Journal Name: World Journal of Microbiology and Biotechnology

Article title: Loop-mediated isothermal amplification for rapid and convenient detection ofMycoplasma hyopneumoniae

Journal name: World Journal of Microbiology and Biotechnology

Author names: Jiahe Li·F. Chris Minion·Andrew C. Petersen·Fei Jiang·Sheng Yang·Panpan Guo·Jinxiang Li·Wenxue Wu

J. Li · F. Jiang · S. Yang · P. Guo · W. Wu (Corresponding author) Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, People’s Republic of China. E-mail address:

F. C. Minion · A. C. Petersen Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa

Jinxiang Li Chinese Academy of Agricultural Sciences

Online Resource 1Non-mycoplasmal bacteria tested in LAMP assay

Organism / strain / source / Result
Acinetobacter calcoaceticus / R. Griffith, ISU / －
Acinetobacter calcoaceticus / R. Griffith, ISU / －
Actinobacillus equuli / R. Griffith, ISU / －
Actinobacillus lignieresii / R. Griffith, ISU / －
Actinobacillus pleuropneumoniae / L20 Sero:5B / R.F. Ross, ISU / －
Actinobacillus pleuropneumoniae / R.F. Ross, ISU / －
Actinobacillus suis / R. Griffith, ISU / －
Aeromonas hydrophila / R. Griffith, ISU / －
Arcanobacter pyogenes / R.F. Ross, ISU / －
Bacillus “swarming” / R. Griffith, ISU / －
Bacillus anthracis / R. Griffith, ISU / －
Bacillus cereus / R. Griffith, ISU / －
Bacillus cereus / R. Griffith, ISU / －
Bacillus subtilis / R. Griffith, ISU / －
Bibersteinia trehalosi / R. Griffith, ISU / －
Bordetella avium / R. Griffith, ISU / －
Bordetella bronchiseptica / R. Griffith, ISU / －
Bordetella bronchiseptica / R. Griffith, ISU / －
Brachyspira hyodysenteriae / B204 / ATCC / －
Brachyspira innocens / B256 / ATCC / －
Brachyspira intermedia / 51440 / ATCC / －
Brachyspira murdochii / 51284 / ATCC / －
Brachyspira pilosicoli / P43 / ATCC / －
Brucella abortus / Strain 19 / B. Bellaire, ISU / －
Campylobacter jejuni / w7 WT / P. Plummer, ISU / －
Citrobacter diversus / R. Griffith, ISU / －
Citrobacter freundii / R. Griffith, ISU / －
Clostridium difficile / R. Griffith, ISU / －
Clostridium haemolyticum / R. Griffith, ISU / －
Clostridium novyi / R. Griffith, ISU / －
Clostridium perfringens / ISM 2308 / G. Songer, ISU / －
Clostridium perfringens / R. Griffith, ISU / －
Clostridium septicum / ISM 2309 / G. Songer, ISU / －
Clostridium septicum / R. Griffith, ISU / －
Clostridium sordellii / R. Griffith, ISU / －
Clostridium tetani / R. Griffith, ISU / －
Corynebacterium pseudotuberculosis / R. Griffith, ISU / －
Corynebacterium renale / R. Griffith, ISU / －
Edwardsiella ictaluri / R. Griffith, ISU / －
Enterobacter aerogenes / R. Griffith, ISU / －
Enterobacter cloacae / R. Griffith, ISU / －
Enterococcus faecium / R. Griffith, ISU / －
Erysipelothrix rhusiopathiae / R. Griffith, ISU / －
Escherichia coli / 25922 / ATCC / －
Escherichia coli / O157:H7 Sakai / F.C. Minion, ISU / －
Escherichia coli / R. Griffith, ISU / －
Escherichia coli / R. Griffith, ISU / －
Fusobacterium necrophorum / R. Griffith, ISU / －
Fusobacterium necrophorum / R. Griffith, ISU / －
Haemophilus parasuis / R.F. Ross, ISU / －
Klebsiella pneumoniae / R. Griffith, ISU / －
Listeria monocytogenes / R. Griffith, ISU / －
Mannheimia haemolytica / R. Griffith, ISU / －
Micrococcus luteus / R. Griffith, ISU / －
Moraxella bovis / R. Griffith, ISU / －
Morganella morganii / R. Griffith, ISU / －
Pasteurella multocida / R. Griffith, ISU / －
Pasteurella multocida / R. Griffith, ISU / －
Pasturella multocida group A / R. Griffith, ISU / －
Proteus mirabilis / R. Griffith, ISU / －
Proteus vulgaris / R. Griffith, ISU / －
Providencia stuartii / R. Griffith, ISU / －
Providenia rettgeri / R. Griffith, ISU / －
Pseudomonas aeruginosa / R. Griffith, ISU / －
Rhodococcus equi / GFP Clone 7 / B. Bellaire, ISU / －
Rhodococcus equi / R. Griffith, ISU / －
Salmonella enterica serovar Arizonae / R. Griffith, ISU / －
S almonella enterica serovarDublin / R. Griffith, ISU / －
Salmonella enterica serovar
Cholerasuis group C1 / R. Griffith, ISU / －
Salmonella enterica serovar Enteritidis / R. Griffith, ISU / －
Salmonella enterica serovarGallinarum / R. Griffith, ISU / －
Salmonella enterica serovar Pullorum / R. Griffith, ISU / －
Salmonella enterica serovar Typhimurium / R. Griffith, ISU / －
Serratia marcescens / R. Griffith, ISU / －
Staph / R. Griffith, ISU / －
Staphylococcus / R. Griffith, ISU / －
Staphylococcus aureus / R. Griffith, ISU / －
Staphylococcus aureus / R. Griffith, ISU / －
Staphylococcus epidermidis / R. Griffith, ISU / －
Staphylococcus epidermidis / R. Griffith, ISU / －
Staphylococcus hyicus / R. Griffith, ISU / －
Staphylococcus intermedius / R. Griffith, ISU / －
Staphylococcus schleiferi / R. Griffith, ISU / －
Staphylococcus zooepidemicus / R. Griffith, ISU / －
Streptococcus agalactiae / R. Griffith, ISU / －
Streptococcus canis / R. Griffith, ISU / －
Streptococcus dysgalactiae / J. Kinyon, ISU / －
Streptococcus equi / R. Griffith, ISU / －
Streptococcus equisimilis / R. Griffith, ISU / －
Streptococcus porcinus / R. Griffith, ISU / －
Streptococcus suis / R. Griffith, ISU / －
Streptococcus suis type 1 / R. Griffith, ISU / －
Streptococcus suis type 2 / R.F. Ross, ISU / －
Streptococcus uberis / R. Griffith, ISU / －
Yersinia enterocolitica / R. Griffith, ISU / －
Yersinia pestis / R88 / F.C. Minion, ISU / －
Yersinia pseudotuberculosis / R. Griffith, ISU / －

(ISU), Iowa State University; (－), negative result