Beta Globin Genotyping HbS/HbC

Homo sapiens genomic beta globin region (HBB@) on chromosome 11

Accession # NG-000007

Tm

BGN-NI5’F–N-tgc acc tga ctc ctGA-P58.3

16 mer

HbS/HbC probe5’F-ctg act cct GTg gag aag tct g-P63.6

22 mer

PCO3 primer5’ aca caa ctg tgt tca cta gc61.9

20 mer

PCO4 primer5’ caa ctt cat cca cgt tca cc63.0

20 mer

BGN2 probe5’[cct gac tcc tGA gga g]-N-F55.9

16 mer

SNP ctgactcctGAggagaagtctg



AT

HbC Hb S

62041 gagccacacc ctagggttgg ccaatctact cccaggagca gggagggcag gagccagggc

62101 tgggcataaa agtcagggca gagccatcta ttgcttacat ttgcttctga cacaactgtg

62161 ttcactagca acctcaaaca gacaccatgg tgca[cctgac tcctGAggag] aagtctgccg

62221 ttactgccct gtggggcaag gtgaacgtgg atgaagttgg tggtgaggcc ctgggcaggt

62281 tggtatcaag gttacaagac aggtttaagg agaccaatag aaactgggca tgtggagaca

Cystic Fibrosis Genotyping: F508del

Human cystic fibrosis transmembrane conductance regulator

(CFTR) gene, exon 10.

Accession # M55115

Tm

CFCAMPR15’ gga ggc aag tga atc ctg ag64.0

20 mer

CFCAMPR25’ cct ctt cta gtt ggc atg ct63.4

20 mer

CFpb5’ F-ata gga aac acc aaa gat gat att ttc]-P59.8

27 mer

CF NI5’ F-N-gaa aca ccaaagatg ata ttt tct tt-P58.7

26 mer

Deletiongaa aca cca aag atg ata ttt



181 gaatcaaatg agttaataga atctttacaa ataagaatat acacttctgc ttaggatgat

241 aattggaggc aagtgaatcc tgagcgtgat ttgataatga cctaataatg atgggtttta

301 tttccagact tcacttctaa tgatgattat gggagaactg gagccttcag agggtaaaat

361 taagcacagt ggaagaattt cattctgttc tcagttttcc tggattatgc ctggcaccat

421 taaa[gaaaat atcatctttg gtgtttccta tgatgaatat agatacagaa gcgtcatcaa

481 agcatgccaa ctagaagagg taagaaacta tgtgaaaact ttttgattat gcatatgaac

541 ccttcacact acccaaatta tatatttggc tccatattca atcggttagt ctacatatat

Factor V Leiden Genotyping: G1691A

LOCUS HUMF510

Human coagulation factor V gene, exon 10.

ACCESSION L32764 J05368

Tm

Ber12 5’ GAC ATC GCC TCT GGG CTA65.1

18 mer

FacV35’ AGA ATA AAT GTT ATC ACA CTG GTG CTA A64.2

28 mer

5FVN25’ F-N-ATT CCT CGC CTG TCC-P56.6

15 mer

3FVN25’ ATT CCT CGC CTG TCC-N-F56.5

15 mer

F5pb5’ F-CTG TAT TCC TCG CCT GTC]-P58.8

18 mer

FV-NI5’ F-N-TTA GAA GTA GTC CTA TTA GCC-P55.2

21 mer

gacaggcGaggaata



A

1 ttatttatta tcatgaaata actttgcaaa tgaaaacaat tttgaatata ttttctttca

61 ggcaggaaca acaccatgat cagagcagtt caaccagggg aaacctatac ttataagtgg

121 aacatcttag agtttgatga acccacagaa aatgatgccc agtgcttaac aagaccatac

181 tacagtgacg tggacatcat gagagacatc gcctctgggc taataggact acttctaatc

241 tgtaagagca gatccctg[ga caggcgagga atacaggtat tttgtccttg aagtaacctt

301 tcagaaattc tgagaattt

Human DNA sequence from PAC 86F14 on chromosome 1q23-1q24.

Contains coagulation Factor V, ESTs and STS.

ACCESSION Z99572

62701 acactctaga cttgccttcg gcagtgatgg tactgataaa aatagacaag acaaaaaaaa

62761 aaaaagaata aatgttatca cactggtgct aaaaaggact acttgacaat tactgttctc

62821 ttgaaggaaa tgccccatta tttagccagg agacctaaca tgttctagcc agaagaaatt

62881 ctcagaattt ctgaaaggtt acttcaagga caaaatacct gtattccttg cctgtc]cagg

62941 gatctgctct tacagattag aagtagtcct attagcccag aggcgatgtc tctcatgatg

63001 tccacgtcac tgtagtatgg tcttgttaag cactgggcat catttt

Hemochromotosis Genotyping: H63D

Homo sapiens HFE gene - hemochromatosis

Accession # Z92910

Tm

H63D F5’ cac atg gtt aag gcc tgt tg63.0

20 mer

H63D R5’ gat ccc acc ctt tca gac tc63.4

20 mer

H63Dpb5’ F-cac acg gcg act ctc atc atc ata gaa c-P68.6

28 mer

H63D-NI5’ F-N-aca cga aca gct ggt c-P58.2

16 mer

H63D-N25’[gtt cgt gtt cta tga tca tg]-5N-F55.8

20 mer

SNPgttctatgatCatgagagtcgc



G

4441 tgagggggtg gcagccacgt gtggcagaga aaagcacaca aggaaagagc acccaggact

4501 gtcatatgga agaaagacag gactgcaact cacccttcac aaaatgagga ccagacacag

4561 ctgatggtat gagttgatgc aggtgtgtgg agcctcaaca tcctgctccc ctcctactac

4621 acatggttaaggcctgttgc tctgtctcca ggttcacact ctctgcacta cctcttcatg

4681 ggtgcctcag agcaggacct tggtctttcc ttgtttgaag ctttgggcta cgtggatgac

4741 cagct[gttcg tgttctatga tcatg]agagt cgccgtgtgg agccccgaac tccatgggtt

4801 tccagtagaa tttcaagcca gatgtggctg cagctgagtc agagtctgaa agggtgggat

4861 cacatgttca ctgttgactt ctggactatt atggaaaatc acaaccacag caagggtatg

4921 tggagagggg gcctcacctt cctgaggttg tcagagcttt tcatcttttc atgcatcttg

Hemochromotosis Genotyping: C282Y

Homo sapiens HFE gene - hemochromatosis

Accession # Z92910

Tm

HLA-H15’ tgg caa ggg taa aca gat cc63.7

20 mer

HLA-H25’ ctc agg cac tcc tct caa cc65.9

20 mer

C282Ypb5’ F-cac ctg gca cgt ata tct ctg-P63.8

21 mer

C282Y NI5’ F-N-tat ctc tgc tct tcc cca g-P59.4

19 mer

3C282Y N25’ [gag ata tac gtg cca gg]-N-F55.2

17 mer

SNPcagagatatacgtGccaggtg



A

6361 gtctctcctg tagcttgttt ttttctgaaa agggtatttc cttcctccaa cctatagaag

6421 gaagtgaaag ttccagtctt cctggcaagg gtaaacagat cccctctcct catccttcct

6481 ctttcctgtc aagtgcctcc tttggtgaag gtgacacatc atgtgacctc ttcagtgacc

6541 actctacggt gtcgggcctt gaactactac ccccagaaca tcaccatgaa gtggctgaag

6601 gataagcagc caatggatgc caaggagttc gaacctaaag acgtattgcc caatggggat

6661 gggacctacc agggctggat aaccttggct gtaccccctg gggaagagca[gagatatacg

6721 tgccagg]tgg agcacccagg cctggatcag cccctcattg tgatctgggg tatgtgactg

6781 atgagagcca ggagctgaga aaatctattg ggggttgaga ggagtgcctg aggaggtaat

6841 tatggcagtg agatgaggat ctgctctttg ttaggggatg ggctgagggt ggcaatcaaa

MCAD Genotyping: A985G

Homo sapiens acyl-Coenzyme A dehydrogenase,

C-4 to C-12 straight chain (ACADM), mRNA

Accession # XM_038821

Tm

MCAD F5’ cca agc aat atc att tat gct ggc64.4

24 mer

MCAD R5’ tgt att aaa tcc att gcc tcc aag tat64.0

27 mer

MCADprb-S5’ att cta gct agt tca act tcc att gc-F63.1

26 mer

MCADprb-L5’ att ctagct agt tca act tcc att gcc att tca-F 68.2

33 mer

MCAD ???5’ F-gca atg aaa gtt gaa cta gct aga-P60.9

24 mer

5MCAD NI5’ F-N-gca atg aaa gtt gaa cta gc-P57.2

20 mer

aaatggcaatgAaagttgaactag



G

2221 tgataaaacc agacctgtag tagctgctgg tgctgttgga ttagcacaaa gagctttgga

2281 tgaagctacc aagtatgccc tggaaaggaa aactttcgga aagctacttg tagagcacca

2341 agcaatatca tttatgctgg ctgaaatggc aatgaaagtt gaactagcta gaatgagtta

2401 ccagagagca gcttgggagg ttgattctgg tcgtcgaaat acctattatg cttctattgc

2461 aaaggcattt gctggagata ttgcaaatca gttagctact gatgctgtgc agatacttgg

2521 aggcaatgga tttaatacag aatatcctgt agaaaaacta atgagggatg ccaaaatcta

2581 tcagatttat gaaggtactt cacaaattca aagacttatt gtagcccgtg aacacattga

MTHFR Genotyping C677T

5,10-MethyleneTetraHydroFolate Reductase

Homo sapiens chromosome 1 clone RP11-196P5 map 1, 18 unordered pieces.

Accession # AC025001

Tm

MTHFR S5’gct ttg agg ctg acc tga63.5

18 mer

MTHFR AS5’acg gtg cgg tga gag tg66.1

17 mer

MTHFR AS25’acg gtg cgg tga gag t64.8

16 mer

MTHFRpb5’F-tgc gtg atg atg aaa tcggct cc –P67.4

23 mer

3MTH-N25’ctgcgg gagccg a–N-F54.9

13 mer

SNPcgggagCcgatttc



T

87661 atccctcgcc ttgaacaggt ggaggccagc ctctcctgac tgtcatccct attggcaggt

87721 taccccaaag gccaccccga agcagggagc tttgaggctg acctgaagca cttgaaggag

87781 aaggtgtctgcgggagccgatttcatcatc acgcagcttt tctttgaggc tgacacattc

87841 ttccgctttg tgaaggcatg caccgacatg ggcatcactt gccccatcgt ccccgggatc

87901 tttcccatcc aggtgagggg cccaggagag cccataagct ccctccaccc cactctcacc

87961 gcaccgtcct cgcacaggct gggggctctg ggtggagtgc tgagttcgct gagttcttcc

88021 cagatctcct ctcaggtcca gaacttgcac agcgttgctt ggccacccca ttttggttac

Prothrombin Genotyping: G20210A

Human prothrombin (F2) gene, complete cds, and Alu and KpnI repeats.

Accession # M17262 M33691

Tm

PT-S5’ gtc att gat cag ttt gga gag tag g64.3

25 mer

PT-AS5’ gag ctg ccc atg aat agc a63.9

19 mer

F2pb5’ F-tct cag caa gcc tca atg ct-P63.0

20 mer

5PT-NI5’ F-N-gag gct cgc tga ga-P55.3

14 mer

actctcagcGagcctcaa



A

26521 gaaacctcat ctttcttctt cagagcccct ttaacaaccg ctggtatcaa atgggcatcg

26581 tctcatgggg tgaaggctgt gaccgggatg ggaaatatgg cttctacaca catgtgttcc

26641 gcctgaagaa gtggatacag aaggtcattg atcagtttgg agagtagggg gccactcata

26701 ttctgggctc ctggaaccaa tcccgtgaaa gaattatttt tgtgtttcta aaactatggt

26761 tcccaataaa agtgactctc agcgagcctcaatgctccca gtgctattca tgggcagctc

26821 tctgggctca ggaagagcca gtaatactac tggataaaga agacttaaga atccaccacc

26881 tggtgcacgc tggtagtccg agcactcggg aggctgaggt gggaggat