SUPPLEMENTARY RESOURCES

  1. PERMANOVA (Permutational MANOVA)

Test MBT (monobutyltin)

NameAbbrev.TypeLevels

seasonseFixed 2

stationstFixed 12

PERMANOVA table of results

Unique

Sourcedf SS MSPseudo-FP(perm) perms

se 12786,62786,6 6,6812 0,0001 9945

st11 392883571,6 8,5632 0,0001 9899

sexst11 320932917,5 6,9951 0,0001 9895

Res48 20020417,08

Total71 94187

PAIR-WISE TESTS

Term 'sexst' for pairs of levels of factor 'season'

Within level 'SG1' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w5,6441 0,0974 100,0014

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d80,491

w29,42780,095

Within level 'SG2' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w5,6572 0,0992 100,0028

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d93,099

w63,43588,464

Within level 'SG3' of factor 'station'

Unique

Groups tP(perm) permsP(MC)

d, w7,7433 0,1038 100,001

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d97,636

w71,22391,909

Within level 'SG4' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,91231 0,4 100,4235

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d80,391

w77,64169,132

Within level 'SG5' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,7456 0,1002 100,0277

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d97,053

w47,78263,335

Within level 'SG6' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,95911 0,4017 100,3945

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d 90,26

w88,14683,138

Within level 'SG7' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w3,2268 0,0963 100,0277

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d95,061

w73,46482,447

Within level 'SG8' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,84859 0,6992 100,4448

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d87,504

w83,75875,013

Within level 'SG9' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,96466 0,392 100,3937

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d92,525

w76,79670,902

Within level 'SG10' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w4,37 0,1068 100,0024

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d90,295

w15,01257,778

Within level 'SG11' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,818 0,103 100,1274

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d86,703

w62,55658,401

Within level 'SG12' of factor 'station'

Unique

Groups tP(perm) permsP(MC)

d, w1,898 0,0965 10 0,06

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d91,855

w14,10911,852

Test DBT (Dibutyltin)

Factors

NameAbbrev.TypeLevels

seasonseFixed 2

stationstFixed 12

PERMANOVA table of results

Unique

Sourcedf SS MSPseudo-FP(perm) perms

se 12551,82551,8 9,0892 0,0001 9948

st11 376933426,6 12,205 0,0001 9911

sexst11 192931753,9 6,2472 0,0001 9889

Res44 12353280,75

Total67 69737

PAIR-WISE TESTS

Term 'sexst' for pairs of levels of factor 'season'

Within level 'SG1' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,9313 0,1016 100,0904

Denominators

GroupsDenominatorDen.df

d, w1*Res 3

Average Similarity between/within groups

d w

d 0

w11,29786,436

Within level 'SG2' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w5,2549 0,0991 100,0034

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d86,625

w48,07484,702

Within level 'SG3' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w4,9486 0,0953 100,0062

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d97,007

w68,17587,132

Within level 'SG4' of factor 'station'

Unique

Groups tP(perm) permsP(MC)

d, w2,7988 0,1017 100,034

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d83,224

w53,78969,443

Within level 'SG5' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,68283 0,7938 100,5461

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d94,212

w88,71280,074

Within level 'SG6' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w9,9664E-2 1 100,9484

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d 88,56

w89,85186,817

Within level 'SG7' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w5,9467 0,1063 100,0024

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d92,412

w66,44489,54

Within level 'SG8' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,297 0,201 100,2544

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d92,325

w88,54984,438

Within level 'SG9' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,7566 0,3001 100,1514

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d92,808

w73,17971,085

Within level 'SG10' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w9,9149 0,1018 70,0011

Denominators

GroupsDenominatorDen.df

d, w1*Res 3

Average Similarity between/within groups

d w

d99,399

w49,71986,486

Within level 'SG11' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,33208 1 100,8124

Denominators

GroupsDenominatorDen.df

d, w1*Res 3

Average Similarity between/within groups

d w

d72,427

w83,82387,023

Within level 'SG12' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,8597 0,1008 100,1573

Denominators

GroupsDenominatorDen.df

d, w1*Res 3

Average Similarity between/within groups

d w

d96,225

w66,16553,097

Test TBT (Tributyltin)

Factors

NameAbbrev.TypeLevels

stationstFixed 12

seasonseFixed 2

PERMANOVA table of results

Unique

Sourcedf SS MSPseudo-FP(perm) perms

st11 27,476 2,4978 4,9625 0,0002 9914

se 10,123950,12395 0,24624 0,6299 9839

stxse11 19,239 1,749 3,4748 0,0016 9937

Res48 24,1610,50335

Total71 71

PAIR-WISE TESTS

Term 'stxse' for pairs of levels of factor 'season'

Within level 'SG1' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w3,2356 0,0959 40,0322

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d 0

w0,818640,58406

Within level 'SG2' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,6262 0,1021 100,0596

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d0,26809

w 2,69532,3458

Within level 'SG3' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,2948 0,3044 100,2724

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d 2,748

w1,76810,56491

Within level 'SG4' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,42428 0,7026 100,6977

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d0,78513

w 1,03411,5798

Within level 'SG5' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,41217 0,6931 40,7046

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d0,45959

w0,373420,52661

Within level 'SG6' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,9415 0,3973 20,1238

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

dw

d1,2256

w1,07240

Within level 'SG7' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w3,0628E-2 1 70,9734

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d0,57449

w 0,37980,36384

Within level 'SG8' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,12798 0,9006 90,9063

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d0,41172

w0,271290,28724

Within level 'SG9' of factor 'station'

Unique

Groups tP(perm) permsP(MC)

d, w2,3065 0,1018 100,082

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d0,24894

w0,880880,84258

Within level 'SG10' of factor 'station'

Unique

Groups tP(perm) permsP(MC)

d, wDenominator is 0

Denominators

GroupsDenominatorDen.df

d, w1*Res 0

Average Distance between/within groups

dw

d0

w00

Within level 'SG11' of factor 'station'

Unique

Groups tP(perm) permsP(MC)

d, wDenominator is 0

Denominators

GroupsDenominatorDen.df

d, w1*Res 0

Average Distance between/within groups

dw

d0

w00

Within level 'SG12' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w23,568 0,0937 40,0003

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

dw

d4,7874E-2

w 0,517040

Test N (densities)

Factors

NameAbbrev.TypeLevels

seasonseFixed 2

stationstFixed 12

PERMANOVA table of results

Unique

Sourcedf SS MSPseudo-FP(perm) perms

se 1 2882,22882,2 4,3747 0,0067 9940

st11 535404867,2 7,3877 0,0001 9921

sexst11 244022218,4 3,3671 0,0001 9894

Res48 31624658,83

Total711,1245E5

PAIR-WISE TESTS

Term 'sexst' for pairs of levels of factor 'season'

Within level 'SG1' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w4,2462 0,0988 100,0094

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d91,408

w66,01685,7

Within level 'SG2' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,9314 0,5094 100,4165

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d53,733

w55,63555,113

Within level 'SG3' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,5862 0,6082 100,6179

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d80,974

w78,39665,544

Within level 'SG4' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w3,9458 0,097 70,0059

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d86,667

w34,46266,209

Within level 'SG5' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,8493 0,1021 100,1118

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d91,051

w53,31352,829

Within level 'SG6' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,67355 0,9012 100,5877

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d93,252

w73,89953,125

Within level 'SG7' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w3,3479 0,0934 100,0151

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d80,968

w48,61473,462

Within level 'SG8' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,62865 0,8979 100,6522

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d79,483

w64,65741,923

Within level 'SG9' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,4619 0,1001 100,0372

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d90,216

w42,95654,367

Within level 'SG10' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,0496 0,3042 100,3538

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d68,942

w59,82654,571

Within level 'SG11' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w3,6628 0,1033 100,0179

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d86,207

w71,44189,126

Within level 'SG12' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,0818 0,0998 100,0741

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d73,823

w36,36343,247

Test S (Genera)

Factors

NameAbbrev.TypeLevels

seasonseFixed 2

stationstFixed 12

PERMANOVA table of results

Unique

Sourcedf SS MSPseudo-FP(perm) perms

se 188,88988,889 5,1323 0,0305 9753

st11706,2864,207 3,7072 0,0012 9922

sexst11820,1174,556 4,3047 0,0003 9942

Res48831,3317,319

Total712446,6

PAIR-WISE TESTS

Term 'sexst' for pairs of levels of factor 'season'

Within level 'SG1' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,71207 0,4921 80,5115

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

dw

d4,6667

w3,77784

Within level 'SG2' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,64416 0,4922 80,5528

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

dw

d 8

w6,66678

Within level 'SG3' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,8779 0,4067 50,4248

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d6,6667

w5,66675,3333

Within level 'SG4' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w4,9015 0,1014 100,0081

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d1,3333

w10,3334,6667

Within level 'SG5' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,97435 0,4048 70,3905

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d 4

w6,777810,667

Within level 'SG6' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,4623 0,2007 80,0665

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d5,3333

w8,22224,6667

Within level 'SG7' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w4,7181 0,1026 90,0087

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d 2

w10,6674,6667

Within level 'SG8' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,48507 0,7984 60,6599

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

dw

d 2

w3,55566

Within level 'SG9' of factor 'station'

Unique

Groups tP(perm) permsP(MC)

d, w3,0542 0,0999 90,038

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d 4

w8,33334,6667

Within level 'SG10' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,9292 0,3002 60,1243

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d3,3333

w6,55566,6667

Within level 'SG11' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,4282 0,202 50,0729

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

dw

d4,6667

w8,33336

Within level 'SG12' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w5,9696 0,1034 90,0049

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Distance between/within groups

d w

d3,3333

w9,33331,3333

Test H’

Factors

NameAbbrev.TypeLevels

seasonseFixed 2

stationstFixed 12

PERMANOVA table of results

Unique

Sourcedf SS MSPseudo-FP(perm) perms

se 1 502,1 502,1 2,1906 0,1218 9943

st11 126711151,9 5,0257 0,0001 9934

sexst119366,5 851,5 3,7149 0,0002 9923

Res48 11002229,21

Total71 33542

PAIR-WISE TESTS

Term 'sexst' for pairs of levels of factor 'season'

Within level 'SG1' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,3944 0,3072 100,2348

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d95,412

w94,27894,639

Within level 'SG2' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,737 0,2909 100,1588

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d97,773

w92,11391,543

Within level 'SG3' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,76542 0,4996 100,4931

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d93,553

w86,81980,418

Within level 'SG4' of factor 'station'

Unique

Groups tP(perm) permsP(MC)

d, w1,9305 0,2018 100,123

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d87,871

w88,02893,642

Within level 'SG5' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,84661 0,5032 100,4532

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d95,785

w 93,0289,503

Within level 'SG6' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,3227 0,1001 100,0683

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d76,996

w71,48291,785

Within level 'SG7' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,7153 0,1008 100,0366

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d57,385

w44,57886,535

Within level 'SG8' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w0,32988 0,8955 100,8065

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d 85,21

w83,56272,572

Within level 'SG9' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w2,5882 0,0985 100,0409

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d89,253

w59,37966,994

Within level 'SG10' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w4,3618 0,106 100,0112

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d98,052

w81,35891,479

Within level 'SG11' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,0994 0,294 100,3348

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d48,012

w64,09979,788

Within level 'SG12' of factor 'station'

Unique

Groups tP(perm) perms P(MC)

d, w1,3603 0,2989 100,2495

Denominators

GroupsDenominatorDen.df

d, w1*Res 4

Average Similarity between/within groups

d w

d 97,58

w89,78284,951

  1. Interaction between nematode communities and tributyltin compounds

DISTLM test (Distance based linear models):

Test for environmental variables:

VARIABLES

1. pH

2. Sal(PSU)

3. ORP (mV)

4. TOC(%)

5. MBT (ng/g)

6. DBT (ng/g)

7. TBT (ng/g)

MARGINAL TESTS

VariableSS(trace)Pseudo-F P Prop.

pH 17786 6,16540,00018,0948E-2

Sal(PSU) 40053 15,6050,0001 0,18229

ORP (mV) 20113 7,05350,0001 9,154E-2

TOC(%) 6019,2 1,97160,05242,7395E-2

MBT (ng/g) 4438,3 1,4431 0,171 2,02E-2

DBT (ng/g) 6775,9 2,22740,03613,0839E-2

TBT (ng/g) 3951,71,282 0,2331 1,7985E-2

res.df: 70

NO STARTING TERMS

SEQUENTIAL TESTS

VariableAdj R^2SS(trace)Pseudo-F P Prop. Cumul.res.df

+Sal(PSU)0,17061 40053 15,6050,0001 0,182290,18229 70

+ORP (mV)0,20967 10908 4,45970,00034,9643E-20,23193 69

+pH0,24842 10601 4,5580,00064,8249E-20,28018 68

+DBT (ng/g)0,27688 8226,8 3,6763 0,0013,7442E-20,31762 67

+TOC(%)0,28701 4305 1,95110,05221,9593E-20,33722 66

+TBT (ng/g)0,29309 3431 1,56840,1269 1,5615E-20,35283 65

Best solution

Adj R^2 R^2 RSS No.Vars Selections

0,29309 0,35283 1,422E5 6 1-4,6,7

Percentage of variation explained by individual axes

% explained variation % explained variation

out of fitted modelout of total variation

AxisIndividual Cumulative Individual Cumulative

1 56,37 56,37 19,89 19,89

2 24,82 81,19 8,76 28,65

3 9,62 90,81 3,39 32,04

4 6,14 96,95 2,17 34,21

5 1,99 98,94 0,7 34,91

6 1,06 100 0,37 35,28

  1. Feeding types structure

Figure 1. Percentage of nematode feeding types structure (1A: selective deposit feeders, 1B: nonselective deposit feeders, 2A: epistrate feeders, 2B: predator and omnivorous feeders) in each station in dry (D) and wet (W) season.

  1. List of nematode in dry and wet seasons

Table 1. list of nematode genera in dry and wet season

List of genera in dry season / List of genera in wet season
Achromadora / Achromadora
Adoncholaimus / Acrobeloides
Anguimonhystera / Acrostichus
Anoplostoma / Adoncholaimus
Aphanolaimus / Afrodorylaimus
Aphanonchus / Alaimus
Aphelenchus / Amphimonhystrella
Calolaimus / Anguimonhystera
Chiloplectus / Aphanonchus
Chromadorina / Aphelenchoides
Chromadorita / Aphelenchus
Chronogaster / Aporcelaimellus
Chrysonema / Belondira
Cobbonchus / Boreolaimus
Comesa / Caenorhabditis
Coomansus / Calolaimus
Crocodorylaimus / Campylaimus
Cryptonchus / Chromadorita
Daptonema / Chronogaster
Desmodora / Chrysonema
Dichromadora / Clarkus
Diplogaster / Cobbonchus
Diplolaimella / Coomansus
Diplolaimelloides / Crassogula
Dorylaimus / Cryptonchus
Drepanodorylaimus / Curviditis
Eucephalobus / Daptonema
Eumonhystera / Desmodora
Filitonchus / Diplogasteroides
Geomonhystera / Diplogastrellus
Halalaimus / Diplolaimella
Hofmaenneria / Diplolaimelloides
Hopperia / Diploscapter
Idiodorylaimus / Domorganus
Ironus / Dorylaimus
Laimydorus / Drepanodorylaimus
Linhystera / Ethmolaimus
Mesodorylaimus / Eudorylaimus
Mesorhabditis / Eumonhystera
Metacomesoma / Geomonhystera
Metadesmolaimus / Goffartia
Metalinhomoeus / Halalaimus
Monhystera / Heterocephalobus
Mononchoides / Iotonchus
Mononchulus / Ironus
Mononchus / Ischiodorylaimus
Mylonchulus / Laimydorus
Neochromadora / Leptolaimus
Neotobrilus / Linhystera
Nygolaimus / Mesodorylaimus
Oionchus / Metacomesoma
Oncholaimus / Metalinhomoeus
Oxydirus / Microlaimus
Oxystomina / Monhystera
Panagrolaimus / Monhystrella
Paracanthonchus / Mononchoides
Paracyatholaimus / Mononchulus
Paraphanolaimus / Mononchus
Paraplectonema / Mylonchulus
Parodontophora / Neotobrilus
Paroigolaimella / Nygolaimus
Plectus / Odontolaimus
Prochromadora / Oncholaimellus
Prodesmodora / Oncholaimus
Protorhabditis / Oxydirus
Punctodora / Oxystomina
Rhabditoides / Panagrellus
Rhabdolaimus / Panagrolaimus
Rogerus / Paramphidelus
Sabatieria / Paraphanolaimus
Semitobrilus / Paraplectonema
Sphaerolaimus / Parodontophora
Sphaerotheristus / Pierrickia
Spilophorella / Plectus
Subsphaerolaimus / Practinolaimus
Teratocephalus / Prismatolaimus
Terschellingia / Prochromadora
Thalassomonhystera / Prodesmodora
Theristus / Prodontorhabditis
Thornia / Prodorylaimus
Tobrilus / Protorhabditis
Tridentulus / Pseudolella
Tripyla / Punctodora
Tripyloides / Retrotheristhus
Trissonchulus / Rhabditoides
Tylendorhynchus / Rhabdolaimus
Udonchus / Sabatieria
Viscosia / Scleramphidelus
Semitobrilus
Sinanema
Sphaerotheristus
Terschellingia
Thalassomonhystera
Theristus
Thornia
Tobrilus
Tridentulus
Tripyla
Trischistoma
Trissonchulus
Udonchus
Viscosia
  1. Tables of pairwise comparison:

Table 2.The tables show the results of the pairwise comparisons for each of the BT components: (a) monobutyltin (MBT), (b) dibutyltin (DBT) and (c) tributyltin (TBT) concentrations in the dry season (D) and wet season (W) in the Sai Gon River. Significant pairwise comparisons between seasons (significant differences < 0,05 indicated with *) and stations are shown (stations that differ from the station as indicated in the first row are listed).

1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12
* / * / * / * / * / *
D
2
3
4
5
6
7
8
9
10
11
12 / W
6
10
11
12 / D
3
5
6
9
10 / W
5
10
11
12 / D
4
5
6
7
8
9
10
11
12 / W
5
10
11
12 / D
10 / W
10
11
12 / D
7
8
9
10
11
12 / W
6
7
10 / D
7
8
9
10
11
12 / W
10
11
12 / D
9
10 / W
10
11
12 / D / W
10
11
12 / D
10
11
12 / W
10
11
12 / D
12 / W / D / W / D / W

(a)

1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12
* / * / * / * / *
D
1
2
3
4
5
6
7
8
9
10
12 / W
8
10
11
12 / D
3
4
5
6
9
12 / W
8
10
11
12 / D
4
5
6
7
8
9
10
11
12 / W
8
10
11
12 / D
5
7
8
10
11 / W
8
10
11
12 / D
7
8
9
10
11
12 / W
10
11
12 / D
7
8
9
10
11
12 / W
8
10
11
12 / D
9
10 / W
8
10
11
12 / D
9
10
12 / W
10 / D
10
11
12 / W
10
11
12 / D
12 / W / D / W / D / W

(b)

1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12
* / *
D
4
9
12 / W
6
10
11
12 / D
4 / W
6
10
11
12 / D / W
6
7
8
10
11
12 / D
10
11 / W / D / W / D / W
8
9 / D / W
9 / D / W
9
10
11
12 / D
10
11 / W
10
11
12 / D
12 / W / D
12 / W / D / W

(c)

Table 3. The tables show the results of the pairwise comparisons for nematode densities. Densities of nematode communities in the Sai Gon River (D: dry season and W: wet season).Since each time the interaction was significant comparisons between seasons (significant differences < 0,05 indicated with *) and stations are shown(stations number that differ from the stations as indicated in the first row are listed).

1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12
* / * / * / * / *
D
3
4
5
6
7
11
12 / W
2
3
4
5
7
11 / D
4 / W
9 / D
4
5
6
8
9
10 / W
9 / D
5
6
7
8
9
10
11
12 / W
9 / D
6
7
8
9
11
12 / W
9 / D
6
7
8
9
11
12 / W / D
8
9
10 / W
9 / D
11
12 / W / D
11
12 / W
11 / D
11
12 / W / D / W / D / W

Table 4.The tables show the results of the pairwise comparisons for each of the genera richness (S)(a) and Shannon index (H’)(b) of nematode communities in dry season (D) and wet season (W). Since each time the interaction was significant comparisons are shown between seasons (significant differences < 0,05 indicated with (*) and stations (stations number that differ from the stations as indicated in the first row are listed).

1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12
* / * / * / *
D
4
7
10 / W
6 / D
4
7
11 / W / D / W / D
5
6
8
9
10 / W / D
7
8
11
12 / W / D
7 / W
9 / D
8
9
10 / W / D
9
10 / W / D
11
12 / W / D
11
12 / W / D / W / D / W

(a)

1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12
* / * / *
D
2
3
5
7
8
10
12 / W
2
6 / D
4
5
6
7
8
12 / W
8
9 / D
4
6
7
8
12 / W / D
5
7
8
10 / W
9 / D
7
8
10
12 / W
8 / D
10 / W
8
9
12 / D
9
10 / W / D
9
10 / W / D
12 / W / D
12 / W / D / W / D / W

(b)

  1. Results of PERMDISP analysis

PERMDISP for standardization of dry season

PERMDISP

Distance-based test for homogeneity of multivariate dispersions

Resemblance worksheet

Name: Resem1

Data type: Similarity

Selection: All

Standardise Samples by Total

Resemblance: S17 Bray Curtis similarity

Group factor: Station

Number of permutations: 9999

Number of groups: 12

Number of samples: 36

DEVIATIONS FROM CENTROID

F: 8,2093 df1: 11 df2: 24

P(perm): 0,0365

MEANS AND STANDARD ERRORS

GroupSizeAverage SE

1 3 11,183 2,0431

2 3 17,247 1,9113

3 3 26,014 3,6448

4 3 46,053 7,2364

5 3 18,858 3,9942

6 3 15,954 2,0398

7 3 10,126 2,5054

8 3 10,7210,61401

9 3 16,461 1,513

10 3 16,597 0,4913

11 3 20,879 6,2813

12 3 10,791 1,1782

PERMDISP for standardization of wet season

PERMDISP

Distance-based test for homogeneity of multivariate dispersions

Resemblance worksheet

Name: Resem3

Data type: Similarity

Selection: All

Standardise Samples by Total

Resemblance: S17 Bray Curtis similarity

Group factor: Station

Number of permutations: 9999

Number of groups: 12

Number of samples: 36

DEVIATIONS FROM CENTROID

F: 4,3298 df1: 11 df2: 24

P(perm): 0,0983

MEANS AND STANDARD ERRORS

GroupSizeAverage SE

1 3 13,7271,8047

2 3 23,4573,7193

3 3 28,9624,2447

4 3 22,1722,0696

5 3 25,9472,0206

6 3 18,7652,3896

7 3 19,8981,5536

8 3 13,1742,8849

9 3 13,792,6715

10 3 33,8165,6544

11 3 27,835,4701

12 3 13,4782,3826