Supporting Information

Table S1. Crystal growth and soaking conditions

PDB ID / targetDB ID / Growth conditions / Soaking conditions / Soaking time
3K9G / BobuA.01478.a / 3 M NaCl
0.1 M Bis Tris Propane pH 6.5 / 2 M NaCl, 1 M KI
0.1 M Bis Tris Propane pH 6.5 / 1 h at 0.5 M,
1 h at 1 M
3KM3 / AnphA.00973.a / 0.2 M LiCl
0.1 M Tris pH 8.1
20% PEG 3350 / 0.2 M LiCl, 1 M KI
0.1 M Hepes pH 7.0
20% PEG 3350 / 1 h at 0.5 M,
15 min at 1 M
3KW3 / BaheA.00339.a / 0.2 M MgCl2
0.1 M Tris pH 8.5
25% PEG 3350 / 1 M KI
0.1 M Hepes pH 8.0
35% PEG 3350 / 1 h
3LA9 / BupsA.01663.a / 0.1 M MIB pH 4.0
25% PEG 1500 / 1 M KI
0.1 M MIB pH 4.0
35% PEG 1500 / 1 h
3LR0 / BupsA.00863.i / 0.2 M Li2SO4
0.1 M phosphate-citrate pH 4.2
25% PEG 1000 / 1 M KI
0.1 M phosphate-citrate pH 4.2
30% PEG 1000 / 1 h
3LR5 / BupsA.00863.i / 0.2 M NaI
0.1 M Bis Tris Propane pH 6.5
25% PEG 3350 / direct cryo / Co-xtal
3LUZ / BaheA.00759.a / 0.2 M MgCl2
0.1M Na Hepes pH 7.5
30% PEG 400 / 0.75 M KI, 0.2 M MgCl2
0.1M Na Hepes pH 8.2
35% PEG 400 / 1 h
3MD7 / BrabA.11339.a / 0.2 M Na/K tartrate
0.1M Bis Tris Propane pH 8.6
20% PEG 3350 / 0.25 M KI
0.1M Bis Tris Propane pH 8.6
20% PEG 3350 / 5 min
3MEN / BupsA.10154.b / 1.26 M (NH4)2SO4
0.1 M cacodylate pH 6.5 / 0.2 M NaI, 1.26 M (NH4)2SO4
0.1 M cacodylate pH 6.5 / 1 h
3NJB / MysmA.00358.i / 0.2 M NaOAc
0.1M Bis Tris Propane, pH 8.5
20% PEG 3350 / 0.2 M NaOAc, 0.4 M NaI
0.1M Bis Tris Propane, pH 8.5
20% PEG 3350, 15% EG / 10 min
3O2E / BaboA.10365.a / 2 M (NH4)2SO4, 0.2 M NaCl
0.1 M cacodylate pH 6.5 / 2 M (NH4)2SO4, 1 M NaI
0.1  M cacodylate pH 6.5
20% EG / Stepwise
4x5 min
3OIB / MysmA.00247.b / 0.2 M NaKHPO4
0.1M Bis Tris Propane, pH 8.5
20% PEG 3350 / 0.5 M NaI
0.05 M NaKHPO4, pH 7.0
25% PEG 3350, 15% EG / 2 min
3OL3 / MysmA.17112.a / 0.2 M MgCl2
0.1M Na Hepes pH 7.5
30% PEG 400 / 1M NaI, 0.2 M MgCl2
0.1M Na Hepes pH 7.0
35% PEG 400 / 4 min
3P96 / MyavA.01155.a / 0.2 M Mg(formate)2
20% PEG 3350 / 1.0 M KI, 0.2 M Mg(formate)2
20% PEG 3350, 25% EG / 4 min
3PFD / MythA.00185.b / 0.2 M NaCl
0.1 M phosphate-citrate pH 4.2
25% PEG 8000 / 1.0 M NaI
0.1 M phosphate-citrate pH 4.2
15% PEG 8000, 25% PEG 400 / 20 min
3PM6 / CoimA.00345.a / 0.1  M sodium citrate pH 5.0
20% PEG 8000 / 1.0  M NaI
0.1 M sodium citrate pH 5.0
20% PEG 8000, 20% EG / 15 min