Salting the ProteinSurfaceStina Lindman et al.

Supplementary material

Table 1.Thermodynamic parameters derived from urea denaturations including both CD and fluorescence measurements.

[NaCl]pHCm / MG°(H2O) / kJmol-1m / kJmol-1M-1

Low2.53.0 ± 0.18.9 ± 0.52.9 ± 0.1

4.56.6 ± 0.119.4 ± 1.0

7.54.1 ± 0.112.1 ± 0.6

101.4 ± 0.24.0 ± 0.6

0.15M100.5 ± 0.21.5 ± 0.5

2M2.55.0 ± 0.114.7 ± 0.8

4.57.6 ± 0.122.2 ± 1.0

7.54.4 ± 0.112.8 ± 0.7

101.4 ± 0.24.0 ± 0.5

Table 2. Thermodynamic parameters derived from thermal denaturations including both CD and DSC measurements.Cp is derived from a global fit of all thermal denaturations.

[NaCl]pHMethodTm / °CH°(Tm)Cp G°(25°C)

/ kJmol-1/ kJmol-1K-1/ kJmol-1

Low1.5CD50.9 ± 0.2189.0 ± 7.91.8 ± 0.613.2 ± 1.2

2.551.0 ± 0.2189.3 ± 8.113.3 ± 1.2

2.657.7 ± 0.2201.3 ± 6.516.9 ± 1.3

359.1 ± 0.2203.7 ± 6.317.6 ± 1.4

3.570.1 ± 0.3223.6 ± 9.223.8 ± 1.4

3.566.8 ± 0.2217.7 ± 8.022.0 ± 1.4

471.8 ± 0.2226.6 ± 9.624.8 ± 1.4

4.476.1 ± 0.4234.3 ± 12.227.2 ± 1.4

4.576.5 ± 0.3235.0 ± 12.327.4 ± 1.3

575.5 ± 0.3233.3 ± 11.026.9 ± 1.4

5.574.6 ± 0.2231.7 ± 11.626.4 ± 1.4

5.672.2 ± 0.2227.2 ± 9.525.0 ± 1.3

6.169.2 ± 0.2221.9 ± 8.623.3 ± 1.4

6.565.9 ± 0.2216.0 ± 7.121.4 ± 1.4

6.566.3 ± 0.2216.8 ± 7.121.7 ± 1.4

760.8 ± 0.2206.8 ± 6.318.6 ± 1.4

7.555.0 ± 0.2196.5 ± 6.715.4 ± 1.3

7.657.6 ± 0.2201.2 ± 6.316.9 ± 1.4

8.555.8 ± 0.3197.9 ± 6.615.9 ± 1.3

8.650.3 ± 0.2188.1 ± 8.612.9 ± 1.2

9.543.9 ± 0.3176.5 ± 12.59.5 ± 1.1

9.541.8 ± 0.3172.8 ± 13.28.4 ± 1.0

10.434.2 ± 0.9159.0 ± 19.24.5 ± 0.8

10.431.1 ± 1.3153.5 ± 22.63.0 ± 0.8

11.4*---

11.5*---

4.5DSC70.2 ± 0.2223.7 ± 8.423.8 ± 1.4

7.549.8 ± 0.4187.2 ± 7.912.6 ± 1.2

0.15M1.6CD52.6 ± 0.2192.0 ± 7.414.1 ± 1.3

2.555.1 ± 0.4196.6 ± 7.015.5 ± 1.3

2.555.3 ± 0.2197.0 ± 7.115.6 ± 1.3

358.4 ± 0.2202.6 ± 6.317.3 ± 1.4

3.567.0 ± 0.4218.0 ± 7.222.1 ± 1.3

3.565.0 ± 0.2214.5 ± 7.221.0 ± 1.4

468.6 ± 0.2220.8 ± 7.822.9 ± 1.3

4.470.6 ± 0.2224.4 ± 9.824.1 ± 1.3

4.571.5 ± 0.4226.1 ± 9.724.6 ± 1.4

570.0 ± 0.2223.3 ± 9.223.7 ± 1.4

5.566.2 ± 0.3216.6 ± 7.221.6 ± 1.4

5.562.7 ± 0.2210.2 ± 6.419.6 ± 1.3

663.2 ± 0.2211.2 ± 6.319.9 ± 1.4

6.558.4 ± 0.3202.5 ± 6.217.3 ± 1.3

6.558.2 ± 0.2202.1 ± 6.117.2 ± 1.4

755.3 ± 0.2197.0 ± 6.815.6 ± 1.4

7.549.8 ± 0.3187.0 ± 8.112.6 ± 1.1

7.553.6 ± 0.2193.9 ± 7.214.6 ± 1.2

8.547.0 ± 0.5182.0 ± 10.011.1 ± 1.2

8.547.1 ± 0.2182.3 ± 9.611.2 ± 1.1

9.539.9 ± 0.4169.4 ± 13.77.4 ± 0.9

9.539.8 ± 0.4169.1 ± 14.17.4 ± 0.9

9.539.9 ± 0.4169.3 ± 14.57.4 ± 1.0

10.5**23.6140.1-0.6

10.526.1 ± 3.0144.5 ± 29.20.5 ± 0.8

11.5*---

11.5*---

4.5DSC65.9 ± 0.3216.0 ± 6.921.5 ± 1.5

7.549.4 ± 0.4186.4 ± 7.412.4 ± 1.1

2M1.5CD68.7 ± 0.3221.0 ± 8.423.0 ± 1.4

268.2 ± 0.2220.1 ± 8.622.7 ± 1.3

2.569.1 ± 0.2221.8 ± 8.723.3 ± 1.4

2.568.4 ± 0.2220.6 ± 8.422.9 ± 1.4

371.3 ± 0.2225.6 ± 10.024.5 ± 1.4

3.574.7 ± 0.3231.7 ± 11.326.4 ± 1.4

3.571.7 ± 0.2226.4 ± 10.024.7 ± 1.4

472.0 ± 0.2226.9 ± 10.124.9 ± 1.4

4.572.2 ± 0.2227.3 ± 10.125.0 ± 1.4

4.572.2 ± 0.2227.3 ± 10.225.0 ± 1.4

4.969.8 ± 0.2223.1 ± 8.723.7 ± 1.3

5.568.8 ± 0.2221.2 ± 8.723.1 ± 1.4

5.565.9 ± 0.2216.0 ± 7.721.4 ± 1.5

662.8 ± 0.2210.5 ± 6.419.7 ± 1.3

6.564.7 ± 0.3213.8 ± 7.020.8 ± 1.4

6.561.2 ± 0.2207.5 ± 6.318.8 ± 1.4

7.159.4 ± 0.2204.3 ± 6.017.8 ± 1.3

7.558.4 ± 0.2202.6 ± 6.117.3 ± 1.3

7.558.2 ± 0.3202.1 ± 6.417.2 ± 1.4

8.453.4 ± 0.2193.5 ± 7.314.5 ± 1.3

8.552.4 ± 0.3191.8 ± 7.914.0 ± 1.3

9.446.6 ± 0.3181.3 ± 10.210.9 ± 1.1

9.546.9 ± 0.3181.9 ± 10.311.1 ± 1.2

10.534.7 ± 0.7160.0 ± 17.54.8 ± 0.8

11.5*---

4.5DSC68.2 ± 0.3220.1 ± 6.622.7 ± 1.5

7.555.9 ± 0.3198.1 ± 6.115.9 ± 1.3

* Sample is denatured at whole temperature range.

** Errors not shown due to non-complete denaturation curve.

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