Supplementary Materials

Association between serum uric acid and the metabolic syndrome among a middle- and old-age Chinese population

Journal name: European Journal of Epidemiology

Xiayun Dai1, Jing Yuan1,Ping Yao1,Binyao Yang1,Lixuan Gui1, Xiaomin Zhang1; Huan Guo1, Youjie Wang1,WeihongChen1, ShengWei1, Xiaoping Miao1, Xiulou Li2,Xinwen Min2,Handong Yang2, Weimin Fang1, Yuan Liang1,Frank B Hu3,Tangchun Wu1,MeianHe1

(1) Institute of Occupational Medicine and the Ministry of Education Key Lab of Environment and Health, School of Public Health, Huazhong University of Science and Technology, Wuhan, China.

(2) Dongfeng Central Hospital, Dongfeng Motor Corporation and Hubei University of Medicine, Shiyan, Hubei, China.

(3) Department of Nutrition and Epidemiology, Harvard School of Public Health,Boston, Mass, USA.

Corresponding authors:Meian He

MOE Key Lab of Environment andHealth, School of Public Health, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Rd, Wuhan, Hubei, 430030, China.

E-mail:

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SupplementaryTable 1.Adjusted Odds Ratios and 95% Confidence Intervals for Metabolic Syndrome (defined by modified NCEP criteria) by serum uric quintiles among 23,345 participants

Quintiles of serum uric acid, umol/L
Variables / Q1 / Q2 / Q3 / Q4 / Q5 / P trend
Sample size / 4601 / 4648 / 4718 / 4660 / 4718
Total
Univariate model / 1.00 / 1.38(1.26-1.52) / 1.87(1.71-2.04) / 2.46(2.25-2.68) / 4.39(4.02-4.80) / <0.0001
Age-and sex-adjusted / 1.00 / 1.38(1.25-1.51) / 1.83(1.62-2.01) / 2.39(2.16-2.59) / 4.13(3.77-4.52) / <0.0001
Multivariate model 1a / 1.00 / 1.37(1.25-1.51) / 1.84(1.68-2.02) / 2.36(2.16-2.59) / 4.12 (3.76-4.51) / <0.0001
Multivariate model 2b / 1.00 / 1.36(1.23-1.50) / 1.76(1.60-1.94) / 2.16(1.96-2.38) / 3.37(3.06-3.72) / <0.0001
Men
Univariate model / 1.00 / 1.20(1.04-1.39) / 1.62(1.41-1.86) / 2.01(1.75-2.31) / 3.47(3.03-3.97) / <0.0001
Age-adjusted / 1.00 / 1.20(1.04-1.39) / 1.61(1.40-1.85) / 1.99(1.74-2.29) / 3.43(2.99-3.92) / <0.0001
Multivariate model 1a / 1.00 / 1.20(1.04-1.39) / 1.61(1.40-1.89) / 2.00(1.74-2.29) / 3.42(2.99-3.92) / <0.0001
Multivariate model 2b / 1.00 / 1.16(1.00-1.35) / 1.53(1.32-1.77) / 1.85(1.60-2.14) / 2.74(2.37-3.16) / <0.0001
Women
Univariate model / 1.00 / 1.53(1.36-1.73) / 2.08(1.85-2.34) / 2.89(2.57-3.25) / 5.50(4.87-6.21) / <0.0001
Age- adjusted / 1.00 / 1.50(1.33-1.70) / 2.00(1.77-2.25) / 2.67(2.36-3.01) / 4.70(4.15-5.31) / <0.0001
Multivariate model 1a / 1.00 / 1.50(1.33-1.70) / 2.01(1.78-2.26) / 2.65(2.35-2.99) / 4.68(4.13-5.30) / <0.0001
Multivariate model 2b / 1.00 / 1.52(1.33-1.73) / 1.95(1.71-2.22) / 2.39(2.10-2.72) / 3.93(3.44-4.48) / <0.0001

The quintiles of serum uric acid concentration were calculated by gender respectively. In male, the cutoff of serum uric acid concentration is <261, 261~300, 301~338, 339~389, and ≥390 umol/L respectively; in female, the concentration of serum uric acid is <207, 207~240, 241~274, 275~318, and ≥319 umol/L respectively.

a, adjusted for the age (continuous), gender (male, female) plus smoking (never smoking, quit smoking, smoking), drinking (never drinking, quit drinking, drinking), physical activity (yes/no), and concentrations of creatinine (continuous); In analysis of men and women, adjusted for age (continuous), smoking (never smoking, quit smoking, smoking), drinking (never drinking, quit drinking, drinking), physical activity (yes/no), and concentrations of creatinine (continuous).

b,adjusted for the same set of variables in model 1 plus past history(yes/no) of coronary heart disease, diabetes and hypertension,and medication history(yes/no).

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SupplementaryTable 2. Associations between the uric acid associated SNPs withMetSrisk

Gene / SNPs / Genotypes / MetS / Controls / Crude OR (95% CI) / P value / Adjusted OR / P value
(95% CI)a
SLC2A9 / rs11722228 / CC / 1068(46.1) / 2612(47.7) / 1.00 / 1.00
CT / 1001(43.2) / 2316(42.3) / 1.06(0.95-1.17) / 0.29 / 1.06(0.95-1.17) / 0.32
TT / 246(10.6) / 548(10.0) / 1.10(0.93-1.30) / 0.27 / 1.08(0.91-1.28) / 0.36
ABCG2 / rs2231142 / GG / 1126(48.4) / 2609(47.4) / 1.00 / 1.00
GT / 959(41.2) / 2422(44.0) / 0.92(0.83-1.02) / 0.10 / 0.92(0.83-1.02) / 0.10
TT / 240(10.3) / 471(8.6) / 1.18(1.00-1.40) / 0.06 / 1.19(1.00-1.42) / 0.05

a, adjusted for the age (continuous), gender (male, female) plus smoking (never smoking, quit smoking, smoking), drinking (never

drinking, quit drinking, drinking), physical activity (yes/no), and concentrations of creatinine (continuous).

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Supplementary Table 3.Associations between the genetic risk score and the MetSrisk

GRS / MetS,
n (%) / Controls, n (%) / Crude OR
(95% CI) / P / Adjusted OR
(95% CI)a / P
0 / 534 (23.1) / 1230 (22.5) / 1.00 / 1.00
1 / 893(38.6) / 2255 (41.2) / 0.91(0.80-1.04) / 0.16 / 0.90(0.79-1.03) / 0.12
2 / 663 (28.6) / 1506(27.5) / 1.01(0.89-1.16) / 0.84 / 1.00(0.87-1.15) / 0.99
3 / 194(8.4) / 449 (8.2) / 1.00(0.82-1.21) / 0.96 / 0.99(0.81-1.21) / 0.93
4 / 31(1.3) / 36(0.7) / 1.98(1.21-3.24) / 0.006 / 1.99(1.22-3.27) / 0.006
P trend / 0.20 / 0.24

a, adjusted for the age (continuous), gender (male, female) plus smoking (never smoking, quit

smoking, smoking), drinking (never drinking, quit drinking, drinking), physical activity (yes/no), and concentrations of creatinine (continuous).

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Supplementary Table 4.General characteristics of individuals by uric acid associated genetic risk score

Variables / Genetic risk score / P
0 / 1 / 2 / 3 / 4
Age, y / 63.4(7.2) / 63.4(7.5) / 63.1(7.3) / 63.2(7.3) / 63.4(7.5) / 0.58
Women,% / 55.8 / 57.3 / 58.8 / 56.3 / 53.7 / 0.36
Smoking(smoking/quit/never) ,% / 17.5/9.9/72.7 / 18.0/10.4/71.6 / 15.7/9.6/74.8 / 18.5/9.3/72.2 / 14.9/11.9/73.1 / 0.41
Drinking(drinking/quit/never) ,% / 21.5/4.4/74.1 / 20.1/4.5/75.4 / 19.0/4.5/76.5 / 19.4/4.7/75.9 / 22.4/7.5/70.1 / 0.68
Physical activity (no/yes),% / 11.7/88.3 / 10.6/89.4 / 11.0/89.0 / 12.6/87.4 / 7.5/92.5 / 0.44
Creatinine levels, umol/L / 79.5(25.7) / 79.3(28.5) / 78.8(25.7) / 81.1(30.4) / 81.6(16.8) / 0.42

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