SupplementalTable 1: Sequence information of 120 SSRs developed from the pear genome

SSR locus / Forward primer sequences (5′→3′) / Reverse primer sequences(5′→3′) / Scaffold number
NAUpy01i / GTCCAGGTGATTCATCCATCTCTC / TAAGAATTGAACCATCACACCCCT / scaffold12.0
NAUpy02i / AGGCCTATTGTTGTATTTTGACCG / TATGATGATGACCCCGATCCTACT / scaffold10.0
NAUpy02z / CATAATCCGTCTGTTGGGCTTTAC / CTCCTCCACCGTCTCTATTTCTCA / scaffold912.0
NAUpy03i / CACCTAAAACAATTCCAAACGAGC / TCTTGAGAGTTGAGACTTATGTGCG / scaffold25.0
NAUpy04i / TGGAGGTAGATAGTCATCCCCCTA / GCATGTGATTTTGTTCTCTTCACG / scaffold378.0
NAUpy05i / CCACTTGTGAGGAGTGTAAAATGAGA / GCATGCATGGATATATACAGATTCAAA / scaffold355.0
NAUpy06d / CACTCACCATCATCCTCCATT / GATGCGTGGGACCCTTAT / scaffold426.0
NAUpy06i / TTAATATTTCCGGCAATTGCAAAG / CTTGGTACCCTAGTCTCTAACTCCCA / scaffold70.0
NAUpy07i / CGTATCAGTGAGTGGGTTTTTGTTT / ATCAACGATATTTGACATGGCATT / scaffold92.0
NAUpy07u / CAGCACATGGTTGGTTTAGTGTAT / TGAAACTCTTTCATATGGAGGTCA / scaffold280.0
NAUpy08i / TCAAAAGTGAGGGCAAAATAGGAA / CGCAAAACAAACATGAGTAGACCA / scaffold8.0
NAUpy10c / ATTGTATCATCGAAGCCTGGTC / TCCTTGACGACGATAGTGATTGT / scaffold247.0
NAUpy10i / GTTTTCATAAAACTTTGGGGGTTAGT / ACTCGACTCTAAATCCTGAGAGGG / scaffold12.0
NAUpy11i / AGCACTTGCATTTCATTTTGAGGT / AAAAACAAGATTCTAACCCCTCGC / scaffold55.0
NAUpy12c / GAAGGTATGTGGCAAAATCAGTAAC / TCCTAATGAGAATCCAAGTCCCCT / scaffold261.0
NAUpy22c / AACTCAGGCACAATTTCTGCAACT / TGCATTACTTTCCACTGACGTTAC / scaffold83.0
NAUpy23f / CAAATTTGAAATGTAAAAATCGCA / TCACATGAATTATATCTCACCATCAGA / scaffold226.0
NAUpy24c / ACTACAAGCAACTTCCGAGTTGAT / GCAGGAGGAAATTGTAGATGAGAC / scaffold1009.0
NAUpy27c / TCTTTGTGTTTGTGTGTGGCGTG / TGGGACTGGGATCGCTGG / scaffold139.0
NAUpy29u / AGGGATTACGTTTGGGTGAGATTT / CGAAATGGGCCTTAAAAATGACTT / scaffold88.0
NAUpy30c / GAAGGGCTGCGGTTGCTG / TCCCCTCCCTGCTCATCACTC / scaffold516.0
NAUpy31a / TCTGACGCCCGAAAACTT / GTTTTGGTGGGTCATTATCCT / scaffold3.0
NAUpy31t / GGAGCGGGTATATCTCTAGTGCAA / TTTTACTAGGCTTTCTCTTTAGGCGA / scaffold68.0
NAUpy32c / CAAACTTCTCAGCCGCATC / AGAGACCCCATAGAAGCAGTTT / scaffold234.0
NAUpy33v / TGTCAGTTGATTCATGGCCAATAG / TGATACAATTCAATTCCAGGGACC / scaffold1231.0
NAUpy35c / ATTGTGTACTATCCGTGTTCCTGT / TGATCCCCATTTTATCTCCGT / scaffold581.0
NAUpy35v / TCATCAAACGTTAATGCCAATAAGAA / ACTTTTGTTTGGATGGAAACGATG / scaffold669.0
NAUpy36t / ATCTGTGAGTGGGCACTTTGTTTT / TCAACCACATAACAGCAATTTTGAA / scaffold746.0
NAUpy38c / GTGCAGATCACATGGCAGAGACG / AGAAGAAAAAGGAGGGAGGGGAG / scaffold51.0
NAUpy38e / GCTGCTTGCTGCAGATCAAGT / CGAAAAGCTCAGAGTATCTTACACTCG / scaffold1199.0
NAUpy39h / ACATAAATAACCATTTTTCAGAGCACAA / ATAACTCATGTGAGGGTGATGCAA / scaffold12.0
NAUpy40c / CAATGAAACCCCAGAAGCC / TGAAGGGGAGCAGAGTGAAG / scaffold242.0
NAUpy41v / TGTCTCTCAGTGTTCCCTTTCTCC / CCACTGAAATGGATTGTAATGGATG / scaffold416.0
NAUpy43c / TTGGCGTTACTGATACTTCGTC / AAAACCGACTTGTGAAACCTCT / scaffold1.0
NAUpy45f / CAATAAAAGGAAGCCACATAGAATCA / CCATCATTTGGAAGAAAGAAACAC / scaffold181.0
NAUpy46k / TTAATCCATGGTTGGAGACTCAAG / AAATCATTGGGCACAATTTGATAA / scaffold389.0
NAUpy14d / TGTGATAGTGTTTGGGCTACGAC / GGCTAATGTCATAACAATAAAAGAAG / scaffold199.0
NAUpy48t / ACTATTGAATGGGTTTGAATTTTGA / ACTCTTTGATAGGGTGCATTTTTG / scaffold821.0
NAUpy53a / TAAACAGATACCCCGCACAT / TGACTGCCATTGCTCGGT / scaffold136.0
NAUpy55k / CACATGCACGCGCTATACAAATA / CCACGCTGACGATCAGTTAGAGATA / scaffold513.0
NAUpy56h / TGTCGTTTCCTTGAGTAGGAGAAA / AAAAAGATTGCGTAAATAGACTGAACTC / scaffold159.0
NAUpy58t / AGCCCAATTAAAGGAGAAGCAAGT / CCAATATTGCCCATGAGTTTGAA / scaffold482.0
NAUpy60e / GGGACACAGGAGGTTGAAAGTT / AAATTTTAATATATCCTCCCAATCAGGT / scaffold96.0
NAUpy16d / CACAAAATGGGTTGGAAGAATG / CACCCTCCTCCTCGTCCTC / scaffold17.0
NAUpy64h / TTAGAATTTGTTGCCGAGATTGTG / CAGATTGTATGATGAGCTGGCACT / scaffold382.0
NAUpy64t / AATCTGCACGCTTTGAAGATGAAC / ATATAGTTTGCATGCCGTGTGGAT / scaffold1208.0
NAUpy66w / ATGATTCTCACTCCTTCGCAGAAC / AACATATACATTCGTTTTGGGGCA / scaffold1105.0
NAUpy67h / CATGGCCTAGGTTGGCTCATATT / ATCAAAGCCCAACTAATTTCCCAT / scaffold520.0
NAUpy67t / CTTCAATTAGCCCTCCATTAACCC / AACAAGAAGACGACGACTTGGAAT / scaffold546.0
NAUpy68h / TGTGCACCTAATCACAATGTAATGAAA / TATCTTGGCGAGTAAGGAATTGGA / scaffold18.0
NAUpy68n / TTTAACAGCAGCACAGGCAGTTTA / TCAGACATGATTCAACATGGAAGAA / scaffold965.0
NAUpy69h / TCATCGTTGAATTGCATGCTTTAT / TTGTCTGTTCTCCCTGGCAAGTAT / scaffold245.0
NAUpy70h / GCGTCTAGGGCTTTACAGGAAAAA / AATTTTGGAGGGAGGAAGAAAGTG / scaffold174.0
NAUpy71h / AATGACCAAAACTATATGGCCCAA / TGAAAGCACCAATCTTTTCTTGGT / scaffold607.0
NAUpy71v / CAATCCAACATTTCATTCCAAACA / TCAAACAAAGACTCACATTCAAACAA / scaffold137.0
NAUpy17d / AGGTAATGACAGGGAATGGATG / AGGCTGATGAGGGGACTTTC / scaffold723.0
NAUpy72h / TTAGCATTGCTTACCATTCCCTGT / GACGATTGGCTTTTTGATTTTGTT / scaffold40.0
NAUpy73h / TTGCCTCCTCTCCACTCAATAGTT / GATGAATGGATGTGATTGGAGGAT / scaffold48.0
NAUpy73m / TCTCCAGAAGTTTATTTGCACAACTT / TGCATCTAAATTCACCCATTCTCT / scaffold25.0
NAUpy74f / ATTGCCATGTGGTAGTCTCTCCTT / TAGGGGGTTTGAGAGAAAAAGAGG / scaffold190.0
NAUpy74h / GCGAGAGCGTAGGAGAAATCTTTT / GTAGCGTGACCACAAGACAATGAA / scaffold65.0
NAUpy74v / GTTTTTCATGCTTGGATTTTGCTC / CCATGGGTTACCACTTAAACTTGC / scaffold803.0
NAUpy75h / TGTTGTTGGTAAAGAAAATTGTAGAAAA / TTTTCTTGTTTGATGAGATCCCC / scaffold413.0
NAUpy75t / CTAAAACTCTTTGTCCAGCCCTGA / GCGAGTAATGAGGTTGTATAAGTCTGG / scaffold877.0
NAUpy76h / AAAAGCGACCAAAGAATTCAGAGA / GGAGACTTGTGGCTTTGAACTGAC / scaffold12.0
NAUpy77h / TTGGCCTATATATGAAATATGGTGGG / GCCTCATGCACGGTTTTTACAT / scaffold143.0
NAUpy79h / TCGAAAATTGGATAAACAGATTGAAA / TTGTTCGGCGTCGTATTTAGTATT / scaffold192.0
NAUpy80h / TGTCTTCTGCTTCATCTTCTTCAAGT / ATTGGCGTACAGAGGAAGAGCTAA / scaffold590.0
NAUpy80k / CATTGAGAACTTCAAATGTTTCTCCTC / GAGGATTTCCAACTTCACGTTTTG / scaffold408.0
NAUpy81c / ACAATCTCAGCGACAATTTCTAT / GGGAAAGCCCATCTCGGT / scaffold151.0
NAUpy81h / TTTTAACCTACGATGGAAATAATGTCA / TCCTCAACCCTAGTCTTTTTGTTTG / scaffold97.0
NAUpy81m / AGTGGCTAAAAATGGTGCGTGTAT / GGGCTATCTTCTCTCTTGTTGCTG / scaffold171.0
NAUpy85c / TAAACGACAACAGCGAGGGT / ACGAAGGCAGCAAGGAGCA / scaffold3.0
NAUpy85d / ATCTTCTCCACCCTCTTCCG / ACCTTCTTCCCAGCACCTT / scaffold128.0
NAUpy85h / GATGGAGTGTACAACAGCTTGCAT / ATGATTGGGATATGTTTGATGCCT / scaffold654.0
NAUpy85x / TCCGGAAACCATTTTAATTTCACA / ATAGTTCCGATTGAAGATCGCTTG / scaffold189.0
NAUpy86c / AATTGTTCAACCAGTGTTCATCTAT / CTGGCTCCGGCTACCTTAC / scaffold57.0
NAUpy86h / TGAGTGCACTTTTTGTTTTCGTGT / TGGCAGGATATGCATAAATCAATTC / scaffold65.0
NAUpy86n / TTGGAGGTCAAGCATAAAATAGGC / AAAGAAAAGAAGGATAGGCAATGGT / scaffold45.0
NAUpy87c / GTTGCTCTTCCCATTCTACAGG / ATTCCGCTACATCAGGGGT / scaffold94.0
NAUpy87d / CGACCTTATCTTCTTAAATCTGTG / CACCGCAAGGTTTTCTCG / scaffold1067.0
NAUpy87h / CATATTAGTGAGTGGGCATTTGTTT / TCGAAGAATGCAAAACTAAAAGTAAGA / scaffold75.0
NAUpy88h / TTTAGGTGGTTCCGACTCGTAGGT / AAGTGGTCTGTACGACAGGACTGG / scaffold127.0
NAUpy88x / GGTTTCGATCCATTGATGTTTTTC / TTTCTCTCCATCGTTACTTTCTGATG / scaffold528.0
NAUpy89c / AAGTCCAAAATATTCAAAGGCT / TCTTGTTCTTGAGATGGAGGAG / scaffold1104.0
NAUpy89h / GATTTTCCTTGATTATAACACCATCAAT / TGTCCGTTATGTTGTAACTCCATATTT / scaffold630.0
NAUpy90w / GGCACAATAAAACAAAATTCATTGC / TATTTTGGCCAACCTGTGACG / scaffold243.0
NAUpy91b / CAGTCCGTTGGAGCAAGTTATC / CGGTTCTTTGGGTTGTGCTT / scaffold13.0
NAUpy91h / AATTATGACTATTGGTCGCGCTTG / ACATGTACCATCTTTTGGAACATACAGA / scaffold288.2
NAUpy91t / CTAGCTCCGAAACAGAGGAAAGGT / CGCCCTAACCCTATTTACTTCTGG / scaffold437.0
NAUpy92a / GGTAGGCTTCGCTTGGAGG / AAAGCCAATAGAGAATCCGCAAG / scaffold140.0
NAUpy92e / TTCAAAACCCAAGTTCACCTTAGC / CGATGGCAGTTAAATTGTGTCAAA / scaffold816.0
NAUpy92h / GACATGGCAAGCAAAGCATAATTC / GGGGCTACGATCCTGCTACTTATC / scaffold236.0
NAUpy92x / GCACCAACCTCACTCTTCCTTTTA / CCCGTTCCATTTCTATACCATGTG / scaffold371.0
NAUpy94h / GACAGCTTAAACAATAATCTTTGCCC / TTGTTGGAAATGTGCCCTAAAAGT / scaffold442.0
NAUpy94x / TCAATTCCATCATCACCTTTTGAA / CAGTTTAGACGATGAAACCTGGCT / scaffold250.0
NAUpy95h / ATTGACTTACACGGATGTTGCCTT / GAATCACCATGCATATCTTGAGACC / scaffold20.0
NAUpy98h / CAGGGTCTAGGCGAATTGAACTTA / AGCACACATTATATGCTTGTTCCCT / scaffold115.0
NAUpy98s / AATCAAACATCTTCAAGTCCCCAA / CCTGCTCATCCATGCACATATAAA / scaffold24.0
NAUpy98x / GAGGACGTACGAGGATAATTGAGG / TCAATTTAAAAGCATTTGTGGAAACT / scaffold115.0
NAUpy99h / ATTAAACTTCCCACCTCCCAACTC / GAAAGTAGGATTGTGTTCGGGCT / scaffold166.0
NAUpy18d / CCTTTGGGTGGGTGCTGT / TTCTGAAAACCCTAGCATACCAT / scaffold12.0
NAUpy30u / AGAAGGAAGACGAAGAAGCTCACA / AAAACGCTTAATTAGACGGCGAC / scaffold507.0
NAUpy62u / TCCGATTTTATCTCACATTCCCAC / TCGACGACTAAAAGTCAACAGTGC / scaffold219.0
NAUpy43w / CAGCGCTTACTAATCTCTTCCCAG / TCTCAGAAACCAAACAGATTGCAG / scaffold282.0
NAUpy11d / ACCCTCCCTCCATTGTTGTT / GGGGGGCTTGGCTAATGG / scaffold1.0
NAUpy13d / ATTATGTGCCTTGATTCTAACTCTTG / GGCGGCGACATCACCAAT / scaffold266.0
NAUpy21d / GCCACCTGTTTGCGTTGTATC / GGATGCGGGTTTCTGTGG / scaffold186.0
NAUpy22d / CCCACCCTATTCTTCACCG / TTCAGAAGCCATTTCCATCC / scaffold203.0
NAUpy23d / CCGCAGGAGGAGGACTACC / GGCAAGTTAAAATTGGGGTG / scaffold513.0
NAUpy24d / AGTGTCACGCAATGGAGCC / GCCAATTCTATTCTTTCAAAACCTG / scaffold8.0
NAUpy25d / AGGGTCGGAGGAAGAGGAT / TGGCGTCTCCATTTTCTTCT / scaffold40.0
NAUpy27d / CTGATTTGAACAAGTGCGAACCT / CCCGTCGGATACTTCCTGTC / scaffold809.0
NAUpy28d / AAACAAGGGAAAGGATGACAAG / CCTCCTTACCGTTTCTTCATTGG / scaffold1057.0
NAUpy29d / GCTTGTGGTGCTGTTTACTTGT / TTGATAGTGGAAAACAAGAGGTG / scaffold1057.0
NAUpy30d / AAACGGAGGAACCCACAAT / GCCACAGAATGCACCTTTACC / scaffold353.0
NAUpy33d / ACCAAGACTACTACTCTCAAAGCAT / AGTTCAGATTCGAGGAAAGCAAG / scaffold190.0
NAUpy34d / ATAAACAACCGACTGAGCAACAT / TCCATCCAAGTAAAGTGCTCAG / scaffold926.0
NAUpy35d / GCCTGAAAACTCCCAAGACC / GACATCCATCACTCGTATTTGC / scaffold85.0
NAUpy06z / AATTCCATCATTTCGCTAATTCTCA / TCAGGTGAGTGGGTATTTTCCTTT / scaffold1351.0

Supplemental Fig. 1: Amplification of pear SSR markers NAUpy27c, NAUpy35v, NAUpy98s, NAUpy80k, NAUpy85d, NAUpy43c in apple accessions. Lanes:M1, 100bp ladder DNA marker; M2, pBR322 DNA marker; 1, ‘Gala’; 2,‘Qinyang’; 3,‘HoKuto’; 4,‘Jonagold’; 5, ‘Orin’; 6, ‘Lujia 1’;7, ‘Alps Otome’;8, ‘Hanfu’;9 ‘Xiushui’

SupplementalFig. 1