Review Assessment: Unit 01 Study questions
User / Lisa LaverySubmitted / 10/4/09 6:23 PM
Name / Unit 01 Study questions
Status / Completed
Score / 6 out of 15 points
Instructions
Question 1 / 0 out of 1 points
/ Messenger RNA is different from other types of RNA because messenger RNA has:
Selected Answer: / introns and exons
Question 2 / 1 out of 1 points
/ Which of the following is the correct order of the steps that occur in a standard PCR cycle?
Selected Answer: / Denaturation, annealing, and extension
Question 3 / 0 out of 1 points
/ Which of the following methods can be used to screen for the presence of unknown mutations?
Selected Answer: / Multiplex inversion probe assay
Question 4 / 0 out of 1 points
/ When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and the absorptivity constant, which is:
Selected Answer: / 30 μg/mL
Question 5 / 1 out of 1 points
/ In the lactose operon, which of the following configurations would result in gene expression?
Selected Answer: / Promoter +, Operator +, Repressor -, no inducer present
Question 6 / 0 out of 1 points
/ Patient DNA has been mixed in separate wells with a probe that detects the G1691A mutation associated with factor V Leiden and a probe that detects the normal sequence. Cleavase is added to all wells, and the fluorescence is measured in each well. Fluorescence was detected in the well that included the normal probe, and no fluorescence was detected in the well containing the mutant probe. Which of the following is the correct interpretation of these results?
Selected Answer: / The results are not conclusive either way and should be confirmed using a different method.
Question 7 / 1 out of 1 points
/ The process of separating the two DNA strands into two single strands is called:
Selected Answer: / denaturation
Question 8 / 0 out of 1 points
/ The codons that do not code for an amino acid and terminate protein synthesis are what kind of codon?
Selected Answer: / Anticodon
Question 9 / 1 out of 1 points
/ SyBr green fluorescence is detectable at which stage of the PCR amplification process?
Selected Answer: / Primer extension
Question 10 / 0 out of 1 points
/ Which of the following types of RNA is directly involved in removing introns from RNA in eukaryotes?
Selected Answer: / Transfer
Question 11 / 1 out of 1 points
/ The loop of transfer RNA that interacts with the codon on mRNA in translation is called the:
Selected Answer: / anticodon loop
Question 12 / 0 out of 1 points
/ The melting temperature of nucleic acid is the temperature when:
Selected Answer: / all of the double-stranded DNA is single-stranded
Question 13 / 0 out of 1 points
/ The term used to describe the arrangement of the individual strands in the double-stranded DNA molecule is:
Selected Answer: / parallel
Question 14 / 1 out of 1 points
/ A protein that has lost its tertiary structure is called:
Selected Answer: / denatured
Question 15 / 0 out of 1 points
/ A chromosome that has the centromere in the center of the chromosome is called:
Selected Answer: / telocentric
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User / Lisa LaverySubmitted / 10/5/09 1:50 PM
Name / Unit 01 Study questions
Status / Completed
Score / 8 out of 15 points
Instructions
Question 1 / 1 out of 1 points
/ A reference segment of DNA has the following sequence: GCTCACCATGG. The fourth nucleotide is changed to an A in a particular genetic disease. How would this mutation be denoted?
Selected Answer: / 4C>A
Question 2 / 0 out of 1 points
/ A technologist performs fluorescence in situ hybridization (FISH) on interphase chromosomes using a probe to chromosome 21. In all cells examined, two signals were seen. These results are interpreted as:
Selected Answer: / Abnormal; the patient has a Philadelphia chromosome
Question 3 / 0 out of 1 points
/ The set of nucleotides in mRNA that encode for an amino acid is called a(n):
Selected Answer: / codon
Question 4 / 1 out of 1 points
/ Which of the following PCR modifications was developed to allow for the amplification of an RNA template?
Selected Answer: / Reverse transcriptase
Question 5 / 0 out of 1 points
/ Human chromosomes are described as which of the following with regard to the location of the centromere?
Selected Answer: / Genocentric
Question 6 / 0 out of 1 points
/ Which of the following conditions are the least stringent?
Selected Answer: / 40% formamide, 0.2X SSC, 60oC
Question 7 / 1 out of 1 points
/ When gene expression is regulated by mechanisms other than the interaction of cis elements and trans factors, the regulation is called:
Selected Answer: / epigenetics
Question 8 / 1 out of 1 points
/ When chromosomes are stained with Giemsa, the resulting bands are called:
Selected Answer: / G bands
Question 9 / 1 out of 1 points
/ A band that is present in both the hydrazine and hydrazine plus salt-treated sample lanes is read as which of the following nucleotides?
Selected Answer: / T
Question 10 / 0 out of 1 points
/ Which DNA sequence is homologous to 5′GATTCTCAAAGGACT3′?
Selected Answer: / 5′GATTCTCAAAGGACT3′
Question 11 / 1 out of 1 points
/ The fundamental physical and functional unit of inheritance that encodes a functional product is a:
Selected Answer: / gene
Question 12 / 0 out of 1 points
/ Which component of RNA polymerase is responsible for initiating transcription at the right site?
Selected Answer: / β′
Question 13 / 0 out of 1 points
/ A very limited amount of nucleic acid, 500-1500 bp in size, needs to be analyzed in a short time (same day) with the results available immediately. Which of the following electrophoresis procedures will satisfy those conditions?
Selected Answer: / Pulse–field gel electrophoresis
Question 14 / 1 out of 1 points
/ Which of the following types of chromatin are open and actively involved in gene expression (transcription)?
Selected Answer: / Euchromatin
Question 15 / 1 out of 1 points
/ The entire human genome can be analyzed at one time by which of the following methods?
Selected Answer: / Microarray
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User / Lisa LaverySubmitted / 10/5/09 3:08 PM
Name / Unit 01 Study questions
Status / Completed
Score / 7 out of 15 points
Instructions
Question 1 / 1 out of 1 points
/ Which of the following is the first step in DNA isolation from cells in a clinical sample?
Selected Answer: / Lysis of cells
Question 2 / 0 out of 1 points
/ A portion of a sequencing gel is described as follows: the first band at the bottom of the gel was in the ddATP lane; the next band going up the gel was in the ddTTP lane, then the ddCTP lane, the ddGTP lane, the ddATP lane, the ddTTP lane, the ddCTP lane, the ddCTP lane, the ddATP lane, and the ddGTP lane. Which of the following is the correct sequence as determined on this gel?
Selected Answer: / 5′GACCTAGCTA3′
Question 3 / 0 out of 1 points
/ A molecular biologist needs to separate and analyze a mixture of nucleic acids that are about 100,000 bp in size. Which of the following methods will yield the best results?
Selected Answer: / Capillary electrophoresis
Question 4 / 1 out of 1 points
/ A reference segment of DNA has the following sequence: GCTCACCATGG. The fourth nucleotide is changed to an A in a particular genetic disease. How would this mutation be denoted?
Selected Answer: / 4C>A
Question 5 / 1 out of 1 points
/ All but two amino acids are encoded by more than one codon, thus making the genetic code:
Selected Answer: / redundant
Question 6 / 0 out of 1 points
/ A band that is present in only the formic acid–treated sample lanes is read as which of the following nucleotides?
Selected Answer: / G
Question 7 / 0 out of 1 points
/ DNA replication requires the presence of:
Selected Answer: / RNA polymerase
Question 8 / 0 out of 1 points
/ RNA is degraded by:
Selected Answer: / polymerases
Question 9 / 1 out of 1 points
/ In order to monitor the progress of electrophoresis, which of the following is added to a sample that does not associate with DNA and runs ahead of the smallest fragments in the sample?
Selected Answer: / Bromophenol blue
Question 10 / 0 out of 1 points
/ What is the yield of RNA from the sample in which 1:10 dilution had an absorbance reading of 0.675 at 260nm when the sample has a volume of 0.4 mL?
Selected Answer: / 13.5 μg
Question 11 / 0 out of 1 points
/ The process of separating the two DNA strands into two single strands is called:
Selected Answer: / depolymerization
Question 12 / 0 out of 1 points
/ Which component of RNA polymerase is responsible for initiating transcription at the right site?
Selected Answer: / β
Question 13 / 1 out of 1 points
/ In a double-stranded DNA molecule, base-pairing between strands occurs between:
Selected Answer: / a purine and a pyrimidine
Question 14 / 1 out of 1 points
/ When gene expression is regulated by mechanisms other than the interaction of cis elements and trans factors, the regulation is called:
Selected Answer: / epigenetics
Question 15 / 1 out of 1 points
/ When measuring the concentration of RNA by spectrophotometry at 260 nm, the absorbance reading is multiplied by the dilution and the absorptivity constant, which is:
Selected Answer: / 40 μg/mL
2009FA BHS403001 MOLECULAR DIAGNOSTICS (2009FABHS403001) > ASSESSMENTS > REVIEW ASSESSMENT: UNIT 01 STUDY QUESTIONS
Review Assessment: Unit 01 Study questions
User / Lisa LaverySubmitted / 10/5/09 3:28 PM
Name / Unit 01 Study questions
Status / Completed
Score / 6 out of 15 points
Instructions
Question 1 / 1 out of 1 points
/ Which of the following is the first step in DNA isolation from cells in a clinical sample?
Selected Answer: / Lysis of cells
Question 2 / 1 out of 1 points
/ Which of the following is the correct order of steps when performing a Southern blot after isolation of the DNA?
Selected Answer: / Restriction enzyme digestion, electrophoresis, denaturation, probe hybridization
Question 3 / 0 out of 1 points
/ One DNA strand binds to the other strand through:
Selected Answer: / phosphodiester bonds
Question 4 / 1 out of 1 points
/ The large ribosome subunit in prokaryotes consists of ribosomal proteins and:
Selected Answer: / 23S rRNA and 5S rRNA
Question 5 / 0 out of 1 points
/ Colcemid is used in the procedure of preparing a chromosome spread for karyotype analysis to do which of the following?
Selected Answer: / Fix cells to the slide prior to staining
Question 6 / 1 out of 1 points
/ A Sanger sequencing procedure has been performed on a 300-bp piece of DNA. When the fragments were run on the gel, there were bands distributed through the lanes corresponding to ddTTP, ddCTP, and ddATP. In the ddGTP lane, there was one large band at the bottom of the gel. What is the interpretation of these results?
Selected Answer: / No dGTP was added to the reaction mix.
Question 7 / 0 out of 1 points
/ A DNA change that affects the structure of entire chromosomes is which type of mutation?
Selected Answer: / Nucleotide
Question 8 / 0 out of 1 points
/ In inorganic DNA isolation or “salting out” procedures, in the presence of low pH and high salt concentrations, which intracellular component precipitates out of solution?
Selected Answer: / DNA
Question 9 / 1 out of 1 points
/ A portion of a sequencing gel is described as follows: the first band at the bottom of the gel was in the ddATP lane; the next band going up the gel was in the ddTTP lane, then the ddCTP lane, the ddGTP lane, the ddATP lane, the ddTTP lane, the ddCTP lane, the ddCTP lane, the ddATP lane, and the ddGTP lane. Which of the following is the correct sequence as determined on this gel?
Selected Answer: / 5′ATCGATCCAG3′
Question 10 / 0 out of 1 points
/ Isolated DNA has an absorbance at 260 nm of 0.268 and an absorbance at 280 nm of 0.191. What is the 260 nm/280 nm ratio?
Selected Answer: / 1.6
Question 11 / 0 out of 1 points
/ The movement of DNA from one bacterium to another through the activity of bacteriophages is called:
Selected Answer: / conjugation
Question 12 / 1 out of 1 points
/ Which of the following materials is most commonly used to bind nucleic acids in blotting procedures?
Selected Answer: / Nitrocellulose
Question 13 / 0 out of 1 points
/ A melt curve analysis was performed to genotype hepatitis C virus (HCV), which has been identified in a patient. When the derivative of fluorescence was plotted versus the temperature for the patient's isolate as well as for a known genotype of HCV, the two sequences had identical peaks and thus the same Tms. Which of the following is the correct interpretation of these results?
Selected Answer: / The patient's HCV genotype is different from the known genotype.
Question 14 / 0 out of 1 points
/ Transcription-mediated amplification obtains:
Selected Answer: / DNA from RNA and RNA from DNA
Question 15 / 0 out of 1 points
/ In the Sanger sequencing method, which of the following when incorporated into the growing strand are used to determine the identity of the base at a particular position in a piece of DNA?
Selected Answer: / Ribonucleotides