Salting the ProteinSurfaceStina Lindman et al.
Supplementary material
Table 1.Thermodynamic parameters derived from urea denaturations including both CD and fluorescence measurements.
[NaCl]pHCm / MG°(H2O) / kJmol-1m / kJmol-1M-1
Low2.53.0 ± 0.18.9 ± 0.52.9 ± 0.1
4.56.6 ± 0.119.4 ± 1.0
7.54.1 ± 0.112.1 ± 0.6
101.4 ± 0.24.0 ± 0.6
0.15M100.5 ± 0.21.5 ± 0.5
2M2.55.0 ± 0.114.7 ± 0.8
4.57.6 ± 0.122.2 ± 1.0
7.54.4 ± 0.112.8 ± 0.7
101.4 ± 0.24.0 ± 0.5
Table 2. Thermodynamic parameters derived from thermal denaturations including both CD and DSC measurements.Cp is derived from a global fit of all thermal denaturations.
[NaCl]pHMethodTm / °CH°(Tm)Cp G°(25°C)
/ kJmol-1/ kJmol-1K-1/ kJmol-1
Low1.5CD50.9 ± 0.2189.0 ± 7.91.8 ± 0.613.2 ± 1.2
2.551.0 ± 0.2189.3 ± 8.113.3 ± 1.2
2.657.7 ± 0.2201.3 ± 6.516.9 ± 1.3
359.1 ± 0.2203.7 ± 6.317.6 ± 1.4
3.570.1 ± 0.3223.6 ± 9.223.8 ± 1.4
3.566.8 ± 0.2217.7 ± 8.022.0 ± 1.4
471.8 ± 0.2226.6 ± 9.624.8 ± 1.4
4.476.1 ± 0.4234.3 ± 12.227.2 ± 1.4
4.576.5 ± 0.3235.0 ± 12.327.4 ± 1.3
575.5 ± 0.3233.3 ± 11.026.9 ± 1.4
5.574.6 ± 0.2231.7 ± 11.626.4 ± 1.4
5.672.2 ± 0.2227.2 ± 9.525.0 ± 1.3
6.169.2 ± 0.2221.9 ± 8.623.3 ± 1.4
6.565.9 ± 0.2216.0 ± 7.121.4 ± 1.4
6.566.3 ± 0.2216.8 ± 7.121.7 ± 1.4
760.8 ± 0.2206.8 ± 6.318.6 ± 1.4
7.555.0 ± 0.2196.5 ± 6.715.4 ± 1.3
7.657.6 ± 0.2201.2 ± 6.316.9 ± 1.4
8.555.8 ± 0.3197.9 ± 6.615.9 ± 1.3
8.650.3 ± 0.2188.1 ± 8.612.9 ± 1.2
9.543.9 ± 0.3176.5 ± 12.59.5 ± 1.1
9.541.8 ± 0.3172.8 ± 13.28.4 ± 1.0
10.434.2 ± 0.9159.0 ± 19.24.5 ± 0.8
10.431.1 ± 1.3153.5 ± 22.63.0 ± 0.8
11.4*---
11.5*---
4.5DSC70.2 ± 0.2223.7 ± 8.423.8 ± 1.4
7.549.8 ± 0.4187.2 ± 7.912.6 ± 1.2
0.15M1.6CD52.6 ± 0.2192.0 ± 7.414.1 ± 1.3
2.555.1 ± 0.4196.6 ± 7.015.5 ± 1.3
2.555.3 ± 0.2197.0 ± 7.115.6 ± 1.3
358.4 ± 0.2202.6 ± 6.317.3 ± 1.4
3.567.0 ± 0.4218.0 ± 7.222.1 ± 1.3
3.565.0 ± 0.2214.5 ± 7.221.0 ± 1.4
468.6 ± 0.2220.8 ± 7.822.9 ± 1.3
4.470.6 ± 0.2224.4 ± 9.824.1 ± 1.3
4.571.5 ± 0.4226.1 ± 9.724.6 ± 1.4
570.0 ± 0.2223.3 ± 9.223.7 ± 1.4
5.566.2 ± 0.3216.6 ± 7.221.6 ± 1.4
5.562.7 ± 0.2210.2 ± 6.419.6 ± 1.3
663.2 ± 0.2211.2 ± 6.319.9 ± 1.4
6.558.4 ± 0.3202.5 ± 6.217.3 ± 1.3
6.558.2 ± 0.2202.1 ± 6.117.2 ± 1.4
755.3 ± 0.2197.0 ± 6.815.6 ± 1.4
7.549.8 ± 0.3187.0 ± 8.112.6 ± 1.1
7.553.6 ± 0.2193.9 ± 7.214.6 ± 1.2
8.547.0 ± 0.5182.0 ± 10.011.1 ± 1.2
8.547.1 ± 0.2182.3 ± 9.611.2 ± 1.1
9.539.9 ± 0.4169.4 ± 13.77.4 ± 0.9
9.539.8 ± 0.4169.1 ± 14.17.4 ± 0.9
9.539.9 ± 0.4169.3 ± 14.57.4 ± 1.0
10.5**23.6140.1-0.6
10.526.1 ± 3.0144.5 ± 29.20.5 ± 0.8
11.5*---
11.5*---
4.5DSC65.9 ± 0.3216.0 ± 6.921.5 ± 1.5
7.549.4 ± 0.4186.4 ± 7.412.4 ± 1.1
2M1.5CD68.7 ± 0.3221.0 ± 8.423.0 ± 1.4
268.2 ± 0.2220.1 ± 8.622.7 ± 1.3
2.569.1 ± 0.2221.8 ± 8.723.3 ± 1.4
2.568.4 ± 0.2220.6 ± 8.422.9 ± 1.4
371.3 ± 0.2225.6 ± 10.024.5 ± 1.4
3.574.7 ± 0.3231.7 ± 11.326.4 ± 1.4
3.571.7 ± 0.2226.4 ± 10.024.7 ± 1.4
472.0 ± 0.2226.9 ± 10.124.9 ± 1.4
4.572.2 ± 0.2227.3 ± 10.125.0 ± 1.4
4.572.2 ± 0.2227.3 ± 10.225.0 ± 1.4
4.969.8 ± 0.2223.1 ± 8.723.7 ± 1.3
5.568.8 ± 0.2221.2 ± 8.723.1 ± 1.4
5.565.9 ± 0.2216.0 ± 7.721.4 ± 1.5
662.8 ± 0.2210.5 ± 6.419.7 ± 1.3
6.564.7 ± 0.3213.8 ± 7.020.8 ± 1.4
6.561.2 ± 0.2207.5 ± 6.318.8 ± 1.4
7.159.4 ± 0.2204.3 ± 6.017.8 ± 1.3
7.558.4 ± 0.2202.6 ± 6.117.3 ± 1.3
7.558.2 ± 0.3202.1 ± 6.417.2 ± 1.4
8.453.4 ± 0.2193.5 ± 7.314.5 ± 1.3
8.552.4 ± 0.3191.8 ± 7.914.0 ± 1.3
9.446.6 ± 0.3181.3 ± 10.210.9 ± 1.1
9.546.9 ± 0.3181.9 ± 10.311.1 ± 1.2
10.534.7 ± 0.7160.0 ± 17.54.8 ± 0.8
11.5*---
4.5DSC68.2 ± 0.3220.1 ± 6.622.7 ± 1.5
7.555.9 ± 0.3198.1 ± 6.115.9 ± 1.3
* Sample is denatured at whole temperature range.
** Errors not shown due to non-complete denaturation curve.
1