BIOLOGY 204 ANTELOPE VALLEY COLLEGE

GENERAL MICROBIOLOGY FALL 2007

INSTRUCTOR: Dr A. Hemsley

THEORY TEST 1 100 POINTS

STUDENT NAME: ______

STUDENT I.D. NUMBER: ______

• Perusal time = 10 minutes

• Test time = 90 minutes

• ANSWER ALL QUESTIONS

• Read ALL questions carefully during perusal time, you may write if you wish.

• Use the back of each sheet if you do not have enough writing space, but please label each answer.

• Question subheadings are NOT of equal points value.

• Points allocated for each subheading are shown at the end of the question.

GOOD LUCK!

QUESTION 1 (Total =10 points)

Match entries in the left column with answers from the lettered choices in the right column. Write your letter choice to the far left of each question in the spaces provided. Use each answer only ONCE. (1 Point per correct pair)

_____ 1. Physician who developed the first vaccine a. Lavoisier

_____ 2. Microbiologist who discovered antibiotics from soil microbes b. Hooke

_____ 3. Theory proposing that life forms could only arise from c. Edward Jenner

pre-existing life forms

_____ 4. Microbiologist whose “heat plus exclusion” experiments supported d. Prokaryotes

the Biogenesis theory

_____ 5. Scholar whose microscopy studies led to the Cell theory e. Plants

_____ 6. Scientist who studied free living microbes under the microscope f. Biogenesis

_____ 7. Kingdom whose members have a cellulose cell wall g. Protists

_____8. Scholar who believed oxygen was needed for spontaneous h. Dubos

generation

_____ 9. Kingdom whose members have a peptidoglycan cell wall i. Spallanzani

_____ 10. Kingdom whose members are all single celled Eukaryotes j. Leeuwenhoek

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QUESTION 2 (Total =10 points)

a. Discuss the significance of Joseph Lister’s work. In your answer, explain the state of medical science in Lister’s day and how his practices led to an improvement in human survival and well-being. (5)

b. Describe the classical experiment performed by Pasteur that proved the Biogenesis theory (3)

c.  Termites, cows and rabbits eat only plant matter. Since none of these animals are able to digest plant

fiber (i.e. cellulose), how do you suppose they get nutrition from the leaves and wood they eat? (2)

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QUESTION 3 (Total =20 points)

A.  Give a brief description of the following terms (2 points each): (6)

Orbital

Isotope

Synthesis reaction

B.  Pyruvic acid, generated in carbohydrate metabolism, has the following chemical formula:

O Atomic weights H = 1

O C = 12

CH3 C C O = 16

What is the molecular weight of Pyruvic acid ? Show your working. (4)

What is the weight of 4 moles of Pyruvic acid? Show your working. (5)

How many molecules are present in 2.5 moles of Pyruvic acid? (Avogadro’s number = 6.02 x 10 23) (5)

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QUESTION 4 (Total =20 points)

A.  Compare and contrast prokaryotic and eukaryotic cells by providing at least four features that are different in each type of cell ( Hint: e.g. Feature 1 might be a nuclear membrane ) (4)

B. Name and describe two anatomical features of a bacterial cell that might help it to resist the effects of antimicrobial drugs. (8)

C. Give at least 2 examples of negative impacts and at least two examples of positive impacts that microbes exert on human society. Be sure to tell me which group are negative and which are positive! (8)

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QUESTION 5 (Total =20 points)

Fill in the blanks in the following sentences. (1 point per correct sentence)

1. The 3-dimensional shape of a protein is known as its ______structure.

2. The ______is an example of a secondary structure found in protein molecules.

3. An example of a carbohydrate monomer is the substance ______.

4. ______are the polymeric molecules whose subunits contain a phosphate group, an

organic base and a 5 carbon sugar.

5. One function for lipids Is ______.

6.  ______is a waxy lipid found in the wall of bacteria that cause tuberculosis.

7. The ______stain is used to identify bacteria that cause tuberculosis .

8.  Antibiotics are produced by microbes such as ______.

9. ______microscopy is the commonest method for viewing stained specimens.

10. ______microscopy views bacteria such as Borrhelia burgdorferi illuminated against a dark background.

11. ______microscopy uses ultraviolet light instead of visible light.

12. ______electron microscopy visualizes internal cellular contents In thin tissue slices.

13. ______uses two light beams and often computer-colorizes resultant images .

14. Two genera that form endospores are ______and ______.

15. ______lenses focus the X-rays used by the electron microscope

16. Bacteria that use simple inorganic substances and chemical energy to synthesize their organic requirements are called ______

17. The energy producing potential of nutrient molecules is related to the number of ______atoms that they possess.

18. Sequential oxidation/reduction reactions are known as ______reactions.

19. The difference between anaerobic and aerobic respiration lies in the type of ______.

20. In the metabolic process known as ______, the terminal electron acceptor is an organic molecule.

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QUESTION 6 (Total =20 points)

A. Name and describe 3 Influences on the rate of an enzymatic reaction (6)

B. Describe the difference between anabolic and catabolic reactions and give an example of each type that occurs in the cell. Be sure your answer makes it clear which reaction type you are discussing! (6)

C. How does FERMENTATION differ from RESPIRATION? (4)

D. What differences are we likely to see in the growth patterns of microbes using respiration and fermentation? (4)

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