Supplementary Table 1 Genes regulated in response to both pre-anthesis heat priming and post-anthesis high temperature stress
ProbeSet ID / Fold changes of treatments / Gene Description
7P/7C / 9P/9C / PH/CH
Up regulated during heat priming and heat stress
Ta.11144.1 / 2.31 / - / 2.33 / Phospholipase D alpha 1
Ta.1207.1 / 20.66 / 0.18 / 2 / 12-oxophytodienoate reductase 2
Ta.26983.1 / 4.95 / 5.17 / 2.85 / Basic chitinase
Ta.261.1 / 2.3 / 5.23 / 3.12 / Heat shock protein 101
Ta.9140.1 / 2.86 / 4.2 / 2.95 / Heat shock protein 90.1
Ta.639.1 / - / 4.27 / 2.15 / FKBP-type peptidyl-prolyl cis-trans isomerase family protein
Ta.6634.1 / 2.93 / 2.06 / 2.14 / DNA gyrase A
Ta.25492.1 / 3.11 / - / 2.38 / NAD(P)-linked oxidoreductase superfamily protein
Ta.107485.1 / 3.55 / - / 2.2 / Nucleotide-diphospho-sugar transferases superfamily protein
Ta.59384.2 / 9.92 / 0.22 / 2.36 / RNI-like superfamily protein
Down regulated during heat priming and heat stress
Ta.21438.1 / 0.17 / 6.64 / 0.31 / Cytochrome P450, family 71, subfamily B
Ta.116369.1 / 0.49 / 3.23 / 0.31 / Cytochrome P450, family 71, subfamily B
Ta.121266.1 / 0.3 / - / 0.43 / Laccase 8
Ta.11685.1 / - / 0.48 / 0.48 / Nudix hydrolase homolog 2
Ta.81638.2 / 12.06 / 0.22 / 0.49 / Senescence-associated gene 12
Ta.79139.1 / 3.34 / 0.49 / 0.03 / Isocitrate lyase
Ta.79218.1 / 2.54 / 0.48 / 0.05 / Isocitrate lyase
Ta.2631.1 / 0.48 / 2.86 / 0.49 / Myo-inositol oxygenase 5
Ta.23909.1 / 0.39 / 0.37 / 0.23 / Beta glucosidase 43
Ta.23909.2 / - / 0.41 / 0.18 / Beta glucosidase 43
Ta.1229.1 / 0.24 / 4.73 / 0.34 / Threonine aldolase 1
Ta.3828.3 / 0.49 / - / 0.35 / Glycosyl hydrolase superfamily protein
Ta.1020.1 / 0.04 / 0.16 / 0.43 / NmrA-like negative transcriptional regulator family protein
Ta.5710.1 / 0.43 / - / 0.49 / Multidrug resistance-associated protein 5
Ta.6041.1 / 0.44 / - / 0.44 / Mechanosensitive ion channel family protein
Ta.9830.1 / 0.32 / 2.51 / 0.41 / Arabidopsis thaliana peroxygenase 2
Ta.11623.2 / 3.27 / 0.38 / 0.41 / BTB/POZ domain-containing protein
Ta.14491.1 / 0.47 / - / 0.11 / Haloacid dehalogenase-like hydrolase (HAD) superfamily protein
Ta.14700.1 / 0.39 / 3.95 / 0.44 / Major facilitator superfamily protein
Ta.4508.3 / 0.24 / - / 0.48 / Major facilitator superfamily protein
Ta.3005.1 / - / 0.45 / 0.48 / Porphyromonas-type peptidyl-arginine deiminase family protein

Note: 7P/7C: the fold change in primed plants (7P) vs the non-primed plants (7C) at the first priming event; 9P/9C: the fold change in primed plants (9P) vs the non-primed plants (9C) under the second priming event; PH/CH: the fold change in primed plants (PH) vs the non-primed plants (CH) in response to the post-anthesis high temperature stress. “-” means no significant folder change.

Legends of Supplementary figures

Supplementary Fig. 1 Venn diagrams of the global changes in gene expression profile in response to the pre-anthesis heat priming and post-anthesis high temperature stress in relation to the corresponding control

Note: 7P/7C: the fold change in primed plants (7P) vs the non-primed plants (7C) at the first priming event; 9P/9C: the fold change in primed plants (9P) vs the non-primed plants (9C) at the second priming event; PH/CH: the fold change in primed plants (PH) vs the non-primed plants (CH) due to the post-anthesis high temperature stress

Supplementary Fig. 2 Quantitative variations (expressed as average normalized volume) of spots which were differently expressed in response to the pre-anthesis heat priming and post-anthesis high temperature stress

Note: 7P/7C: the fold change in primed plants (7P) vs the non-primed plants (7C) at the first priming event; 9P/9C: the fold change in primed plants (9P) vs the non-primed plants (9C) at the second priming event; PH/CH: the fold change in primed plants (PH) vs the non-primed plants (CH) due to the post-anthesis high temperature stress

Supplementary Fig. 1

Supplementary Fig. 2