Suppl. Table 1 Score and Matched Peptides of the Identified Proteins Using Nano-LC MS/MS

Suppl. Table 1 Score and matched peptides of the identified proteins using nano-LC MS/MS from liver proteome (ab15191, SC1746, SC7951) and RAW 264.7 proteome (33/Cox-2) after 2DE blot using different COX-2 antibodies.

Mascot protein name
(Uni-Prot Acc. Number) / Spot Label / Score / Number of matched peptides / Sequencesofmatchedpeptides
COX-2 Abcam (ab15191), rabbit polyclonal
DnaJ homolog subfamily C member 3
Q9R0T3 / DNJC3_RAT / 109 / 7/7 / K.MDFTAAR.L + Oxidation (M)
K.FIDIAAAK.E
K.AALPDLTR.V
K.EVLSDPEMR.R + Oxidation (M)
K.LIGSAEELIR.D
R.RATVFLAMGK.S + Oxidation (M)
R.LAEAEDDFKK.V
Alpha-enolase
P04764 / ENOA_RAT / 302 / 17/16 / K.YDLDFK.S
K.YNQILR.I
K.EALELLK.S
K.TIAPALVSK.K
R.IEEELGSK.A
K.LNVVEQEK.I
K.SCNCLLLK.V + 2 Carbamidomethyl (C)
R.AGKYDLDFK.S
K.KLNVVEQEK.I
R.IGAEVYHNLK.N
R.GNPTVEVDLYTAK.G
R.YITPDQLADLYK.S
K.FGANAILGVSLAVCK.A + Carbamidomethyl (C)
K.VNQIGSVTESLQACK.L + Carbamidomethyl (C)
R.AAVPSGASTGIYEALELR.D
R.AAVPSGASTGIYEALELR.D
K.AGYTDQVVIGMDVAASEFYR.A + Oxidation (M)
D-3-phosphoglycerate dehydrogenase
O08651 / SERA_RAT / 111 / 7/7 / K.TLGILGLGR.I
R.GQPLLLFR.A
K.QADVNLVNAK.L
R.GGIVDEGALLR.A
K.VTADVINAAEK.L
K.ILQDGGLQVVEK.Q
K.GTIQVVTQGTSLK.N
Arginase-1
P07824 / ARGI1_RAT / 583 / 19/15 / K.ETEYNVR.D
K.DIVYIGLR.D
R.GGVEKGPAALR.K
K.YFSMTEVDK.L
K.YFSMTEVDK.L + Oxidation (M)
K.LKETEYNVR.D
K.LKETEYNVR.D
R.DVDPGEHYIIK.T
R.EGLYITEEIYK.T
K.VMEETFSYLLGR.K
K.VMEETFSYLLGR.K + Oxidation (M)
K.VMEETFSYLLGR.K + Oxidation (M)
K.ANEQLAAVVAETQK.N
K.ANEQLAAVVAETQK.N
K.YFSMTEVDKLGIGK.V + Oxidation (M)
R.TVNTAVALTLSCFGTK.R + Carbamidomethyl (C)
R.TVNTAVALTLSCFGTK.R + Carbamidomethyl (C)
K.TGLLSGLDIMEVNPTLGK.T + Oxidation (M)
R.DHGDLAFVDVPNDSPFQIVK.N
Cystathionine gamma-lyase
P18757 / CGL_RAT / 685 / 16/12 / K.AVAALDGAK.H
R.VASEFGLK.I
R.FLESNPR.V
R.FLESNPR.V
K.ISFVDCSK.T + Carbamidomethyl (C)
R.RVASEFGLK.I
R.ATLGISDTLIR.L
R.ATLGISDTLIR.L
K.LLEAAITPQTK.L
K.LLEAAITPQTK.L
K.DLLEDLGQALK.A
K.GTLQHAQVFLK.N
R.AVVLPISLATTFK.Q
R.AVVLPISLATTFK.Q
K.LVWIETPTNPTLK.L
R.LSVGLEDEKDLLEDLGQALK.A
Fructose-bisphosphate aldolase B
P00884 / ALDOB_RAT / 404 / 10/8 / K.LSFSYGR.A
K.ELSEIAQR.I
K.ELSEIAQR.I
K.KELSEIAQR.I
K.ATQEAFMKR.A + Oxidation (M)
K.LDQGGAPLAGTNK.E
R.ALQASALAAWGGK.A
K.ETTIQGLDGLSER.C
K.YTPEQVAMATVTALHR.T + Oxidation (M)
K.YTPEQVAMATVTALHR.T + Oxidation (M)
COX-2 Santa Cruz (SC1746), goat polyclonal
Vimentin
P31000 / VIME_RAT / 178 / 16/9 / R.EYQDLLNVK.M
K.ILLAELEQLK.G
K.NLQEAEEWYK.S
R.QVQSLTCEVDALK.G + Carbamidomethyl (C)
R.QVQSLTCEVDALK.G + Carbamidomethyl (C)
R.TYSLGSALRPSTSR.S
R.KVESLQEEIAFLK.K
R.KVESLQEEIAFLK.K
R.KVESLQEEIAFLK.K
K.ILLAELEQLKGQGK.S
K.ILLAELEQLKGQGK.S
K.ILLAELEQLKGQGK.S
R.ISLPLPNFSSLNLR.E
R.ISLPLPNFSSLNLR.E
R.QVQSLTCEVDALKGTNESLER.Q + Carbamidomethyl (C)
R.QVQSLTCEVDALKGTNESLER.Q + Carbamidomethyl (C)
COX-2 Santa Cruz (SC7951), rabbit polyclonal
Procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Q5U367 / PLOD3_RAT / 60 / 3/2 / R.TLPGGQPPPR.V
R.SEDYVELVQR.K
R.SEDYVELVQR.K
Heat shock 70 kDa protein 1A/1B
Q07439 / HSP71_RAT / 77 / 11/6 / K.SAVEDEGLK.G
K.LLQDFFNGR.D
K.LLQDFFNGR.D
K.DAGVIAGLNVLR.I
K.DAGVIAGLNVLR.I
R.TTPSYVAFTDTER.L
R.TTPSYVAFTDTER.L
R.TTPSYVAFTDTER.L
K.ATAGDTHLGGEDFDNR.L
R.IINEPTAAAIAYGLDR.T
R.IINEPTAAAIAYGLDR.T
COX-2 BD Transduction Laboratories (33/Cox-2), mouse monoclonal
Chain A, Cyclooxygenase-2 (Prostaglandin Synthase-2) Complexed With A Non-Selective Inhibitor, Flurbiprofen
Q05769 / PGH2_MOUSE / 159 / 7/7 / ANPCCSNPCQNR
GCPFTSFNVQDPQPTK
GECMSTGFDQYK
LDDINPTVLIK
LILIGETIK
NVPIAVQAVAK
TATINASASHSR

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