191 / 220-226 / 130-162 / 103-113 / 106-116 / 177-223
n / 50 / 50 / 50 / 50 / 50 / 49 / 50 / 50
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.689 / 3.000 / 7.405 / 3.980 / 8.524 / 3.699 / 4.657 / 12.134
FISK-04 / Ho / 0.278 / 0.537 / 0.852 / 0.648 / 0.796 / 0.759 / 0.463 / 0.759 / 0.637 / 0.197
He / 0.241 / 0.610 / 0.855 / 0.620 / 0.756 / 0.706 / 0.513 / 0.826 / 0.641 / 0.198
a / 2 / 4 / 8 / 4 / 9 / 6 / 5 / 12 / 6,250 / 3.240
as / 95-99 / 141-151 / 173-191 / 220-226 / 130-162 / 101-113 / 106-116 / 177-223
n / 54 / 54 / 54 / 54 / 54 / 54 / 54 / 54
pa / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0
ar / 2.000 / 3.407 / 7.626 / 3.998 / 7.970 / 4.940 / 4.393 / 9.683
KRAG-04 / Ho / 0.408 / 0.735 / 0.857 / 0.490 / 0.857 / 0.673 / 0.633 / 0,939 / 0.699 / 0.186
He / 0.435 / 0.562 / 0.847 / 0.598 / 0.781 / 0.706 / 0.572 / 0.891 / 0.674 / 0.158
a / 3 / 3 / 8 / 4 / 9 / 5 / 5 / 16 / 6.625 / 4.373
as / 95-105 / 141-147 / 173-191 / 220-226 / 130-162 / 103-113 / 106-116 / 177-223
n / 49 / 49 / 49 / 49 / 49 / 49 / 49 / 49
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.449 / 2.997 / 7.285 / 3.999 / 8.307 / 4.830 / 4.651 / 12.711
ODR1-04 / Ho / 0.480 / 0.660 / 0.720 / 0.480 / 0.820 / 0.735 / 0.520 / 0.980 / 0.674 / 0.177
He / 0.424 / 0.680 / 0.796 / 0.586 / 0.802 / 0.646 / 0.502 / 0.887 / 0.665 / 0.159
a / 2 / 5 / 7 / 4 / 8 / 5 / 5 / 16 / 6.500 / 4.243
as / 95-99 / 141-151 / 173-189 / 220-226 / 130-162 / 103-113 / 104-116 / 177-223
n / 50 / 50 / 50 / 50 / 50 / 49 / 50 / 50
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.000 / 4.378 / 6.797 / 3.995 / 7.846 / 3.898 / 4.268 / 12.389
Table S1 (continued)
Population / LocusStr60 / Str73 / Ssosl417 / Str15 / Ssa197 / Ssosl438 / Ssa85 / T3-13 / Mean / Std. dev.
ODR2-04 / Ho / 0.440 / 0.700 / 0.780 / 0.420 / 0.900 / 0.720 / 0.460 / 0.960 / 0.672 / 0.211
He / 0.368 / 0.631 / 0.836 / 0.459 / 0.836 / 0.671 / 0.526 / 0.889 / 0.652 / 0.192
a / 2 / 4 / 7 / 4 / 9 / 4 / 5 / 15 / 6.250 / 4.132
as / 95-99 / 141-151 / 173-189 / 220-226 / 130-162 / 103-111 / 106-116 / 177-223
n / 50 / 50 / 50 / 50 / 50 / 50 / 50 / 50
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.000 / 3.440 / 6.941 / 3.982 / 8.285 / 3.440 / 4.894 / 12.210
ASSE-04 / Ho / 0.653 / 0.714 / 0.796 / 0.612 / 0.878 / 0.796 / 0.347 / 1.000 / 0.724 / 0.196
He / 0.444 / 0.649 / 0.833 / 0.557 / 0.808 / 0.725 / 0.327 / 0.894 / 0.655 / 0.199
a / 2 / 4 / 8 / 4 / 9 / 6 / 5 / 14 / 6.500 / 3.780
as / 95-99 / 141-151 / 171-189 / 220-226 / 130-162 / 103-113 / 106-116 / 177-219
n / 49 / 49 / 49 / 49 / 49 / 49 / 49 / 49
pa / 0 / 0 / 0 / 0 / 0 / 1 / 0 / 0
ar / 2.000 / 3.699 / 7.282 / 3.912 / 8.136 / 5.315 / 4.562 / 12.183
STAM-04 / Ho / 0.160 / 0.540 / 0.880 / 0.500 / 0.720 / 0.740 / 0.520 / 0.920 / 0.623 / 0.246
He / 0.182 / 0.631 / 0.842 / 0.573 / 0.809 / 0.672 / 0.614 / 0.866 / 0.649 / 0.219
a / 2 / 3 / 7 / 4 / 9 / 5 / 4 / 13 / 5.875 / 3.643
as / 95-99 / 141-147 / 173-189 / 220-226 / 130-162 / 103-113 / 110-116 / 177-219
n / 50 / 50 / 50 / 50 / 50 / 50 / 50 / 50
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 1.998 / 3.000 / 6.979 / 3.999 / 8.078 / 4.129 / 3.992 / 10.936
KRO-89 / Ho / 0.205 / 0.482 / 0.711 / 0.518 / 0.732 / 0.639 / 0.771 / 0.819 / 0.610 / 0.202
He / 0.198 / 0.472 / 0.829 / 0.492 / 0.771 / 0.669 / 0.778 / 0.883 / 0.636 / 0.232
a / 5 / 3 / 7 / 5 / 7 / 4 / 5 / 15 / 6.375 / 3.739
as / 91-105 / 143-147 / 173-189 / 220-228 / 118-154 / 103-111 / 104-118 / 181-223
n / 83 / 83 / 83 / 83 / 82 / 83 / 83 / 83
pa / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0
ar / 2.787 / 2.265 / 6.456 / 4.416 / 6.675 / 3.997 / 4.994 / 11.799
KRO-02 / Ho / 0.349 / 0.405 / 0.837 / 0.233 / 0.698 / 0.674 / 0.721 / 1.000 / 0.615 / 0.262
He / 0.321 / 0.470 / 0.730 / 0.274 / 0.649 / 0.697 / 0.728 / 0.869 / 0.592 / 0.213
a / 2 / 2 / 8 / 4 / 7 / 4 / 7 / 14 / 6.000 / 3.964
as / 95-99 / 145-147 / 171-189 / 220-226 / 118-158 / 103-111 / 104-120 / 187-223
n / 43 / 37 / 43 / 43 / 43 / 43 / 43 / 43
pa / 0 / 0 / 0 / 0 / 0 / 0 / 2 / 0
ar / 2.000 / 2.000 / 7.170 / 3.824 / 5.895 / 3.989 / 6.010 / 11.503
KAP-89 / Ho / 0.545 / 0.773 / 0.591 / 0.682 / 0.864 / 0.455 / 0.864 / 0.955 / 0.716 / 0.176
He / 0.458 / 0.655 / 0.811 / 0.654 / 0.831 / 0.557 / 0.744 / 0.912 / 0.703 / 0.151
a / 3 / 4 / 7 / 5 / 8 / 4 / 6 / 16 / 6.625 / 4.138
as / 95-105 / 141-147 / 173-193 / 220-228 / 118-162 / 103-111 / 104-118 / 187-223
n / 22 / 22 / 22 / 22 / 22 / 22 / 22 / 22
pa / 0 / 0 / 1 / 0 / 0 / 0 / 0 / 0
ar / 3.000 / 4.000 / 7.000 / 5.000 / 8.000 / 4.000 / 6.000 / 16.000
Table S1 (continued)
Population / LocusStr60 / Str73 / Ssosl417 / Str15 / Ssa197 / Ssosl438 / Ssa85 / T3-13 / Mean / Std. dev.
KAP-02 / Ho / 0.390 / 0.561 / 0.805 / 0.421 / 0.707 / 0.585 / 0.829 / 1.000 / 0.662 / 0.211
He / 0.432 / 0.600 / 0.799 / 0.565 / 0.804 / 0.615 / 0.744 / 0.906 / 0.683 / 0.156
a / 3 / 4 / 8 / 5 / 9 / 5 / 5 / 14 / 6.625 / 3.583
as / 95-105 / 141-147 / 171-189 / 220-228 / 118-162 / 103-113 / 104-114 / 183-221
n / 41 / 41 / 41 / 38 / 41 / 41 / 41 / 41
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.788 / 3.535 / 6.819 / 4.578 / 7.979 / 4.515 / 4.954 / 12.545
TN-94 / Ho / 0.429 / 0.510 / 0.898 / 0.673 / 0.878 / 0.531 / 0.796 / 0.857 / 0.696 / 0.187
He / 0.491 / 0.572 / 0.847 / 0.713 / 0.828 / 0.584 / 0.736 / 0.904 / 0.709 / 0.148
a / 4 / 4 / 10 / 4 / 7 / 5 / 6 / 20 / 7.500 / 5.451
as / 91-105 / 141-147 / 161-191 / 220-226 / 130-158 / 103-113 / 104-116 / 177-223
n / 49 / 49 / 49 / 49 / 49 / 49 / 49 / 49
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 3.148 / 3.442 / 8.772 / 4.000 / 6.980 / 4.535 / 5.361 / 14.776
TO-94 / Ho / 0.277 / 0.255 / 0.766 / 0.426 / 0.957 / 0.696 / 0.787 / 0.936 / 0.637 / 0.281
He / 0.299 / 0.350 / 0.791 / 0.417 / 0.818 / 0.649 / 0.791 / 0.859 / 0.622 / 0.231
a / 2 / 3 / 9 / 3 / 8 / 4 / 6 / 12 / 5.875 / 3.523
as / 95-99 / 141-147 / 173-195 / 222-226 / 118-166 / 103-111 / 106-118 / 181-221
n / 47 / 47 / 47 / 47 / 46 / 46 / 47 / 47
pa / 0 / 0 / 2 / 0 / 0 / 0 / 0 / 0
ar / 2.000 / 2.854 / 7.354 / 2.999 / 7.297 / 3.731 / 5.920 / 10.309
BR-95 / Ho / 0.458 / 0.559 / 0.780 / 0.695 / 0.932 / 0.542 / 0.729 / 0.932 / 0.703 / 0.177
He / 0.498 / 0.632 / 0.846 / 0.599 / 0.861 / 0.548 / 0.738 / 0.871 / 0.699 / 0.150
a / 3 / 3 / 10 / 4 / 11 / 5 / 6 / 18 / 7.500 / 5.210
as / 95-105 / 141-147 / 161-191 / 220-226 / 118-166 / 103-113 / 104-116 / 177-221
n / 59 / 59 / 59 / 59 / 59 / 59 / 59 / 59
pa / 0 / 0 / 0 / 0 / 0 / 0 / 0 / 0
ar / 2.996 / 3.000 / 7.992 / 3.373 / 10.538 / 3.969 / 5.514 / 13.633
Total / Ho / 0.373 / 0.593 / 0.804 / 0.525 / 0.822 / 0.654 / 0.594 / 0.922
He / 0.369 / 0.605 / 0.829 / 0.551 / 0.805 / 0.659 / 0.593 / 0.882
a / 2.571 / 3.524 / 7.905 / 4.095 / 8.524 / 4.619 / 5.095 / 14.238
1
ASBK92 / FISK92 / KRAG92 / ODR1-92 / ODR2-92 / ASSE92 / STAM92 / ASBK04 / FISK04 / KRAG04 / ODR1-04 / ODR2-04 / ASSE04 / STAM04 / KRO89 / KRO02 / KAP89 / KAP02 / TN94 / TO94 / BR95ASBK92 / - / 0.002
(0.00-0.01) / 0.000
(0.00-0.01) / 0.012
(0.00-0.03) / 0.002
(0.00-0.01) / 0.012
(0.00-0.03) / 0.003
(0.00-0.01) / 0.009
(0.00-0.02) / 0.016
(0.01-0.02) / 0.014
(0.01-0.02) / 0.016
(0.00-0.03) / 0.006
(0.00-0.01) / 0.011
(0.00-0.02) / 0.010
(0.00-0.02) / 0.056
(0.03-0.09) / 0.066
(0.04-0.10) / 0.066
(0.02-0.13) / 0.051
(0.01-0.11) / 0.043
(0.02-0.07) / 0.074
(0.03-0.14) / 0.033
(0.01-0.07)
FISK92 / ns / - / 0.000
(0.00-0.01) / 0.007
(0.00-0.03) / 0.001
(0.00-0.01) / 0.011
(0.00-0.03) / 0.005
(0.00-0.02) / 0.000
(0.00-0.00) / 0.014
(0.00-0.03) / 0.007
(0.00-0.02) / 0.013
(0.00-0.03) / 0.005
(0.00-0.02) / 0.011
(0.00-0.02) / 0.013
(0.00-0.03) / 0.060
(0.02-0.10) / 0.067
(0.03-0.11) / 0.060
(0.02-0.13) / 0.049
(0.00-0.11) / 0.035
(0.01-0.07) / 0.066
(0.02-0.14) / 0.033
(0.01-0.07)
KRAG92 / ns / ns / - / 0.001
(0.00-0.01) / 0.000
(0.00-0.01) / 0.004
(0.00-0.02) / 0.002
(0.00-0.01) / 0.000
(0.00-0.01) / 0.017
(0.00-0.03) / 0.009
(0.00-0.03) / 0.009
(0.00-0.02) / 0.003
(0.00-0.02) / 0.006
(0.00-0.01) / 0.013
(0.00-0.03) / 0.062
(0.02-0.10) / 0.066
(0.03-0.10) / 0.043
(0.01-0.10) / 0.041
(0.01-0.09) / 0.035
(0.01-0.06) / 0.072
(0.01-0.16) / 0.026
(0.01-0.06)
ODR1-92 / *** / * / ns / - / 0.003
(0.00-0.01) / 0.008
(0.00-0.03) / 0.006
(0.00-0.02) / 0.010
(0.00-0.02) / 0.017
(0.00-0.04) / 0.009
(0.00-0.02) / 0.017
(0.00-0.04) / 0.015
(0.00-0.03) / 0.014
(0.00-0.05) / 0.016
(0.00-0.04) / 0.059
(0.03-0.08) / 0.064
(0.04-0.09) / 0.035
(0.00-0.07) / 0.038
(0.01-0.06) / 0.028
(0.01-0.05) / 0.070
(0.02-0.17) / 0.031
(0.01-0.06)
ODR2-92 / ns / ns / ns / ns / - / 0.010
(0.00-0.03) / 0.001
(0.00-0.01) / 0.009
(0.00-0.02) / 0.023
(0.00-0.04) / 0.003
(0.00-0.01) / 0.023
(0.00-0.06) / 0.009
(0.00-0.03) / 0.010
(0.00-0.02) / 0.015
(0.00-0.03) / 0.048
(0.01-0.09) / 0.055
(0.02-0.09) / 0.054
(0.01-0.12) / 0.044
(0.00-0.10) / 0.037
(0.02-0.05) / 0.079
(0.02-0.17) / 0.027
(0.01-0.05)
ASSE92 / ** / *** / ns / ** / * / - / 0.006
(0.00-0.02) / 0.008
(0.00-0.03) / 0.010
(0.00-0.04) / 0.004
(0.00-0.02) / 0.013
(0.00-0.02) / 0.002
(0.00-0.01) / 0.006
(0.00-0.02) / 0.003
(0.00-0.02) / 0.061
(0.02-0.09) / 0.077
(0.04-0.12) / 0.055
(0.02-0.09) / 0.052
(0.01-0.08) / 0.046
(0.02-0.07) / 0.066
(0.02-0.12) / 0.034
(0.01-0.06)
STAM92 / ns / ns / ns / * / ns / ns / - / 0.012
(0.00-0.03) / 0.016
(0.00-0.03) / 0.004
(0.00-0.01) / 0.015
(0.00-0.04) / 0.008
(0.00-0.03) / 0.002
(0.00-0.01) / 0.009
(0.00-0.03) / 0.055
(0.04-0.09) / 0.072
(0.04-0.10) / 0.058
(0.00-0.12) / 0.054