TableS5: AugumentedZehr reference sequences grouped by phylum, class and nifHgroup, with number matching Poly primers and number with 95% or greater protein identity to at least one NEON read.

Phylum / Class / Group / Total / Matched
Poly
Primersa / Close
to
Reads
Actinobacteria / Actinobacteridae / I / 8 / 8 / 7
Aquificae / Aquificales / I / 2 / 2 / 0
Chrysiogenetes / Chrysiogenales / I / 1 / 1 / 0
Cyanobacteria / I / 1 / 0 / 0
Cyanobacteria / Chroococcales / I / 9 / 7 / 2
Cyanobacteria / Nostocales / I / 18 / 18 / 17
Cyanobacteria / Oscillatoriales / I / 6 / 4 / 2
Cyanobacteria / Stigonematales / I / 2 / 2 / 2
Deferribacteres / Deferribacterales / I / 2 / 1 / 0
Firmicutes / Bacillales / I / 9 / 9 / 4
Firmicutes / Clostridia / I / 7 / 6 / 2
Nitrospirae / Nitrospirales / I / 4 / 3 / 3
Proteobacteria / Alphaproteobacteria / I / 50 / 45 / 50
Proteobacteria / Betaproteobacteria / I / 13 / 13 / 13
Proteobacteria / Deltaproteobacteria / I / 13 / 13 / 12
Proteobacteria / Epsilonproteobacteria / I / 4 / 2 / 0
Proteobacteria / Gammaproteobacteria / I / 33 / 33 / 30
Verrucomicrobia / unclassified
Verrucomicrobia / I / 1 / 1 / 1
Bacteroidetes / Bacteroidia / II / 4 / 2 / 1
Chlorobi / Chlorobia / II / 10 / 10 / 0
Chloroflexi / Dehalococcoidetes / II / 1 / 0 / 0
Cyanobacteria / Nostocales / II / 1 / 0 / 0
Cyanobacteria / Oscillatoriales / II / 1 / 0 / 0
Euryarchaeota / environmentalsamples / II / 1 / 0 / 0
Euryarchaeota / Methanomicrobia / II / 7 / 0 / 3
Fibrobacteres / Fibrobacterales / II / 1 / 1 / 0
Firmicutes / Clostridia / II / 31 / 3 / 2
Fusobacteria / Fusobacteriales / II / 1 / 0 / 0
Proteobacteria / Deltaproteobacteria / II / 23 / 22 / 13
Spirochaetes / Spirochaetales / II / 4 / 3 / 0
Verrucomicrobia / Opitutae / II / 2 / 2 / 1
Verrucomicrobia / Verrucomicrobiae / II / 1 / 1 / 1
Bacteroidetes / Bacteroidia / III / 1 / 1 / 0
Chlorobi / Chlorobia / III / 1 / 1 / 0
Euryarchaeota / Methanobacteria / III / 5 / 0 / 0
Euryarchaeota / Methanococci / III / 11 / 0 / 0
Euryarchaeota / Methanomicrobia / III / 3 / 0 / 2
Firmicutes / Clostridia / III / 12 / 2 / 2
Proteobacteria / Alphaproteobacteria / III / 9 / 8 / 1
Proteobacteria / Gammaproteobacteria / III / 4 / 4 / 2
Thermodesulfo-
bacteria / Thermodesulfo-
bacteriales / III / 1 / 0 / 0
Verrucomicrobia / Opitutae / III / 1 / 1 / 1

TableS6:AugumentedZehr reference sequences grouped by phylum, class and nifHgroup, with number matching Poly primers and number with 95% or greated protein identity to at least one NEON read.

a Up to 2 mismatches for forward and reverse primers.