Table 1

Results of the two-way ANOVA for the effects of “station”, “season” and interaction between the two factors on metal concentrations in the sediments

Elements / Variation origin / df / MS / F-ration / p-value
Cd / Station effect / 4 / 0.442 / 3.642 / <0.05
Season effect / 4 / 1.182 / 9.747 / <0.01
Interaction / 16 / 0.376 / 3.099 / <0.01
Error / 50 / 0.121
Pb / Station effect / 4 / 190.352 / 5.665 / <0.01
Season effect / 4 / 416.505 / 12.395 / <0.01
Interaction / 16 / 166.038 / 4.941 / <0.01
Error / 50 / 33.601
Cu / Station effect / 4 / 298.101 / 11.900 / <0.01
Season effect / 4 / 178.551 / 7.128 / <0.01
Interaction / 16 / 408.648 / 16.313 / <0.01
Error / 50 / 25.050
Zn / Station effect / 4 / 2359.500 / 29.765 / <0.01
Season effect / 4 / 4566.141 / 57.602 / <0.01
Interaction / 16 / 1779.001 / 22.442 / <0.01
Error / 50 / 79.271
Mn / Station effect / 4 / 79033.532 / 9.941 / <0.01
Season effect / 4 / 292818.891 / 36.833 / <0.01
Interaction / 16 / 92634.617 / 11.652 / <0.01
Error / 50 / 7949.898
Cr / Station effect / 4 / 405.173 / 5.762 / <0.01
Season effect / 4 / 2118.403 / 30.128 / <0.01
Interaction / 16 / 508.703 / 7.235 / <0.01
Error / 50 / 70.313
Ni / Station effect / 4 / 370.001 / 6.136 / <0.01
Season effect / 4 / 558.854 / 9.268 / <0.01
Interaction / 16 / 280.685 / 4.655 / <0.01
Error / 50 / 60.298

Significant effects were only considered if p<0.05.

Table 2

Results of the two-way ANOVA for the effects of “station”, “season” and interaction between the two factors on metal concentrations in R. philippinarum

Elements / Variation origin / df / MS / F-ration / p-value
Cd / Station effect / 4 / 0.808 / 4.559 / <0.01
Season effect / 4 / 4.579 / 25.854 / <0.01
Interaction / 16 / 0.488 / 2.753 / <0.01
Error / 50 / 0.177
Pb / Station effect / 4 / 72.732 / 253.389 / <0.01
Season effect / 4 / 143.023 / 498.272 / <0.01
Interaction / 16 / 31.499 / 109.740 / <0.01
Error / 50 / 0.287
Cu / Station effect / 4 / 80.047 / 21.252 / <0.01
Season effect / 4 / 212.101 / 56.312 / <0.01
Interaction / 16 / 34.879 / 9.260 / <0.01
Error / 50 / 3.767
Zn / Station effect / 4 / 337.293 / 4.823 / <0.01
Season effect / 4 / 2382.358 / 34.065 / <0.01
Interaction / 16 / 346.522 / 4.955 / <0.01
Error / 50 / 69.936
Mn / Station effect / 4 / 815.717 / 37.161 / <0.01
Season effect / 4 / 3524.111 / 160.544 / <0.01
Interaction / 16 / 277.499 / 12.642 / <0.01
Error / 50 / 21.951
Cr / Station effect / 4 / 235.238 / 8.206 / <0.01
Season effect / 4 / 400.663 / 13.977 / <0.01
Interaction / 16 / 50.175 / 1.750 / >0.05
Error / 50 / 28.665
Ni / Station effect / 4 / 186.581 / 8.906 / <0.01
Season effect / 4 / 1638.568 / 78.209 / <0.01
Interaction / 16 / 176.002 / 8.401 / <0.01
Error / 50 / 20.951

Significant effects were only considered if p<0.05.

Tests of Between-Subjects Effects

Dependent Variable: sediment Cd

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 12.512(b) / 24 / .521 / 4.297 / .000 / .673 / 103.134 / 1.000
Intercept / 108.263 / 1 / 108.263 / 892.424 / .000 / .947 / 892.424 / 1.000
a / 1.767 / 4 / .442 / 3.642 / .011 / .226 / 14.566 / .845
b / 4.730 / 4 / 1.182 / 9.747 / .000 / .438 / 38.987 / .999
a * b / 6.015 / 16 / .376 / 3.099 / .001 / .498 / 49.581 / .993
Error / 6.066 / 50 / .121
Total / 126.841 / 75
Corrected Total / 18.577 / 74

a Computed using alpha = .05

b R Squared = .673 (Adjusted R Squared = .517)

Tests of Between-Subjects Effects

Dependent Variable: sediment Pb

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 5084.038(b) / 24 / 211.835 / 6.304 / .000 / .752 / 151.304 / 1.000
Intercept / 26616.561 / 1 / 26616.561 / 792.126 / .000 / .941 / 792.126 / 1.000
a / 761.410 / 4 / 190.352 / 5.665 / .001 / .312 / 22.660 / .969
b / 1666.018 / 4 / 416.505 / 12.395 / .000 / .498 / 49.582 / 1.000
a * b / 2656.610 / 16 / 166.038 / 4.941 / .000 / .613 / 79.062 / 1.000
Error / 1680.071 / 50 / 33.601
Total / 33380.670 / 75
Corrected Total / 6764.109 / 74

a Computed using alpha = .05

b R Squared = .752 (Adjusted R Squared = .632)

Tests of Between-Subjects Effects

Dependent Variable: sediment Cu

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 8444.980(b) / 24 / 351.874 / 14.047 / .000 / .871 / 337.123 / 1.000
Intercept / 27773.168 / 1 / 27773.168 / 1108.703 / .000 / .957 / 1108.703 / 1.000
a / 1192.403 / 4 / 298.101 / 11.900 / .000 / .488 / 47.601 / 1.000
b / 714.203 / 4 / 178.551 / 7.128 / .000 / .363 / 28.511 / .992
a * b / 6538.374 / 16 / 408.648 / 16.313 / .000 / .839 / 261.012 / 1.000
Error / 1252.507 / 50 / 25.050
Total / 37470.655 / 75
Corrected Total / 9697.487 / 74

a Computed using alpha = .05

b R Squared = .871 (Adjusted R Squared = .809)

Tests of Between-Subjects Effects

Dependent Variable: sediment Zn

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 56166.574(b) / 24 / 2340.274 / 29.522 / .000 / .934 / 708.537 / 1.000
Intercept / 369758.970 / 1 / 369758.970 / 4664.483 / .000 / .989 / 4664.483 / 1.000
a / 9438.000 / 4 / 2359.500 / 29.765 / .000 / .704 / 119.060 / 1.000
b / 18264.563 / 4 / 4566.141 / 57.602 / .000 / .822 / 230.406 / 1.000
a * b / 28464.011 / 16 / 1779.001 / 22.442 / .000 / .878 / 359.072 / 1.000
Error / 3963.558 / 50 / 79.271
Total / 429889.101 / 75
Corrected Total / 60130.131 / 74

a Computed using alpha = .05

b R Squared = .934 (Adjusted R Squared = .902)

Tests of Between-Subjects Effects

Dependent Variable: sediment Mn

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 2969563.557(b) / 24 / 123731.815 / 15.564 / .000 / .882 / 373.535 / 1.000
Intercept / 14447709.733 / 1 / 14447709.733 / 1817.345 / .000 / .973 / 1817.345 / 1.000
a / 316134.130 / 4 / 79033.532 / 9.941 / .000 / .443 / 39.766 / .999
b / 1171275.563 / 4 / 292818.891 / 36.833 / .000 / .747 / 147.332 / 1.000
a * b / 1482153.865 / 16 / 92634.617 / 11.652 / .000 / .789 / 186.437 / 1.000
Error / 397494.895 / 50 / 7949.898
Total / 17814768.185 / 75
Corrected Total / 3367058.452 / 74

a Computed using alpha = .05

b R Squared = .882 (Adjusted R Squared = .825)

Tests of Between-Subjects Effects

Dependent Variable: sediment Cr

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 18233.545(b) / 24 / 759.731 / 10.805 / .000 / .838 / 259.321 / 1.000
Intercept / 103856.310 / 1 / 103856.310 / 1477.066 / .000 / .967 / 1477.066 / 1.000
a / 1620.690 / 4 / 405.173 / 5.762 / .001 / .316 / 23.050 / .971
b / 8473.613 / 4 / 2118.403 / 30.128 / .000 / .707 / 120.513 / 1.000
a * b / 8139.242 / 16 / 508.703 / 7.235 / .000 / .698 / 115.758 / 1.000
Error / 3515.629 / 50 / 70.313
Total / 125605.484 / 75
Corrected Total / 21749.174 / 74

a Computed using alpha = .05

b R Squared = .838 (Adjusted R Squared = .761)

Tests of Between-Subjects Effects

Dependent Variable: sediment Ni

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 8206.383(b) / 24 / 341.933 / 5.671 / .000 / .731 / 136.098 / 1.000
Intercept / 109877.473 / 1 / 109877.473 / 1822.249 / .000 / .973 / 1822.249 / 1.000
a / 1480.003 / 4 / 370.001 / 6.136 / .000 / .329 / 24.545 / .979
b / 2235.417 / 4 / 558.854 / 9.268 / .000 / .426 / 37.073 / .999
a * b / 4490.963 / 16 / 280.685 / 4.655 / .000 / .598 / 74.480 / 1.000
Error / 3014.886 / 50 / 60.298
Total / 121098.742 / 75
Corrected Total / 11221.269 / 74

a Computed using alpha = .05

b R Squared = .731 (Adjusted R Squared = .602)

Tests of Between-Subjects Effects

Dependent Variable: clam Cd

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 29.351(b) / 24 / 1.223 / 6.904 / .000 / .768 / 165.705 / 1.000
Intercept / 142.945 / 1 / 142.945 / 807.026 / .000 / .942 / 807.026 / 1.000
a / 3.230 / 4 / .808 / 4.559 / .003 / .267 / 18.237 / .922
b / 18.318 / 4 / 4.579 / 25.854 / .000 / .674 / 103.416 / 1.000
a * b / 7.803 / 16 / .488 / 2.753 / .003 / .468 / 44.052 / .983
Error / 8.856 / 50 / .177
Total / 181.152 / 75
Corrected Total / 38.207 / 74

a Computed using alpha = .05

b R Squared = .768 (Adjusted R Squared = .657)

Tests of Between-Subjects Effects

Dependent Variable: clam Pb

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 1367.009(b) / 24 / 56.959 / 198.437 / .000 / .990 / 4762.476 / 1.000
Intercept / 1339.264 / 1 / 1339.264 / 4665.817 / .000 / .989 / 4665.817 / 1.000
a / 290.928 / 4 / 72.732 / 253.389 / .000 / .953 / 1013.555 / 1.000
b / 572.090 / 4 / 143.023 / 498.272 / .000 / .976 / 1993.086 / 1.000
a * b / 503.990 / 16 / 31.499 / 109.740 / .000 / .972 / 1755.834 / 1.000
Error / 14.352 / 50 / .287
Total / 2720.625 / 75
Corrected Total / 1381.361 / 74

a Computed using alpha = .05

b R Squared = .990 (Adjusted R Squared = .985)

Tests of Between-Subjects Effects

Dependent Variable: clam Cu

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 1726.655(b) / 24 / 71.944 / 19.101 / .000 / .902 / 458.420 / 1.000
Intercept / 8718.945 / 1 / 8718.945 / 2314.845 / .000 / .979 / 2314.845 / 1.000
a / 320.189 / 4 / 80.047 / 21.252 / .000 / .630 / 85.009 / 1.000
b / 848.403 / 4 / 212.101 / 56.312 / .000 / .818 / 225.248 / 1.000
a * b / 558.063 / 16 / 34.879 / 9.260 / .000 / .748 / 148.164 / 1.000
Error / 188.327 / 50 / 3.767
Total / 10633.926 / 75
Corrected Total / 1914.981 / 74

a Computed using alpha = .05

b R Squared = .902 (Adjusted R Squared = .854)

Tests of Between-Subjects Effects

Dependent Variable: clam Zn

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 16422.961(b) / 24 / 684.290 / 9.784 / .000 / .824 / 234.827 / 1.000
Intercept / 424549.561 / 1 / 424549.561 / 6070.503 / .000 / .992 / 6070.503 / 1.000
a / 1349.172 / 4 / 337.293 / 4.823 / .002 / .278 / 19.291 / .937
b / 9529.433 / 4 / 2382.358 / 34.065 / .000 / .732 / 136.258 / 1.000
a * b / 5544.356 / 16 / 346.522 / 4.955 / .000 / .613 / 79.277 / 1.000
Error / 3496.824 / 50 / 69.936
Total / 444469.345 / 75
Corrected Total / 19919.785 / 74

a Computed using alpha = .05

b R Squared = .824 (Adjusted R Squared = .740)

Tests of Between-Subjects Effects

Dependent Variable: clam Mn

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 21799.298(b) / 24 / 908.304 / 41.379 / .000 / .952 / 993.087 / 1.000
Intercept / 81334.617 / 1 / 81334.617 / 3705.275 / .000 / .987 / 3705.275 / 1.000
a / 3262.867 / 4 / 815.717 / 37.161 / .000 / .748 / 148.643 / 1.000
b / 14096.442 / 4 / 3524.111 / 160.544 / .000 / .928 / 642.177 / 1.000
a * b / 4439.988 / 16 / 277.499 / 12.642 / .000 / .802 / 202.268 / 1.000
Error / 1097.552 / 50 / 21.951
Total / 104231.466 / 75
Corrected Total / 22896.850 / 74

a Computed using alpha = .05

b R Squared = .952 (Adjusted R Squared = .929)

Tests of Between-Subjects Effects

Dependent Variable: clam Cr

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 3346.399(b) / 24 / 139.433 / 4.864 / .000 / .700 / 116.742 / 1.000
Intercept / 29403.149 / 1 / 29403.149 / 1025.755 / .000 / .954 / 1025.755 / 1.000
a / 940.951 / 4 / 235.238 / 8.206 / .000 / .396 / 32.826 / .997
b / 1602.653 / 4 / 400.663 / 13.977 / .000 / .528 / 55.910 / 1.000
a * b / 802.795 / 16 / 50.175 / 1.750 / .067 / .359 / 28.006 / .864
Error / 1433.245 / 50 / 28.665
Total / 34182.793 / 75
Corrected Total / 4779.644 / 74

a Computed using alpha = .05

b R Squared = .700 (Adjusted R Squared = .556)

Tests of Between-Subjects Effects

Dependent Variable: clam Ni

Source / Type III Sum of Squares / df / Mean Square / F / Sig. / Partial Eta Squared / Noncent. Parameter / Observed Power(a)
Corrected Model / 10116.621(b) / 24 / 421.526 / 20.120 / .000 / .906 / 482.870 / 1.000
Intercept / 34433.460 / 1 / 34433.460 / 1643.520 / .000 / .970 / 1643.520 / 1.000
a / 746.325 / 4 / 186.581 / 8.906 / .000 / .416 / 35.622 / .998
b / 6554.271 / 4 / 1638.568 / 78.209 / .000 / .862 / 312.837 / 1.000
a * b / 2816.025 / 16 / 176.002 / 8.401 / .000 / .729 / 134.410 / 1.000
Error / 1047.552 / 50 / 20.951
Total / 45597.633 / 75
Corrected Total / 11164.173 / 74

a Computed using alpha = .05

b R Squared = .906 (Adjusted R Squared = .861)