Beta Globin Genotyping HbS/HbC
Homo sapiens genomic beta globin region (HBB@) on chromosome 11
Accession # NG-000007
Tm
BGN-NI5’F–N-tgc acc tga ctc ctGA-P58.3
16 mer
HbS/HbC probe5’F-ctg act cct GTg gag aag tct g-P63.6
22 mer
PCO3 primer5’ aca caa ctg tgt tca cta gc61.9
20 mer
PCO4 primer5’ caa ctt cat cca cgt tca cc63.0
20 mer
BGN2 probe5’[cct gac tcc tGA gga g]-N-F55.9
16 mer
SNP ctgactcctGAggagaagtctg
AT
HbC Hb S
62041 gagccacacc ctagggttgg ccaatctact cccaggagca gggagggcag gagccagggc
62101 tgggcataaa agtcagggca gagccatcta ttgcttacat ttgcttctga cacaactgtg
62161 ttcactagca acctcaaaca gacaccatgg tgca[cctgac tcctGAggag] aagtctgccg
62221 ttactgccct gtggggcaag gtgaacgtgg atgaagttgg tggtgaggcc ctgggcaggt
62281 tggtatcaag gttacaagac aggtttaagg agaccaatag aaactgggca tgtggagaca
Cystic Fibrosis Genotyping: F508del
Human cystic fibrosis transmembrane conductance regulator
(CFTR) gene, exon 10.
Accession # M55115
Tm
CFCAMPR15’ gga ggc aag tga atc ctg ag64.0
20 mer
CFCAMPR25’ cct ctt cta gtt ggc atg ct63.4
20 mer
CFpb5’ F-ata gga aac acc aaa gat gat att ttc]-P59.8
27 mer
CF NI5’ F-N-gaa aca ccaaagatg ata ttt tct tt-P58.7
26 mer
Deletiongaa aca cca aag atg ata ttt
181 gaatcaaatg agttaataga atctttacaa ataagaatat acacttctgc ttaggatgat
241 aattggaggc aagtgaatcc tgagcgtgat ttgataatga cctaataatg atgggtttta
301 tttccagact tcacttctaa tgatgattat gggagaactg gagccttcag agggtaaaat
361 taagcacagt ggaagaattt cattctgttc tcagttttcc tggattatgc ctggcaccat
421 taaa[gaaaat atcatctttg gtgtttccta tgatgaatat agatacagaa gcgtcatcaa
481 agcatgccaa ctagaagagg taagaaacta tgtgaaaact ttttgattat gcatatgaac
541 ccttcacact acccaaatta tatatttggc tccatattca atcggttagt ctacatatat
Factor V Leiden Genotyping: G1691A
LOCUS HUMF510
Human coagulation factor V gene, exon 10.
ACCESSION L32764 J05368
Tm
Ber12 5’ GAC ATC GCC TCT GGG CTA65.1
18 mer
FacV35’ AGA ATA AAT GTT ATC ACA CTG GTG CTA A64.2
28 mer
5FVN25’ F-N-ATT CCT CGC CTG TCC-P56.6
15 mer
3FVN25’ ATT CCT CGC CTG TCC-N-F56.5
15 mer
F5pb5’ F-CTG TAT TCC TCG CCT GTC]-P58.8
18 mer
FV-NI5’ F-N-TTA GAA GTA GTC CTA TTA GCC-P55.2
21 mer
gacaggcGaggaata
A
1 ttatttatta tcatgaaata actttgcaaa tgaaaacaat tttgaatata ttttctttca
61 ggcaggaaca acaccatgat cagagcagtt caaccagggg aaacctatac ttataagtgg
121 aacatcttag agtttgatga acccacagaa aatgatgccc agtgcttaac aagaccatac
181 tacagtgacg tggacatcat gagagacatc gcctctgggc taataggact acttctaatc
241 tgtaagagca gatccctg[ga caggcgagga atacaggtat tttgtccttg aagtaacctt
301 tcagaaattc tgagaattt
Human DNA sequence from PAC 86F14 on chromosome 1q23-1q24.
Contains coagulation Factor V, ESTs and STS.
ACCESSION Z99572
62701 acactctaga cttgccttcg gcagtgatgg tactgataaa aatagacaag acaaaaaaaa
62761 aaaaagaata aatgttatca cactggtgct aaaaaggact acttgacaat tactgttctc
62821 ttgaaggaaa tgccccatta tttagccagg agacctaaca tgttctagcc agaagaaatt
62881 ctcagaattt ctgaaaggtt acttcaagga caaaatacct gtattccttg cctgtc]cagg
62941 gatctgctct tacagattag aagtagtcct attagcccag aggcgatgtc tctcatgatg
63001 tccacgtcac tgtagtatgg tcttgttaag cactgggcat catttt
Hemochromotosis Genotyping: H63D
Homo sapiens HFE gene - hemochromatosis
Accession # Z92910
Tm
H63D F5’ cac atg gtt aag gcc tgt tg63.0
20 mer
H63D R5’ gat ccc acc ctt tca gac tc63.4
20 mer
H63Dpb5’ F-cac acg gcg act ctc atc atc ata gaa c-P68.6
28 mer
H63D-NI5’ F-N-aca cga aca gct ggt c-P58.2
16 mer
H63D-N25’[gtt cgt gtt cta tga tca tg]-5N-F55.8
20 mer
SNPgttctatgatCatgagagtcgc
G
4441 tgagggggtg gcagccacgt gtggcagaga aaagcacaca aggaaagagc acccaggact
4501 gtcatatgga agaaagacag gactgcaact cacccttcac aaaatgagga ccagacacag
4561 ctgatggtat gagttgatgc aggtgtgtgg agcctcaaca tcctgctccc ctcctactac
4621 acatggttaaggcctgttgc tctgtctcca ggttcacact ctctgcacta cctcttcatg
4681 ggtgcctcag agcaggacct tggtctttcc ttgtttgaag ctttgggcta cgtggatgac
4741 cagct[gttcg tgttctatga tcatg]agagt cgccgtgtgg agccccgaac tccatgggtt
4801 tccagtagaa tttcaagcca gatgtggctg cagctgagtc agagtctgaa agggtgggat
4861 cacatgttca ctgttgactt ctggactatt atggaaaatc acaaccacag caagggtatg
4921 tggagagggg gcctcacctt cctgaggttg tcagagcttt tcatcttttc atgcatcttg
Hemochromotosis Genotyping: C282Y
Homo sapiens HFE gene - hemochromatosis
Accession # Z92910
Tm
HLA-H15’ tgg caa ggg taa aca gat cc63.7
20 mer
HLA-H25’ ctc agg cac tcc tct caa cc65.9
20 mer
C282Ypb5’ F-cac ctg gca cgt ata tct ctg-P63.8
21 mer
C282Y NI5’ F-N-tat ctc tgc tct tcc cca g-P59.4
19 mer
3C282Y N25’ [gag ata tac gtg cca gg]-N-F55.2
17 mer
SNPcagagatatacgtGccaggtg
A
6361 gtctctcctg tagcttgttt ttttctgaaa agggtatttc cttcctccaa cctatagaag
6421 gaagtgaaag ttccagtctt cctggcaagg gtaaacagat cccctctcct catccttcct
6481 ctttcctgtc aagtgcctcc tttggtgaag gtgacacatc atgtgacctc ttcagtgacc
6541 actctacggt gtcgggcctt gaactactac ccccagaaca tcaccatgaa gtggctgaag
6601 gataagcagc caatggatgc caaggagttc gaacctaaag acgtattgcc caatggggat
6661 gggacctacc agggctggat aaccttggct gtaccccctg gggaagagca[gagatatacg
6721 tgccagg]tgg agcacccagg cctggatcag cccctcattg tgatctgggg tatgtgactg
6781 atgagagcca ggagctgaga aaatctattg ggggttgaga ggagtgcctg aggaggtaat
6841 tatggcagtg agatgaggat ctgctctttg ttaggggatg ggctgagggt ggcaatcaaa
MCAD Genotyping: A985G
Homo sapiens acyl-Coenzyme A dehydrogenase,
C-4 to C-12 straight chain (ACADM), mRNA
Accession # XM_038821
Tm
MCAD F5’ cca agc aat atc att tat gct ggc64.4
24 mer
MCAD R5’ tgt att aaa tcc att gcc tcc aag tat64.0
27 mer
MCADprb-S5’ att cta gct agt tca act tcc att gc-F63.1
26 mer
MCADprb-L5’ att ctagct agt tca act tcc att gcc att tca-F 68.2
33 mer
MCAD ???5’ F-gca atg aaa gtt gaa cta gct aga-P60.9
24 mer
5MCAD NI5’ F-N-gca atg aaa gtt gaa cta gc-P57.2
20 mer
aaatggcaatgAaagttgaactag
G
2221 tgataaaacc agacctgtag tagctgctgg tgctgttgga ttagcacaaa gagctttgga
2281 tgaagctacc aagtatgccc tggaaaggaa aactttcgga aagctacttg tagagcacca
2341 agcaatatca tttatgctgg ctgaaatggc aatgaaagtt gaactagcta gaatgagtta
2401 ccagagagca gcttgggagg ttgattctgg tcgtcgaaat acctattatg cttctattgc
2461 aaaggcattt gctggagata ttgcaaatca gttagctact gatgctgtgc agatacttgg
2521 aggcaatgga tttaatacag aatatcctgt agaaaaacta atgagggatg ccaaaatcta
2581 tcagatttat gaaggtactt cacaaattca aagacttatt gtagcccgtg aacacattga
MTHFR Genotyping C677T
5,10-MethyleneTetraHydroFolate Reductase
Homo sapiens chromosome 1 clone RP11-196P5 map 1, 18 unordered pieces.
Accession # AC025001
Tm
MTHFR S5’gct ttg agg ctg acc tga63.5
18 mer
MTHFR AS5’acg gtg cgg tga gag tg66.1
17 mer
MTHFR AS25’acg gtg cgg tga gag t64.8
16 mer
MTHFRpb5’F-tgc gtg atg atg aaa tcggct cc –P67.4
23 mer
3MTH-N25’ctgcgg gagccg a–N-F54.9
13 mer
SNPcgggagCcgatttc
T
87661 atccctcgcc ttgaacaggt ggaggccagc ctctcctgac tgtcatccct attggcaggt
87721 taccccaaag gccaccccga agcagggagc tttgaggctg acctgaagca cttgaaggag
87781 aaggtgtctgcgggagccgatttcatcatc acgcagcttt tctttgaggc tgacacattc
87841 ttccgctttg tgaaggcatg caccgacatg ggcatcactt gccccatcgt ccccgggatc
87901 tttcccatcc aggtgagggg cccaggagag cccataagct ccctccaccc cactctcacc
87961 gcaccgtcct cgcacaggct gggggctctg ggtggagtgc tgagttcgct gagttcttcc
88021 cagatctcct ctcaggtcca gaacttgcac agcgttgctt ggccacccca ttttggttac
Prothrombin Genotyping: G20210A
Human prothrombin (F2) gene, complete cds, and Alu and KpnI repeats.
Accession # M17262 M33691
Tm
PT-S5’ gtc att gat cag ttt gga gag tag g64.3
25 mer
PT-AS5’ gag ctg ccc atg aat agc a63.9
19 mer
F2pb5’ F-tct cag caa gcc tca atg ct-P63.0
20 mer
5PT-NI5’ F-N-gag gct cgc tga ga-P55.3
14 mer
actctcagcGagcctcaa
A
26521 gaaacctcat ctttcttctt cagagcccct ttaacaaccg ctggtatcaa atgggcatcg
26581 tctcatgggg tgaaggctgt gaccgggatg ggaaatatgg cttctacaca catgtgttcc
26641 gcctgaagaa gtggatacag aaggtcattg atcagtttgg agagtagggg gccactcata
26701 ttctgggctc ctggaaccaa tcccgtgaaa gaattatttt tgtgtttcta aaactatggt
26761 tcccaataaa agtgactctc agcgagcctcaatgctccca gtgctattca tgggcagctc
26821 tctgggctca ggaagagcca gtaatactac tggataaaga agacttaaga atccaccacc
26881 tggtgcacgc tggtagtccg agcactcggg aggctgaggt gggaggat